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RU2017112086A - COMBINED PROBIOTIC DRUG BASED ON SPORO-FORMING BACTERIA OF THE GENUS BACILLUS FOR USE IN ANIMAL BREEDING, METHOD OF ITS PRODUCTION - Google Patents

COMBINED PROBIOTIC DRUG BASED ON SPORO-FORMING BACTERIA OF THE GENUS BACILLUS FOR USE IN ANIMAL BREEDING, METHOD OF ITS PRODUCTION Download PDF

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RU2017112086A
RU2017112086A RU2017112086A RU2017112086A RU2017112086A RU 2017112086 A RU2017112086 A RU 2017112086A RU 2017112086 A RU2017112086 A RU 2017112086A RU 2017112086 A RU2017112086 A RU 2017112086A RU 2017112086 A RU2017112086 A RU 2017112086A
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vkm
bacillus subtilis
biomass
strains
bacillus
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RU2017112086A
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RU2017112086A3 (en
RU2675934C2 (en
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Вахтанг Витальевич Джавахия
Елена Викторовна Глаголева
Татьяна Михайловна Воинова
Максим Игоревич Карташов
Александр Игоревич Овчинников
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Общество с ограниченной ответственностью "Фермлаб"
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Priority to RU2017112086A priority Critical patent/RU2675934C2/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/742—Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Molecular Biology (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Claims (14)

1. Комбинированный пробиотический препарат для использования в животноводстве, содержащий смесь биомассы штаммов бактерий Bacillus subtilis и Bacillus licheniformis в споровой форме, целевые добавки и наполнитель - сухую молочную сыворотку, отличающийся тем, что он содержит смесь биомассы штаммов бактерий Bacillus subtilis ВКМ-B-2998D, Bacillus licheniformis ВКМ-B-2999D в соотношении 1:1 с содержанием жизнеспособных спор не менее 1,0×1012 КОЕ/г., а в качестве добавок - биомассу штамма Bacillus subtilis (natto) ВКМ B-3057D с содержанием жизнеспособных спор не менее 1,0×1012 КОЕ/г. и глюконат кальция в соотношении ингредиентов, % масс:1. The combined probiotic preparation for use in animal husbandry, containing a mixture of biomass of the bacterial strains of Bacillus subtilis and Bacillus licheniformis in spore form, target additives and excipient is dry milk whey, characterized in that it contains a mixture of biomass of the bacterial strains of Bacillus subtilis VKM-B-2998D , Bacillus licheniformis VKM-B-2999D in a 1: 1 ratio with a viable spore content of at least 1.0 × 10 12 CFU / g, and as additives, the biomass of the Bacillus subtilis (natto) VKM B-3057D strain with viable spore content not less than 1.0 × 10 12 CFU / g. and calcium gluconate in the ratio of ingredients,% of the mass: - смесь(1:1) биомассы штаммов (В. subtilis, В. licheniformis)- a mixture (1: 1) of the biomass of strains (B. subtilis, B. licheniformis) - 0.35%,- 0.35%, - биомасса штамма В. subtilis (natto) ВКМ B-3057D- biomass of strain B. subtilis (natto) VKM B-3057D - 0.15%,- 0.15%, - глюконат кальция- calcium gluconate - 0.1%,- 0.1% - молочная сыворотка- milk serum - до 100%.- up to 100%.
2. Пробиотический препарат по п. 1, отличающийся тем, что количество жизнеспособных бактерий в готовом препарате составляет не менее 5×109 спор/г.2. The probiotic preparation according to claim 1, characterized in that the number of viable bacteria in the finished preparation is at least 5 × 10 9 spores / g. 3. Пробиотический препарат по п. 1, отличающийся тем, что штаммы бактерий Bacillus subtilis ВКМ-B-2998D, Bacillus licheniformis ВКМ-B-2999D, Bacillus subtilis (natto) ВКМ B-3057D получают путем ненаправленного многоступенчатого УФ мутагенеза и селекции.3. The probiotic preparation according to claim 1, characterized in that the bacterial strains Bacillus subtilis VKM-B-2998D, Bacillus licheniformis VKM-B-2999D, Bacillus subtilis (natto) VKM B-3057D are obtained by non-directional multi-stage UV mutagenesis and selection. 4. Способ получения пробиотического препарата по п. 1, включающий получение штаммов бактерий Bacillus subtilis ВКМ-B-2998D, Bacillus licheniformis ВКМ-B-2999D и штамма Bacillus subtilis (natto) ВКМ B-3057D, последующее раздельное культивирование штаммов на мелассно-кукурузной среде при рН 6,8-7,0, концентрирование культуральных жидкостей, сушку, смешивание полученной биомассы штаммов Bacillus subtilis ВКМ-B-2998D, Bacillus licheniformis ВКМ-B-2999D в споровой форме между собой в соотношении 1:1 и затем со штаммом Bacillus subtilis (natto) ВКМ B-3057D в количестве 0.15% от массы препарата и глюконатом кальция в количестве 0,1 масс. % от массы препарата с последующей добавкой сухой молочной сыворотки до 100% массы препарата, приводящей к получению препарата с содержанием живых микроорганизмов не менее 5×109 спор/г.4. A method of obtaining a probiotic preparation according to claim 1, comprising obtaining strains of bacteria Bacillus subtilis VKM-B-2998D, Bacillus licheniformis VKM-B-2999D and strain Bacillus subtilis (natto) VKM B-3057D, subsequent separate cultivation of the strains on molasses corn medium at pH 6.8-7.0, concentration of culture liquids, drying, mixing the obtained biomass of strains of Bacillus subtilis VKM-B-2998D, Bacillus licheniformis VKM-B-2999D in spore form with each other in a ratio of 1: 1 and then with the strain Bacillus subtilis (natto) VKM B-3057D in an amount of 0.15% by weight of the drug and calcium gluconate in an amount of 0.1 mass . % by weight of the drug, followed by the addition of whey powder to 100% by weight of the drug, resulting in a preparation with a living microorganism content of at least 5 × 10 9 spores / g. 5. Комбинированный пробиотический препарат для использования в животноводстве, содержащий смесь биомассы штаммов бактерий Bacillus subtilis и Bacillus licheniformis в споровой форме, целевую добавку и наполнитель - сухую молочную сыворотку, отличающийся тем, что он содержит смесь биомассы штаммов бактерий Bacillus subtilis ВКМ-B-2998D,5. Combined probiotic preparation for use in animal husbandry, containing a mixture of biomass of the bacterial strains of Bacillus subtilis and Bacillus licheniformis in spore form, the target additive and excipient is dry milk whey, characterized in that it contains a mixture of biomass of the bacterial strains of Bacillus subtilis VKM-B-2998D , Bacillus licheniformis ВКМ-B-2999D в соотношении 1:1 с содержанием жизнеспособных спор не менее 1,0×1012 КОЕ/г, а в качестве добавки - ферментный препарат Кемзайм Плюс в соотношении ингредиентов, % масс:Bacillus licheniformis VKM-B-2999D in a 1: 1 ratio with a viable spore content of at least 1.0 × 10 12 CFU / g, and as an additive, the enzyme preparation Kemsaym Plus in the ratio of ingredients,% of the mass: - смесь (1:1) биомассы штаммов (В. subtilis, В. licheniformis)- a mixture (1: 1) of the biomass of strains (B. subtilis, B. licheniformis) - 0,5%,- 0.5% - ферментный препарат Кемзайм Плюс- enzyme preparation Kemsaym Plus - 5%,- 5%, - молочная сыворотка- milk serum - до 100%.- up to 100%.
6. Пробиотический препарат по п. 5, отличающийся тем, что количество жизнеспособных бактерий в готовом препарате составляет не менее 5×109 спор/г.6. The probiotic preparation according to claim 5, characterized in that the number of viable bacteria in the finished preparation is at least 5 × 10 9 spores / g. 7. Пробиотический препарат по п. 5, отличающийся тем, что штаммы бактерий Bacillus subtilis ВКМ-B-2998D и Bacillus licheniformis ВКМ-В-2999D получают путем ненаправленного многоступенчатого УФ мутагенеза и селекции.7. The probiotic preparation according to claim 5, characterized in that the bacterial strains Bacillus subtilis VKM-B-2998D and Bacillus licheniformis VKM-B-2999D are obtained by omnidirectional multi-stage UV mutagenesis and selection. 8. Способ получения пробиотического препарата по п. 5, включающий получение шаммов бактерий Bacillus subtilis ВКМ-B-2998D и Bacillus licheniformis ВКМ-B-2999D, последующее раздельное культивирование штаммов на мелассно-кукурузной среде при рН 6,8-7,0, концентрирование культуральных жидкостей, сушку, смешивание полученной биомассы штаммов в споровой форме между собой в соотношении 1:1 и затем с ферментным препаратом Кемзайм Плюс в количестве 5% от массы препарата с последующей добавкой сухой молочной сыворотки до 100% массы препарата, приводящей к получению препарата с содержанием живых микроорганизмов не менее 5×109 спор/г.8. A method for producing a probiotic preparation according to claim 5, comprising obtaining bacterial shamma Bacillus subtilis VKM-B-2998D and Bacillus licheniformis VKM-B-2999D, subsequent separate cultivation of the strains on molasses-corn medium at pH 6.8-7.0, the concentration of culture fluids, drying, mixing the obtained biomass of the strains in spore form with each other in a ratio of 1: 1 and then with the enzyme preparation Kemsaym Plus in the amount of 5% by weight of the drug, followed by the addition of dry whey to 100% of the weight of the drug, resulting in the preparation with s holding the living microorganisms have not less than 5 × 10 September spores / g. 9. Штамм Bacillus subtilis ВКМ-В-29980, используемый в качестве компонента нового комбинированного пробиотического препарата.9. The strain Bacillus subtilis VKM-B-29980, used as a component of a new combined probiotic preparation. 10 Штамм Bacillus licheniformis ВКМ-B-2999D, используемый в качестве компонента нового комбинированного пробиотического препарата.10 The strain of Bacillus licheniformis VKM-B-2999D, used as a component of a new combined probiotic preparation. 11. Штамм Bacillus subtilis (natto) ВКМ B-3057D, используемый в качестве добавки к новому комбинированному пробиотическому препарату.11. The strain Bacillus subtilis (natto) VKM B-3057D, used as an additive to the new combined probiotic preparation.
RU2017112086A 2017-04-10 2017-04-10 Combined probiotic preparation based on spore-forming bacteria of bacillus genus (options) for use in animal breeding, method for production thereof (variants) and bacillus subtilis (natto) strain used as additive for preparation RU2675934C2 (en)

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RU2768281C1 (en) * 2021-05-24 2022-03-23 Общество с ограниченной ответственностью "Алтайская биотехнологическая компания" (ООО "Алтбиотех") Combined probiotic for aquaculture based on spore-forming bacteria of the genus bacillus and a method for production thereof
CN113575758A (en) * 2021-08-04 2021-11-02 广西大学 Composite probiotics and preparation method thereof

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RU2432393C1 (en) * 2010-08-26 2011-10-27 Общество с ограниченной ответственностью "Национальная биотехнологическая компания" METHOD TO PRODUCE PROBIOTIC PREPARATION BASED ON SPOROGENOUS STRAINS Bac subtillis AND Bac licheniformis
RU2475535C1 (en) * 2011-09-14 2013-02-20 Общество с ограниченной ответственностью "Фермлаб" Method to produce probiotic preparation lacto-amylovorin

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