RU2004118303A - Быстросозревающие флуоресцирующие белки и способы их применения - Google Patents
Быстросозревающие флуоресцирующие белки и способы их применения Download PDFInfo
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- RU2004118303A RU2004118303A RU2004118303/13A RU2004118303A RU2004118303A RU 2004118303 A RU2004118303 A RU 2004118303A RU 2004118303/13 A RU2004118303/13 A RU 2004118303/13A RU 2004118303 A RU2004118303 A RU 2004118303A RU 2004118303 A RU2004118303 A RU 2004118303A
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- nucleic acid
- protein
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- 108090000623 proteins and genes Proteins 0.000 title claims abstract 29
- 102000004169 proteins and genes Human genes 0.000 title claims abstract 29
- 150000007523 nucleic acids Chemical class 0.000 claims abstract 25
- 102000039446 nucleic acids Human genes 0.000 claims abstract 24
- 108020004707 nucleic acids Proteins 0.000 claims abstract 24
- 108091006047 fluorescent proteins Proteins 0.000 claims abstract 6
- 102000034287 fluorescent proteins Human genes 0.000 claims abstract 6
- 241000243321 Cnidaria Species 0.000 claims abstract 5
- 241000242757 Anthozoa Species 0.000 claims abstract 4
- 230000009261 transgenic effect Effects 0.000 claims abstract 3
- 239000012634 fragment Substances 0.000 claims abstract 2
- 108700019146 Transgenes Proteins 0.000 claims 2
- 108010021843 fluorescent protein 583 Proteins 0.000 claims 2
- 230000035772 mutation Effects 0.000 claims 2
- 239000002243 precursor Substances 0.000 claims 2
- 241000006867 Discosoma Species 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 238000000034 method Methods 0.000 claims 1
- 230000005026 transcription initiation Effects 0.000 claims 1
- 230000005030 transcription termination Effects 0.000 claims 1
- 239000000203 mixture Substances 0.000 abstract 3
- 241000894007 species Species 0.000 abstract 2
- 241000006271 Discosoma sp. Species 0.000 abstract 1
- 230000004931 aggregating effect Effects 0.000 abstract 1
- 230000003993 interaction Effects 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Hematology (AREA)
- Environmental Sciences (AREA)
- Urology & Nephrology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Plant Pathology (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Claims (20)
1. Нуклеиновая кислота, которая кодирует быстросозревающий хромо- или флуоресцирующий мутант хромопротеина или флуоресцирующего белка книдарии (Cnidaria) или его мутанта.
2. Нуклеиновая кислота по п. 1, где указанная нуклеиновая кислота кодирует хромопротеин или флуоресцирующий белок из небиолюминесцирующих видов книдарии.
3. Нуклеиновая кислота по п. 2, где указанные небиолюминесцирующие виды книдарии относятся к классу Anthozoa.
4. Нуклеиновая кислота по п. 3, где указанный класс Anthozoa относится к Discosoma.
5. Нуклеиновая кислота по п. 4, где указанная нуклеиновая кислота кодирует мутант [белка] DsRed.
6. Нуклеиновая кислота по п. 5, где указанная нуклеиновая кислота кодирует продукт, имеющий точковую мутацию по меньшей мере в одном из положений - положении 2, положении 5, положении 6, положении 21, положении 41, положении 42, положении 44 и положении 117 по сравнению с [белком] DsRed дикого типа.
7. Нуклеиновая кислота по п. 6, где указанный продукт представляет собой продукт, имеющий точковую мутацию по меньшей мере в одном из положений - положении 145 и положении 217.
8. Нуклеиновая кислота по п. 7, где указанная нуклеиновая кислота содержит по меньшей мере 10 остатков последовательностей SEQ ID NO: 01 или 02.
9. Фрагмент нуклеиновой кислоты, выбранный в соответствии с пп. 1-7.
10. Конструкция, содержащая вектор и нуклеиновую кислоту по пп. 1-9.
11. Экспрессирующая кассета, содержащая
(a) область инициации транскрипции, функциональную в экспрессирующем хозяине;
(b) нуклеиновую кислоту по пп. 1-9; и
(c) область терминации транскрипции, функциональную в указанном экспрессирующем хозяине.
12. Клетка или ее предшественник, содержащая экспрессирующую кассету по п. 11 как часть экстрахромосомного элемента или интегрированную в геном хозяйской клетки в результате введения указанной экспрессирующей кассеты в указанную хозяйскую клетку.
13. Способ продуцирования хромопротеина и/или флуоресцирующего белка, где указанный способ включает в себя выращивание клетки по п. 12, в результате чего экспрессируется указанный белок или протеин; и выделение указанного белка или протеина, существенно свободного от других белков или протеинов.
14. Белок или протеин или фрагмент белка или протеина, кодируемый нуклеиновой кислотой по пп. 1-9.
15. Антитело, специфически связывающееся с белком или протеином по п. 14.
16. Трансгенная клетка или ее предшественник, содержащая трансген, который представляет собой нуклеиновую кислоту по пп. 1-9.
17. Трансгенный организм, содержащий трансген, который представляет собой нуклеиновую кислоту по пп. 1-9.
18. Усовершенствование применения, в котором используется хромопротеин или флуоресцирующий белок, где указанное усовершенствование включает в себя применение белка или протеина по п. 14.
19. Усовершенствование применения, в котором используется нуклеиновая кислота, кодирующая хромопротеин или флуоресцирующий белок, где указанное усовершенствование включает в себя применение нуклеиновой кислоты по пп. 1-9.
20. Набор, содержащий нуклеиновую кислоту по пп. 1-9.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US34172301P | 2001-12-19 | 2001-12-19 | |
| US60/341,723 | 2001-12-19 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2004118303A true RU2004118303A (ru) | 2005-03-27 |
| RU2330067C2 RU2330067C2 (ru) | 2008-07-27 |
Family
ID=23338744
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2004118303/13A RU2330067C2 (ru) | 2001-12-19 | 2002-12-18 | Полинуклеотид, кодирующий хромо- или флуоресцирующий мутантный полипептид, вектор экспрессии, клетка, способ получения хромо- или флуоресцирующего полипептида, набор для получения хромо- или флуоресцирующего полипептида, применение полипептида и применение полинуклеотида |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US8664471B2 (ru) |
| EP (1) | EP1456223B1 (ru) |
| JP (2) | JP4700281B2 (ru) |
| AT (1) | ATE439368T1 (ru) |
| AU (1) | AU2002357322A1 (ru) |
| CA (1) | CA2467383C (ru) |
| DE (1) | DE60233347D1 (ru) |
| ES (1) | ES2329666T3 (ru) |
| RU (1) | RU2330067C2 (ru) |
| WO (1) | WO2003054158A2 (ru) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003054158A2 (en) * | 2001-12-19 | 2003-07-03 | The University Of Chicago | Rapidly maturing fluorescent proteins and methods for using the same |
| US20050112685A1 (en) * | 2003-11-26 | 2005-05-26 | Amiss Terry J. | Compositions and methods for measuring analyte concentrations |
| JP4755174B2 (ja) * | 2004-04-07 | 2011-08-24 | ザ ユニヴァーシティー オヴ シカゴ | 単量体赤色蛍光タンパク質 |
| ES2353081T3 (es) * | 2006-01-25 | 2011-02-25 | Evrogen Ip | Nuevas proteínas fluorescentes y métodos para usarlas. |
| US8679749B2 (en) * | 2007-11-01 | 2014-03-25 | The University Of Chicago | Red fluorescent proteins with enhanced bacterial expression, increased brightness and reduced aggregation |
| WO2026004362A1 (ja) * | 2024-06-27 | 2026-01-02 | 浜松ホトニクス株式会社 | 色素タンパク質で標識された哺乳類細胞の製造方法、及び色素タンパク質のスクリーニング方法 |
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-
2002
- 2002-12-18 WO PCT/US2002/040539 patent/WO2003054158A2/en not_active Ceased
- 2002-12-18 EP EP02805620A patent/EP1456223B1/en not_active Expired - Lifetime
- 2002-12-18 ES ES02805620T patent/ES2329666T3/es not_active Expired - Lifetime
- 2002-12-18 RU RU2004118303/13A patent/RU2330067C2/ru active
- 2002-12-18 CA CA2467383A patent/CA2467383C/en not_active Expired - Lifetime
- 2002-12-18 AT AT02805620T patent/ATE439368T1/de not_active IP Right Cessation
- 2002-12-18 AU AU2002357322A patent/AU2002357322A1/en not_active Abandoned
- 2002-12-18 DE DE60233347T patent/DE60233347D1/de not_active Expired - Lifetime
- 2002-12-18 JP JP2003554863A patent/JP4700281B2/ja not_active Expired - Lifetime
-
2004
- 2004-05-11 US US10/844,064 patent/US8664471B2/en active Active
-
2010
- 2010-12-14 JP JP2010277642A patent/JP2011092198A/ja active Pending
-
2014
- 2014-01-21 US US14/159,724 patent/US20140237632A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20050149994A1 (en) | 2005-07-07 |
| RU2330067C2 (ru) | 2008-07-27 |
| ES2329666T3 (es) | 2009-11-30 |
| WO2003054158A2 (en) | 2003-07-03 |
| CA2467383A1 (en) | 2003-07-03 |
| ATE439368T1 (de) | 2009-08-15 |
| WO2003054158A3 (en) | 2003-12-04 |
| US20140237632A1 (en) | 2014-08-21 |
| EP1456223A2 (en) | 2004-09-15 |
| EP1456223A4 (en) | 2005-02-09 |
| JP2006501804A (ja) | 2006-01-19 |
| CA2467383C (en) | 2012-08-28 |
| JP4700281B2 (ja) | 2011-06-15 |
| EP1456223B1 (en) | 2009-08-12 |
| JP2011092198A (ja) | 2011-05-12 |
| AU2002357322A1 (en) | 2003-07-09 |
| US8664471B2 (en) | 2014-03-04 |
| DE60233347D1 (de) | 2009-09-24 |
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