SU73965A1 - Insulin Production Method - Google Patents

Description

Obtaining insulin is performed by pancreatic swelling in the pancreas with water acidified with MH; epa; ibHoii is acidic, followed by stepwise release of insulin from an aqueous solution of benzoic acid. Prod. process flow 2-2.5 hours Extraction is carried out without preheating in the ratio: 1 kg of the sub-pancreas, 2 liters of water. 20 ml to 1 cent) of sulfuric acid: acid.

This is followed by the separation of the treated gland from the aqueous extract, through the bags. The treatment of the iron by removing the extract from it is wrung out on the press. The resulting nrn extract is added to the bulk of the extract obtained at, i.a.

Then the extract is distilled by the precipitation of ammonium sulphate with ammonium ... The mother liquor from the efflorescence is again subjected to the unvented exudate | Vysol is dissolved in 80% alcohol, which is mixed with 5 yurma. |) .iCTBcpo:; salt). Filtration solution.

Fn .: L7rat;: Drops down to the precipitation of 10 volume. :: 96-i; oro ethyl sprint: first precipitated 1e is carried out by one volume of alcohol; at the same time, in the precipitate ba, the powerful substances are separated by filtration, and then the filtrate is precipitated by 9 more volumes (but relative to the initial amount of filtrate).

From raw material, a 10% solution is prepared, a 1P solution, from which the beanbir — a mixture of benzoic acid. In this case, a pM of 4.8 is set in the solution, in which the neutron absorption is maximum insch. an amount of a saturated solution of benzoic acid is added to the water solution; benzoic kns :: oto are separated from nnslin by treatment with 96% ethyl stripping.

The resulting semi-refined syrye inicy.iHua: o,. Vsr pets 01 onchat.) K; oG; cleaning the usual method.

Subject invention

 A method of producing insulin by removing it from the podzhepudochkoy gland with iomon and mineral acid, staged salting out of the salt filtrate and absorbing insulin from an aqueous solution of benzoic acid, characterized in that the extraction is carried out at normal temperature, without heating, at pH from 1.4 p .0 2, for 2-2.5 hours. For this purpose, in order to facilitate filtration, the pH is adjusted from 6 to 8 by adding alkali and immediately, without a filter, is adjusted to a pH of 2-3.

2. The output according to claim 1, that is, that the deposition of bU-60 ° desalted vysol solutions is performed first by a single volume of 96-epypt and after the separation of the remaining ballast ones: 96 volumes of alcohol.

3. Wavelengths but also. 1, characterized in that the semi-finished product obtained in steps 1 and 2 is subjected to purification by absorption with benzoic acid. In an acidic aqueous solution of semi-apricate, the pH is adjusted to 4.8 and a saturated alcohol solution of benzoic acid is added. Separation of iisulin from benzoic acid is () fixed with alcohol.