RU2017112973A - Синтетический двунаправленный промотор растения - Google Patents
Синтетический двунаправленный промотор растения Download PDFInfo
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- RU2017112973A RU2017112973A RU2017112973A RU2017112973A RU2017112973A RU 2017112973 A RU2017112973 A RU 2017112973A RU 2017112973 A RU2017112973 A RU 2017112973A RU 2017112973 A RU2017112973 A RU 2017112973A RU 2017112973 A RU2017112973 A RU 2017112973A
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- polynucleotide
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- 230000002457 bidirectional effect Effects 0.000 title claims 12
- 108091033319 polynucleotide Proteins 0.000 claims 28
- 239000002157 polynucleotide Substances 0.000 claims 28
- 102000040430 polynucleotide Human genes 0.000 claims 28
- 241000196324 Embryophyta Species 0.000 claims 14
- 241000209094 Oryza Species 0.000 claims 14
- 235000007164 Oryza sativa Nutrition 0.000 claims 13
- 108090000848 Ubiquitin Proteins 0.000 claims 13
- 102000044159 Ubiquitin Human genes 0.000 claims 13
- 235000009566 rice Nutrition 0.000 claims 13
- 230000009261 transgenic effect Effects 0.000 claims 11
- 238000000034 method Methods 0.000 claims 9
- 230000002363 herbicidal effect Effects 0.000 claims 3
- 230000014509 gene expression Effects 0.000 claims 2
- 239000004009 herbicide Substances 0.000 claims 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 1
- 241000219194 Arabidopsis Species 0.000 claims 1
- 240000000385 Brassica napus var. napus Species 0.000 claims 1
- 101100442689 Caenorhabditis elegans hdl-1 gene Proteins 0.000 claims 1
- 108091026890 Coding region Proteins 0.000 claims 1
- 230000004568 DNA-binding Effects 0.000 claims 1
- 244000068988 Glycine max Species 0.000 claims 1
- 240000002024 Gossypium herbaceum Species 0.000 claims 1
- 235000004341 Gossypium herbaceum Nutrition 0.000 claims 1
- 244000061176 Nicotiana tabacum Species 0.000 claims 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims 1
- 244000098338 Triticum aestivum Species 0.000 claims 1
- 240000008042 Zea mays Species 0.000 claims 1
- 230000000749 insecticidal effect Effects 0.000 claims 1
- 239000003550 marker Substances 0.000 claims 1
- 230000014075 nitrogen utilization Effects 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 235000016709 nutrition Nutrition 0.000 claims 1
- 230000035897 transcription Effects 0.000 claims 1
- 238000013518 transcription Methods 0.000 claims 1
- 230000001131 transforming effect Effects 0.000 claims 1
- 238000011144 upstream manufacturing Methods 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 1
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- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
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Claims (24)
1. Синтетический двунаправленный полинуклеотидный промотор убиквитина 3 риса, который содержит:
a) минимальный коровый промотор, который содержит полинуклеотидную последовательность с по меньшей мере 90% идентичностью последовательности с SEQ ID NO: 2;
b) интрон, который содержит полинуклеотидную последовательность с по меньшей мере 90% идентичностью последовательности с SEQ ID NO: 4 или SEQ ID NO: 6;
c) 5ʹ UTR, который содержит полинуклеотидную последовательность с по меньшей мере 90% идентичностью последовательности с SEQ ID NO: 3 или SEQ ID NO: 5;
d) промоторный элемент выше по направлению считывания, который содержит полинуклеотидную последовательность с по меньшей мере 90% идентичностью последовательностей с SEQ ID NO: 7 или SEQ ID NO: 8.
2. Синтетический двунаправленный полинуклеотидный промотор убиквитина 3 риса по п. 1, отличающийся тем, что синтетический двунаправленный полинуклеотидный промотор содержит полинуклеотидную последовательность с по меньшей мере 90% идентичностью последовательности с SEQ ID NO: 1.
3. Синтетический двунаправленный полинуклеотидный промотор убиквитина 3 риса по п. 2, который содержит первую полинуклеотидную последовательность, представляющую интерес, функционально связанную с 3ʹ-концом последовательности синтетического двунаправленного полинуклеотида.
4. Синтетический двунаправленный полинуклеотидный промотор убиквитина 3 риса по п. 3, который содержит вторую полинуклеотидную последовательность, представляющую интерес, функционально связанную с 5ʹ-концом последовательности синтетического двунаправленного полинуклеотида.
5. Синтетический двунаправленный полинуклеотидный промотор убиквитина 3 риса по п. 4, в котором полинуклеотидная последовательность, представляющая интерес, содержит определенный признак.
6. Синтетический двунаправленный полинуклеотидный промотор убиквитина 3 риса по п. 5, где признак выбирают из группы, состоящей из признака инсектицидной устойчивости, признака гербицидной устойчивости, признака эффективности использования азота, признака эффективности использования воды, признака алиментарного качества, признака связывания ДНК, признака селективного маркера и какого-либо их сочетания.
7. Способ получения трансгенной клетки растения, способ включает:
a) трансформацию клетки растения с использованием кассеты для экспрессии гена, содержащей синтетический двунаправленный полинуклеотидный промотор убиквитина 3 риса, функционально связанный с по меньшей мере одной полинуклеотидной последовательностью, представляющей интерес;
b) выделение трансформированной клетки растения, которая содержит кассету для экспрессии гена; и
c) получение трансгенной клетки растения, которая содержит синтетический двунаправленный полинуклеотидный промотор убиквитина 3 риса, функционально связанный по меньшей мере с одной полинуклеотидной последовательностью, представляющей интерес.
8. Способ по п. 7, в котором нуклеотидную последовательность, представляющую интерес, стабильно встраивают в геном трансгенной клетки растения.
9. Способ по п. 8, в котором трансгенная клетка растения представляет собой трансгенную клетку односеменодольного растения или трансгенную клетку двусеменодольного растения.
10. Способ по п. 9, в котором трансгенную клетку двусеменодольного растения выбирают из группы, состоящей из клетки растения Arabidopsis, клетки растения табака, клетки растения соевых бобов, клетки растения канолы и клетки растения хлопка.
11. Способ по п. 9, в котором трансгенную клетку односеменодольного растения выбирают из группы, состоящей из клетки растения маиса, клетки растения риса, клетки растения брахиподии и клетки растения пшеницы.
12. Способ по п. 7, в котором синтетический двунаправленный полинуклеотид убиквитина 3 риса содержит SEQ ID NO: 1.
13. Способ по п. 7, включающий первую полинуклеотидную последовательность, представляющую интерес, функционально связанную с 3ʹ-концом синтетического двунаправленного полинуклеотидного промотора убиквитина 3 риса, содержащего SEQ ID NO: 1.
14. Способ по п. 7, включающий вторую полинуклеотидную последовательность, представляющую интерес, функционально связанную с 5ʹ-концом синтетического двунаправленного полинуклеотидного промотора убиквитина 3 риса, содержащего SEQ ID NO: 1.
15. Трансгенная клетка растения, которая содержит синтетический двунаправленный полинуклеотидный промотор убиквитина 3 риса по п. 1.
16. Трансгенная клетка растения по п. 15, где двунаправленный полинуклеотидный промотор убиквитина 3 риса управляет транскрипцией признака устойчивости к гербициду.
17. Трансгенная клетка растения по п. 16, где признак устойчивости к гербициду содержит кодирующую последовательность aad-1.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462078214P | 2014-11-11 | 2014-11-11 | |
| US62/078,214 | 2014-11-11 | ||
| PCT/US2015/060134 WO2016077449A1 (en) | 2014-11-11 | 2015-11-11 | Synthetic bi-directional plant promoter |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2017112973A true RU2017112973A (ru) | 2018-12-14 |
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| RU2017112973A RU2017112973A (ru) | 2014-11-11 | 2015-11-11 | Синтетический двунаправленный промотор растения |
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| US (1) | US20190002905A9 (ru) |
| EP (1) | EP3218501A4 (ru) |
| JP (1) | JP2017532963A (ru) |
| KR (1) | KR20170081168A (ru) |
| CN (1) | CN106852156A (ru) |
| AR (1) | AR102612A1 (ru) |
| AU (1) | AU2015346442B2 (ru) |
| BR (1) | BR112017007446A2 (ru) |
| CA (1) | CA2964498A1 (ru) |
| CL (1) | CL2017000922A1 (ru) |
| CO (1) | CO2017003326A2 (ru) |
| IL (1) | IL251704A0 (ru) |
| MX (1) | MX2017004727A (ru) |
| PH (1) | PH12017500698A1 (ru) |
| RU (1) | RU2017112973A (ru) |
| TW (1) | TW201617451A (ru) |
| WO (1) | WO2016077449A1 (ru) |
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| TW201805425A (zh) * | 2016-06-16 | 2018-02-16 | 艾格里遺傳學股份有限公司 | 用於轉殖基因表現之植物啟動子與３’ｕｔｒ |
| CN118813634A (zh) * | 2017-01-19 | 2024-10-22 | 孟山都技术公司 | 植物调控元件及其用途 |
| AU2020427583A1 (en) * | 2020-02-05 | 2022-08-25 | Bioceres Llc | Wheat transgenic event IND-ØØ412-7 |
| CN117417934B (zh) * | 2023-10-27 | 2024-09-13 | 江南大学 | 跨菌种启动子、多菌种穿梭质粒及其应用 |
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|---|---|---|---|---|
| US8022271B2 (en) * | 2003-05-16 | 2011-09-20 | North Carolina State University | Polyubiquitin Rubi3 promoter and 5' regulatory sequences |
| US7989676B2 (en) * | 2006-08-31 | 2011-08-02 | Ceres, Inc. | Nucleotide sequences and corresponding polypeptides conferring modulated plant characteristics |
| US8344211B2 (en) * | 2008-08-13 | 2013-01-01 | Ceres, Inc. | Plant nucleotide sequences and corresponding polypeptides |
| UA117654C2 (uk) * | 2009-11-06 | 2018-09-10 | Агрівіда, Інк. | Трансгенна рослина, яка експресує фермент, що деградує клітинну стінку, і вектор експресії |
| BR112013023003B1 (pt) * | 2011-03-07 | 2020-12-15 | Agrivida, Inc | Método para produção de açúcares solúveis a partir de material de planta genéticamente modificada e cassete de expressão |
| EP2797407A4 (en) * | 2011-12-30 | 2015-07-08 | Dow Agrosciences Llc | METHOD AND CONSTRUCTION FOR SYNTHETIC BIDIRECTIONAL SCBV PLANT PROMOTER |
| HK1201121A1 (en) * | 2011-12-30 | 2015-08-28 | Dow Agrosciences Llc | Construct and method for synthetic bidirectional plant promoter ubi1 |
| UA119135C2 (uk) * | 2012-09-07 | 2019-05-10 | ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі | Спосіб отримання трансгенної рослини |
| BR112015011152A2 (pt) * | 2012-11-14 | 2017-08-29 | Agrivida Inc | Planta geneticamente modificada, construção genética e método de processamento agrícola ou preparação de alimentação para animais |
-
2015
- 2015-11-10 AR ARP150103665A patent/AR102612A1/es unknown
- 2015-11-10 TW TW104137007A patent/TW201617451A/zh unknown
- 2015-11-11 MX MX2017004727A patent/MX2017004727A/es unknown
- 2015-11-11 RU RU2017112973A patent/RU2017112973A/ru not_active Application Discontinuation
- 2015-11-11 JP JP2017520501A patent/JP2017532963A/ja active Pending
- 2015-11-11 EP EP15858265.0A patent/EP3218501A4/en not_active Withdrawn
- 2015-11-11 AU AU2015346442A patent/AU2015346442B2/en not_active Ceased
- 2015-11-11 US US14/938,632 patent/US20190002905A9/en not_active Abandoned
- 2015-11-11 CN CN201580055915.5A patent/CN106852156A/zh active Pending
- 2015-11-11 WO PCT/US2015/060134 patent/WO2016077449A1/en not_active Ceased
- 2015-11-11 CA CA2964498A patent/CA2964498A1/en not_active Abandoned
- 2015-11-11 KR KR1020177009871A patent/KR20170081168A/ko not_active Withdrawn
- 2015-11-11 BR BR112017007446A patent/BR112017007446A2/pt not_active Application Discontinuation
-
2017
- 2017-04-06 CO CONC2017/0003326A patent/CO2017003326A2/es unknown
- 2017-04-12 IL IL251704A patent/IL251704A0/en unknown
- 2017-04-12 PH PH12017500698A patent/PH12017500698A1/en unknown
- 2017-04-13 CL CL2017000922A patent/CL2017000922A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CA2964498A1 (en) | 2016-05-19 |
| PH12017500698A1 (en) | 2017-10-09 |
| EP3218501A1 (en) | 2017-09-20 |
| EP3218501A4 (en) | 2018-05-02 |
| AR102612A1 (es) | 2017-03-15 |
| BR112017007446A2 (pt) | 2017-12-19 |
| CO2017003326A2 (es) | 2017-07-11 |
| CL2017000922A1 (es) | 2017-11-10 |
| US20160130594A1 (en) | 2016-05-12 |
| TW201617451A (zh) | 2016-05-16 |
| US20190002905A9 (en) | 2019-01-03 |
| AU2015346442B2 (en) | 2018-08-16 |
| JP2017532963A (ja) | 2017-11-09 |
| KR20170081168A (ko) | 2017-07-11 |
| CN106852156A (zh) | 2017-06-13 |
| IL251704A0 (en) | 2017-06-29 |
| MX2017004727A (es) | 2017-07-27 |
| AU2015346442A1 (en) | 2017-04-13 |
| WO2016077449A1 (en) | 2016-05-19 |
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