RU2008105576A - METHOD FOR PRODUCING MEDICINAL AND PREVENTIVE PROBIOTIC ADDITIVES - Google Patents
METHOD FOR PRODUCING MEDICINAL AND PREVENTIVE PROBIOTIC ADDITIVES Download PDFInfo
- Publication number
- RU2008105576A RU2008105576A RU2008105576/13A RU2008105576A RU2008105576A RU 2008105576 A RU2008105576 A RU 2008105576A RU 2008105576/13 A RU2008105576/13 A RU 2008105576/13A RU 2008105576 A RU2008105576 A RU 2008105576A RU 2008105576 A RU2008105576 A RU 2008105576A
- Authority
- RU
- Russia
- Prior art keywords
- bacteria
- agar
- nutrient medium
- preparation
- culture medium
- Prior art date
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- 239000006041 probiotic Substances 0.000 title claims abstract 3
- 230000000529 probiotic effect Effects 0.000 title claims abstract 3
- 235000018291 probiotics Nutrition 0.000 title claims abstract 3
- 239000000654 additive Substances 0.000 title 1
- 238000004519 manufacturing process Methods 0.000 title 1
- 230000003449 preventive effect Effects 0.000 title 1
- 241000894006 Bacteria Species 0.000 claims abstract 12
- 239000002609 medium Substances 0.000 claims abstract 10
- 235000015097 nutrients Nutrition 0.000 claims abstract 10
- 239000001963 growth medium Substances 0.000 claims abstract 6
- 238000002360 preparation method Methods 0.000 claims abstract 6
- 229920001817 Agar Polymers 0.000 claims abstract 4
- 241000206672 Gelidium Species 0.000 claims abstract 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract 4
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract 4
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract 4
- 239000001888 Peptone Substances 0.000 claims abstract 4
- 108010080698 Peptones Proteins 0.000 claims abstract 4
- 241000186428 Propionibacterium freudenreichii Species 0.000 claims abstract 4
- 235000010419 agar Nutrition 0.000 claims abstract 4
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims abstract 4
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims abstract 4
- 235000011130 ammonium sulphate Nutrition 0.000 claims abstract 4
- 229940041514 candida albicans extract Drugs 0.000 claims abstract 4
- KTVIXTQDYHMGHF-UHFFFAOYSA-L cobalt(2+) sulfate Chemical compound [Co+2].[O-]S([O-])(=O)=O KTVIXTQDYHMGHF-UHFFFAOYSA-L 0.000 claims abstract 4
- 239000012153 distilled water Substances 0.000 claims abstract 4
- 239000008103 glucose Substances 0.000 claims abstract 4
- 238000011534 incubation Methods 0.000 claims abstract 4
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract 4
- 239000008101 lactose Substances 0.000 claims abstract 4
- 238000000034 method Methods 0.000 claims abstract 4
- 235000019319 peptone Nutrition 0.000 claims abstract 4
- 238000009331 sowing Methods 0.000 claims abstract 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract 4
- 239000012138 yeast extract Substances 0.000 claims abstract 4
- 235000013372 meat Nutrition 0.000 claims abstract 3
- 230000001580 bacterial effect Effects 0.000 claims abstract 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims abstract 2
- 235000013305 food Nutrition 0.000 claims abstract 2
- 239000000047 product Substances 0.000 claims abstract 2
- 230000000069 prophylactic effect Effects 0.000 claims abstract 2
- 241000894007 species Species 0.000 claims abstract 2
- 239000013589 supplement Substances 0.000 claims abstract 2
- 230000001225 therapeutic effect Effects 0.000 claims abstract 2
- 229940044175 cobalt sulfate Drugs 0.000 claims 2
- 229910000361 cobalt sulfate Inorganic materials 0.000 claims 2
- 230000002255 enzymatic effect Effects 0.000 claims 2
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims 1
- 240000008042 Zea mays Species 0.000 claims 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims 1
- 235000005822 corn Nutrition 0.000 claims 1
- 239000001166 ammonium sulphate Substances 0.000 abstract 1
- 239000000306 component Substances 0.000 abstract 1
- 238000000855 fermentation Methods 0.000 abstract 1
- 230000004151 fermentation Effects 0.000 abstract 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
Abstract
1. Способ получения лечебно-профилактической пробиотической добавки в корм, включающий приготовление питательной среды, введение в нее посевных доз штаммов бактерий: Lactobacillus plantarum и Propionibacterium freudenreichii с последующим их культивированием до получения готового продукта, отличающийся тем, что в качестве Lactobacillus plantarum используют штамм ВКПМ В-2347, в качестве Propionibacterium freudenreichii используют штамм ВКПМ В-6561, посевная доза каждого вида указанных бактерий составляет не менее 1·106 КОЕ в 1 см3, питательная среда содержит воду дистиллированную, глюкозу, пептон мясной ферментативный, дрожжевой экстракт, кукурузно-лактозную питательную среду, кобальт сернокислый, аммоний сернокислый, агар-агар при следующем содержании компонентов питательной среды: ! Вода дистиллированная1000 млГлюкоза29-31 гПептон мясной ферментативный9-11 гДрожжевой экстракт4,5-5,5 гКукурузно-лактозная питательная среда (ГМК)1,9-2,1 гКобальт сернокислый0,24-,26 гАммоний сернокислый4,5-5,5 гАгар-агар0,9-1,1 г, ! а инкубирование бактерий в питательной среде осуществляют до достижения ими титра не менее 1·106 КОЕ в 1 см3. ! 2. Способ по п.1, отличающийся тем, что посевная доза бактерий составляет для получения препарата во флаконах по 9 мл каждого вида бактерий на 300 мл питательной среды, а для получения препарата в бутылях по 300 мл каждого вида бактерий на 40000 см3 питательной среды, причем инкубирование бактерий осуществляют при температуре (37,0±0,5)°С в течение 18-20 ч.1. A method of obtaining a therapeutic and prophylactic probiotic supplement in food, including the preparation of a nutrient medium, the introduction of inoculated doses of bacterial strains: Lactobacillus plantarum and Propionibacterium freudenreichii with their subsequent cultivation to obtain the finished product, characterized in that the VKPM strain is used as Lactobacillus plantarum B-2347, the strain VKPM B-6561 is used as Propionibacterium freudenreichii, the sowing dose of each species of these bacteria is at least 1 · 10 6 CFU in 1 cm3, the nutrient medium contains distilled water, glucose, peptone clear fermentation, yeast extract, corn-lactose culture medium, cobalt sulphate, ammonium sulphate, agar-agar at the following content of the culture medium components: Distilled water 1000 ml Glucose 29-31 g Meat fermentative peptone 9-11 g Yeast extract 4.5-5.5 g Corn-lactose growth medium (GMC) 1.9-2.1 g Sulfate cobalt 0.24-, 26 g Ammonium sulfate 4.5-5.5 g Agar- agar 0.9-1.1 g,! and the incubation of bacteria in a nutrient medium is carried out until they reach a titer of at least 1 · 10 6 CFU in 1 cm3. ! 2. The method according to claim 1, characterized in that the sowing dose of bacteria is for the preparation in bottles of 9 ml of each type of bacteria per 300 ml of culture medium, and for the preparation in bottles for 300 ml of each type of bacteria per 40,000 cm3 of culture medium moreover, the incubation of bacteria is carried out at a temperature of (37.0 ± 0.5) ° C for 18-20 hours
Claims (2)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2008105576/13A RU2378000C2 (en) | 2008-02-13 | 2008-02-13 | Method for production of therapeutic additive |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2008105576/13A RU2378000C2 (en) | 2008-02-13 | 2008-02-13 | Method for production of therapeutic additive |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2008105576A true RU2008105576A (en) | 2009-08-20 |
| RU2378000C2 RU2378000C2 (en) | 2010-01-10 |
Family
ID=41150732
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2008105576/13A RU2378000C2 (en) | 2008-02-13 | 2008-02-13 | Method for production of therapeutic additive |
Country Status (1)
| Country | Link |
|---|---|
| RU (1) | RU2378000C2 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2423518C1 (en) * | 2010-01-27 | 2011-07-10 | Государственное образовательное учреждение высшего профессионального образования Воронежская государственная технологическая академия (ГОУ ВПО ВГТА) | Method for production of frozen full-ration dog feed with probiotic microflora |
| RU2541646C1 (en) * | 2013-10-09 | 2015-02-20 | Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования Новосибирский государственный аграрный университет | Method of improving quality of fur and production of rabbit husbandry |
| RU2692609C1 (en) * | 2018-06-13 | 2019-06-25 | Федеральное государственное бюджетное научное учреждение "Всероссийский научно-исследовательский институт использования техники и нефтепродуктов в сельском хозяйстве" (ФГБНУ ВНИИТиН) | Phyto-zymotic fodder additive for calves |
| RU2686326C1 (en) * | 2018-07-11 | 2019-04-25 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Кубанский государственный аграрный университет имени И.Т. Трубилина" | Nutrient medium for cultivation of lactobacilli |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2054881C1 (en) * | 1995-06-29 | 1996-02-27 | Акционерное общество открытого типа "Кстовский опытно-промышленный завод белково-витаминных концентратов" | Method of preparing food protein addition |
| RU2083666C1 (en) * | 1995-07-27 | 1997-07-10 | Ганина Вера Ивановна | Method of preparing the dry bacterial preparation |
| RU2250265C2 (en) * | 2003-06-27 | 2005-04-20 | Общество с ограниченной ответственностью "Биотех-Инжиниринг" | Method for production of feedstuff |
| RU2243678C1 (en) * | 2003-07-02 | 2005-01-10 | Общество с ограниченной ответственностью "Биотех-Инжиниринг" | Method for preparing protein-vitamin fodder |
-
2008
- 2008-02-13 RU RU2008105576/13A patent/RU2378000C2/en active
Also Published As
| Publication number | Publication date |
|---|---|
| RU2378000C2 (en) | 2010-01-10 |
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