RU2018133691A - Транспозонная система и способы ее применения - Google Patents

Транспозонная система и способы ее применения Download PDF

Info

Publication number: RU2018133691A
Authority: RU; Russia
Prior art keywords: sequence encoding; dna sequence; transposon; paragraphs; electroporation
Prior art date: 2016-02-26

Application number

RU2018133691A

Other languages

English (en)

Russian (ru)

Other versions

RU2018133691A3 (es

Inventor

Девон ШЕДЛОК

Дэвид ХЕРМАНСОН

Эрик ОСТЕРТАГ

Original Assignee

Посейда Терапьютикс, Инк.

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2016-02-26

Filing date

2017-02-24

Publication date

2020-03-26

2017-02-24 Application filed by Посейда Терапьютикс, Инк. filed Critical Посейда Терапьютикс, Инк.

2020-03-26 Publication of RU2018133691A publication Critical patent/RU2018133691A/ru

2020-07-29 Publication of RU2018133691A3 publication Critical patent/RU2018133691A3/ru

Links

108091028043 Nucleic acid sequence Proteins 0.000 claims 106
238000000034 method Methods 0.000 claims 90
102000008579 Transposases Human genes 0.000 claims 62
108010020764 Transposases Proteins 0.000 claims 62
238000004520 electroporation Methods 0.000 claims 38
210000002865 immune cell Anatomy 0.000 claims 33
150000007523 nucleic acids Chemical group 0.000 claims 19
238000006243 chemical reaction Methods 0.000 claims 18
239000003153 chemical reaction reagent Substances 0.000 claims 18
240000007019 Oxalis corniculata Species 0.000 claims 11
108091033409 CRISPR Proteins 0.000 claims 8
125000003275 alpha amino acid group Chemical group 0.000 claims 6
210000004405 cytokine-induced killer cell Anatomy 0.000 claims 6
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims 6
210000000581 natural killer T-cell Anatomy 0.000 claims 6
210000000822 natural killer cell Anatomy 0.000 claims 6
239000001963 growth medium Substances 0.000 claims 5
108090000695 Cytokines Proteins 0.000 claims 4
102000004127 Cytokines Human genes 0.000 claims 4
108010042407 Endonucleases Proteins 0.000 claims 3
102000004533 Endonucleases Human genes 0.000 claims 3
210000001744 T-lymphocyte Anatomy 0.000 claims 3
201000010099 disease Diseases 0.000 claims 3
208000035475 disorder Diseases 0.000 claims 3
230000004936 stimulating effect Effects 0.000 claims 3
108020004638 Circular DNA Proteins 0.000 claims 2
108020004414 DNA Proteins 0.000 claims 2
108090000172 Interleukin-15 Proteins 0.000 claims 2
108010002350 Interleukin-2 Proteins 0.000 claims 2
108010002586 Interleukin-7 Proteins 0.000 claims 2
108010003723 Single-Domain Antibodies Proteins 0.000 claims 2
239000000427 antigen Substances 0.000 claims 2
230000000890 antigenic effect Effects 0.000 claims 2
102000036639 antigens Human genes 0.000 claims 2
108091007433 antigens Proteins 0.000 claims 2
239000012634 fragment Substances 0.000 claims 2
238000010362 genome editing Methods 0.000 claims 2
238000000338 in vitro Methods 0.000 claims 2
108010074108 interleukin-21 Proteins 0.000 claims 2
108020004999 messenger RNA Proteins 0.000 claims 2
239000013600 plasmid vector Substances 0.000 claims 2
238000003752 polymerase chain reaction Methods 0.000 claims 2
108090000623 proteins and genes Proteins 0.000 claims 2
102000004169 proteins and genes Human genes 0.000 claims 2
102000005962 receptors Human genes 0.000 claims 2
239000013598 vector Substances 0.000 claims 2
108091032973 (ribonucleotides)n+m Proteins 0.000 claims 1
FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 1
241000283690 Bos taurus Species 0.000 claims 1
241000282832 Camelidae Species 0.000 claims 1
241000282836 Camelus dromedarius Species 0.000 claims 1
241000282472 Canis lupus familiaris Species 0.000 claims 1
108091026890 Coding region Proteins 0.000 claims 1
208000035473 Communicable disease Diseases 0.000 claims 1
102220605874 Cytosolic arginine sensor for mTORC1 subunit 2_D10A_mutation Human genes 0.000 claims 1
108700022150 Designed Ankyrin Repeat Proteins Proteins 0.000 claims 1
102000004190 Enzymes Human genes 0.000 claims 1
108090000790 Enzymes Proteins 0.000 claims 1
241000283086 Equidae Species 0.000 claims 1
108091029865 Exogenous DNA Proteins 0.000 claims 1
241000282326 Felis catus Species 0.000 claims 1
108010021625 Immunoglobulin Fragments Proteins 0.000 claims 1
102000008394 Immunoglobulin Fragments Human genes 0.000 claims 1
108091007491 NSP3 Papain-like protease domains Proteins 0.000 claims 1
206010028980 Neoplasm Diseases 0.000 claims 1
241000283973 Oryctolagus cuniculus Species 0.000 claims 1
241000288906 Primates Species 0.000 claims 1
241000283984 Rodentia Species 0.000 claims 1
241000282887 Suidae Species 0.000 claims 1
108091008108 affimer Proteins 0.000 claims 1
230000000735 allogeneic effect Effects 0.000 claims 1
201000011510 cancer Diseases 0.000 claims 1
230000003197 catalytic effect Effects 0.000 claims 1
238000003776 cleavage reaction Methods 0.000 claims 1
238000012239 gene modification Methods 0.000 claims 1
230000005017 genetic modification Effects 0.000 claims 1
235000013617 genetically modified food Nutrition 0.000 claims 1
208000015181 infectious disease Diseases 0.000 claims 1
108090000765 processed proteins & peptides Proteins 0.000 claims 1
108091008146 restriction endonucleases Proteins 0.000 claims 1
230000007017 scission Effects 0.000 claims 1
230000035899 viability Effects 0.000 claims 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0636—T lymphocytes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/90—Vectors containing a transposable element
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Genetics & Genomics (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Biomedical Technology (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Zoology (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Microbiology (AREA)
Immunology (AREA)
Molecular Biology (AREA)
Medicinal Chemistry (AREA)
Cell Biology (AREA)
Hematology (AREA)
Plant Pathology (AREA)
Biophysics (AREA)
Physics & Mathematics (AREA)
Veterinary Medicine (AREA)
Pharmacology & Pharmacy (AREA)
Animal Behavior & Ethology (AREA)
Public Health (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
General Chemical & Material Sciences (AREA)
Chemical Kinetics & Catalysis (AREA)
Oncology (AREA)
Communicable Diseases (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Enzymes And Modification Thereof (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Peptides Or Proteins (AREA)
Medicinal Preparation (AREA)

RU2018133691A 2016-02-26 2017-02-24 Транспозонная система и способы ее применения RU2018133691A (ru)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
US201662300387P	2016-02-26	2016-02-26
US62/300,387		2016-02-26
PCT/US2017/019531 WO2017147538A1 (en)	2016-02-26	2017-02-24	Transposon system and methods of use

Publications (2)

Publication Number	Publication Date
RU2018133691A true RU2018133691A (ru)	2020-03-26
RU2018133691A3 RU2018133691A3 (es)	2020-07-29

Family

ID=58314517

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
RU2018133691A RU2018133691A (ru)	2016-02-26	2017-02-24	Транспозонная система и способы ее применения

Country Status (12)

Country	Link
EP (1)	EP3420090A1 (es)
JP (1)	JP2019506177A (es)
KR (1)	KR20180131545A (es)
CN (1)	CN109312361A (es)
AU (1)	AU2017224111A1 (es)
BR (1)	BR112018067536A2 (es)
CA (1)	CA3015642A1 (es)
IL (1)	IL261343A (es)
MX (1)	MX2018010288A (es)
RU (1)	RU2018133691A (es)
SG (1)	SG11201807134RA (es)
WO (1)	WO2017147538A1 (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EP3494132A4 (en)	2016-08-03	2020-03-18	Washington University	Gene editing of car-t cells for the treatment of t cell malignancies with chimeric antigen receptors
US20190119636A1 (en) *	2017-10-23	2019-04-25	Poseida Therapeutics, Inc.	Modified stem cell memory t cells, methods of making and methods of using same
EP3555273B1 (en)	2016-12-16	2024-05-22	B-Mogen Biotechnologies, Inc.	ENHANCED hAT FAMILY TRANSPOSON-MEDIATED GENE TRANSFER AND ASSOCIATED COMPOSITIONS, SYSTEMS, AND METHODS
US11278570B2 (en)	2016-12-16	2022-03-22	B-Mogen Biotechnologies, Inc.	Enhanced hAT family transposon-mediated gene transfer and associated compositions, systems, and methods
CN108728477B (zh) *	2017-04-24	2022-02-22	华东理工大学	一种高效的转座突变系统及构建方法
US20210115453A1 (en) *	2017-08-31	2021-04-22	Poseida Therapeutics, Inc.	Transposon system and methods of use
AU2018329741B2 (en) *	2017-09-08	2025-02-20	Poseida Therapeutics, Inc.	Compositions and methods for chimeric ligand receptor (CLR)-mediated conditional gene expression
US10329543B2 (en)	2017-10-23	2019-06-25	Poseida Therapeutics, Inc.	Modified stem cell memory T cells, methods of making and methods of using same
CN112543808A (zh)	2018-06-21	2021-03-23	比莫根生物科技公司	增强的hAT家族转座子介导的基因转移及相关组合物、系统和方法
US20220170044A1 (en) *	2019-02-08	2022-06-02	Dna Twopointo, Inc.	Transposon-based modifications of immune cells
CN111926041B (zh) *	2020-10-16	2020-12-25	广州吉妮欧生物科技有限公司	一种SARS-CoV-2假病毒小鼠体内包装系统及其制备方法
CN114246986B (zh) *	2021-12-29	2022-08-23	中国人民解放军陆军军医大学	一种基于原位调控免疫反应的心血管植入物及其制备方法
CN118185901B (zh) *	2022-12-12	2025-03-21	北京精缮生物科技有限责任公司	一种PBase蛋白、融合蛋白、核酸和基因整合系统及应用
CN116590341A (zh) *	2023-07-13	2023-08-15	山东维真生物科技有限公司	一种腺病毒-PiggyBac系统及其制备方法和应用

Family Cites Families (6)

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US4554101A (en)	1981-01-09	1985-11-19	New York Blood Center, Inc.	Identification and preparation of epitopes on antigens and allergens on the basis of hydrophilicity
EP2160461B1 (en)	2007-07-04	2012-08-08	Max-Delbrück-Centrum für Molekulare Medizin (MDC)	Hyperactive variants of the transposase protein of the transposon system sleeping beauty
EP2401376A4 (en) *	2009-02-25	2012-11-28	Univ Johns Hopkins	TRANSPOSON PIGGYBAC VARIANTS AND METHODS OF USE
EP2401367B1 (en) *	2009-02-26	2016-11-30	Transposagen Biopharmaceuticals, Inc.	Hyperactive piggybac transposases
US20130045491A1 (en) *	2011-07-19	2013-02-21	Derya Unutmaz	Methods for activating t cells
US20150322439A1 (en) *	2012-11-19	2015-11-12	Nature Technology Corporation	Replicative minicircle vectors with improved expression

2017
- 2017-02-24 BR BR112018067536A patent/BR112018067536A2/pt not_active IP Right Cessation
- 2017-02-24 CN CN201780025816.1A patent/CN109312361A/zh active Pending
- 2017-02-24 JP JP2018544891A patent/JP2019506177A/ja active Pending
- 2017-02-24 RU RU2018133691A patent/RU2018133691A/ru not_active Application Discontinuation
- 2017-02-24 EP EP17711046.7A patent/EP3420090A1/en not_active Withdrawn
- 2017-02-24 AU AU2017224111A patent/AU2017224111A1/en not_active Abandoned
- 2017-02-24 KR KR1020187027345A patent/KR20180131545A/ko not_active Withdrawn
- 2017-02-24 MX MX2018010288A patent/MX2018010288A/es unknown
- 2017-02-24 SG SG11201807134RA patent/SG11201807134RA/en unknown
- 2017-02-24 WO PCT/US2017/019531 patent/WO2017147538A1/en not_active Ceased
- 2017-02-24 CA CA3015642A patent/CA3015642A1/en not_active Abandoned
2018
- 2018-08-23 IL IL261343A patent/IL261343A/en unknown

Also Published As

Publication number	Publication date
SG11201807134RA (en)	2018-09-27
CN109312361A (zh)	2019-02-05
JP2019506177A (ja)	2019-03-07
CA3015642A1 (en)	2017-08-31
EP3420090A1 (en)	2019-01-02
MX2018010288A (es)	2019-06-06
KR20180131545A (ko)	2018-12-10
RU2018133691A3 (es)	2020-07-29
IL261343A (en)	2018-10-31
BR112018067536A2 (pt)	2019-02-05
AU2017224111A1 (en)	2018-09-13
WO2017147538A1 (en)	2017-08-31

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Legal Events

Date	Code	Title	Description
2021-07-10	FA92	Acknowledgement of application withdrawn (lack of supplementary materials submitted)	Effective date: 20210702