RU2007114379A - METHOD FOR ISOLATING BIOLUMINESCENT BACTERIA - Google Patents
METHOD FOR ISOLATING BIOLUMINESCENT BACTERIA Download PDFInfo
- Publication number
- RU2007114379A RU2007114379A RU2007114379/13A RU2007114379A RU2007114379A RU 2007114379 A RU2007114379 A RU 2007114379A RU 2007114379/13 A RU2007114379/13 A RU 2007114379/13A RU 2007114379 A RU2007114379 A RU 2007114379A RU 2007114379 A RU2007114379 A RU 2007114379A
- Authority
- RU
- Russia
- Prior art keywords
- nutrient
- bacteria
- bioluminescent bacteria
- isolation
- sodium chloride
- Prior art date
Links
- 241000894006 Bacteria Species 0.000 title claims abstract 9
- 238000000034 method Methods 0.000 title claims abstract 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract 8
- 235000015097 nutrients Nutrition 0.000 claims abstract 8
- 238000002955 isolation Methods 0.000 claims abstract 4
- 239000002609 medium Substances 0.000 claims abstract 4
- 239000013535 sea water Substances 0.000 claims abstract 4
- 239000011780 sodium chloride Substances 0.000 claims abstract 4
- 239000012153 distilled water Substances 0.000 claims abstract 2
- 239000012528 membrane Substances 0.000 claims abstract 2
- 239000006916 nutrient agar Substances 0.000 claims abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract 2
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Способ выделения биолюминесцентных бактерий, предусматривающий приготовление питательной среды, включающей дистиллированную воду, хлористый натрий, питательную основу, посев на нее культур бактерий, сконцентрированных из морской воды на мембранном фильтре, и выращивание при комнатной температуре в течение 22-24 ч, с последующим выделением чистых культур биолюминесцентных бактерий, отличающийся тем, что определяют соленость морской воды в районе выделения биолюминесцентных бактерий, и хлористый натрий добавляют в питательную среду в соответствии с определенным значением солености, а в качестве питательной основы используют сухой питательный агар в количестве 34,0-36,0 г/л.A method for isolating bioluminescent bacteria, comprising preparing a nutrient medium including distilled water, sodium chloride, a nutrient base, inoculating bacteria cultures concentrated from sea water on a membrane filter, and growing at room temperature for 22-24 hours, followed by isolation of pure cultures of bioluminescent bacteria, characterized in that they determine the salinity of seawater in the area of isolation of bioluminescent bacteria, and sodium chloride is added to the nutrient medium in otvetstvii a certain salinity value, and as the nutrient bases used dry nutrient agar in an amount of 34,0-36,0 g / l.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2007114379/13A RU2358009C2 (en) | 2007-04-16 | 2007-04-16 | Method of bioluminescent bacteria separation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2007114379/13A RU2358009C2 (en) | 2007-04-16 | 2007-04-16 | Method of bioluminescent bacteria separation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2007114379A true RU2007114379A (en) | 2008-10-27 |
| RU2358009C2 RU2358009C2 (en) | 2009-06-10 |
Family
ID=41024909
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2007114379/13A RU2358009C2 (en) | 2007-04-16 | 2007-04-16 | Method of bioluminescent bacteria separation |
Country Status (1)
| Country | Link |
|---|---|
| RU (1) | RU2358009C2 (en) |
-
2007
- 2007-04-16 RU RU2007114379/13A patent/RU2358009C2/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| RU2358009C2 (en) | 2009-06-10 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20120417 |