JP2019002015A - Enzyme solution and preparation method thereof - Google Patents

Abstract

A stable enzyme solution for cleaning is provided. Disclosed is an enzyme solution comprising a nonionic surfactant, an enzyme, a room temperature enzyme stabilizer, isothiazolinone, benzisothiazolinone, sodium benzoate, a solubilizer, a pH adjuster, and water. In some embodiments, the enzyme solution also contains a chelating agent. The enzyme solution according to the present disclosure can be used for cleaning medical devices and has good compatibility with nonwovens containing viscose fibers and terylene fibers. The enzyme solution dispersed in the nonwoven fabric not only has good enzyme stability but also good antifungal and antibacterial properties. Furthermore, the enzyme-containing nonwoven fabric according to the present disclosure is less likely to cause damage to the medical device and less likely to corrode metal or rubber parts of the medical device. [Selection figure] None

Description

CROSS REFERENCE TO RELATED APPLICATIONS This application claims priority from Chinese Patent Application No. 2015010023223.4 filed Jan. 16, 2015, the entire disclosure of which is incorporated herein by reference. .

  The present disclosure relates to the field of rinsing and sterilization, and in particular to enzyme solutions, enzyme-containing nonwovens, and methods for their preparation and use.

  Rinsing and sterilization (or disinfection) represent the core steps in processing after use of a medical device. For heavily contaminated medical devices, pre-rinsing or pre-cleaning steps can greatly increase the success rate of subsequent rinsing and sterilization, extend the useful life of the medical device, and reduce the risk of contamination . For example, in a hospital, a doctor removes an endoscope from a patient's body and immediately wipes contaminants on the external surface of the endoscope using a wet tissue or moist gauze containing a rinse solution. The pre-cleaning is finished, and then the endoscope is removed from the light source or the video processor.

  Currently, most hospitals perform pre-cleaning of endoscopes using conventional gauze soaked in an enzyme dilution solution. However, the enzyme in the enzyme dilution solution is relatively poor in stability and may reduce the success rate of pre-washing. Furthermore, conventional gauze is relatively poorly compatible with enzyme solutions. The activity of the enzyme quickly decreases after the enzyme solution is dispersed in conventional gauze, which can also affect the success rate of the prewash.

  It should be understood that various other embodiments can be devised and modified by those skilled in the art in light of the teachings herein without departing from the scope and spirit of the present disclosure. The following detailed description is, therefore, not to be taken in a limiting sense.

  Unless otherwise explained, all numbers representing features, sizes, amounts, and physicochemical properties used in the specification and claims are, in all cases, modified by the term “about”. Should be understood. Therefore, unless stated to the contrary, the numerical parameters set forth in the preceding specification and the appended claims are approximate, and one skilled in the art can utilize the teachings disclosed herein to It can be suitably changed according to the desired characteristics to be obtained. A detailed description of a range of numbers by an endpoint includes all numbers within that range and within any range within that range. For example, 1-5 include 1, 1.1, 1.3, 1.5, 2, 2.75, 3, 3.80, 4, and 5, etc.

Enzyme Solution In some embodiments, the disclosure provides 0.1 to 10 wt% nonionic surfactant, 0.01 to 1.2 wt% enzyme, and 0, based on the total weight of the enzyme solution. 0.03 to 15% by weight of normal temperature enzyme stabilizer, 0.75 to 6% by weight of isothiazolinone, 0.1 to 1% by weight of benzoisothiazolinone, and 0.1 to 1% by weight of sodium benzoate 6.9-10 wt% solubilizer, 0.15-0.5 wt% pH adjuster, and 60-85 wt% water, and an enzyme solution having a pH value of 6-10 ,I will provide a. In some embodiments of the present disclosure, the enzyme solution comprises 0.1-0.7% by weight sodium benzoate. In some embodiments of the present disclosure, the enzyme solution comprises 0.1-0.5% by weight sodium benzoate. In some embodiments of the present disclosure, the enzyme solution comprises about 5-13 wt% cold enzyme stabilizer. In some embodiments of the present disclosure, the enzyme solution comprises about 10-11% by weight of a room temperature enzyme stabilizer.

Nonionic Surfactant In the enzyme solution according to the present disclosure, the nonionic surfactant helps to reduce the surface tension of the enzyme solution and to increase the wetting and cleaning ability of the enzyme solution.

  A number of suitable nonionic surfactants can be used in the enzyme solution of the present disclosure. According to certain embodiments, the nonionic surfactant comprises (i) a polymer derived from at least one component of ethylene oxide, propylene oxide, and butylene oxide, (ii) an alkyl glucoside, (iii) an aliphatic alcohol. Polyoxyethylene ether, and (iv) at least one component selected from the group consisting of ammonium oxide.

  According to a particular embodiment, the polymer derived from at least one component of ethylene oxide, propylene oxide, and butylene oxide is selected from the group consisting of ethylene oxide / butylene oxide copolymers and ethylene oxide / propylene oxide / butylene oxide copolymers. At least one component. According to certain embodiments, EH-6 commercially available from The Dow Chemical Company can be selected as a polymer derived from at least one of ethylene oxide, propylene oxide, and butylene oxide.

  According to certain embodiments, the alkyl glucoside has a molecular weight of 320-350. According to a particular embodiment, the alkyl glucoside comprises at least one component selected from the group consisting of decyl glucoside, octyl glucoside, and dodecyl glucoside. According to certain embodiments, Glucopon 425N / HH commercially available from BASF Corporation can be selected as the alkyl glucoside.

According to a particular embodiment, aliphatic alcohols polyoxyethylene ethers _have the general formula _{RO (CH 2 CH 2 O)} n H ( wherein, n = 8 to 16). According to certain embodiments, the aliphatic alcohol polyoxyethylene ether is a C7-C9 aliphatic alcohol polyoxyethylene ether, a C12-C16 aliphatic alcohol polyoxyethylene ether, and a C9-C11 aliphatic alcohol polyoxyethylene ether. At least one component selected from the group consisting of ethylene ether. According to certain embodiments, A1058 commercially available from Harcros Corporation can be selected as the aliphatic alcohol polyoxyethylene ether.

  The amount of nonionic surfactant is 0.1 to 10% by weight based on the total weight of the enzyme solution. If the amount of the nonionic surfactant is less than 0.1% by weight, the cleaning ability of the enzyme solution may be reduced. If the amount of the nonionic surfactant is more than 10% by weight, the enzyme solution May be adversely affected by excessive foaming, resulting in a decrease in the cleaning ability of the enzyme solution.

Enzymes In the enzyme solution according to the present disclosure, the enzymes help to break down proteins, carbohydrates, fats, and cellulose in contaminants attached to medical devices.

  According to certain embodiments, the enzyme comprises at least one component selected from the group consisting of proteinases, amylases, lipases, and cellulases.

  According to certain embodiments, a commercially available Polarzyme can be selected as a proteinase from Alcalase, Esperase, Everlase, Savinase 16XL, Liquanase, or Novozymes Corporation. According to certain embodiments, Properase commercially available from Genencor Corporation can also be selected as a proteinase.

  According to certain embodiments, Stainzyme, Termamyl 300L, BAN480L, or Duramyl, commercially available from Novozymes Corporation, can be selected as an amylase.

  According to certain embodiments, Lipolase commercially available from Novozymes Corporation or Purafect commercially available from Genencor Corporation can be selected as the lipase.

  According to certain embodiments, Carezyme 4500, or Endolase, commercially available from Novozymes Corporation, can be selected as the cellulase.

  The amount of enzyme is 0.01-1.2% by weight, based on the total weight of the enzyme solution. According to a particular embodiment, the enzyme in the enzyme solution is based on the total weight of the enzyme solution, 0.05-0.6% by weight proteinase, 0.01-0.2% by weight amylase, 01-0.2 wt% lipase and 0.01-0.2 wt% cellulase. If the amount of the enzyme is less than 0.01% by weight, the cleaning ability of the enzyme solution may be reduced. If the amount of the enzyme is more than 1.2% by weight, the stability of the enzyme solution may be reduced. is there.

Room Temperature Enzyme Stabilizer In the enzyme solution according to the present disclosure, the room temperature enzyme stabilizer helps to maintain the activity of the enzyme under normal temperature (10-25 ° C.) conditions.

  A number of room temperature enzyme stabilizers can be suitably used in the enzyme solution according to the present disclosure. In some embodiments, the cold enzyme stabilizer comprises at least one component selected from the group consisting of glycerol, propylene glycol, calcium chloride, magnesium chloride, and boric acid.

  According to certain embodiments, glycerol is commercially available from The Dow Chemical Company.

  According to certain embodiments, propylene glycol, calcium chloride, magnesium chloride, and boric acid are all obtained from Sinopharm Chemical Reagent Co. , Ltd., Ltd. , Commercially available from China.

  The abundance of the room temperature enzyme stabilizer is 0.03 to 15% by weight based on the total weight of the enzyme solution. According to certain embodiments, the room temperature enzyme stabilizer in the enzyme solution is 5-10 wt% glycerol and / or propylene glycol, and 0.03-0.7 wt% chloride based on the total weight of the enzyme solution. Contains calcium and / or magnesium chloride. When the amount of the room temperature enzyme stabilizer is less than 0.03% by weight, the stability of the enzyme in the enzyme solution may be lowered under a room temperature (10 to 25 ° C.) condition. If it is more than% by weight, the cleaning ability of the enzyme solution may be reduced.

Isothiazolinone, benzoisothiazolinone, and sodium benzoate In the enzyme solution according to the present disclosure, isothiazolinone, benzisothiazolinone, and sodium benzoate are used in combination. Inclusion of isothiazolinone, benzoisothiazolinone, and sodium benzoate together in the enzyme solution helps maintain good stability of the enzyme in the enzyme solution under relatively high temperature (above 25 ° C.) conditions. In addition, it contributes to good antifungal and antibacterial properties of the enzyme solution and good compatibility of the enzyme solution with the nonwoven fabric.

  According to a particular embodiment, the isothiazolinone is selected from the group consisting of 5-chloro-2-methyl-4-isothiazolin-3-one (CMI) and 2-methyl-4-isothiazolin-3-one (MI) At least one component. According to certain embodiments, Nipagurad CG commercially available from Clariant Corporation can be selected as isothiazolinone.

  According to a particular embodiment, the benzisothiazolinone comprises 1,2-benzisothiazolin-3-one. According to certain embodiments, BIT (Prexel GXL) commercially available from Lonza Corporation can be selected as the benzoisothiazolinone.

According to certain embodiments, sodium benzoate has a molecular formula of C ₆ H ₅ CO ₂ Na. According to certain embodiments, sodium benzoate is obtained from Shanhai Shantou Guanghua Chemical Reagents Co, Ltd. Are commercially available.

  The enzyme solution is based on the total weight of the enzyme solution, 0.75-6% by weight isothiazolinone, 0.1-1% by weight benzoisothiazolinone, 0.1-1% by weight sodium benzoate, including. If the amount of any one of isothiazolinone, benzoisothiazolinone, and sodium benzoate does not match the above range, the stability of the enzyme in the enzyme solution may decrease under relatively high temperature (above 25 ° C.) conditions The antibacterial and antibacterial properties of the enzyme solution may be reduced, and the compatibility of the enzyme solution with the nonwoven fabric may also be reduced.

  According to certain embodiments, when the weight ratio of isothiazolinone to benzoisothiazolinone is 3: 1 to 30: 1 and the weight ratio of isothiazolinone to sodium benzoate is 1: 1 to 30: 1, The enzyme in the enzyme solution has particularly good stability under relatively high temperature (above 25 ° C.) conditions, and the enzyme solution has particularly good antifungal and antibacterial properties.

Solubilizer In the enzyme solution according to the present disclosure, the solubilizer helps to promote dissolution of the components in the enzyme solution.

  A number of solubilizers are suitably used in the enzyme solution of the present disclosure. According to certain embodiments, the solubilizer comprises at least one component selected from the group consisting of xylene sulfonic acid, sodium xylene sulfonate, xylene sulfate, sodium xylene sulfate, and alkylamine oxide.

  According to certain embodiments, SXS93 commercially available from Kuantum Corporation can be selected as sodium xylene sulfonate.

  The amount of solubilizer present is 6.9-10% by weight, based on the total weight of the enzyme solution. When the amount of the solubilizer is less than 6.9% by weight, some components in the enzyme solution may not be sufficiently dissolved, and when the amount of the solubilizer exceeds 10% by weight, the enzyme solution There is a risk that the activity of the enzyme in the inside may decrease.

pH adjuster In the enzyme solution according to the present disclosure, a pH adjuster is used to adjust the pH value of the enzyme solution.

  Many pH adjusters can be suitably used in the enzyme solution of the present disclosure. According to certain embodiments, the pH adjuster comprises citric acid.

  According to certain embodiments, the citric acid is obtained from Sinopharm Chemical Reagent Co. , Ltd., Ltd. , Commercially available from China.

  The amount of the pH adjuster present is 0.15 to 0.5% by weight based on the total weight of the enzyme solution. If the amount of the pH adjuster is outside the above range, the acidity of the enzyme solution may be too strong (pH value less than 6), or the alkalinity may be too strong (pH value of more than 10). There is a risk of reducing the activity of the enzyme.

  According to certain embodiments, the pH of the enzyme solution is 6-10. According to certain embodiments, the pH of the enzyme solution is 7-9.

Water Water is used as a solvent in the enzyme solution according to the present disclosure.

  According to certain embodiments, the amount of water present is at least 60% by weight, based on the total weight of the enzyme solution. According to certain embodiments, the amount of water present is 85% by weight or less, based on the total weight of the enzyme solution. If the amount of water present is less than 60% by weight, some components in the enzyme solution may not dissolve sufficiently and may lead to undesirable precipitation of some components. If the amount of water present is more than 85% by weight, some components in the enzyme solution may be excessively diluted, which may reduce the cleaning ability of the enzyme solution.

Chelating Agents Enzyme solutions according to the present disclosure can also include 0.2-0.5 wt% chelating agent, based on the total weight of the enzyme solution.

  In the enzyme solution according to the present disclosure, the chelating agent may contain a chelate metal ion, which may be present as an impurity in the enzyme solution, thereby improving the cleaning ability of the enzyme solution.

  A number of chelating agents can be used as chelating agents in the enzyme solutions disclosed herein. According to certain embodiments, the chelating agent comprises at least one component selected from the group consisting of ethylenediaminetetraacetic acid (EDTA) and diethylenetriaminepentaacetic acid.

  According to certain embodiments, Sinopharm Chemical Reagent Co. , Ltd., Ltd. , A commercially available EDTA from China, can be selected as ethylenediaminetetraacetic acid.

  According to a particular embodiment, Dissolvine D50, commercially available from AKZO-Nobel Corporation, can be selected as diethylenetriaminepentaacetic acid.

Corrosion Inhibitors Enzyme solutions according to the present disclosure can also include 0.5-10% by weight of corrosion inhibitors, based on the total weight of the enzyme solution.

  In the enzyme solution according to the present disclosure, the corrosion inhibitor helps to retard or prevent corrosion due to contact with the metal or rubber enzyme solution.

  A number of corrosion inhibitors can be suitably used in the enzyme solution of the present disclosure. According to certain embodiments, the corrosion inhibitor comprises at least one component selected from the group consisting of borate esters, polyethers, and higher alcohols.

  According to certain embodiments, Crodacor BE-LQ- (AP), commercially available from Croda Corporation, can be selected as the borate ester.

Antifoaming agent The enzyme solution according to the present disclosure may also comprise 0.1 to 0.3% by weight of an antifoaming agent, based on the total weight of the enzyme solution.

  In the enzyme solution according to the present disclosure, the antifoaming agent helps to reduce or prevent foaming of the enzyme solution.

  A number of antifoaming agents can be suitably used in the enzyme solution of the present disclosure. According to a particular embodiment, the antifoaming agent comprises at least one component selected from the group consisting of silicone oils, polyethers, and higher alcohols.

  According to a particular embodiment, DK1247 commercially available from the Dow Corning Corporation can be selected as an antifoaming agent.

Method for Preparing Enzyme Solution In some embodiments, the present disclosure provides a method for preparing an enzyme solution comprising mixing components of an enzyme solution according to the present disclosure.

  According to a particular embodiment, the method of preparing the enzyme solution comprises 6.9-10 wt% solubilizer, 0.1-10 wt% nonionic surfactant, 0.75-6 wt% isothiazolinone. 0.1 to 1% by weight of benzoisothiazolinone, 0.1 to 1% by weight of sodium benzoate, and 0.03 to 15% by weight of a normal temperature enzyme stabilizer, successively in 30 to 60% by weight of water. Adding first to 0.15 to 0.5% by weight of a pH adjuster to obtain a second solution having a pH value of 6 to 10 And 0.01 to 1.2% by weight of enzyme and 3 to 51% by weight of water are added to the second solution to obtain an enzyme solution.

  According to a particular embodiment, the method of preparing the enzyme solution comprises 6.9-10 wt% solubilizer, 0.1-10 wt% nonionic surfactant, 0.75-6 wt% isothiazolinone. 0.1 to 1 wt% benzoisothiazolinone, 0.1 to 1 wt% sodium benzoate, 0.03 to 15 wt% room temperature enzyme stabilizer, 0.2 to 0.5 wt% chelate Agent, 0.5-10 wt% corrosion inhibitor, and 0.1-0.3 wt% antifoaming agent are sequentially added to 30-60 wt% water to obtain a first solution. A step of adding 0.15 to 0.5% by weight of a pH adjusting agent into the first solution to obtain a second solution having a pH value of 6 to 10, and 0.01 to 1.2 Adding wt% enzyme and 3 to 51 wt% water into the second solution to obtain an enzyme solution.

  For a detailed discussion of the components of the enzyme solution, see the “Enzyme Solution” section herein.

Enzyme-containing non-woven fabric In some embodiments, the present disclosure is an enzyme-containing non-woven fabric comprising a non-woven fabric and an enzyme solution according to the present disclosure dispersed in the non-woven fabric, wherein the non-woven fabric is 20 to 50 weight based on the total weight of the non-woven fabric. % Of viscose fiber and 50 to 80% by weight of terylene fiber, and an enzyme-containing nonwoven fabric in which the weight ratio of the enzyme solution to the nonwoven fabric is 1.5: 1 to 7: 1.

  For a detailed discussion of enzyme solutions, see the “Enzyme Solutions” section herein.

  Many viscose fibers can be suitably used in the present disclosure. According to a particular embodiment, the viscose fiber is obtained from the following methods: obtaining alkali cellulose by alkalizing natural cellulose, obtaining cellulose xanthate by reaction of alkali cellulose with carbon disulfide, cellulose xanthate To obtain a viscose solution (viscose) by dissolution in a dilute alkaline solution, and to process the viscose solution (viscose) into viscose fibers by a known wet spinning and post-treatment process. be able to.

  A number of terylene fibers can be suitably used in the present disclosure. According to certain embodiments, terylene fibers are esterified or transesterified with the following method: high purity terephthalic acid (PTA) or dimethyl terephthalate (DMT) and ethylene glycol (EG), followed by a polycondensation reaction. To obtain polyethylene terephthalate (PET) and to process polyethylene terephthalate into terylene fibers by known spinning and post-treatment processes.

  If the amount of viscose fiber or terylene fiber does not match the above range, the enzyme in the enzyme solution may be absorbed by the nonwoven fabric, and the compatibility of the enzyme solution with the nonwoven fabric may be reduced.

  When the weight ratio of the enzyme solution to the nonwoven fabric is less than 1.5: 1, the cleaning ability of the enzyme solution in the enzyme-containing nonwoven fabric may be reduced, and the weight ratio of the enzyme solution to the nonwoven fabric is more than 7: 1. In some cases, it may exceed the maximum loading of the enzyme solution in the nonwoven.

Method for Preparing Enzyme-Containing Nonwoven Fabric In some embodiments, the present disclosure is a method for preparing an enzyme-containing non-woven fabric comprising the step of dispersing an enzyme solution according to the present disclosure in a non-woven fabric, wherein the weight ratio of the enzyme solution to the non-woven fabric Provides a method of preparation, wherein from 1.5: 1 to 7: 1.

  For a detailed discussion of enzyme solutions, see the “Enzyme Solutions” section herein.

  For a detailed discussion of nonwovens, see the section “Enzyme-containing nonwovens” herein.

Use of enzyme-containing nonwoven fabrics In some embodiments, the present disclosure provides for the use of enzyme-containing nonwoven fabrics according to the present disclosure in the cleaning of medical devices.

  The enzyme-containing nonwoven fabric according to the present disclosure can be contacted with a medical device for the purpose of cleaning the medical device.

  The following embodiments are intended to illustrate the present disclosure by way of example and not limitation.

  Embodiment 1 is an enzyme solution, based on the total weight of the enzyme solution, 0.1 to 10% by weight of a nonionic surfactant, 0.01 to 1.2% by weight of enzyme, 03-15 wt% ambient temperature enzyme stabilizer, 0.75-6 wt% isothiazolinone, 0.1-1 wt% benzoisothiazolinone, 0.1-1 wt% sodium benzoate, 6.9-10 wt% solubilizer, 0.15-0.5 wt% pH adjuster, 60-85 wt% water, and an enzyme solution having a pH value of 6-10 is there.

  Embodiment 2 is that the nonionic surfactant is (i) a polymer derived from at least one of ethylene oxide, propylene oxide, and butylene oxide, (ii) alkyl glucoside, (iii) aliphatic alcohol polyoxyethylene The enzyme solution according to embodiment 1, comprising at least one component selected from the group consisting of ether and (iv) ammonium oxide.

  Embodiment 3 is the enzyme solution according to embodiment 1 or 2, wherein the enzyme comprises at least one component selected from the group consisting of proteinase, amylase, lipase, and cellulase.

  In Embodiment 4, the amount of proteinase is 0.05 to 0.3% by weight, the amount of amylase is 0.01 to 0.1% by weight, and the amount of cellulase is 0.01 to 0, based on the total weight of the enzyme solution. It is an enzyme solution of Embodiment 3 which is 0.1 weight%.

  Embodiment 5 isothiazolinone comprises at least one component selected from the group consisting of 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one. The enzyme solution according to any one of Embodiments 1 to 4.

  Embodiment 6 is the enzyme solution according to any one of embodiments 1 to 5, wherein the benzisothiazolinone comprises 1,2-benzisothiazolin-3-one.

  Embodiment 7 is an embodiment wherein the weight ratio of isothiazolinone to benzisothiazolinone is 3: 1 to 30: 1 and the weight ratio of isothiazolinone to sodium benzoate is 1: 1 to 30: 1. 6. The enzyme solution according to any one of 6 above.

  Embodiment 8 is any one of Embodiments 1-7, wherein the room temperature enzyme stabilizer comprises at least one component selected from the group consisting of glycerol, propylene glycol, calcium chloride, magnesium chloride, and boric acid. It is an enzyme solution as described in above.

  In Embodiment 9, the amount of glycerol and / or propylene glycol is 5 to 10% by weight, and the amount of calcium chloride and / or magnesium chloride is 0.03 to 0.7% by weight, based on the total weight of the enzyme solution. The enzyme solution according to Embodiment 8.

  Embodiment 10 is an embodiment in which the solubilizer comprises at least one component selected from the group consisting of xylene sulfonic acid, sodium xylene sulfonate, xylene sulfate, sodium xylene sulfate, and alkylamine oxide. It is an enzyme solution as described in any one of these.

  Embodiment 11 is the enzyme solution according to any one of embodiments 1 to 10, wherein the pH adjuster comprises citric acid.

  Embodiment 12 is the enzyme solution according to any one of embodiments 1-11, further comprising 0.2-0.5 wt% chelating agent, based on the total weight of the enzyme solution.

  Embodiment 13 is the enzyme solution according to embodiment 12, wherein the chelating agent comprises at least one component selected from the group consisting of ethylenediaminetetraacetic acid and diethylenetriaminepentaacetic acid.

  Embodiment 14 is the enzyme solution according to any one of embodiments 1-13, further comprising 0.5-10% by weight of a corrosion inhibitor, based on the total weight of the enzyme solution.

  Embodiment 15 is the enzyme solution according to embodiment 14, wherein the corrosion inhibitor comprises at least one component selected from the group consisting of borate esters, silicates, and polyaspartic acid.

  Embodiment 16 is the enzyme solution according to any one of embodiments 1-15, further comprising 0.1-0.3 wt% antifoaming agent based on the total weight of the enzyme solution.

  Embodiment 17 is the enzyme solution according to embodiment 16, wherein the antifoaming agent comprises at least one component selected from the group consisting of silicone oil, polyether, and higher alcohol.

  Embodiment 18 is an enzyme solution as described in any one of Embodiments 1-17 whose pH value is 7-9.

  Embodiment 19 is a method for preparing an enzyme solution, including a step of mixing the components of the enzyme solution according to any one of Embodiments 1 to 18.

  Embodiment 20 comprises 6.9-10 wt% solubilizer, 0.1-10 wt% nonionic surfactant, 0.75-6 wt% isothiazolinone, 0.1-1 wt% Benzisothiazolinone, 0.1 to 1% by weight sodium benzoate, and 0.03 to 15% by weight room temperature enzyme stabilizer are sequentially added to 30 to 60% by weight water to obtain a first solution. A step of adding 0.15 to 0.5% by weight of a pH adjusting agent to the first solution to obtain a second solution having a pH value of 6 to 10, and 0.01 to 1.2. Adding the weight% enzyme and 3 to 51 weight% water into the second solution to obtain an enzyme solution.

  Embodiment 21 comprises 6.9-10 wt% solubilizer, 0.1-10 wt% nonionic surfactant, 0.75-6 wt% isothiazolinone, 0.1-1 wt% Benzisothiazolinone, 0.1 to 1 wt% sodium benzoate, 0.03 to 15 wt% enzyme stabilizer at room temperature, 0.2 to 0.5 wt% chelating agent, 0.5 to 10 wt% A corrosion inhibitor and 0.1 to 0.3% by weight of an antifoaming agent are sequentially added to 30 to 60% by weight of water to obtain a first solution; Adding 5% by weight of a pH adjusting agent into the first solution to obtain a second solution having a pH value of 6 to 10, 0.01 to 1.2% by weight of enzyme, and 3 to 51 Adding the weight percent water into the second solution to obtain an enzyme solution.

  Embodiment 22 is an enzyme-containing nonwoven fabric comprising the nonwoven fabric and the enzyme solution according to any one of Embodiments 1 to 18 dispersed in the nonwoven fabric, wherein the nonwoven fabric is 20 to 20 based on the total weight of the nonwoven fabric. An enzyme-containing nonwoven fabric comprising 50% by weight viscose fiber and 50-80% by weight terylene fiber, and having a weight ratio of the enzyme solution to the nonwoven fabric of 1.5: 1 to 7: 1.

  Embodiment 23 is a method for preparing an enzyme-containing nonwoven fabric, comprising the step of dispersing the enzyme solution according to any one of Embodiments 1 to 18 in a nonwoven fabric, wherein the nonwoven fabric is based on the total weight of the nonwoven fabric. A preparation method comprising 20 to 50% by weight of viscose fiber and 50 to 80% by weight of terylene fiber, and the weight ratio of the enzyme solution to the nonwoven fabric is 1.5: 1 to 7: 1. is there.

  Embodiment 24 is the use of the enzyme-containing nonwoven fabric described in embodiment 22 in the cleaning of medical equipment.

  The following examples and comparative examples are intended to help the understanding of the present invention, and should not be construed as limiting the scope of the present invention. All parts and percentages are by weight unless otherwise indicated.

The raw materials used in Examples and Comparative Examples of the present invention are shown in Table 1 below.

Preparation of enzyme solution Based on the total weight of the enzyme solution under normal temperature (10-25 ° C) and normal pressure (about 1 atmosphere), add the components of the enzyme solution into a glass or stainless steel container, The components are mixed according to the process to obtain an enzyme solution. The process consists of 6.9-10 wt.% Solubilizer, 0.1-10 wt.% Nonionic surfactant, 0.75-6 wt.% Isothiazolinone, 0.1-1 wt.% Benzo. Isothiazolinone, 0.1 to 1 wt% sodium benzoate, and 0.03 to 15 wt% enzyme stabilizer at room temperature are sequentially added to 30 to 60 wt% water, and the first solution is added. A step of adding 0.15 to 0.5% by weight of a pH adjusting agent to the first solution to obtain a second solution having a pH value of 6 to 10, and 0.01 to 1.2% by weight The enzyme and 3-51% by weight of water are added to the second solution to obtain an enzyme solution.

  Furthermore, the first solution is based on the total weight of the enzyme solution, 0.2-0.5 wt% chelating agent, 0.5-10 wt% corrosion inhibitor, 0.1-0.3 wt%. You may further contain a weight% antifoamer as needed.

Preparation of enzyme-containing non-woven fabric Under normal temperature (10 to 25 ° C.) and normal pressure (about 1 atm), the enzyme solution is placed in the non-woven fabric so that the weight ratio of the enzyme solution to the non-woven fabric is 1.5: 1 to 7: 1. Disperse to obtain an enzyme-containing nonwoven fabric.

  According to certain embodiments, the enzyme solution can be dispersed in the nonwoven fabric by suitable means such as spraying and dipping to obtain an enzyme-containing nonwoven fabric.

  In the present disclosure, the cleaning ability of the enzyme solution is evaluated as in the “determination ability test of enzyme solution”.

  In the present disclosure, the activity of the enzyme in the enzyme-containing nonwoven fabric is evaluated as described in “Enzyme activity test”.

  In the present disclosure, the compatibility of the nonwoven fabric with the enzyme solution in the enzyme-containing nonwoven fabric is evaluated as described in “Compatibility test of enzyme solution with nonwoven fabric”.

  In the present disclosure, the stability of the enzyme in the enzyme-containing nonwoven fabric is evaluated as described in “Enzyme Stability Test”.

  In the present disclosure, the antifungal and antibacterial ability of the enzyme-containing non-woven fabric is evaluated as in the “microbe challenge test”.

  In the present disclosure, the degree of damage to the medical device by the enzyme-containing nonwoven fabric is evaluated as in the “endoscopic damage test”.

  In the present disclosure, the corrosivity of the enzyme solution in the enzyme-containing non-woven fabric is evaluated as in the “corrosion test”.

Detergency test of enzyme solution X-OMAT BT medical X-ray film commercially available from Kodak Corporation (The model number of this X-OMAT BT medical X-ray film is XBT-1, and gelatin is provided on the surface thereof. ) To a test piece having a length of 8.0 ± 1.0 mm and a width of 3.0 ± 1.0 mm.

  Adjust the constant temperature water bath to a specific temperature (37-40 ° C.) and maintain that temperature.

  80 mL of enzyme solution is weighed and tested using a 100 mL beaker.

  The beaker containing the enzyme solution to be tested is placed in a constant temperature water bath and kept warm to bring the system to a stable temperature.

  The test piece is put on the mouth of a beaker, and it is placed in a constant temperature enzyme solution to be tested, and the timing is started.

Observe with the naked eye and record the time t ₁ when the gelatin coating on the specimen begins to dissolve and the time t ₂ when the gelatin coating completely dissolves.
Rinse time t (t = t ₂ −t ₁ ) is calculated.

  If the rinsing time t is less than 8 minutes, it indicates that the rinsing ability of the tested enzyme solution is acceptable.

Enzyme Activity Test Using a commercially available Gallery apparatus from Thermo Fisher Corporation, the test is performed by the standard test method “Proteinase Activity Test in Mixed Samples” defined by Novozymes Corporation.

Tested the compatibility test freshly prepared activity of the enzyme solution with nonwoven enzyme solution, activity is recorded as a _1.

The enzyme solution was mixed with the nonwoven fabric in a weight ratio of 1.5: 1 to 7: 1 to obtain an enzyme-containing nonwoven fabric. After the cloth was left for 2 weeks, the enzyme solution was squeezed out from the cloth and squeezed out. testing the activity of the enzyme in, activity is recorded as a _2.
X ₁ = (a1−a2) / a1 × 100.

When X ₁ <5%, it indicates that the enzyme solution in the enzyme-containing nonwoven fabric has good compatibility with the nonwoven fabric.

Enzyme Stability Tests Enzyme stability tests include "Enzyme stability test under normal temperature (10-25 ° C)" and "Enzyme stability test under relatively high temperature (over 25 ° C)". Including.

Testing the activity of the enzyme at room temperature (10 to 25 ° C.) enzyme solution were stability tested freshly prepared under conditions of enzyme activity, is recorded as a _1.

  The enzyme solution is mixed with the nonwoven fabric at a weight ratio of 1.5: 1 to 7: 1 to obtain an enzyme-containing nonwoven fabric.

  The cloth is allowed to stand for 3 months under the conditions of “25 ° C. and humidity 30 to 80%”.

Squeeze enzyme solution from the fabric, and tested for activity of the enzyme solution was squeezed, activity is recorded as a _3.
And _{X 2 = (a1-a3)} / a1 × 100.

If X ₂ <15%, it indicates that the enzyme solution in the enzyme-containing nonwoven fabric has good enzyme stability under normal temperature (20-25 ° C.) conditions.

Enzyme stability test under relatively high temperature (above 25 ° C.) The enzyme activity in a freshly prepared enzyme solution is tested and the activity is recorded as a ₁ .

  The enzyme solution is mixed with the nonwoven fabric at a weight ratio of 1.5: 1 to 7: 1 to obtain an enzyme-containing nonwoven fabric.

  The cloth is allowed to stand for 2 months under the conditions of “54 ° C. and humidity 75%”.

Squeeze enzyme solution from the fabric, and tested for activity of the enzyme solution was squeezed, activity is recorded as a _4.
X ₃ = (a1−a4) / a1 × 100.

If X ₂ <25%, it indicates that the enzyme solution in the enzyme-containing nonwoven has good enzyme stability under relatively high temperature (above 25 ° C.) conditions.

Microbial challenge test A microbial challenge test is performed by the following method GB-15979-2002.

  50 g of enzyme-containing non-woven fabric is weighed with an electronic balance.

  Suspension of mold (Aspergillus niger, Penicillium citrinum, Glyocradium vilens, Ketomium globosum, and Aureobasidium pullulans) or fungi (E. coli, Staphylococcus aurens, Bacillus megathorium, Pseudomonas fluorescens, and Bacillus subtilis) Select a suspension.

1 mL of the suspension of mold or fungus to be tested is poured into the enzyme solution and stirred homogeneously with a sterilized glass rod to obtain a microbial solution sample having a mold or fungus concentration of about 10 ⁵ to 10 ⁶ / mL.

  The above microbial solution sample is allowed to stand in a constant temperature incubator at a specific temperature for 28 days (for mold, the temperature is 28 ± 1 ° C., for bacteria, the temperature is 36 ± 1 ° C.).

  Every 7 days, mold or fungus counts are performed, or streak culture in a medium is performed. If there is no colony growth, it indicates that the enzyme solution passes the challenge test for mold or fungus, and if there is colony growth, it indicates that the enzyme solution fails the challenge test for mold or fungus. , Indicating that there is a risk of mold or fungal growth.

Endoscope damage test The outer surface of the endoscope is rubbed 100 times back and forth with an enzyme-containing non-woven fabric at “100 N, 10 cm / sec”. If the outer surface of the endoscope does not have a scratch that is evident upon visual inspection after rubbing, it indicates that the enzyme-containing nonwoven fabric is less likely to cause damage to the medical device.

Corrosion test Corrosion test is performed in accordance with the requirements in “Technical Standard for Disinfection 2002” compiled by the Ministry of Health of China.

Polishing the surface of one metal sheet (stainless steel sheet) or rubber sheet (styrene-butadiene rubber), clean washing sheets were weighed and the sheet, and the weight is recorded as a _5.

  A metal sheet or rubber sheet is placed in 200 mL of the enzyme solution to be tested, the sheet is immersed for 72 hours and then the sheet is removed.

Metal sheet or rubber sheet should be washed first with water and then the surface of the sheet is cleaned with a brush or other flexible tool (corrosion products present on the surface of the sheet, if any, should be removed from the sheet) after a.), were weighed sheet, the weight is recorded as a _4.
X ₄ = and (a5-a4).

If X ₄ = 0, it indicates that the enzyme solution does not corrode the metal sheet or rubber sheet and therefore passes the corrosivity test for the metal sheet or rubber sheet.

If X ₄ > 0, it indicates that the enzyme solution fails the corrosivity test on the metal sheet or rubber sheet because it corrodes the metal sheet or rubber sheet.

Examples 1-13 and Comparative Examples 1-7
The components and amounts of the enzyme solution and the enzyme-containing non-woven fabric listed in Table 2a, Table 2b, and Table 2c, respectively (the amounts listed in Table 2a, Table 2b, and Table 2c are all calculated by weight percentage). By the above method.

"Cleaning ability test of enzyme solution", "Compatibility test of enzyme solution with nonwoven fabric", "Enzyme stability test", "Microbial challenge test", "Endoscope damage test", and "Corrosion test" , Respectively. The results are shown in Table 3, Table 4, Table 5, Table 6, Table 7, and Table 8, respectively.

According to Table 2a, Table 2b, Table 2c, and Table 3, the enzyme solution in the enzyme-containing nonwoven fabric provided in Examples 1 to 13 has good cleaning ability.

  From Table 2a, Table 2b, Table 2c, and Table 4, the following can be known.

  Of the enzyme-containing nonwovens provided in Examples 1-13, the nonwovens contain appropriate amounts of viscose and terylene fibers, and the enzyme solution contains 0.75-6 wt% isothiazolinone, 0.1 Contains ˜1 wt% benzisothiazolinone and 0.1-1 wt% sodium benzoate, so that the nonwoven fabric has good enzyme compatibility.

  In the enzyme-containing non-woven fabric provided in Comparative Example 1, the non-woven fabric contains only bamboo fibers, and the enzyme solution contains neither benzoisothiazolinone nor sodium benzoate, so the non-woven fabric is compatible with the enzyme. Is inferior.

  In the enzyme-containing non-woven fabric provided in Comparative Example 2, the non-woven fabric contains only viscose fibers, so that the non-woven fabric has poor compatibility with the enzyme.

In the enzyme-containing non-woven fabric provided in Comparative Example 3, the non-woven fabric contains appropriate amounts of viscose fiber and terylene fiber, but the enzyme solution contains appropriate amounts of isothiazolinone, benzoisothiazolinone, and sodium benzoate. It does not contain, so the nonwoven fabric has poor compatibility with the enzyme.

  From Table 2a, Table 2b, Table 2c, and Table 4, the following can be known.

  In the enzyme-containing non-woven fabric provided in Examples 1 to 13, the enzyme solution contains 0.03 to 15% by weight of a normal-temperature enzyme stabilizer, so that the enzyme solution dispersed in the non-woven fabric has a normal temperature (10 to 25 ° C) under good conditions of enzyme stability.

  In the enzyme-containing non-woven fabric provided in Examples 1-13, the enzyme solution was 0.75-6 wt% isothiazolinone, 0.1-1 wt% benzisothiazolinone, and 0.1-1 wt%. Therefore, an enzyme solution dispersed in a nonwoven fabric has good enzyme stability under relatively high temperature (above 25 ° C.) conditions.

  In the enzyme-containing non-woven fabric provided in Comparative Example 1, the enzyme solution contains 0.3% by weight of isothiazolinone and does not contain benzoisothiazolinone or sodium benzoate. Therefore, the enzyme solution dispersed in the non-woven fabric is Have insufficient enzyme stability under relatively high temperature (> 25 ° C.) conditions.

  In the enzyme-containing non-woven fabric provided in Comparative Example 4, the enzyme solution contains 0.5 wt% isothiazolinone, 0.1 wt% benzoisothiazolinone, and 0.5 wt% sodium benzoate. However, the amount of isothiazolinone is insufficient, so that the enzyme solution dispersed in the nonwoven fabric has insufficient enzyme stability under relatively high temperature (over 25 ° C.) conditions.

  In the enzyme-containing non-woven fabric provided in Comparative Example 5, the enzyme solution contains 0.5 wt% isothiazolinone, 1 wt% benzoisothiazolinone, and 0.5 wt% sodium benzoate, The amount of isothiazolinone is deficient, so the enzyme solution dispersed in the nonwoven fabric has insufficient enzyme stability under relatively high temperature (> 25 ° C.) conditions.

  In the enzyme-containing nonwoven provided in Comparative Example 6, the enzyme solution contained 1.5 wt% isothiazolinone and 0.1 wt% benzoisothiazolinone and no sodium benzoate, so the nonwoven The enzyme solution dispersed therein has insufficient enzyme stability under relatively high temperature (> 25 ° C.) conditions.

In the enzyme-containing non-woven fabric provided in Comparative Example 7, the enzyme solution contained 1.5 wt% isothiazolinone and 0.5 wt% sodium benzoate and no benzoisothiazolinone, so the non-woven fabric The enzyme solution dispersed therein has insufficient enzyme stability under relatively high temperature (> 25 ° C.) conditions.

  From Table 2a, Table 2b, Table 2c, and Table 6, the following can be known.

  In the enzyme-containing non-woven fabric provided in Examples 1-13, the enzyme solution was 0.75-6 wt% isothiazolinone, 0.1-1 wt% benzisothiazolinone, and 0.1-1 wt%. Therefore, the enzyme solution dispersed in the nonwoven fabric has good antifungal and antibacterial properties.

  In the enzyme-containing non-woven fabric provided in Comparative Example 3, the enzyme solution contains 0.3% by weight of isothiazolinone and does not contain benzoisothiazolinone or sodium benzoate. Therefore, the enzyme solution dispersed in the non-woven fabric is Have insufficient antifungal and antibacterial properties.

  In the enzyme-containing non-woven fabric provided in Comparative Example 4, the enzyme solution contains 0.5 wt% isothiazolinone, 0.1 wt% benzoisothiazolinone, and 0.5 wt% sodium benzoate. However, the amount of isothiazolinone is insufficient, so that the enzyme solution dispersed in the nonwoven fabric has insufficient antifungal properties and antibacterial properties.

  In the enzyme-containing non-woven fabric provided in Comparative Example 5, the enzyme solution contains 0.5 wt% isothiazolinone, 1 wt% benzoisothiazolinone, and 0.5 wt% sodium benzoate, The amount of isothiazolinone is insufficient, so that the enzyme solution dispersed in the nonwoven fabric has insufficient antifungal and antibacterial properties.

  In the enzyme-containing nonwoven provided in Comparative Example 6, the enzyme solution contained 1.5 wt% isothiazolinone and 0.1 wt% benzoisothiazolinone and no sodium benzoate, so the nonwoven The enzyme solution dispersed therein has insufficient antifungal and antibacterial properties.

In the enzyme-containing non-woven fabric provided in Comparative Example 7, the enzyme solution contained 1.5 wt% isothiazolinone and 0.5 wt% sodium benzoate and no benzoisothiazolinone, so the non-woven fabric The enzyme solution dispersed therein has insufficient antifungal and antibacterial properties.

According to Table 2a, Table 2b, and Table 7, the enzyme-containing non-woven fabric provided in Examples 1 to 9 hardly causes damage to medical devices.

  According to Table 2a, Table 2b, Table 2c, and Table 8, the enzyme-containing non-woven fabric provided in Examples 1 to 13 is less likely to cause corrosion to metal or rubber parts of medical devices.

  In summary, the enzyme solution according to the present disclosure has good cleaning ability. The enzyme solution according to the present disclosure also contains appropriate amounts of isothiazolinone, benzisothiazolinone, and sodium benzoate, so that the enzyme solution has good compatibility with nonwovens containing viscose fibers and terylene fibers. Have. The enzyme solution dispersed in the nonwoven fabric not only has good enzyme stability but also good antifungal and antibacterial properties. Furthermore, the enzyme-containing nonwoven fabric according to the present disclosure is less likely to cause damage to the medical device and less likely to cause corrosion to the metal or rubber part of the medical device.

  While the foregoing detailed description includes many specific details for purposes of illustration, any person skilled in the art has claimed many changes, substitutions, and alternatives to the details. It is understood that it is within the scope of the present invention. Therefore, the present disclosure as set forth in the detailed description is set forth without limiting the claimed invention. The full scope of the invention should be determined by the following claims and their appropriate legal equivalents. All cited references are incorporated herein by reference in their entirety.

Claims (21)

An enzyme solution based on the total weight of the enzyme solution,
0.1 to 10% by weight of a nonionic surfactant;
0.01-1.2 wt% enzyme,
0.03 to 15% by weight of a normal temperature enzyme stabilizer;
0.75 to 6 wt% isothiazolinone;
0.1 to 1% by weight of benzisothiazolinone;
0.1 to 1% by weight sodium benzoate;
6.9-10 wt% solubilizer;
0.15 to 0.5% by weight of a pH adjuster;
60-85% by weight water,
An enzyme solution having a pH value of 6 to 10.   The nonionic surfactant comprises (i) a polymer derived from at least one of ethylene oxide, propylene oxide, or butylene oxide, (ii) an alkyl glucoside, (iii) an aliphatic alcohol polyoxyethylene ether, and ( The enzyme solution according to claim 1, comprising at least one component selected from the group consisting of iv) ammonium oxide.   The enzyme solution according to claim 1, wherein the enzyme comprises at least one component selected from the group consisting of proteinase, amylase, lipase, and cellulase.   The enzyme solution according to claim 1, wherein the room temperature enzyme stabilizer comprises at least one component selected from the group consisting of glycerol, propylene glycol, calcium chloride, magnesium chloride, and boric acid.   The isothiazolinone comprises at least one component selected from the group consisting of 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one. 2. The enzyme solution described in 1.   The enzyme solution according to claim 1, wherein the benzoisothiazolinone comprises 1,2-benzisothiazolin-3-one.   The weight ratio of the isothiazolinone and the benzoisothiazolinone is 3: 1 to 30: 1, and the weight ratio of the isothiazolinone and the sodium benzoate is 1: 1 to 30: 1. Enzyme solution.   The enzyme solution according to claim 1, wherein the solubilizer comprises at least one component selected from the group consisting of xylene sulfonic acid, sodium xylene sulfonate, xylene sulfate, sodium xylene sulfate, and alkylamine oxide.   The enzyme solution according to claim 1, wherein the pH adjuster comprises citric acid.   The enzyme solution according to claim 1, further comprising 0.2 to 0.5% by weight of a chelating agent based on the total weight of the enzyme solution.   The enzyme solution according to claim 10, wherein the chelating agent comprises at least one component selected from the group consisting of ethylenediaminetetraacetic acid and diethylenetriaminepentaacetic acid.   The enzyme solution according to claim 1, further comprising 0.5 to 10% by weight of a corrosion inhibitor based on the total weight of the enzyme solution.   The enzyme solution according to claim 12, wherein the corrosion inhibitor comprises at least one component selected from the group consisting of borate esters, silicates, and polyaspartic acid.   The enzyme solution according to claim 1, further comprising 0.1 to 0.3% by weight of an antifoaming agent based on the total weight of the enzyme solution.   The enzyme solution according to claim 14, wherein the antifoaming agent comprises at least one component selected from the group consisting of silicone oil, polyether, and higher alcohol.   The method of preparing an enzyme solution including the process of mixing the component of the enzyme solution as described in any one of Claims 1-15. Based on the total weight of the enzyme solution,
6.9-10 wt% solubilizer, 0.1-10 wt% nonionic surfactant, 0.75-6 wt% isothiazolinone, 0.1-1 wt% benzisothiazolinone, Sequentially adding 0.1 to 1 wt% sodium benzoate and 0.03 to 15 wt% room temperature enzyme stabilizer to 30 to 60 wt% water to obtain a first solution;
Adding 0.15 to 0.5% by weight of a pH adjusting agent to the first solution to obtain a second solution having a pH value of 6 to 10;
Adding 0.01 to 1.2 wt% enzyme and 3 to 51 wt% water to the second solution to obtain the enzyme solution. Based on the total weight of the enzyme solution,
6.9-10 wt% solubilizer, 0.1-10 wt% nonionic surfactant, 0.75-6 wt% isothiazolinone, 0.1-1 wt% benzisothiazolinone, 0.1 to 1 wt% sodium benzoate, 0.03 to 15 wt% enzyme stabilizer at normal temperature, 0.2 to 0.5 wt% chelating agent, 0.5 to 10 wt% corrosion inhibitor, And 0.1 to 0.3% by weight of an antifoam, sequentially added to 30 to 60% by weight of water to obtain a first solution;
Adding 0.15 to 0.5% by weight of a pH adjusting agent to the first solution to obtain a second solution having a pH value of 6 to 10;
Adding 0.01 to 1.2 wt% enzyme and 3 to 51 wt% water to the second solution to obtain the enzyme solution. An enzyme-containing nonwoven fabric comprising a nonwoven fabric and the enzyme solution according to any one of claims 1 to 15 dispersed in the nonwoven fabric, wherein the nonwoven fabric is 20 to 50 based on a total weight of the enzyme solution. An enzyme-containing nonwoven fabric comprising: wt% viscose fiber; and 50-80 wt% terylene fiber; and the weight ratio of the enzyme solution to the nonwoven fabric is 1.5: 1 to 7: 1.   A method for preparing an enzyme-containing nonwoven fabric, comprising the step of dispersing the enzyme solution according to any one of claims 1 to 15 in the nonwoven fabric, wherein the nonwoven fabric is 20 to 20 based on the total weight of the nonwoven fabric. A preparation method comprising 50% by weight of viscose fiber and 50-80% by weight of terylene fiber, and the weight ratio of the enzyme solution to the nonwoven fabric is 1.5: 1 to 7: 1.   Use of the enzyme-containing nonwoven fabric according to claim 19 in the cleaning of medical equipment.