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PE20020259A1 - Plantas transgenicas que contienen factores enzimaticos moleculares que alteran el contenido proteico en ellas - Google Patents

Plantas transgenicas que contienen factores enzimaticos moleculares que alteran el contenido proteico en ellas

Info

Publication number
PE20020259A1
PE20020259A1 PE2001000858A PE2001000858A PE20020259A1 PE 20020259 A1 PE20020259 A1 PE 20020259A1 PE 2001000858 A PE2001000858 A PE 2001000858A PE 2001000858 A PE2001000858 A PE 2001000858A PE 20020259 A1 PE20020259 A1 PE 20020259A1
Authority
PE
Peru
Prior art keywords
nucleic acid
plant
nicotine
tobacco plant
refers
Prior art date
Application number
PE2001000858A
Other languages
English (en)
Inventor
Mark A Conkling
Yan Li
Original Assignee
Univ North Carolina State
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ North Carolina State filed Critical Univ North Carolina State
Publication of PE20020259A1 publication Critical patent/PE20020259A1/es

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
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    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1077—Pentosyltransferases (2.4.2)

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  • Health & Medical Sciences (AREA)
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  • Organic Chemistry (AREA)
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  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
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  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
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Abstract

SE REFIERE A UNA MOLECULA DE ACIDO NUCLEICO AISLADO COMPUESTA POR EL ELEMENTO QUE ES PRODUCTO DE UN GEN NIC RECEPTOR SELECCIONADO DE: a) ACIDOS NUCLEICOS AISLADOS TENIENDO UNA SECUENCIA SEQ ID NO:1; b) ACIDOS NUCLEICOS AISLADOS QUE HIBRIDIZAN EL COMPLEMENTO DE SEQ ID NO:1 Y SON RECEPTORES DEL ELEMENTO QUE ES PRODUCTO DE UN GEN NIC. EL ACIDO NUCLEICO ES ADN LINEAL, CIRCULAR. SE REFIERE TAMBIEN A UN VECTOR AGROBACTERIUM O LA CELULA DE UNA PLANTA QUE CONTIENEN EL ACIDO NUCLEICO. SE REFIERE ADEMAS A UN METODO PARA OBTENER UNA PLANTA DE TABACO TRANSGENICA CON REDUCCION DEL CONTENIDO DE NICOTINA QUE COMPRENDE: a) INTRODUCIR UN ACIDO NUCLEICO EXOGENO CONTENIENDO EL ELEMENTO QUE ES PRODUCTO DE UN GEN NIC RECEPTOR EN AL MENOS DOS CELULAS DE LA PLANTA DE TABACO PARA PRODUCIR POR LO MENOS UNA CELULA TRANSFORMADA DE LA PLANTA DEL TABACO; b) TOMAR AL MENOS UNA CELULA TRANSFORMADA DE LA PLANTA DE TABACO CONTENIENDO UN ACIDO NUCLEICO EXOGENO EN UN NUMERO SUFICIENTE DE COPIAS PARA REDUCIR EL NIVEL DE NICOTINA DE LA PLANTA DE TABACO REGENERADA Y c) COMPARAR EL NIVEL DE NICOTINA QUE PODRIA PRESENTAR EN LA AUSENCIA DE UN ACIDO NUCLEICO EXOGENO. FINALMENTE CREAR UNA PLANTA ALTERANDO EL CONTENIDO DE UNA PROTEINA QUE ES REGULADA POR UN ELEMENTO DE ACTIVACION CIS SELECCIONADO DE UAS-1 QUE UNE UN COMPUESTO ACTIVADOR EL CUAL AUMENTA LA EXPRESION DE DICHA PROTEINA Y QUE CON POR LO MENOS UNA CELULA TRANSFORMADA DE LA PLANTA CONTIENE EL ACIDO NUCLEICO EXOGENO
PE2001000858A 2000-08-30 2001-09-12 Plantas transgenicas que contienen factores enzimaticos moleculares que alteran el contenido proteico en ellas PE20020259A1 (es)

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Application Number Priority Date Filing Date Title
US22919800P 2000-08-30 2000-08-30

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PE20020259A1 true PE20020259A1 (es) 2002-03-30

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PE2001000858A PE20020259A1 (es) 2000-08-30 2001-09-12 Plantas transgenicas que contienen factores enzimaticos moleculares que alteran el contenido proteico en ellas

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US (8) US7192771B2 (es)
EP (2) EP1313868B1 (es)
JP (1) JP2004507250A (es)
KR (1) KR20030029885A (es)
CN (1) CN1330753C (es)
AR (1) AR030513A1 (es)
AT (1) ATE333506T1 (es)
AU (1) AU2001286843A1 (es)
CA (1) CA2420724A1 (es)
DE (1) DE60121603T2 (es)
EA (1) EA200300317A1 (es)
ES (1) ES2267809T3 (es)
GT (1) GT200100176A (es)
HN (1) HN2001000250A (es)
IL (1) IL154678A0 (es)
PE (1) PE20020259A1 (es)
UY (1) UY26917A1 (es)
WO (1) WO2002018607A2 (es)

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