JP2017128540A - Sea grape extract inhibiting allergy, method for preparing the same, and application of the same - Google Patents

Abstract

An active ingredient that suppresses allergies in sea grapes is provided. A sea grape having a weight ratio of 1: 1 to 20 and pure water are pulverized and mixed until uniform to form a slurry sea grape, and the temperature is 20 to 100 ° C., preferably 60 to 90 ° C. Preferably, the mixture is stirred at 85 ° C. for 30 to 120 minutes, preferably 60 to 105 minutes, more preferably 90 minutes, and the liquid obtained by first filtration using 5 μm and 1 μm filters is used as sea grape extract, A sea grape extract obtained by drying a grape extract after subjecting it to a second filtration with a laminated thin film comprising three kinds of thin films of EW, PW and DK. The above-mentioned extract has an excellent allergy-inhibiting effect, itself does not cause allergies, and can be applied to cosmetics and foods. [Selection] Figure 1

Description

The present invention relates to a sea grape extract, particularly a sea grape extract that suppresses allergies and does not cause allergies, a preparation method thereof, and an application thereof.

Allergy is a phenomenon in which the body's immune system abnormally reacts excessively to environmental substances (also called allergens), and allergens that enter the body bind to IgE antibodies. At this time, mast cells are stimulated to release inflammatory substances such as histamine, causing an inflammatory reaction in the body tissue. Symptoms of allergies vary depending on the part of the body from which histamine is released, and include symptoms such as runny nose, tears, itching, dyspnea, and tingling of the skin. In severe cases, death may occur due to shock. When allergies occur in people with allergies, drugs such as antihistamines are usually used to relieve allergic symptoms, but allergic drugs generally have certain side effects, so they can be found in natural plants or plants. Many people are interested in suppressing allergic activity using the extracted allergy-suppressing ingredients.

Sea grapes belong to green algae, and not only contain rich algae-extracted polysaccharides, but also zero fat, low calorie, and their bubble texture is reminiscent of caviar, making it an optimal natural among Japanese and professionals It is regarded as a healthy food and is a high-class ingredient for Japanese cuisine. Sea grape extract has been designated as a legally usable cosmetic ingredient by the World Cosmetic Association, since a large amount of trace elements contained in sea grapes have anti-aging effects.

In the past, most sea grape extraction technology has been extracted with alcohol or other organic solvents, and according to the Taiwan grape number I484966 sea grape extract extraction method, sea grape powder is roughly extracted with alcohol, Furthermore, hexane and ethyl acetate are used to separate the active ingredients, and these active ingredients have an antitumor function. In Korean Patent No. KR101252149, sea grape extract is prepared with water and C1-C4 alcohol, and this extract has the effects of antioxidant, anti-inflammatory and moisturizing. None of the sea grape extract obtained by the conventional technology has the effect of suppressing allergy, and the sea grape extract is extracted with an organic solvent, so a small amount of residual organic solvent causes harm to the human body. Not necessarily.

Most conventional sea grape extraction techniques use organic solvents, and a small amount of residual organic solvents can be harmful to human health, and the chemical polarity differs depending on the solvent, so that the extraction ability also differs. It is an important problem to be solved by the present invention how to extract a sea grape extract having the effect of allergy prevention on the premise that no organic solvent is used.

In light of the deficiencies and shortcomings of the prior art, the present invention provides a natural and safe sea grape extract, which is not extracted with an organic solvent, but rather with water at a specific temperature and time. It is intended to obtain an active ingredient that is extracted and further filtered through a membrane to suppress allergies in sea grapes.

The object of the present invention is to provide a method for extracting sea grape extract,
Step 1: Add pure water to sea grapes and pulverize to make a slurry. At this time, the weight ratio of sea grapes to pure water is 1-20 parts of pure water with respect to sea grapes. To obtain slurry-like sea grapes,
Step 2: Continue stirring the slurry-like sea grapes, stirring temperature is 20-100 ° C, stirring time is 30-120 minutes,
Step 3: Perform the first filtration and obtain the liquid sea grape extract through the first filtration.
Including the procedure.

In order to achieve the above object, the sea grapes in step 1 are raw sea grapes, dry sea grapes, or powdered sea grapes.

In order to achieve the above object, in Step 2, the stirring temperature is 60 to 90 ° C., ideally 85 ° C., and the stirring time is 60 to 105 minutes, ideally 90 minutes.

In order to achieve the above object, the first filtration in Step 3 uses 5 μm and 1 μm filters.

In order to achieve the above-mentioned purpose, after the first filtration, the second filtration is performed, the second filtration is a thin film filtration, and the first layer thin film of the thin film filtration treatment is an EW film manufactured by GE Co., Ltd. The two-layer thin film is a PW film manufactured by GE, and the third-layer thin film is a DK film manufactured by GE.

Another object of the present invention is to obtain a sea grape extract that suppresses allergies, and is obtained by the above-described method for extracting a sea grape extract.

Another object of the present invention is to obtain a composition comprising an effective amount of an allergy-preventing sea grape extract and a cosmetically, foodically or pharmaceutically acceptable excipient. The product is a cosmetic composition, a food composition or a pharmaceutical composition.

In order to achieve the above object, the effective amount of sea grape extract is 1 mg / ml or more, and ideally, the effective amount of sea grape extract is 4 mg / ml.

In order to achieve the above-mentioned purpose, this cosmetic composition exists in the form of cream, multiphase emulsion, anhydrous composition, aqueous dispersion, oil, milk, balsam, foam, lotion, gel, cream gel, hydroalcoholic solution, Hydroglycolic solution, hydrogel, liniment, serum, soap, shampoo, conditioner, serum, ointment, mousse, pomade, powder, bar, pencil, spray, aerosol, capsule, gelatin capsule , Soft capsules, hard capsules, tablets, dragees, granules, chewable tablets, solutions, suspensions, emulsions, syrups, capsular polysaccharides, jellies and gelatins.

To achieve the above objectives, the cosmetic composition may be incorporated into products such as concealer, makeup foundation, makeup removal lotion, makeup removal milk, eye shadow, lipstick, lip protector, lip gloss and powder.

In order to achieve the above-mentioned purpose, the composition of the shampoo includes 10 to 50% surfactant, 0.3 to 2% emollient, 0.01 to 0.1% sea grape extract, 0 to 2% thickener, 0.1 to fragrance 0.1 1%, preservative 0.3-1%, others water.

Effect of sea grape extract on β-hexosaminidase release when cells are stimulated by calcium ionophore A23187 to degranulate and release β-hexosaminidase Allergy suppression effect of sea grape extract Allergy suppression effect of sea grape extract

Unless otherwise defined, technical and scientific terms used in this document shall be interpreted based on recognition shared by those with ordinary skill in the field to which the term belongs.
EXAMPLES Hereinafter, although an Example is given and detailed description of this invention is given, a restriction | limiting should not be added to this invention with a following example.

Example 1
I. Sea grape extract preparation method (I)
Take 80 g of dried sea grape powder and 1600 g of pure water, and use dried sea grape powder from Cowlerpa lentillifera, pulverize and mix until uniform (temperature about 85 ° C, time about 90 minutes) Thereafter, the mixture is filtered through 5 μm and 1 μm filters to obtain a liquid sea grape extract (I), followed by further thin film filtration. The first layer thin film uses GE's EW membrane to remove large impurities with this precision filter (Microfiltration), and the second layer thin film uses GE's PW membrane with this ultrafilter (Ultrafiltration) The GE membrane DK membrane is used for the third layer thin film, the salt is removed with this nanofilter (Nanofiltration), and the membrane is filtered and freeze-dried to obtain sea grape extract (I). The above crickets may be replaced by other types of sea grapes such as Caulerpa taxifolia.

2. The sea grape extract (I) does not use the stimulant (A23187 (-)) for cells that do not cause allergies, and only the sea grape extract (I) and sea grape extract (I) are added to the β-hexosaminidase Observe the release of (Figure 1). When the sea grape extract (I) alone, the sea grape extract (I) alone and the control group are compared, the sea grape extract (I) and the sea grape extract (I) alone The release of hexosaminidase has not increased, indicating that sea grape extract (I) and sea grape extract (I) itself do not cause allergies.

3. Allergy induction experiment When cells stimulated by calcium ionophore A23187 release β-hexosaminidase, an allergic reaction medium, the concentration of β-hexosaminidase is determined by enzyme-linked immunosorbent assay. From the results of the control group of FIG. 1 measured by ELISA, it can be seen that A23187 stimulation induces β-hexosaminidase release.

4. Allergy suppression of sea grape extract (I) According to the results in Figure 2, sea grape extract (I) suppressed the release of β-hexosaminidase induced by A23187 stimulation (A23187 (+) group) When the sea grape extract (I) concentration was 2 mg / ml, β-hexosaminidase release was suppressed by 8%, and when the sea grape extract (I) concentration was 4 mg / ml, β-hexosaminidase release was suppressed by 25%. The inhibitory effect of sea grape extract (I) on β-hexosaminidase release is more prominent. When sea grape extract (I) concentration is 1 mg / ml, it suppresses β-hexosaminidase release by 45% and sea grape extract (I) concentration At 4 mg / ml, β-hexosaminidase release was suppressed by 82% and there was no effect on cell viability (Fig. 1).

5. Application of sea grape extract (I) to cosmetics Heat a certain amount of pure water to 50-60 ° C, add surfactant in order, and stir to mix until uniform. Stop heating, and when the temperature drops to 20 ° C, add sea grape extract (I), orange essential oil, sodium aniseate, mix until uniform, and mousse shampoo containing sea grape extract (I) Get. The blending ratio of mousse shampoo is shown in Table 1.
Table 1. Mixing ratio of mousse shampoo containing sea grape extract (I)

6. Clinical trials of cosmetics containing sea grape extract (I) Mousse shampoo containing sea grape extract (I) to AMA Laboratories Inc., a US Food and Drug Administration (FDA) certified laboratory (See Table 1) for a clinical trial of skin irritation and allergic reactions. This clinical trial is composed of 52 subjects, 50 subjects who have completed the clinical study, the subject's age range is 20-68 years, and sex and race are shown in Table 2.

Table 2, Statistical data on subjects of mousse shampoo trials containing sea grape extract (I)

As a clinical test method, dilute the mousse shampoo shown in Table 1 10 times with water, take 0.2 ml of the diluted mousse shampoo, apply it to a semi-breathing hypoallergenic adhesive bandage, Apply the skin to the right or left side of the center line and instruct the subject not to moisten or apply it to direct sunlight. After 24 hours, the subject will peel off the adhesive bandage at home, By repeating this procedure, a total of 9 times of pasting was performed, and if a poor reaction was observed, the range where red swelling or edema occurred was measured. The results are shown in Table 3, and 50 subjects who completed the clinical trial showed no allergic reaction to the mousse shampoo containing sea grape extract (I). ) Mousse shampoos are not irritating to the skin and indicate that they do not cause allergies.

Table 3. Clinical trial results of mousse shampoo containing sea grape extract (I)
C: White, H: Hispanic, A: Asian, F: Female, M: Male, 0: No response, Dc: Decline

Example 2
Take 1.5 kg of fresh sea grapes and 1.5 kg of pure water, and use sea cucumbers (Caulerpa lentillifera) to grind and mix until uniform (temperature about 85 ° C., time about 30 minutes), then 5 μm and Filtration through a 1 μm filter to obtain a liquid sea grape extract (II), followed by further membrane filtration. The first layer thin film uses GE's EW membrane to remove large impurities with this precision filter (Microfiltration), and the second layer thin film uses GE's PW membrane with this ultrafilter (Ultrafiltration) As a third layer thin film, GE's DK membrane is used, salt is removed with this nanofilter (Nanofiltration), freeze-dried after thin film filtration, and sea grape extract (II) is obtained. The above crickets may be replaced by other types of sea grapes, such as Caulerpa taxifolia

According to the results of Fig. 3, sea grape extract (II) suppressed the release of β-hexosaminidase induced by A23187 stimulation (A23187 (+) group), and sea grape extract (II) concentration of 1mg / ml Sometimes inhibits the release of β-hexosaminidase by 40%, suppresses the release of β-hexosaminidase by 60% when the concentration of sea grape extract (II) is 2 mg / ml, and suppresses the release of β-hexosaminidase by 60% when the concentration of sea grape extract (II) is 4 mg / ml. hexosaminidase release is suppressed by 80%.

Example 3
Take 1.5 kg of fresh sea grapes and 1.5 kg of pure water, and use dry powder of sea grapes from Caulerpa lentillifera, pulverize and mix until uniform (temperature about 25 ° C, time about 60 minutes) Then, after filtering with a filter of 5 μm and 1 μm to obtain a sea grape extract (III), membrane filtration is further performed. The first layer thin film uses GE's EW membrane to remove large impurities with this precision filter (Microfiltration), and the second layer thin film uses GE's PW membrane with this ultrafilter (Ultrafiltration) As a third layer thin film, GE's DK membrane is used, salt is removed with this nanofilter (Nanofiltration), and after freeze filtration, freeze drying is performed to obtain sea grape extract (III). The above crickets may be replaced by other types of sea grapes such as Caulerpa taxifolia.

According to the results of Fig. 3, sea grape extract (III) suppressed the release of β-hexosaminidase induced by A23187 stimulation (A23187 (+) group), and sea grape extract (III) concentration of 1mg / ml When the concentration is 2 mg / ml, β-hexosaminidase release is suppressed by 56%, and when the concentration is 4 mg / ml, β-hexosaminidase release is suppressed by 75%.

Claims (16)

Step 1: Add pure water to sea grapes and pulverize to make a slurry. The weight ratio of sea grapes and pure water at this time is a ratio of 1 to 20 pure water with respect to sea grapes 1. To obtain slurry-like sea grapes,
Step 2: Continuously stirring slurry sea grapes, stirring temperature is 20-100 ° C., stirring time is 30-120 minutes,
Step 3: Perform the first filtration and obtain a liquid sea grape extract through the first filtration.
A method for extracting sea grape extract, characterized in that the procedure is performed as follows. The sea grape extract method according to claim 1, wherein the sea grapes in step 1 are raw sea grapes, dry sea grapes, or powdered sea grapes. The method for extracting a sea grape extract according to claim 1, wherein in step 2, the temperature of stirring is 60 to 90 ° C. The method of extracting sea grape extract according to claim 1, wherein, in step 2, the stirring temperature is 85 ° C. The method for extracting a sea grape extract according to claim 1, wherein in step 2, the stirring time is 60 to 105 minutes. The method of extracting sea grape extract according to claim 1, wherein the stirring time in Step 2 is 90 minutes. The method for extracting a sea grape extract according to claim 1, wherein the first filtration in step 3 is performed using filters of 5 µm and 1 µm. 2. The method for extracting a sea grape extract according to claim 1, wherein in step 3, the second filtration is further performed after the first filtration, and the second filtration is a thin film filtration. The sea grape extract extraction method according to claim 8, wherein the first layer thin film of the thin film filtration treatment is an EW film, the second layer thin film is a PW film, and the third layer thin film is a DK film. The sea grape extract which suppresses allergy obtained by the extraction method of the sea grape extract in any one of Claims 1-9. A cosmetic, food or pharmaceutical composition comprising an effective amount of the allergy-suppressing sea grape extract according to claim 10 and a cosmetically, foodologically or pharmaceutically acceptable excipient. The composition according to claim 11, wherein the effective amount of the sea grape extract is 1 mg / ml or more. 12. The composition of claim 11, wherein the effective amount of sea grape extract is 4 mg / ml. The cosmetic composition which is the composition according to claim 11 is in the form of cream, multiphase emulsion, anhydrous composition, aqueous dispersion, oil, milk, balsam, foam, lotion, gel, cream gel, aqueous alcohol solution , Hydroglycolic solution, hydrogel, liniment, serum, soap, shampoo, conditioner, serum, ointment, mousse, pomade, powder, bar, pencil, spray, aerosol, capsule, gelatin 12. The capsule of claim 11, selected from the group of formulations consisting of capsules, soft capsules, hard capsules, tablets, dragees, granules, chewable tablets, solutions, suspensions, emulsions, syrups, capsular polysaccharides, jelly and gelatin. Composition. A cosmetic composition which is the composition of claim 11 is incorporated into products such as concealer, makeup foundation, makeup removal lotion, makeup removal milk, eye shadow, lipstick, lip protector, lip gloss and powder. The composition according to claim 11. The composition of the shampoo that is the composition according to claim 14 is 10 to 50% surfactant, 0.3 to 2% emollient, 0.01 to 0.1% sea grape extract, thickener 0 Composition according to claim 14, wherein ~ 2%, fragrance 0.1-1%, preservative 0.3-1%, others are water.