TWI456199B - 生物檢測裝置及檢測方法 - Google Patents
生物檢測裝置及檢測方法 Download PDFInfo
- Publication number
- TWI456199B TWI456199B TW100149671A TW100149671A TWI456199B TW I456199 B TWI456199 B TW I456199B TW 100149671 A TW100149671 A TW 100149671A TW 100149671 A TW100149671 A TW 100149671A TW I456199 B TWI456199 B TW I456199B
- Authority
- TW
- Taiwan
- Prior art keywords
- liquid crystal
- electric field
- processing unit
- polymer film
- sample
- Prior art date
Links
- 238000001514 detection method Methods 0.000 title claims 7
- 238000000034 method Methods 0.000 title claims 2
- 230000005684 electric field Effects 0.000 claims 19
- 229920000106 Liquid crystal polymer Polymers 0.000 claims 11
- 239000004977 Liquid-crystal polymers (LCPs) Substances 0.000 claims 11
- 239000004973 liquid crystal related substance Substances 0.000 claims 8
- 239000000758 substrate Substances 0.000 claims 7
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims 6
- 239000007788 liquid Substances 0.000 claims 6
- 229920006254 polymer film Polymers 0.000 claims 5
- 230000004071 biological effect Effects 0.000 claims 4
- 229920000642 polymer Polymers 0.000 claims 4
- 235000012000 cholesterol Nutrition 0.000 claims 3
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 claims 3
- 230000002209 hydrophobic effect Effects 0.000 claims 2
- 239000004988 Nematic liquid crystal Substances 0.000 claims 1
- 238000004166 bioassay Methods 0.000 claims 1
- 239000000178 monomer Substances 0.000 claims 1
- 239000002861 polymer material Substances 0.000 claims 1
- 238000010998 test method Methods 0.000 claims 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
- B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
- B01L3/502792—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics for moving individual droplets on a plate, e.g. by locally altering surface tension
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
- B01L2400/0427—Electrowetting
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Dispersion Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Ecology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Claims (15)
- 一種生物檢測裝置，係用以檢測呈液狀之一檢體，該檢測裝置包含：一基板；一液晶聚合物薄膜，係包含一液晶分子及一高分子聚合物，且該液晶聚合物薄膜上放置該檢體；一電場單元，其係設置於該基板及該液晶聚合物薄膜之間；一電源供應器，係連結至該電場單元並提供一電壓形成一電場，利用該電場使該液晶分子轉向進而改變該液晶聚合物薄膜的親疏水性，以驅動該檢體移動；一處理單元，係連接於該電源供應器，以控制該電源供應器提供至該電場單元之該電壓；以及一影像感測器，係連結於該處理單元，並擷取該液晶聚合物薄膜表面上之該檢體因該電場變化所產生親疏水性移動之一影像資料，而提供至該處理單元；其中，該處理單元接收該影像資料，並據以分析該檢體之一生物特性；其中該處理單元係分析該影像資料中該檢體的接觸角、移動距離或崩塌速度之變化數據，並與一參考資料庫比對，進而判斷該檢體之生物特性。
- 如申請專利範圍第1項所述之生物檢測裝置，其中該電場單元包括複數個電極對，其中各該電極對併排設置於該基板上，各該電極對係包含： 一第一帶狀電極，其係包括複數個第一延伸部，各該第一延伸部係間隔排列；以及一第二帶狀電極，其係包括複數個第二延伸部，各該第二延伸部係間隔排列，且各該第二延伸部與各第一延伸部交替排列。
- 如申請專利範圍第1項所述之生物檢測裝置，其中該液晶分子係為正型之向列型液晶。
- 如申請專利範圍第1項所述之生物檢測裝置，其中該高分子聚合物之材料為有機或無機液態結晶單體。
- 如申請專利範圍第1項所述之生物檢測裝置，其中該液晶分子與該高分子聚合物之重量百分濃度比例介於5：5至8：2。
- 如申請專利範圍第1項所述之生物檢測裝置，其中該生物特性係為三酸甘油酯或高密度膽固醇之濃度。
- 如申請專利範圍第1項所述之生物檢測裝置，其中該影像感測器係為高速CCD攝影機。
- 一種生物檢測方法，係用以檢測呈液狀之一檢體，包含下列步驟：設置一液晶聚合物薄膜於一基板上；設置該檢體於該液晶聚合物薄膜上；設置一電場單元於該基板及該液晶聚合物薄膜之間；提供一電源供應器連結至該電場單元以提供一電場；藉由該電場使該液晶分子轉向進而改變該液晶聚合物薄膜的親疏水性，以驅動該檢體移動；設置一處理單元連接於該電源供應器以控制該電源供應器提供至該電場單元之該電壓；以及設置一影像感測器連結於該處理單元，並擷取該液晶聚合物薄膜 表面上之該檢體因該電場變化所產生親疏水性移動之一影像資料，而提供至該處理單元；其中，該處理單元接收該影像資料，並據以分析該檢體之生物特性；其中該處理單元係分析該影像資料中該檢體的接觸角、移動距離或崩塌速度之變化數據，並與一參考資料庫比對，進而判斷該檢體之生物特性。
- 如申請專利範圍第8項所述之生物檢測方法，其中更包含以5：5至8：2之重量百分濃度之比例範圍混合一液晶分子和一高分子聚合物，以形成該液晶聚合物薄膜之步驟。
- 如申請專利範圍第8項所述之生物檢測方法，其中該影像感測器係為高速CCD攝影機。
- 如申請專利範圍第8項所述之生物檢測方法，其中該生物特性係為三酸甘油酯或高密度膽固醇之濃度。
- 一種生物檢測方法，係用以檢測呈液狀且具有預設濃度之一液滴，包含下列步驟：提供一基板；設置一電場單元於該基板及一高分子薄膜之間；設置該液滴於未施加電壓之該高分子薄膜上；提供一電源供應器連結至該電場單元以提供一電場；藉由該電場使一液晶分子轉向進而改變該高分子薄膜的親疏水性，以驅動該液滴移動；設置一處理單元連接於該電源供應器以控制該電源供應器提供至該電場單元之該電壓；以及藉由一影像感測器連結於該處理單元，並擷取該高分子薄膜表面上之該液滴因該電場變化所產生親疏水性移動之一包含一接觸角 影像資料的影像資料，而提供至該處理單元；其中，該處理單元接收該影像資料，並據以分析該液滴之一生物特性；其中該處理單元係分析該影像資料中該液滴的接觸角、移動距離或崩塌速度之變化數據，並與一參考資料庫比對，進而判斷該檢體之生物特性。
- 如申請專利範圍第12項所述之生物檢測方法，其中更包含5：5至8：2之重量百分濃度之比例範圍混合該液晶分子和一高分子聚合物，以形成該高分子薄膜之步驟。
- 如申請專利範圍第12項所述之生物檢測方法，其中該影像感測器係為高速CCD攝影機。
- 如申請專利範圍第12項所述之生物檢測方法，其中該生物特性係為三酸甘油酯或高密度膽固醇之濃度。
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TW100149671A TWI456199B (zh) | 2011-12-29 | 2011-12-29 | 生物檢測裝置及檢測方法 |
| US13/595,648 US9164077B2 (en) | 2011-12-29 | 2012-08-27 | Biological detection device and method utilizing LCPCF film for testing liquid form samples containing triglyceride/HDL disposed thereon |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TW100149671A TWI456199B (zh) | 2011-12-29 | 2011-12-29 | 生物檢測裝置及檢測方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| TW201326818A TW201326818A (zh) | 2013-07-01 |
| TWI456199B true TWI456199B (zh) | 2014-10-11 |
Family
ID=48693758
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| TW100149671A TWI456199B (zh) | 2011-12-29 | 2011-12-29 | 生物檢測裝置及檢測方法 |
Country Status (2)
| Country | Link |
|---|---|
| US (1) | US9164077B2 (zh) |
| TW (1) | TWI456199B (zh) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101361072B1 (ko) * | 2012-12-17 | 2014-02-10 | 이상현 | 접촉면 확산 계수 기반 액체 내 지방 침투도 측정 방법 |
| US11165993B2 (en) | 2012-12-17 | 2021-11-02 | Femtobiomed Inc. | Contact area diffusion factor for quantifying fat contents of liquid |
| KR101872380B1 (ko) | 2014-01-30 | 2018-06-28 | 휴렛-팩커드 디벨롭먼트 컴퍼니, 엘.피. | 미세유체 감지 장치 |
| RU2657304C2 (ru) * | 2014-02-07 | 2018-06-13 | Фемтобиомед Инк. | Коэффициент диффузии площади контакта для количественного определения содержания жиров в жидкости |
| CN105107558A (zh) * | 2015-09-14 | 2015-12-02 | 安徽博微长安电子有限公司 | 一种全自动液滴生成系统及其控制方法 |
| CA2995732A1 (en) * | 2015-09-16 | 2017-03-23 | Thermo Electron Scientific Instruments Llc | Image analysis system and method |
| KR102086840B1 (ko) * | 2017-04-26 | 2020-03-09 | 주식회사 펨토바이오메드 | 접촉면 확산계수를 이용한 액체 내 소수성 성분의 정량 방법 및 이를 이용한 질환의 진단에 대한 정보 제공 방법 |
| JP2018136327A (ja) * | 2018-03-30 | 2018-08-30 | 株式会社フェムトバイオメド | 接触面拡散係数を用いた液体内の脂肪含量測定方法 |
| CN110390840A (zh) * | 2019-07-24 | 2019-10-29 | 安徽新知数媒信息科技有限公司 | 一种化学反应微距摄影装置及其工作方法 |
| CN115236001A (zh) * | 2022-07-26 | 2022-10-25 | 豪威半导体（上海）有限责任公司 | 图像传感器及其制作方法 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6911132B2 (en) * | 2002-09-24 | 2005-06-28 | Duke University | Apparatus for manipulating droplets by electrowetting-based techniques |
| TW200624802A (en) * | 2004-12-31 | 2006-07-16 | Ind Tech Res Inst | Droplet controlling apparatus, manufacturing method, controlling method and digital flow inspection apparatus |
| TW200842350A (en) * | 2007-04-26 | 2008-11-01 | Jing-Tang Yang | A novel method and control devices for changing wettability on solid surface by electric or magnetic field |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10002697A1 (de) | 2000-01-22 | 2001-08-02 | Daimler Chrysler Ag | Reversibel schaltbare Primer und Korrosionsschutz für Metalle |
| US7020355B2 (en) * | 2001-11-02 | 2006-03-28 | Massachusetts Institute Of Technology | Switchable surfaces |
| AU2002364609B2 (en) | 2001-12-28 | 2008-04-03 | Polymer Technology Systems, Inc. | Test strip for determining concentration of multiple analytes in a single fluid sample |
| DE60207196T2 (de) | 2002-04-09 | 2006-07-20 | Cholestech Corp., Hayward | Verfahren und Vorrichtung zur Quantifizierung von Lipoprotein-Cholesterol hoher Dichte |
| US7687239B2 (en) * | 2003-07-12 | 2010-03-30 | Accelrs Technology Corporation | Sensitive and rapid determination of antimicrobial susceptibility |
| TWI372783B (en) | 2005-04-27 | 2012-09-21 | Kyowa Medex Co Ltd | A process for measuring the cholesterol in high density lipoprotein |
| JP2009013164A (ja) | 2007-06-04 | 2009-01-22 | Hitachi High-Technologies Corp | 高密度リポ蛋白測定装置および分離方法 |
-
2011
- 2011-12-29 TW TW100149671A patent/TWI456199B/zh active
-
2012
- 2012-08-27 US US13/595,648 patent/US9164077B2/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6911132B2 (en) * | 2002-09-24 | 2005-06-28 | Duke University | Apparatus for manipulating droplets by electrowetting-based techniques |
| TW200624802A (en) * | 2004-12-31 | 2006-07-16 | Ind Tech Res Inst | Droplet controlling apparatus, manufacturing method, controlling method and digital flow inspection apparatus |
| TW200842350A (en) * | 2007-04-26 | 2008-11-01 | Jing-Tang Yang | A novel method and control devices for changing wettability on solid surface by electric or magnetic field |
Non-Patent Citations (2)
| Title |
|---|
| Ya-Ping Chiu et. al., "Electrically surface-driven switchable wettability of liquid crystal/polymer composite film"；Appl. Phys. Lett. 96, 131902 (March 2010) * |
| Yi-Hsin Lin et.al., "Electrically tunable wettability of liquid crystal/polymer composite films", Opt Express. 2008 Oct 27;16(22):17591-8 * |
Also Published As
| Publication number | Publication date |
|---|---|
| US9164077B2 (en) | 2015-10-20 |
| US20130167621A1 (en) | 2013-07-04 |
| TW201326818A (zh) | 2013-07-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| TWI456199B (zh) | 生物檢測裝置及檢測方法 | |
| JP7064007B2 (ja) | 薄膜トランジスタと容量感知とを伴う二重基板を含むデジタルマイクロ流体デバイス | |
| Viehrig et al. | Quantitative SERS assay on a single chip enabled by electrochemically assisted regeneration: a method for detection of melamine in milk | |
| He et al. | Thermophoretic manipulation of DNA translocation through nanopores | |
| Boussouar et al. | Single nanochannel platform for detecting chiral drugs | |
| Ghosh Dastider et al. | Low concentration E. coli O157: H7 bacteria sensing using microfluidic MEMS biosensor | |
| US20170030836A1 (en) | Spectroscopic sensor and method for manufacturing the same | |
| He et al. | Detection of trace microcystin-LR on a 20 MHz QCM sensor coated with in situ self-assembled MIPs | |
| Buss et al. | From micro to nano thin polymer layers: thickness and concentration dependence of sorption and the solvent diffusion coefficient | |
| Lin et al. | Dual pH gradient and voltage modulation of ion transport and current rectification in biomimetic nanopores functionalized with a pH-tunable polyelectrolyte | |
| Rath et al. | Chemiresistive sensor for enhanced CO2 gas monitoring | |
| Tsutsui et al. | Solid-state nanopore time-of-flight mass spectrometer | |
| Liu et al. | Selective single-bacterium analysis and motion tracking based on conductive bulk-surface imprinting | |
| Panda et al. | Characterizing the Janus colloidal particles in AC electric field and a step towards label-free cargo manipulation | |
| Si et al. | Navigated delivery of peptide to the nanopore using in-plane heterostructures of MoS2 and SnS2 for protein sequencing | |
| Liu et al. | Polarization-induced local pore-wall functionalization for biosensing: from micropore to nanopore | |
| Li et al. | Intelligent droplet tracking with correlation filters for digital microfluidics | |
| KR101646182B1 (ko) | 바이오 센서 | |
| Oikonomou et al. | Chemocapacitor performance modeling by means of polymer swelling optical measurements | |
| Kwak et al. | Size-selective particle trapping in dielectrophoretic corral traps | |
| Hu et al. | Dielectrophoresis: Measurement technologies and auxiliary sensing applications | |
| Wu et al. | Large-area high-contrast hydrophobic/hydrophilic patterned surface for robust electrowetting devices | |
| CN201188104Y (zh) | 高通量测试芯片 | |
| Hu et al. | Ratio of HbA1c to hemoglobin on ring-shaped interdigital electrode arrays based on impedance measurement | |
| Liu et al. | Conformation influence of DNA on the detection signal through solid-state nanopores |