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RU2001104998A - The method of obtaining L-amino acids using bacteria belonging to the genus Escherichia - Google Patents

The method of obtaining L-amino acids using bacteria belonging to the genus Escherichia

Info

Publication number
RU2001104998A
RU2001104998A RU2001104998/13A RU2001104998A RU2001104998A RU 2001104998 A RU2001104998 A RU 2001104998A RU 2001104998/13 A RU2001104998/13 A RU 2001104998/13A RU 2001104998 A RU2001104998 A RU 2001104998A RU 2001104998 A RU2001104998 A RU 2001104998A
Authority
RU
Russia
Prior art keywords
amino acid
bacterium
amino acids
bacteria
sequence
Prior art date
Application number
RU2001104998/13A
Other languages
Russian (ru)
Other versions
RU2215782C2 (en
Inventor
Екатерина Александровна Таболина
Константин Вячеславович Рыбак
Евгений Моисеевич Хургес
Original Assignee
Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика"
Filing date
Publication date
Application filed by Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" filed Critical Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика"
Priority claimed from RU2001104998A external-priority patent/RU2215782C2/en
Priority to RU2001104998A priority Critical patent/RU2215782C2/en
Priority to EP04028877A priority patent/EP1526181B1/en
Priority to DE60210697T priority patent/DE60210697T2/en
Priority to BR122013017187A priority patent/BR122013017187B1/en
Priority to CN2009101594504A priority patent/CN101597589B/en
Priority to CN2009101594487A priority patent/CN101597588B/en
Priority to EP02003335A priority patent/EP1239041B1/en
Priority to BRPI0200350-3A priority patent/BR0200350B1/en
Priority to EP04028876A priority patent/EP1526179B9/en
Priority to BR122013017189A priority patent/BR122013017189B1/en
Priority to DE60219969T priority patent/DE60219969T2/en
Priority to US10/073,293 priority patent/US7476531B2/en
Priority to JP2002034760A priority patent/JP5087194B2/en
Priority to CNB021080860A priority patent/CN100529057C/en
Priority to DE60219968T priority patent/DE60219968T2/en
Publication of RU2001104998A publication Critical patent/RU2001104998A/en
Publication of RU2215782C2 publication Critical patent/RU2215782C2/en
Application granted granted Critical
Priority to JP2007273965A priority patent/JP5087362B2/en
Priority to JP2007273972A priority patent/JP2008067714A/en
Priority to US12/120,404 priority patent/US7618803B2/en
Priority to US12/120,409 priority patent/US7618804B2/en

Links

Claims (8)

1. Бактерия - продуцент L-аминокислоты, принадлежащая к роду Escherichia, отличающаяся тем, что продукция L-аминокислоты упомянутой бактерией увеличена за счет повышения активности белков, описанных в пунктах (А) или (В), в клетке упомянутой бактерии: (A) белок, который представлен аминокислотной последовательностью, приведенной в списке последовательностей под номером 3, (B) белок, который представлен аминокислотной последовательностью, включающей делеции, замены, вставки или добавление одной или нескольких аминокислот в аминокислотную последовательность, приведенную в списке последовательностей под номером 3, и который обладает активностью, придающей бактерии устойчивость к L-аминокислотам и/или их аналогам.1. A bacterium is an L-amino acid producer belonging to the genus Escherichia, characterized in that the production of an L-amino acid by said bacterium is increased by increasing the activity of the proteins described in (A) or (B) in the cell of said bacterium: (A) a protein that is represented by the amino acid sequence listed in sequence number 3, (B) a protein that is represented by an amino acid sequence including deletions, substitutions, insertions, or addition of one or more amino acids to the amino acid sequence elnost that described under number 3 in the sequence listing, and which has an activity of conferring resistance to bacteria L-amino acids and / or their analogs. 2. Бактерия по п.1, отличающаяся тем, что упомянутые активности белков, описанных в пунктах (А) или (В), повышены путем трансформации бактерии с помощью ДНК, кодирующей белки, описанные в пунктах (А) или (В), или путем изменения регуляции экспрессии последовательности указанной ДНК в хромосоме упомянутой бактерии.2. The bacterium according to claim 1, characterized in that said activity of the proteins described in points (A) or (B) is enhanced by transforming the bacterium using DNA encoding the proteins described in points (A) or (B), or by changing the regulation of expression of the sequence of said DNA in the chromosome of said bacterium. 3. Бактерия по п.2, отличающаяся тем, что трансформация осуществляется с использованием многокопийного вектора.3. The bacterium according to claim 2, characterized in that the transformation is carried out using a multi-copy vector. 4. Способ получения L-аминокислоты путем выращивания бактерии по любому из пп.1-3 в питательной среде и сбора полученной и накопленной в культуральной жидкости L-аминокислоты.4. A method for producing an L-amino acid by growing a bacterium according to any one of claims 1 to 3 in a nutrient medium and collecting the L-amino acid obtained and accumulated in the culture fluid. 5. Способ по п.4, отличающийся тем, что упомянутой L-аминокислотой является L-треонин.5. The method according to claim 4, characterized in that said L-amino acid is L-threonine. 6. Способ по п.5, отличающийся тем, что у упомянутой бактерии повышена экспрессия треонинового оперона.6. The method according to claim 5, characterized in that said bacteria have increased expression of a threonine operon. 7. Способ по п.4, отличающийся тем, что упомянутой L-аминокислотой является L-валин.7. The method according to claim 4, characterized in that said L-amino acid is L-valine. 8. Способ по п.7, отличающийся тем, что у упомянутой бактерии повышена экспрессия ilv оперона.8. The method according to claim 7, characterized in that the said bacteria have increased expression of the ilv operon.
RU2001104998A 2001-02-13 2001-02-26 Method for preparing l-amino acids, strain escherichia coli as producer of l-amino acid (variants) RU2215782C2 (en)

Priority Applications (19)

Application Number Priority Date Filing Date Title
RU2001104998A RU2215782C2 (en) 2001-02-26 2001-02-26 Method for preparing l-amino acids, strain escherichia coli as producer of l-amino acid (variants)
DE60219969T DE60219969T2 (en) 2001-02-13 2002-02-13 Method for the production of L-amino acids by means of bacteria of the genus Escherichia
JP2002034760A JP5087194B2 (en) 2001-02-13 2002-02-13 Method for producing L-amino acid using Escherichia bacterium
BR122013017187A BR122013017187B1 (en) 2001-02-13 2002-02-13 transgenic l-amino acid producing bacterium belonging to the genus escherichia, and method for producing l-amino acid
CN2009101594504A CN101597589B (en) 2001-02-13 2002-02-13 Method for producing l-amino acid using bacteria belonging to the genus escherichia
CN2009101594487A CN101597588B (en) 2001-02-13 2002-02-13 Method for producing l-amino acid using bacteria belonging to the genus escherichia
EP02003335A EP1239041B1 (en) 2001-02-13 2002-02-13 Method for producing L-amino acid using bacteria belonging to the genus Escherichia
BRPI0200350-3A BR0200350B1 (en) 2001-02-13 2002-02-13 Transgenic bacterium producing L-amino acid belonging to the genus Escherichia and method for producing L-amino acid
EP04028876A EP1526179B9 (en) 2001-02-13 2002-02-13 Method for producing l-amino acid using bacteria belonging to the genus escherichia
BR122013017189A BR122013017189B1 (en) 2001-02-13 2002-02-13 l-amino acid producing bacterium belonging to the genus escherichia, and method for producing l-amino acid
EP04028877A EP1526181B1 (en) 2001-02-13 2002-02-13 Method for producing l-amino acid using bacteria belonging to the genus escherichia
US10/073,293 US7476531B2 (en) 2001-02-13 2002-02-13 Method for producing L-amino acid using bacteria belonging to the genus Escherichia
DE60210697T DE60210697T2 (en) 2001-02-13 2002-02-13 Process for the production of L-amino acids by means of bacteria of the genus Escherichia
CNB021080860A CN100529057C (en) 2001-02-13 2002-02-13 Method for producing L-amino acid by Escherichia bacterium
DE60219968T DE60219968T2 (en) 2001-02-13 2002-02-13 Process for the production of L-amino acids by means of bacteria of the genus Escherichia
JP2007273965A JP5087362B2 (en) 2001-02-13 2007-10-22 Method for producing L-amino acid using Escherichia bacterium
JP2007273972A JP2008067714A (en) 2001-02-13 2007-10-22 Method for producing l-amino acid using bacteria belonging to genus escherichia
US12/120,404 US7618803B2 (en) 2001-02-13 2008-05-14 Method for producing L-amino acid using bacteria belonging to the genus Escherichia
US12/120,409 US7618804B2 (en) 2001-02-13 2008-05-14 Method for producing L-amino acid using bacteria belonging to the genus Escherichia

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2001104998A RU2215782C2 (en) 2001-02-26 2001-02-26 Method for preparing l-amino acids, strain escherichia coli as producer of l-amino acid (variants)

Related Child Applications (1)

Application Number Title Priority Date Filing Date
RU2001117633A Substitution RU2215785C2 (en) 2001-02-13 2001-06-28 Method for preparing l-amino acids, strain escherichia coli as producer of l-amino acid (variants)

Publications (2)

Publication Number Publication Date
RU2001104998A true RU2001104998A (en) 2003-06-10
RU2215782C2 RU2215782C2 (en) 2003-11-10

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Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2261913C1 (en) * 2004-01-15 2005-10-10 Институт биоорганической химии им.академиков М.М. Шемякина и Ю.А. Овчинникова Российской академии наук RECOMBINANT PLASMID DNA pES6-1 ENCODING HUMAN POLYPEPTIDE INTERFERON BETA-1b AND STRAIN Escherichia coli BDEES6 AS PRODUCER OF HUMAN RECOMBINANT INTERFERON BETA-1b
CN101297042A (en) * 2005-10-26 2008-10-29 纳幕尔杜邦公司 Fermentive production of four carbon alcohols
JP2012029565A (en) * 2008-11-27 2012-02-16 Ajinomoto Co Inc Method for producing l-amino acid
RU2009136544A (en) 2009-10-05 2011-04-10 Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО АГРИ) (RU) METHOD FOR PRODUCING L-CISTEINE USING THE ENTEROBACTERIACEAE FAMILY BACTERIA
ES2646165T3 (en) 2009-11-30 2017-12-12 Ajinomoto Co., Inc. Bacteria that produces L-cysteine and procedure for the production of L-cysteine
RU2460793C2 (en) * 2010-01-15 2012-09-10 Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО АГРИ) Method for producing l-amino acids with use of bacteria of enterobacteriaceae family
BR112013006031A2 (en) 2010-09-14 2016-06-07 Ajinomoto Kk bacteria, and method for producing a sulfur-containing amino acid, a related substance, or a mixture thereof.
JP2014087259A (en) 2011-02-22 2014-05-15 Ajinomoto Co Inc L-cysteine-producing bacterium, and production method of l-cysteine
CN104160024A (en) 2011-04-01 2014-11-19 味之素株式会社 Method for producing l-cysteine
RU2013118637A (en) 2013-04-23 2014-10-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ") METHOD FOR PRODUCING L-AMINO ACIDS USING THE BACTERIA OF THE ENTEROBACTERIACEAE FAMILY IN WHICH THE yjjK GENE IS ADJUSTABLE
RU2013144250A (en) 2013-10-02 2015-04-10 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ") METHOD FOR PRODUCING L-AMINO ACIDS USING THE BACTERIA OF THE Enterobacteriaceae FAMILY IN WHICH THE EXPRESSION OF A GENE CODING A PHOSPHATE TRANSPORTER IS DECREASED
EP2886651B1 (en) 2013-10-21 2018-08-22 Ajinomoto Co., Inc. Method for producing l-amino acid
RU2014105547A (en) 2014-02-14 2015-08-20 Адзиномото Ко., Инк. METHOD FOR PRODUCING L-AMINO ACIDS USING THE ENTEROBACTERIACEAE FAMILY HAVING A SUPER EXPRESSED GENE yajL
RU2015120052A (en) 2015-05-28 2016-12-20 Аджиномото Ко., Инк. A method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family in which gshA gene expression is weakened
JP7066977B2 (en) 2017-04-03 2022-05-16 味の素株式会社 Manufacturing method of L-amino acid
WO2020067487A1 (en) 2018-09-28 2020-04-02 Ajinomoto Co., Inc. Method for producing l-methionine using a bacterium
EP3861109A1 (en) 2018-10-05 2021-08-11 Ajinomoto Co., Inc. Method for producing target substance by bacterial fermentation
BR112021014194A2 (en) 2019-02-22 2021-12-28 Ajinomoto Kk Method for producing an l-amino acid
WO2020204179A1 (en) 2019-04-05 2020-10-08 Ajinomoto Co., Inc. Method of producing l-amino acids
JP7655312B2 (en) 2019-09-25 2025-04-02 味の素株式会社 Method for producing L-amino acids by bacterial fermentation

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5017483A (en) * 1986-02-20 1991-05-21 Kyowa Hakko Kogyo Co., Ltd. Process for producing L-threonine
SU1694643A1 (en) * 1987-11-26 1991-11-30 Всесоюзный научно-исследовательский институт генетики и селекции промышленных микроорганизмов Strain of bacteria escherichia coli - a producer of l-threonine
US5705371A (en) * 1990-06-12 1998-01-06 Ajinomoto Co., Inc. Bacterial strain of escherichia coli BKIIM B-3996 as the producer of L-threonine

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