TWI663989B - Composition containing natural plant extracts, external skin preparation comprising the same, cosmetic preparation comprising the same and pharmaceutical preparation comprising the same - Google Patents

Abstract

The invention relates to a composition containing cold blue extract and at least one extract selected from the group consisting of green tea extract, camellia flower extract, paeonia lactiflora extract and nopal cactus extract as an active ingredient, and a composition containing the composition Skin external preparation, a cosmetic preparation containing the composition, and a pharmaceutical preparation containing the composition.

Description

Composition containing natural plant extract, skin external preparation containing the same, cosmetic preparation containing the same, and pharmaceutical preparation containing the same

The invention relates to a composition containing a natural plant extract as an active ingredient, a skin external preparation containing the same, a cosmetic preparation containing the same, and a pharmaceutical preparation containing the same. More specifically, the present invention relates to extracts containing Cymbidium kanran and green tea extract, camellia japonica extract, Bletilla striata extract, and Opuntia Coccinellifera extract. At least one kind of extract selected from the group is used as an active ingredient to have anti-oxidant and anti-inflammatory effects.

Inflammatory skin diseases include atopic dermatitis, contact dermatitis, and seborrheic Dermatitis, acne and similar diseases.

In general, the mechanism or stimulus response or inflammation involves various cytokines released by human keratinocytes or Langerhans cells in response to external substances. Cytokines, accelerators of the human immune system, are necessary during skin irritation or local inflammation (J Appl Toxicol 16: 65-70, 1996). In fact, it has been reported that in the case of contact dermatitis, interleukin-6 (IL-6), interleukin-1 (IL-1) or tumor necrosis factor alpha, TNF-α) increased (Contact Dermatitis 35: 355-60, December 1996).

Conventionally, antihistamine drugs, vitamin ointments or adrenal cortex drugs have been used to treat the inflammatory skin diseases, but most of them have a temporary effect and in most cases even have side effects.

Therefore, there is a need to develop a composition that effectively treats diseases related to oxidation or inflammation occurring in the skin, contains natural plant extracts as an active ingredient, and causes relatively few side effects compared with hormone preparations.

Previous literature

Patent literature

(Patent Document 1)

Korean Patent Licensing Publication No. 10-2013-0031988 (April 1, 2013)

It is an object of the present invention to provide a composition containing various natural plant extracts as active ingredients and having anti-oxidant and anti-inflammatory effects, and comprising the group The skin external preparations, cosmetic preparations and pharmaceutical preparations.

The invention provides a composition containing cold blue extract and at least one selected from the group consisting of green tea extract, camellia flower extract, white peony extract and nopal cactus extract as an active ingredient.

The present invention also provides a skin external preparation containing the composition.

The present invention also provides a cosmetic preparation containing the composition.

The invention also provides a pharmaceutical preparation comprising the composition.

The role of the invention

The composition of the present invention contains at least two natural plant extracts as active ingredients, and therefore has a relatively good anti-oxidant effect of removing active oxygen and good skin relief compared with a composition containing a single natural plant extract as active ingredients Anti-inflammatory effect of inflammation.

FIG. 1 is an illustration showing the evaluation results of TNF-α according to the respective compositions of Comparative Example 1 to Comparative Example 5 and Examples 1 to 5 in Experimental Example 1. FIG.

FIG. 2 is an illustration showing the results of IL-6 evaluation according to the respective compositions of Comparative Example 1 to Comparative Example 5 and Examples 1 to 5 in Experimental Example 2. FIG.

The invention provides a composition containing cold blue extract and at least one selected from the group consisting of green tea extract, camellia flower extract, white peony extract and nopal cactus extract as an active ingredient.

In the present invention, cold blue refers to a cold blue plant of the Orchidaceae family.

In the present invention, green tea refers to a product made from the buds or leaves of a Camellia Sinensis plant by exposing the oxidase present in the tea leaf to heat or steam to activate the enzyme.

In the present invention, the camellia means a camellia plant which is a tea tree species.

In the present invention, Paeonia lactiflora refers to Paeonia lactiflora which is a species of Orchidaceae.

In the present invention, nopal cactus refers to a nopal plant which is a species of the cactus family.

Cold blue extract, green tea extract, camellia extract, paeonia lactiflora extract, and nopal extract can be prepared according to various preparation methods known in the related art, that is, using known solvents under known temperature and pressure conditions. Each extract is preferably extracted with an extraction solvent selected from the group consisting of: water, anhydrous or water-containing lower alcohols having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, glycerol, 1, 3-butanediol, 1,2-propanediol, and 1,3-propanediol.

Cold blue extract can be extracted from the whole plant of cold blue. Amaranth extract can also be extracted from the entire plant of Amaranth.

Green tea extract can be extracted from the leaves of the tea plant and camellia extract can be extracted from the leaves of the camellia plant.

Nopal extract can be extracted from the fruits of nopal plants.

In one embodiment of the present invention, the composition contains cold blue extract and green tea extract as active ingredients.

Since it contains a mixture of cold blue extract and green tea extract, the composition using at least two natural plant extracts as active ingredients can increase its antioxidant effect Strong maximization.

In one embodiment of the present invention, the composition contains Hanlan extract and camellia extract.

Due to the composition containing cold blue extract and camellia extract, the composition using at least two natural plant extracts as active ingredients can maximize the enhancement of its anti-inflammatory effect in the TNF-α inhibited state.

In one embodiment of the present invention, the composition contains Hanlan extract and Paeonia lactiflora extract.

Due to the mixture of Hanlan extract and Paeonia lactiflora extract, compared with the composition containing a single natural plant extract, the composition can relatively maximize the enhancement of its anti-inflammatory effect in the TNF-α inhibited state .

In one embodiment of the present invention, the composition contains Hanlan extract and nopal cactus extract.

Due to the mixture of Cold Blue Extract and Nopal Cactus Extract, compared with the composition containing a single natural plant extract, the composition can relatively maximize its anti-inflammatory effect in the IL-6 inhibited state. Into.

In one embodiment of the present invention, the composition contains cold blue extract and green tea extract.

Because it contains a mixture of cold blue extract and green tea extract, a composition containing two natural plant extracts as active ingredients can maximize the inhibition of TNF-α and IL-6 to maximize its anti-inflammatory effect.

In one embodiment of the present invention, the composition contains green tea extract, cold blue extract, camellia extract, paeony extract and nopal extract as active ingredients.

By using a mixture containing green tea extract, cold blue extract, camellia extract, paeony extract and nopal extract as active ingredients, the composition can maximize its anti-inflammatory and antioxidant effects.

In one embodiment of the present invention, the composition comprises 0.0001% by weight or more than 0.0001% by weight and 10% by weight or less with respect to the total weight of the composition, and more specifically, 0.01 Cold blue extract of 0.01% by weight or more and 5.0% by weight or less. A content of 0.0001% by weight or greater of Khan Blue Extract can provide antioxidant and anti-inflammatory effects from Khan Blue Extract. The content of 10% by weight or less of the cold blue extract is more effective by relatively enhancing the antioxidant and anti-inflammatory effects from the cold blue extract in relation to the increment.

In one embodiment of the present invention, the composition comprises 0.0001% by weight or more than 0.0001% by weight and 90% by weight or less than 90% by weight of the green tea extract relative to the total weight of the composition, more specifically, 0.01 Green tea extract of 0.01% by weight or more and 90% by weight or less. A content of green tea extract of 0.0001% by weight or more can provide antioxidant and anti-inflammatory effects from green tea extract. The content of green tea extract of 90% by weight or less is more effective by relatively enhancing the antioxidant and anti-inflammatory effects of green tea extract relative to the content increase.

In one embodiment of the present invention, the composition comprises 0.0001% by weight or more than 0.0001% by weight and 90% by weight or less than 90% by weight of camellia extract with respect to the total weight of the composition, more specifically, 0.01 Camellia extract of 0.01% by weight or more and 90% by weight or less. Camellia extract content of 0.0001% by weight or greater than 0.0001% by weight can be derived from camellia extract Antioxidant and anti-inflammatory effects of extracts. The content of the camellia extract of 90% by weight or less is more effective by relatively enhancing the antioxidant and anti-inflammatory effects of the camellia extract relative to the content increase.

In an embodiment of the present invention, the composition comprises 0.0001% by weight or more than 0.0001% by weight and 10% by weight or less than 10% by weight of amaranth extract with respect to the total weight of the composition, more specifically, 0.01 Extract of Paeonia lactiflora in weight% or more than 0.01 weight% and 5% or less. The content of Paeonia lactiflora extract of 0.0001% by weight or more can provide antioxidant and anti-inflammatory effects from Paeonia lactiflora extract. A content of 10% by weight or less of Paeonia lactiflora extract is more effective by relatively enhancing the antioxidant and anti-inflammatory effects of Paeonia lactiflora extract relative to the content increase.

In one embodiment of the present invention, the composition includes nopal extract from nopal, 0.0001% by weight or more than 0.0001% by weight and 90% by weight or less than 90% by weight with respect to the total weight of the composition, more specifically, 0.01% by weight or more of 0.01% by weight and 90% by weight or less of 90% by weight of nopal extract. The content of nopal extract of 0.0001% by weight or more can provide antioxidant and anti-inflammatory effects from nopal extract. A content of 90% by weight or less of nopal extract is more effective by relatively enhancing the antioxidant and anti-inflammatory effects of nopal extract from the content increase.

In one embodiment of the present invention, the composition is used as an antioxidant composition. The antioxidant composition uses at least two extracts as active ingredients to eliminate or inhibit free radicals in the skin.

In one embodiment of the present invention, the composition is used as an anti-inflammatory composition. Anti-inflammatory composition uses at least two extracts as active ingredients to inhibit or reduce Interleukin-6 (IL-6), interleukin-1 (IL-1) or tumor necrosis factor alpha (TNF-α).

The present invention provides a skin external preparation comprising the composition listed above.

Skin external preparations broadly refer to all preparations applied to the outside of the skin and include any preparation administered by absorption through the skin, that is, any transdermal preparation.

In one embodiment of the invention, the skin includes skin around the eyes.

In one embodiment of the present invention, specific formulations of transdermal formulations include (but are not limited to) powder formulations for external use, lozenge formulations for external use, liquid formulations for external use, patches, microneedles , Ointment or suppository.

The present invention provides a cosmetic comprising the composition listed above.

The cosmetics can be formulated using cosmetically or dermatologically acceptable media or matrices. The cosmetic refers to any kind of cosmetic formulation suitable for topical application, including, for example, solutions, gels, solids, pasty anhydrous products, oil-in-water emulsions, suspensions, microemulsions, microcapsules, microparticles, Ionic (liposomal) or non-ionic capsule dispersants, creams, toners, lotions, powders, ointments, sprays or concealers. The cosmetics may also be provided in the form of a foam composition or an aerosol composition additionally containing a compressed propellant. The cosmetic may be prepared according to a method known in the related art.

In addition, the cosmetics may contain lipid substances, organic solvents, solubilizers, concentrates, gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, flavoring agents, surfactants, water, ions or Non-ionic emulsifiers, fillers, clamps, chelating agents, preservatives, vitamins, blockers, humectants, essential oils, dyes, pigments, hydrophilic or lipophilic activators, lipid vesicles, or generally used in cosmetics or Any other adjuvant in the field of dermatology, such as other ingredients commonly used in cosmetics Minute. Adjuvants can be used in cosmetically or dermatologically acceptable amounts.

The present invention provides a pharmaceutical formulation comprising the composition listed above.

The composition may additionally contain pharmaceutical adjuvants, such as preservatives, stabilizers, water-dispersible powders, emulsifying accelerators, salts and / or buffering agents for controlling osmotic pressure, etc., and are suitable for treatment and formulated into various kinds according to general methods Other substances in oral or parenteral dosage forms.

Examples of oral dosage forms include lozenges, pills, hard or soft capsules, liquids, suspensions, emulsions, syrups, granules, etc., which contain diluents such as lactose, dextrose, sucrose, mannose in addition to the active ingredient Sugar alcohols, sorbitol, cellulose, and glycine), lubricants (such as silicon dioxide, talc, stearic acid and its magnesium or calcium salts, and polyethylene glycols). Lozenges may further contain binders such as magnesium aluminum silicate, starch paste, gelatin, tragacanth, methyl cellulose, sodium carboxymethyl cellulose, and polyvinylpyrrolidone, and in some cases, pharmaceutical additives , Such as disintegrants (eg, starch, agar, alginic acid or its sodium salt, etc.), adsorbents, colorants, flavoring agents, sweeteners, and the like. Lozenges can be prepared by general methods including mixing, granulating or coating. In addition, representative examples of parenteral dosage forms are formulations for injection, preferably an equal proportion of an aqueous solution or suspension.

In the following, the construction and function of the present invention will be described in more detail with reference to experimental examples and examples, which are provided only to help better understand the present invention and are not intended to limit the present invention. category.

<Comparative Example 1> Preparation of Cold Blue Extract

950 grams of purified water was added to the whole plant of 50 grams of cold blue, followed by extraction with hot water at 80 ° C. for 12 hours. The extract thus obtained was filtered through a 1 micron filter and concentrated with an evaporator until the amount of the extracted liquid reached 3.5 g To prepare a sample. In each of the following Experimental Examples 1 to 3, 20 micrograms of Hanlan extract was used.

<Comparative Example 2> Preparation of Paeonia lactiflora extract

The whole plant of 20 grams of Amaranth was mixed with 80 grams of a solvent mixture of purified water and glycerol (weight ratio of 3: 7) and extraction was performed at 25 ° C for 3 days. The extract thus obtained was filtered through a 1 micron filter to prepare a peony extract. In each of the following Experimental Examples 1 to 3, 20 μg of Amaranth extract was used.

<Comparative Example 3> Preparation of camellia extract

The procedure was performed in the same manner as described in Comparative Example 1, except that 20 g of camellia leaves were used instead of the whole plant of Cold Blue. 20 micrograms of camellia extract was used in each of the following Experimental Examples 1 to 3.

<Comparative Example 4> Preparation of Nopal Cactus Extract

The procedure was performed in the same manner as described in Comparative Example 1, but instead using 20 g of nopal cactus fruit instead of the whole plant of Cold Blue. 20 micrograms of nopal extract was used in each of the following Experimental Examples 1 to 3.

<Comparative Example 5> Preparation of green tea extract

The procedure was performed in the same manner as described in Comparative Example 1, but instead using 20 grams of green tea leaves instead of the whole plant of Cold Blue. 20 micrograms of green tea extract was used in each of the following Experimental Examples 1 to 3.

<Example 1> Preparation of mixed extract of Hanlan and Paeonia lactiflora

10 micrograms of Cold Blue Extract from Comparative Example 1 and 10 micrograms of White Radix Extract from Comparative Example 2 were mixed together to prepare a mixed extract of Cold Blue and White Radix Take things.

<Example 2> Preparation of mixed extract of Camellia and Camellia

10 micrograms of Camellia extract from Comparative Example 1 and 10 micrograms of Camellia extract from Comparative Example 3 were mixed together to prepare a mixed extract of Camellia and Camellia.

<Example 3> Preparation of a mixed extract of Hanoi and Nopal

10 micrograms of Hollylandia extract from Comparative Example 1 and 10 micrograms of Nopal extract from Comparative Example 4 were mixed together to prepare a mixed extract of Hollyberry and Nopal.

<Example 4> Preparation of mixed extract of cold blue and green tea

10 micrograms of cold blue extract from comparative example 1 and 10 micrograms of green tea extract from comparative example 5 were mixed together to prepare a mixed extract of cold blue and green tea.

<Example 5> Preparation of mixed extracts of cold blue, white peony, camellia, nopal, and green tea

4 micrograms of cold blue extract from Comparative Example 1, 4 micrograms of Amaranth extract from Comparative Example 2, 4 micrograms of camellia extract from Comparative Example 3, 4 micrograms of Nopal extract from Comparative Example 4, and 4 micrograms from Comparative The green tea extracts of Example 5 were mixed together to prepare a mixed extract of cold blue, white peony, camellia, nopal, and green tea.

<Experimental Example 1> Evaluation of free radicals, DPPH

In order to evaluate the antioxidant effect of the respective extracts prepared in Examples 1 to 5, via the radical 1,3-diphenyl-2-pictylhydrazyl (1,3-diphenyl-2-picryl hydrazyl, DPPH) reduction in absorbance changes Comparative evaluation of DPPH oxidation inhibition was performed to evaluate antioxidant capacity. In other words, regarding the decrease in the absorbance caused by the inhibition of the oxidation of DPPH relative to the control group, the extracts obtained in Examples 1 to 5 and Comparative Examples 1 to 5 were measured, so the absorbance is the control absorbance Concentrations of 50% or less are considered effective antioxidant concentrations. For effective antioxidant concentrations, each of the control and individual extracts were measured three times. The ratios of the average effective antioxidant concentration and the average effective antioxidant concentration of the corresponding extracts to the effective antioxidant concentration of the control group are shown in Table 1.

As shown in Table 1, when comparative example 2 and example 1, comparative example 3 and example 2, comparative example 4 and example 3, and comparative example 5 and example 4 were compared, the cold blue extract was combined with another extract Examples 1 to 4 are far superior to Comparative Example 2 to Comparative Example 5 containing a single extract as an active ingredient in terms of antioxidant effect.

In particular, Example 4 containing a green tea extract combined with a cold blue extract was compared with a green tea extract as a single active ingredient in terms of antioxidant effect. 5 is much better.

In addition, Example 5 containing Hanlan extract, Paeonia lactiflora extract, camellia extract, nopal extract, and green tea extract as various active ingredients was the most effective in terms of antioxidant effect.

<Experimental Example 2> Evaluation of TNF-α inhibitory effect

In order to evaluate the TNF-α inhibitory effect of the respective extracts prepared in Examples 1 to 5, experiments were performed on a human keratinocyte cell line HaCaT cell (provided by the Korean Cell Line Bank).

HaCaT cells were seeded in a 48-well culture plate at a concentration of 1 × 10 ⁴ cells / well, and cultured in 10% serum-containing DMEM medium (Biowhittaker, catalog number 12-604F) for 24 hours and It was further cultured for 24 hours in serum-free medium. The total protein content in the cultured cells was measured three times and averaged. The cultured cells were washed once with PBS, and 1 microgram / ml of LPS was added to the culture medium with 100 microliters of PBS in addition to the control group. After removing the PBS, except for the control group and the untreated group, each sample of the extracts prepared in Examples 1 to 5 and Comparative Examples 1 to 5 was added at a concentration of 10 micrometers in an amount of 500 microliters Put into each well. Within 6 hours after treatment, each medium was collected and the amount of TNF-α was measured three times using a TNF-α ELISA analysis kit (BD Biosciences, catalog number 555212). The TNF-α measurement values are averaged, and the calculation results are presented in Table 2 and plotted in the graph of FIG. 1.

As shown in Table 2 and the graph of FIG. 1, when comparative example 2 and example 1, comparative example 3 and example 2, comparative example 4 and example 3, and comparative example 5 and example 4 are compared, it contains another extract The combined cold blue extract Examples 1 to 4 are far superior to Comparative Example 2 to Comparative Example 5 with a single extract as an active ingredient in terms of anti-inflammatory effect.

In particular, Example 1 containing Paeonia lactiflora extract in combination with Hanlan extract was much better in anti-inflammatory effect than Comparative Example 2 containing Paeonia lactiflora extract as a single active ingredient.

In addition, in a composition containing two natural plant extracts as active ingredients, Example 4 containing a green tea extract combined with cold blue extract was the best in terms of the anti-inflammatory effect in the aspect of TNF-α inhibition.

In addition, Example 5 containing Hanlan extract, Paeonia lactiflora extract, camellia extract, nopal extract, and green tea extract as various active ingredients was the most effective in terms of anti-inflammatory effect.

<Experimental Example 3> Evaluation of IL-6 inhibition

The procedure was performed in the same manner as described in Experimental Example 2, except that the TNF-α ELISA analysis kit (BD Biosciences, catalog number 555212) was used instead to measure the amount of IL-6 in each medium instead of TNF-α Three times to assess IL-6 inhibition use. The measurement results are presented in Table 3 and plotted in the graph of FIG. 2.

As shown in the graphs of Table 3 and FIG. 2, when Comparative Example 2 and Example 1, Comparative Example 3 and Example 2, Comparative Example 4 and Example 3, and Comparative Example 5 and Example 4 were compared, they contained another extract. The combined cold blue extract examples 1 to 4 are far superior to the comparative examples 2 to 5 containing a single extract as an active ingredient in terms of the anti-inflammatory effect in the aspect of IL-6 inhibition.

In particular, Example 3 containing nopal extract in combination with cold blue extract was much better in anti-inflammatory action in the aspect of IL-6 inhibition than comparative example 4 containing nopal extract as a single active ingredient.

In addition, in a composition containing two natural plant extracts as active ingredients, Example 4 containing a green tea extract combined with a cold blue extract was the best in terms of the anti-inflammatory effect in the aspect of IL-6 inhibition.

In addition, Example 5 containing cold blue extract, white peony extract, camellia extract, nopal extract, and green tea extract as various active ingredients The IL-6 inhibitory effect is most effective in terms of anti-inflammatory effect.

Claims (6)

A cosmetic composition containing Cymbidium kanran extract and a group selected from the group consisting of green tea extract, camellia japonica extract, Bletilla striata extract, and nopal cactus extract ( Opuntia coccinellifera ) At least one extract as an active ingredient, wherein the cold blue extract is extracted from whole plant of cold blue with hot water; the green tea extract is extracted from leaves of Camellia sinensis with hot water; the camellia extract Extracted from the leaves of camellia with the hot water; extracted from the whole plant of the peony with a solvent mixture of water and glycerol in a weight ratio of 3: 7; and the nopal extract with the hot water Extracted from nopal fruit. The cosmetic composition according to item 1 of the scope of patent application, wherein the cosmetic composition contains: 0.0001% to 10% by weight of the cold blue extract relative to the total weight of the cosmetic composition; and 0.0001% by weight % To 90% by weight of the green tea extract, 0.0001% by weight to 90% by weight of the camellia extract, 0.0001% by weight to 10% by weight of the paeony extract, and 0.0001% by weight to 90% by weight of the nopal cactus extract At least one extract selected from the formed group. The cosmetic composition according to item 1 of the scope of patent application, wherein the composition is formulated using a cosmetically or dermatologically acceptable medium or matrix. The cosmetic composition according to item 1 of the scope of the patent application, wherein the composition comprises a gel, a paste-like anhydrous product, an oil-in-water emulsion, a suspension, a microemulsion, a microcapsule, a microparticle, and an ion (liposome). ) Or non-ionic capsule dispersant, cream, toner, lotion, powder, ointment, spray, concealer, foam or aerosol. A use of a composition for preparing an anti-oxidant medicinal composition, the composition comprising a cold blue extract and a group consisting of green tea extract, camellia extract, paeony extract and nopal cactus extract A mixture of at least one selected extract, wherein the cold blue extract is extracted from whole plant of cold blue with hot water; the green tea extract is extracted from leaves of tea tree with the hot water; and the camellia extract is extracted from the Hot water is extracted from the leaves of camellia; the Paeonia lactiflora extract is extracted from the entire plant of Paeonia lactiflora in a solvent mixture of water and glycerol in a weight ratio of 3: 7; and the nopal extract is noble cactus from the hot water Fruit Extract. A use of a composition, which is used for preparing an anti-inflammatory medicinal composition. The composition includes a cold blue extract and a group selected from the group consisting of green tea extract, camellia extract, paeony extract, and nopal cactus extract. A mixture of at least one extract, wherein the cold blue extract is extracted from whole plant of cold blue with hot water; the green tea extract is extracted from leaves of tea tree with the hot water; the camellia extract is extracted from the heat Water is extracted from the leaves of camellia; the Paeonia lactiflora extract is extracted from the entire plant of Paeonia lactiflora in a solvent mixture of water and glycerol in a weight ratio of 3: 7; and the nopal extract is extracted from the nopal cactus with the hot water. Fruit extraction.