RU2000800C1 - Method for production of licorice root extract - Google Patents

Abstract

Use: in medicine, specifically about the pharmaceutical and pharmaceutical industry to obtain the root extract Sudnost .: crushed wp ji are crushed and go for 30 minutes at 2000 rpm homogonag extraction is carried out with physical RPM &ngr;;amp; in a primitive environment at room temperature, we treat the patient with 4-8 ° C for 18-0 hours, yes / ie the path can withstand P for 1.0-1.5 hours at 37 - J8 ° C and cs otchoy product otdept and mpl-fugir - vanirm at least 0 min npi 8000 g / min. tapp

Description

The invention relates to medicine, pharmacology and immunology, and can be used to prepare preparations used as immunostimulants in weakened immunity.

A number of methods are known for extracting pharmacologically active substances for the preparation of medicaments. Their essence consists in the isolation of shsharizinic acid by precipitation of | Р1МГе% with sulfuric acids on riMi ir i water and water-acetone salts of licorice, followed by purification by crystallization from acetic acid and alcohol,

To prepare preparations based on glycyrrhetinic acid, the glycyrrhizic acid obtained by this method is subjected to acid hydrolysis. The pharmacological activity of preparations of glycyrrhynetinum and glycyrrhizic acid obtained by this method differs significantly from native licorice root extracts. Pharmacopia provides a number of methods for the preparation of drugs from licorice root (complex licorice root powder, licorice root, licorice syrup, root, dry licorice extract, thick licorice extract), but all of them suggest the use of rather stringent conditions for the isolation of extractable compounds, primarily aimed at increasing the yield of glycyrrhizic acid, and do not ensure the preservation of the native properties of the remaining biologically active substances that make up the extract a.

An object of the invention is to provide a method for the preparation of licorice extract having pronounced immunostimulating properties.

This goal is achieved by the fact that conditions are used to obtain licorice extracts that preserve the native properties of biologically active compounds and promote the activation of non-specific immunity factors.

The essence of the invention is to expand the range of uses of medicinal plants for prevention and treatment.

Preparation of an aqueous extract of licorice root was carried out as follows. The root, dried to a constant mass, untreated from the outer cortical layer, was first crushed on a fine metal grater and then homogenized for 20 min at 2000 rpm. The obtained sample homogenate from the calculation

10% of the dry mass was transferred to physiological saline (pH 7.0), the extracting suspension was stirred for 30 minutes at room temperature on a magnetic stirrer, and then placed for 18 hours in a refrigerator (+ 4 ° C). The next day, after 24 hours from the start of extraction, the solution was thermostated at 37 ° C for 1 hour, after which the suspension was centrifuged for 20 minutes at 8000 rpm. The precipitate was discarded, and the supernatant was used in biological experiments.

Analysis of the content of extractive substances, glycyrrhizic acid and other biologically active compounds accompanying them, performed in accordance with the requirements of the state pharmacopoeia and technical conditions for the production of glycyrrhizic acid (GOST 22840-77), showed that 70-80 goes into the extract as a part of the preparation method % biologically active substances contained in the licorice root (see table 1).

To assess the effect of licorice root extracts on cellular Igumoral immunity in rats with an average body weight of 240 g, the preparation was administered intragastrically 4 times at the rate of 2 ml / kg with each administration. The administration scheme is as follows. The first two are daily, one administration per day, then the two-day interval between the next two daily administrations.

Saline was injected with control animals. There were 12 animals in each group. One day after the fourth administration, all rats were simultaneously tested according to the following indicators:

the phagocytic activity of neutrophils, determined by the percentage of phagocytic cells, served as a test microbe E.coll;

serum complement activity for 50% hemolysis;

functional activity of T- and B-lymphocytes, determined by the percentage of digging cells;

the level of natural antibodies to the salmonella antigen of passive hemagglutination reactions, using for this purpose the standard diagnosticum of the All-Russian Research Institute of Epidemiology and Microbiology. T.N.Gabrichevsky.

The research results were subjected to static processing by the Fisher-Student test. Changes in indicators were considered significant at P 0.05. The data obtained are presented in table. 2.

The analysis presented in table. 2 results show that administration of licorice root extracts to animals has

a positive effect on most of the used immunological tests, moreover, occupying the most important place among indicators of nonspecific resistance of the organism.

Example 1.1 kg dried to a constant mass, the root, untreated from the outer cortical layer, is ground first with a fine grater, and then homogenized for 20 min at 2000 rpm.Next, the resulting homogenate is extracted in physiological saline at pH 7.0 in for 30 minutes at room temperature with constant stirring on a magnetic stirrer, then at a temperature of + 4 ° С for 18 hours they were placed in a refrigerator. The resulting solution was placed in a thermostat at a temperature of 37 ° C for 1 h, after which the suspension was centrifuged for at least 20 minutes at 8000 rpm.

PRI me R 2. 1 kg of dried root is ground, as in example 1, homogenized for 30 minutes at 2000 rpm./min., Pol0

5

0

the learned homogenate is extracted with saline at pH 7.0 for 40 min at room temperature with constant stirring on a magnetic stirrer, then at a temperature of + 8 ° C for 20 hours. The solution is placed in a thermostat.: at a temperature of 38 ° C for 1 , 5 hours, after which the suspension is centrifuged for at least 20 minutes at 8000 rpm.

Thus, on the basis of the results obtained, it follows that the administration of the claimed aqueous extract of licorice root has a stimulating effect on factors of nonspecific immunity, which can be used in an unfavorable epidemiological situation for the prevention of bacterial and viral infections.

(56) Licorice root extract. Technical conditions for preparation, GOST 22840-77.

Table 1

i

The quantitative content of extractives and biologically active compounds in licorice root and in extracts of the inventive method of preparation

Substances

Extractive

Glycyrrhizic acid

Flavonoids

Sucrose Ascorbic Acid

The content of extractives and biologically active substances in the licorice root is given according to the data of I. A. Muravyov and Sokolov B.C.

table 2

The effect of the aqueous extract of licorice root on the immune system with four intragastric administration at a dose of 1 ml / kg (analysis one day after the fourth administration)

The content in the claimed extract, containing g in the root of the olodka

33 15 3.5 10 mg%

80%

24

70%

90%

33 mg%

The presence of a statistically significant difference between control and experience.

Claims (1)

The claims METHOD FOR PRODUCING EXTRACT OF ROOT LIQUORICE Naked by treating crushed raw material with an extractant followed by separation of the target product, characterized in that. in order to preserve the native properties of biologically active compounds that contribute to the activation of factors of Nonspecific immunity, crushed 2 0 The raw materials are homogenized for 20-30 minutes at 2000 and the homogenate is extracted with saline in a neutral medium, first at room temperature for 30-40 minutes, then at 4-8 ° C for 18-20 hours, then the extract is kept for 1-1 , 5 hours at 37 - 38 ° C and the target product is separated by centrifugation for at least 20 minutes at 8000.