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LT4001B - Process for preparing transformed prokaryotic host cell, stable gene amplification in the chromosome dna of prokaryotic microorganisms - Google Patents

Process for preparing transformed prokaryotic host cell, stable gene amplification in the chromosome dna of prokaryotic microorganisms Download PDF

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Publication number
LT4001B
LT4001B LTIP1826A LTIP1826A LT4001B LT 4001 B LT4001 B LT 4001B LT IP1826 A LTIP1826 A LT IP1826A LT IP1826 A LTIP1826 A LT IP1826A LT 4001 B LT4001 B LT 4001B
Authority
LT
Lithuania
Prior art keywords
dna
bacillus
strain
recipient
chromosome
Prior art date
Application number
LTIP1826A
Other languages
English (en)
Lithuanian (lt)
Inventor
Christiaan Albertus Vaneekelen
Johannes Cornelis Vanderlaan
Leonardus Johannes S Mulleners
Original Assignee
Gist Brocades Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8197582&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=LT4001(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Gist Brocades Nv filed Critical Gist Brocades Nv
Publication of LTIP1826A publication Critical patent/LTIP1826A/xx
Publication of LT4001B publication Critical patent/LT4001B/lt

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2414Alpha-amylase (3.2.1.1.)
    • C12N9/2417Alpha-amylase (3.2.1.1.) from microbiological source
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
LTIP1826A 1987-02-27 1994-01-28 Process for preparing transformed prokaryotic host cell, stable gene amplification in the chromosome dna of prokaryotic microorganisms LT4001B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP87200356 1987-02-27

Publications (2)

Publication Number Publication Date
LTIP1826A LTIP1826A (en) 1995-08-25
LT4001B true LT4001B (en) 1996-06-25

Family

ID=8197582

Family Applications (1)

Application Number Title Priority Date Filing Date
LTIP1826A LT4001B (en) 1987-02-27 1994-01-28 Process for preparing transformed prokaryotic host cell, stable gene amplification in the chromosome dna of prokaryotic microorganisms

Country Status (20)

Country Link
US (2) US5733723A (pt)
EP (1) EP0284126B1 (pt)
JP (1) JP2637532B2 (pt)
KR (1) KR970000188B1 (pt)
CN (1) CN1049247C (pt)
AU (1) AU620326B2 (pt)
BG (1) BG60920B2 (pt)
BR (1) BR8805646A (pt)
CA (1) CA1327175C (pt)
DE (1) DE3883041T2 (pt)
ES (1) ES2045081T3 (pt)
FI (1) FI104326B1 (pt)
HU (1) HUT50877A (pt)
IE (1) IE61743B1 (pt)
LT (1) LT4001B (pt)
LV (1) LV10791B (pt)
NO (1) NO300382B1 (pt)
PT (1) PT86863B (pt)
RU (1) RU2091487C1 (pt)
WO (1) WO1988006623A1 (pt)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0896062A3 (en) * 1987-02-27 1999-04-07 Genencor International, Inc. Transformation of alkalophilic bacillus strains
HUT50877A (en) * 1987-02-27 1990-03-28 Gist Brocades Nv Process for producing stable gene amplification in chromosomal dna of procaryote microorganisms
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
US6287841B1 (en) 1988-02-11 2001-09-11 Genencor International, Inc. High alkaline serine protease
DE69028890T2 (de) * 1989-08-11 1997-02-27 Gist Brocades Nv Ergiebige Herstellung von Protease-Mutanten
US5695976A (en) * 1989-12-18 1997-12-09 Novo Nordisk A/S Stable integration of DNA in bacterial genomes
JP3046344B2 (ja) * 1990-10-24 2000-05-29 塩野義製薬株式会社 新規プロテアーゼ
EP0672154A1 (en) * 1991-11-14 1995-09-20 Novo Nordisk A/S A PROCESS FOR EXPRESSING GENES IN $i(BACILLUS LICHENIFORMIS)
DE4216246A1 (de) * 1992-05-16 1993-11-18 Solvay Enzymes Gmbh & Co Kg Einfaches Verfahren zur stabilen chromosomalen Genamplifikation
US5395763A (en) * 1992-06-24 1995-03-07 Monsanto Company Chromosomal expression vector
DK153992D0 (da) * 1992-12-22 1992-12-22 Novo Nordisk As Metode
US5861273A (en) * 1993-12-21 1999-01-19 Celtrix Phamraceuticals, Inc. Chromosomal expression of heterologous genes in bacterial cells
US6723550B1 (en) 1997-07-15 2004-04-20 Genencor International, Inc. Proteases from gram-positive organisms
GB9724627D0 (en) 1997-11-20 1998-01-21 Genencor Int Bv Gram positive microorganism formate pathway
GB9727470D0 (en) 1997-12-30 1998-02-25 Genencor Int Bv Proteases from gram positive organisms
US6599731B1 (en) 1997-12-30 2003-07-29 Genencor International, Inc. Proteases from gram positive organisms
US6465186B1 (en) 1997-12-30 2002-10-15 Genecor International, Inc. Proteases from gram positive organisms
US6528255B1 (en) 1997-12-30 2003-03-04 Genencor International, Inc. Proteases from gram positive organisms
US6100063A (en) * 1998-02-12 2000-08-08 Novo Nordisk A/S Procaryotic cell comprising at least two copies of a gene
CN1160448C (zh) * 1998-02-12 2004-08-04 诺维信公司 含有两拷贝以不同方向转录之基因的原核细胞
RU2144565C1 (ru) * 1998-11-12 2000-01-20 Государственный научный центр вирусологии и биотехнологии "Вектор" Рекомбинантная плазмидная днк pq_f35, кодирующая гибридный полипептид f 35, обладающий антигенными и иммуногенными свойствами белка vp 35 вируса марбург, способ ее получения и штамм бактерий escherichia coli - сверхпродуцент рекомбинантного полипептида f 35
US6156514A (en) * 1998-12-03 2000-12-05 Sunol Molecular Corporation Methods for making recombinant cells
US6544792B1 (en) 1999-12-21 2003-04-08 Genencor International, Inc. Production of secreted polypeptides
WO2002000907A1 (en) * 2000-06-23 2002-01-03 Novozymes A/S Method for stable chromosomal multi-copy integration of genes
JP5244317B2 (ja) 2003-11-19 2013-07-24 ジェネンコー・インターナショナル・インク セリンプロテアーゼ、セリン酵素をコードする核酸、およびこれらを取り込んだベクターおよび宿主細胞
US7985569B2 (en) 2003-11-19 2011-07-26 Danisco Us Inc. Cellulomonas 69B4 serine protease variants
US20050227356A1 (en) * 2004-04-07 2005-10-13 Lessard Philip A Novel compositions and methods for genetic manipulation of Rhodococcus bacteria
WO2006065272A2 (en) * 2004-05-21 2006-06-22 Idaho Research Foundation, Inc. Methods for altering acetic acid production and enhancing cell death in bacteria
DK1766002T3 (en) * 2004-06-21 2015-12-07 Novozymes As Stable maintenance of multiple copies of the at least two ORF in the same orientation
CN101061214B (zh) 2004-10-22 2012-12-05 诺维信公司 多个多核苷酸拷贝的稳定基因组整合
DE102007021001A1 (de) 2007-05-04 2008-11-06 Ab Enzymes Gmbh Expressionssystem zur antibiotikafreien Produktion von Polypeptiden
CN103290048B (zh) * 2013-06-27 2015-07-15 天津科技大学 一种适用于芽孢杆菌属同源重组的质粒及工程菌
ES2855992T3 (es) 2015-12-11 2021-09-27 Wacker Chemie Ag Cepa de microorganismos y procedimiento para la producción fermentativa exenta de antibióticos de sustancias y proteínas de bajo peso molecular
CN114032189B (zh) * 2021-05-10 2024-02-06 盐城师范学院 一种降解木质素的融合子菌株r3及其应用
GB202304540D0 (en) 2023-03-28 2023-05-10 Univ Tartu Method of biosynthesis

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0134048A1 (en) 1983-07-06 1985-03-13 Gist-Brocades N.V. Molecular cloning and expression in industrial microorganism species

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US30602A (en) * 1860-11-06 Improvement in revolver fire-arms
GB1519148A (en) * 1974-11-19 1978-07-26 Gist Brocades Nv Compositions of matter
NO159863C (no) * 1980-01-07 1989-02-15 Univ Rochester Fremgangsm te for fremstilling og seleksjon av en rant bakteriofag som inneholder et genetisk fragment og koder for alfa-amylase, egnet for bruk i heterolog transformering av en bacillus-verts-mikroorganisme.
IL61982A (en) * 1980-02-15 1984-01-31 Cpc International Inc Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes
EP0074553A3 (en) * 1981-09-11 1985-01-09 The University Of Rochester Method of increasing the yield of a product by altering a microorganism
US4617266A (en) * 1983-04-28 1986-10-14 Genex Corporation Production of Protein A
JPS59205983A (ja) * 1983-04-28 1984-11-21 ジエネツクス・コ−ポレイシヨン 異種遺伝子を原核微生物で発現させる方法
IE81141B1 (en) * 1983-06-24 2000-04-05 Genencor Int Procaryotic carbonyl hydrolases
FR2563533B1 (fr) * 1984-04-27 1986-08-22 Centre Nat Rech Scient Procede d'amplification de l'expression d'un gene determine chez bacillus subtilis et souches obtenues
US4828994A (en) * 1984-09-21 1989-05-09 Genex Corporation Bacillus strains with reduced extracellular protease levels
FR2582316B1 (fr) * 1985-05-22 1988-12-02 Centre Nat Rech Scient Vecteurs d'expression de l'a-amylase dans bacillus subtilis, souches obtenues et procede de preparation d'a-amylase
JP2599946B2 (ja) * 1986-01-15 1997-04-16 アムジェン,インコーポレイテツド ズブチリシン類似体
HUT50877A (en) * 1987-02-27 1990-03-28 Gist Brocades Nv Process for producing stable gene amplification in chromosomal dna of procaryote microorganisms

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0134048A1 (en) 1983-07-06 1985-03-13 Gist-Brocades N.V. Molecular cloning and expression in industrial microorganism species

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
ANDERSON RP, ROTH JR.: "Tandem genetic duplications in phage and bacteria.", ANNU REV MICROBIOL., 1977, pages 473 - 505
DEBADO: "Bacilų molekulinė biologija"
DUNCAN CH, WILSON GA, YOUNG FE.: "Mechanism of integrating foreign DNA during transformation of Bacillus subtilis", PROC NATL ACAD SCI U S A., 1978, pages 3664 - 3668, XP001313760
ERIC F. WAWROUSEK ET AL.: "Structure and organization of a cluster of sic tRNA genes in the space between tandem ribosomal RNA gene sets in Bacillus subtilis", J. BIOL. CHEM., 1983, pages 291 - 298
FERRARI E. ET AL.: "Isolation of an Alanine Racemase Gene from Bacillus subtilis and its Use for Plasmid Maintenance in B. subtilis", NATURE BIOTECHNOLOGY, 1985, pages 1003 - 1007
GAY P ET AL.: "Cloning structural gene sacB, which codes for exoenzyme levansucrase of Bacillus subtilis: expression of the gene in Escherichia coli.", J. BACTERIOL., 1983, pages 1424 - 1431, XP001297951
JANNIÈRE L ET AL.: "Stable gene amplification in the chromosome of Bacillus subtilis.", GENE, 1985, pages 47 - 55, XP023539816, DOI: doi:10.1016/0378-1119(85)90023-X
PALVA I. ET AL.: "Nucleotide sequence of the promoter and NH2-terminal signal peptide region of the alpha-amylase gene from Bacillus amyloliquefaciens.", GENE, 1981, pages 43 - 51, XP023542084, DOI: doi:10.1016/0378-1119(81)90103-7
YOUNG M.: "Gene amplification in Bacillus subtilis", J GEN MICROBIOL., 1984, pages 1613 - 1621, XP001206294

Also Published As

Publication number Publication date
LTIP1826A (en) 1995-08-25
US6124097A (en) 2000-09-26
CA1327175C (en) 1994-02-22
PT86863B (pt) 1992-05-29
HUT50877A (en) 1990-03-28
FI884903A7 (fi) 1988-10-24
AU620326B2 (en) 1992-02-20
KR890700664A (ko) 1989-04-26
FI104326B (fi) 1999-12-31
ES2045081T3 (es) 1994-01-16
LV10791B (en) 1995-12-20
CN1030787A (zh) 1989-02-01
RU2091487C1 (ru) 1997-09-27
WO1988006623A1 (en) 1988-09-07
FI884903A0 (fi) 1988-10-24
EP0284126B1 (en) 1993-08-11
BR8805646A (pt) 1989-10-17
AU1398188A (en) 1988-09-26
PT86863A (pt) 1988-03-01
KR970000188B1 (en) 1997-01-06
FI104326B1 (fi) 1999-12-31
IE880558L (en) 1988-08-27
DE3883041D1 (de) 1993-09-16
JPH01502398A (ja) 1989-08-24
US5733723A (en) 1998-03-31
LV10791A (lv) 1995-08-20
NO884422L (no) 1988-10-05
JP2637532B2 (ja) 1997-08-06
NO300382B1 (no) 1997-05-20
EP0284126A1 (en) 1988-09-28
CN1049247C (zh) 2000-02-09
IE61743B1 (en) 1994-11-30
BG60920B2 (bg) 1996-06-28
NO884422D0 (no) 1988-10-05
DE3883041T2 (de) 1994-01-20

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