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RU2016129435A - Молекулы нуклеиновой кислоты phkп ii-140, которые придают устойчивость к жестококрылым насекомым-вредителям - Google Patents

Молекулы нуклеиновой кислоты phkп ii-140, которые придают устойчивость к жестококрылым насекомым-вредителям Download PDF

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RU2016129435A
RU2016129435A RU2016129435A RU2016129435A RU2016129435A RU 2016129435 A RU2016129435 A RU 2016129435A RU 2016129435 A RU2016129435 A RU 2016129435A RU 2016129435 A RU2016129435 A RU 2016129435A RU 2016129435 A RU2016129435 A RU 2016129435A
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rna
nucleic acid
group
rnapii
diabrotica
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RU2016129435A
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Кеннет Э. НАРВА
Каника АРОРА
Муругесан РАНГАСАМИ
Баладжи ВЕРАМАНИ
Премчанд ГАНДРА
Сара И. ВОРДЕН
Хуажун ЛИ
Андреас ФИЛЬЦИНСКАС
Айлен КНОРР
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ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи
Фраунхофер-Гезелльшафт Цур Фердерунг Дер Ангевандтен Форшунг Е.Ф.
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
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    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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Claims (33)

1. РНК, содержащая по меньшей мере 15 смежных нуклеотидов, обладающих на всем протяжении по меньшей мере 80% идентичностью с последовательностью ДНК, кодирующей по меньшей мере участок rnapII-140.
2. РНК по п. 1, где РНК содержит стебель и петлю.
3. РНК по п. 1, где нуклеиновая кислота дополнительно содержит последовательность, комплементарную по меньшей мере 15 смежным нуклеотидам.
4. РНК по п. 2, где по меньшей мере участок стебля стебля-петли экспрессируемой РНК образован связыванием по меньшей мере 15 смежных нуклеотидов с последовательностью, комплементарной по меньшей мере 15 смежным нуклеотидам.
5. РНК по п. 1, где РНК представляет собой иРНК
6. РНК по п. 5, где иРНК представляет собой дцРНК, миРНК, мкРНК или вмРНК.
7. РНК по п. 1, где РНК содержит аналог нуклеиновой кислоты.
8. РНК по п. 1, где РНК способна ингибировать экспрессию РНКП II-140 у жесткокрылых насекомых.
9. РНК по п. 8, где ингибирование экспрессии РНКП II-140 происходит в результате посттранскрипционого сайленсинга гена.
10. РНК по п. 1, где rnapII-140 представляет собой rnapII-140 Diabrotica virgifera virgifera.
11. РНК по п. 1, где rnapII-140 кодируется SEQ ID NO:1.
12. РНК по п. 1, где rnapII-140 представляет собой rnapII-140 Meligethes.
13. РНК по п. 1, где rnapII-140 кодируется нуклеиновой кислотой, выбранной из группы, состоящей из SEQ ID NO:73, 75 и 81.
14. РНК по п. 1, где последовательность, кодирующая по меньшей мере участок rnapII-140, выбрана из группы, состоящей из SEQ ID NO:3, 4, 5 и 77.
15. РНК по п. 1, где по меньшей мере 15 смежных нуклеотидов выбраны из группы, состоящей из 19, 21, 25, 29, 30, 40, 50, 70 и 100 смежных нуклеотидов.
16. РНК по п. 1, где по меньшей мере 80% идентичности выбраны из группы, состоящей из 85%, 90%, 95% и 100% идентичности.
17. Растительная клетка, содержащая РНК по п. 1.
18. Растительная клетка по п. 15, где растение выбрано из группы, состоящей из Zea mays, Glycine max, Brassica spp. и семейства Poaceae.
19. Нуклеиновая кислота, кодирующая РНК по п. 1.
20. Нуклеиновая кислота по п. 17, где нуклеиновая кислота содержит последовательность, выбранную из группы, состоящей из SEQ ID NO:16, 17, 78 и 79.
21. Нуклеиновая кислота по п. 17, где нуклеиновая кислота является функционально связанной с промотором.
22. Вектор, содержащий нуклеиновую кислоту по п. 17.
23. Растительная клетка, содержащая нуклеиновую кислоту по п. 17.
24. Растительная клетка по п. 21, где растение выбрано из группы, состоящей из Zea mays, Glycine max, Brassica spp. и семества Poaceae.
25. Растительная клетка по п. 21, где нуклеиновую кислоту по п. 17 вводят в геном растительной клетки.
26. Способ борьбы с жесткокрылыми насекомыми, где способ включает экспрессию нуклеиновой кислоты по п. 17 в растении.
27. Способ по п. 24, где жесткокрылое насекомое выбрано из группы, состоящей из Diabrotica virgifera virgifera, Diabrotica barberi, Diabrotica undecimpunctata howardi, Diabrotica virgifera zeae, Diabrotica balteata, Diabrotica undecimpunctata tenella, Diabrotica undecimpunctata undecimpunctata и Meligethes aeneus.
28. Источник пищи для жесткокрылого насекомого, содержащий РНК по п. 1.
29. Способ борьбы с жесткокрылыми насекомыми, где способ включает кормление жесткокрылого насекомого источником пиши по п. 26.
30. Способ по п. 24, дополнительно включающий экспрессию нуклеиновой кислоты, кодирующую инсектицидный белок Bacillus thuringiensis.
31. Способ по п. 28, где белок-токсин Bacillus thuringiensis выбран из группы, состоящей из Cry35Ab1, Cry34/35Ab10, Cry3A, Cry3B, Cry6A и их сочетаний.
32. Способ по п. 24, дополнительно включающий экспрессию нуклеиновой кислоты, кодирующей РНКи, которая ингибирует экспрессию гена жесткокрылого насекомого.
33. Способ по п. 30, где нуклеиновая кислота выбрана из группы, состоящей из Caf1-180, VatpaseC, Rho1, VatpaseH, PPI-87B, RPA7 и RPS6.
RU2016129435A 2013-12-20 2014-12-19 Молекулы нуклеиновой кислоты phkп ii-140, которые придают устойчивость к жестококрылым насекомым-вредителям RU2016129435A (ru)

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US201361919239P 2013-12-20 2013-12-20
US61/919,239 2013-12-20
PCT/US2014/071583 WO2015095750A1 (en) 2013-12-20 2014-12-19 Rnapii-140 nucleic acid molecules that confer resistance to coleopteran pests

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CN (1) CN106028838A (ru)
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