[go: up one dir, main page]

RU2018125950A - Усиленная ферментация - Google Patents

Усиленная ферментация Download PDF

Info

Publication number
RU2018125950A
RU2018125950A RU2018125950A RU2018125950A RU2018125950A RU 2018125950 A RU2018125950 A RU 2018125950A RU 2018125950 A RU2018125950 A RU 2018125950A RU 2018125950 A RU2018125950 A RU 2018125950A RU 2018125950 A RU2018125950 A RU 2018125950A
Authority
RU
Russia
Prior art keywords
catalase
fragment
polypeptide
microorganism
seq
Prior art date
Application number
RU2018125950A
Other languages
English (en)
Inventor
Макс Фабиан ФЕЛЛЕ
Стефан ЕНЕВАЙН
Хандтке СТЕФАН
Биргит ФОЙГТ
Томас ШВЕДЕР
Михаэль ХЕККЕР
Original Assignee
Басф Се
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Басф Се filed Critical Басф Се
Publication of RU2018125950A publication Critical patent/RU2018125950A/ru

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0065Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6402Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals
    • C12N9/6405Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals not being snakes
    • C12N9/6408Serine endopeptidases (3.4.21)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477Hemicellulases not provided in a preceding group
    • C12N9/248Xylanases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477Hemicellulases not provided in a preceding group
    • C12N9/2488Mannanases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y111/00Oxidoreductases acting on a peroxide as acceptor (1.11)
    • C12Y111/01Peroxidases (1.11.1)
    • C12Y111/01006Catalase (1.11.1.6)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21Serine endopeptidases (3.4.21)
    • C12Y304/21062Subtilisin (3.4.21.62)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Detergent Compositions (AREA)

Claims (21)

1. Способ защиты микробиологически полученного вещества от окисления, включающий
a) трансформацию микроорганизма, способного продуцировать указанное вещество, рекомбинантной нуклеиновой кислотой, содержащей ген каталазы, кодирующий полипептид, функционально связанный с одной или более контрольными последовательностями, которые управляют продуцированием полипептида в хозяине экспрессии, где полипептид представляет собой
i) каталазу, имеющую по меньшей мере 60% идентичность аминокислотной последовательности с SEQ ID NO. 1, или
ii) фрагмент каталазы согласно i), где фрагмент имеет каталазную активность,
или
обеспечение микроорганизма, содержащего в качестве гетерологичного гена, ген каталазы, кодирующий
i) каталазу, имеющую по меньшей мере 60% идентичность аминокислотной последовательности с SEQ ID NO. 1, или
ii) фрагмент каталазы согласно i), где фрагмент имеет каталазную активность,
b) культивирование микроорганизма при условиях, позволяющих экспрессию указанного гена каталазы.
2. Способ ферментации для получения продукта ферментации, включающий стадии
a) трансформации микроорганизма, способного продуцировать указанное вещество, рекомбинантной нуклеиновой кислотой, содержащей ген каталазы, кодирующий полипептид, функционально связанный с одной или более контрольными последовательностями, которые управляют продуцированием полипептида в хозяине экспрессии, где полипептид представляет собой
i) каталазу, имеющую по меньшей мере 60% идентичность аминокислотной последовательности с SEQ ID NO. 1, или
ii) фрагмент каталазы согласно i), где фрагмент имеет каталазную активность, или '
обеспечения микроорганизма, содержащего в качестве гетерологичного гена, ген каталазы, кодирующий
i) каталазу, имеющую по меньшей мере 60% идентичность аминокислотной последовательности с SEQ ID NO. 1, или
ii) фрагмент каталазы согласно i), где фрагмент имеет каталазную активность, и
b) культивирования микроорганизма при условиях, позволяющих экспрессию указанного гена каталазы и также позволяющих продуцирование указанного продукта ферментации.
3. Способ по п. 1 или 2, где полипептид содержит замещение, делецию и/или вставку в одном или более положениях.
4. Способ по п. 1 или 2, где контрольная последовательность представляет собой или содержит индуцируемый промотор, предпочтительно индуцируемый пероксидом водорода промотор.
5. Способ по п. 1 или 2, где нуклеиновая кислота представляет собой конструкцию нуклеиновой кислоты или экспрессионный вектор.
6. Способ по п. 1 или 2, где микробиологически получаемое вещество или продукт ферментации представляет собой белок, предпочтительно фермент, и предпочтительно выбрано из группы, состоящей из протеазы, амилазы, карбогидразы, липазы, целлюлазы, пуллуланазы, кутиназы, пектиназы, маннаназы, арабиназы, галактаназы, ксиланазы, оксидазы, например лакказы, пероксидазы, изомеразы, трансферазы, киназы и фосфатазы, где предпочтительными протеазами являются субтилизин протеаз.
RU2018125950A 2015-12-14 2016-12-08 Усиленная ферментация RU2018125950A (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP15199815 2015-12-14
EP15199815.0 2015-12-14
PCT/EP2016/080171 WO2017102524A1 (en) 2015-12-14 2016-12-08 Enhanced fermentation

Publications (1)

Publication Number Publication Date
RU2018125950A true RU2018125950A (ru) 2020-01-16

Family

ID=55068759

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2018125950A RU2018125950A (ru) 2015-12-14 2016-12-08 Усиленная ферментация

Country Status (11)

Country Link
US (1) US10676728B2 (ru)
EP (1) EP3390621B1 (ru)
JP (1) JP2018537108A (ru)
KR (1) KR20180086210A (ru)
CN (1) CN108463549A (ru)
BR (1) BR112018011893A2 (ru)
CA (1) CA3007307A1 (ru)
DK (1) DK3390621T3 (ru)
MX (1) MX382752B (ru)
RU (1) RU2018125950A (ru)
WO (1) WO2017102524A1 (ru)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107557438A (zh) * 2017-10-12 2018-01-09 江苏省渔业技术推广中心 一种基于高通量测序检测青虾肠道乳酸菌的方法
CN115369064B (zh) * 2022-09-30 2023-07-07 海南师范大学 一种增强生防贝莱斯芽孢杆菌根际定殖和防效的多功能复合菌剂的开发和应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7405081B2 (en) * 2001-02-05 2008-07-29 Pan Shen Q Methods and kits for identifying scavengers of reactive oxygen species (ROS)
KR20040037009A (ko) * 2002-10-25 2004-05-04 (주) 피엘바이오 과산화수소수 분해 능력과 저항성이 이 뛰어난 신규미생물과 이들이 생산하는 카타라아제
JP2005015415A (ja) * 2003-06-27 2005-01-20 Torisutaa:Kk 毛髪用化粧料組成物及びその製造方法
WO2010074972A1 (en) 2008-12-15 2010-07-01 Novozymes, Inc. Polypeptides having catalase activity and polynucleotides encoding same
CN101525601A (zh) * 2009-04-17 2009-09-09 江南大学 一种添加乙醇和/或过氧化氢胁迫枯草芽孢杆菌发酵产过氧化氢酶的方法
US9637725B2 (en) * 2011-12-19 2017-05-02 Novozymes Inc. Polypeptides having catalase activity and polynucleotides encoding same

Also Published As

Publication number Publication date
KR20180086210A (ko) 2018-07-30
EP3390621A1 (en) 2018-10-24
US10676728B2 (en) 2020-06-09
JP2018537108A (ja) 2018-12-20
CN108463549A (zh) 2018-08-28
MX2018007327A (es) 2018-08-24
US20180371444A1 (en) 2018-12-27
CA3007307A1 (en) 2017-06-22
BR112018011893A2 (pt) 2018-12-04
EP3390621B1 (en) 2021-02-17
DK3390621T3 (da) 2021-05-10
MX382752B (es) 2025-03-13
WO2017102524A1 (en) 2017-06-22

Similar Documents

Publication Publication Date Title
ES2257774T3 (es) Hongos filamentosos carentes de proteasa alcalina.
CN101084302B (zh) 重组微生物
CN103898080B (zh) 具有增加产率的支链淀粉酶变体
CA2345356A1 (en) Transformation system in the field of filamentous fungal hosts
CN107574177B (zh) 用于改善的蛋白质分泌的表达载体
JP7376505B2 (ja) 粘性が低下した表現型を含む糸状菌株
JP2019528797A5 (ru)
CN104471066B (zh) 表达方法
CN101970658A (zh) 耐热性过氧化氢酶
RU2011145540A (ru) Новая протеаза грибов и ее применение
JP6993339B2 (ja) タンパク質産生の強化およびその方法
US20210102231A1 (en) Methods for controlling protease production
CN104630174A (zh) 磷脂酶c突变体及其用途
RU2010109432A (ru) Каппа-каррагиназа и композиции, содержащие каппа-каррагиназу
RU2013146665A (ru) Фермент протеаза и его применения
RU2018125950A (ru) Усиленная ферментация
RU2642324C2 (ru) Способ экспрессии
JP7799621B2 (ja) 糸状菌細胞におけるタンパク質産生の増強のための組成物及び方法
CN101978056B (zh) 质粒载体
JP2018514231A (ja) 増殖とタンパク質生成との分離
CN107109424A (zh) 真菌宿主菌株、dna构建体及使用方法
CN120731271A (zh) 多拷贝宿主细胞的生成
JP4496000B2 (ja) 宿主微生物
JP2021532775A (ja) タンパク質生産性が強化された表現型を含む突然変異及び遺伝子改変糸状菌株並びにそれらの方法
EP2173873A2 (en) Protein and dna sequence encoding a cold adapted subtilisin-like activity

Legal Events

Date Code Title Description
FA93 Acknowledgement of application withdrawn (no request for examination)

Effective date: 20191209