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RU2014114039A - Дифференцирование человеческих эмбриональных стволовых клеток в линию панкреатических эндокринных клеток - Google Patents

Дифференцирование человеческих эмбриональных стволовых клеток в линию панкреатических эндокринных клеток Download PDF

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RU2014114039A
RU2014114039A RU2014114039/10A RU2014114039A RU2014114039A RU 2014114039 A RU2014114039 A RU 2014114039A RU 2014114039/10 A RU2014114039/10 A RU 2014114039/10A RU 2014114039 A RU2014114039 A RU 2014114039A RU 2014114039 A RU2014114039 A RU 2014114039A
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Алиреза РЕЗАНИА
Бенджамин ФРАЙЕР
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Abstract

1. Способ увеличения экспрессии инсулина и MAFA в клетках, экспрессирующих маркеры, характерные для линии панкреатических эндокринных клеток, где способ включает стадии:а. последовательной дифференцировки плюрипотентных стволовых клеток человека с получением клеток, экспрессирующих маркеры, характерные для линии панкреатических эндокринных клеток;b. культивирования клеток, экспрессирующих маркеры, характерные для линии панкреатических эндокринных клеток, в среде, содержащей добавленное количество ингибитора циклин-зависимой киназы, чтобы вызвать увеличение экспрессии инсулина и MAFA по сравнению с клетками, экспрессирующими маркеры, характерные для линии панкреатических эндокринных клеток, которые не культивируют в среде, содержащей добавленное количество ингибитора циклин-зависимой киназы;где ингибитор циклин-зависимой киназы представляет собой ингибитор CDK 1/2.2. Способ по п. 1, где ингибитор представляет собой 5-амино-3-((4-(аминосульфонил)фенил)амино)-N-(2,6,-дифторфенил)-1H-1,2,4-триазол-1-каботиамид.3. Способ увеличения экспрессии инсулина и MAFA в клетках, экспрессирующих маркеры, характерные для линии панкреатических эндокринных клеток, где способ включает стадии:а. последовательной дифференцировки плюрипотентных стволовых клеток человека с получением клеток, экспрессирующих маркеры, характерные для линии панкреатических эндокринных клеток;b. культивирования клеток, экспрессирующих маркеры,характерные для линии панкреатических эндокринных клеток, в среде, содержащей добавленное количество ингибитора циклин-зависимой киназы, чтобы вызвать увеличение экспрессии инсулина и MAFA по сравнению с клетками, эк�

Claims (4)

1. Способ увеличения экспрессии инсулина и MAFA в клетках, экспрессирующих маркеры, характерные для линии панкреатических эндокринных клеток, где способ включает стадии:
а. последовательной дифференцировки плюрипотентных стволовых клеток человека с получением клеток, экспрессирующих маркеры, характерные для линии панкреатических эндокринных клеток;
b. культивирования клеток, экспрессирующих маркеры, характерные для линии панкреатических эндокринных клеток, в среде, содержащей добавленное количество ингибитора циклин-зависимой киназы, чтобы вызвать увеличение экспрессии инсулина и MAFA по сравнению с клетками, экспрессирующими маркеры, характерные для линии панкреатических эндокринных клеток, которые не культивируют в среде, содержащей добавленное количество ингибитора циклин-зависимой киназы;
где ингибитор циклин-зависимой киназы представляет собой ингибитор CDK 1/2.
2. Способ по п. 1, где ингибитор представляет собой 5-амино-3-((4-(аминосульфонил)фенил)амино)-N-(2,6,-дифторфенил)-1H-1,2,4-триазол-1-каботиамид.
3. Способ увеличения экспрессии инсулина и MAFA в клетках, экспрессирующих маркеры, характерные для линии панкреатических эндокринных клеток, где способ включает стадии:
а. последовательной дифференцировки плюрипотентных стволовых клеток человека с получением клеток, экспрессирующих маркеры, характерные для линии панкреатических эндокринных клеток;
b. культивирования клеток, экспрессирующих маркеры,
характерные для линии панкреатических эндокринных клеток, в среде, содержащей добавленное количество ингибитора циклин-зависимой киназы, чтобы вызвать увеличение экспрессии инсулина и MAFA по сравнению с клетками, экспрессирующими маркеры, характерные для линии панкреатических эндокринных клеток, которые не культивируют в среде, содержащей добавленное количество ингибитора циклин-зависимой киназы;
где ингибитор циклин-зависимой киназы представляет собой ингибитор CDK 4.
4. Способ по п. 3, где ингибитор 2-бром-12,13-дигидро-5Н-индол[2,3-а]пирроло[3,4-с]карбазол-5,7,(6Н)дион.
RU2014114039A 2008-10-31 2014-04-09 Дифференцирование человеческой эмбриональной стволовой клетки в линию панкреатических эндокринных клеток RU2664226C2 (ru)

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