JP2016032481A - Method for producing dispensed liquid coating sheet - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a dispensing method which can deal with various composition systems such as a dispersed system and a highly viscous system, and can collectively form uniform thickness to desired regions, thereby can be suitably used, for example, for formation of culture layer of a microorganism culture sheet.SOLUTION: The invention relates to a method for producing a dispensing liquid coated sheet comprising transcriptionally coating the substrate surface with dispensing liquid 35 in which 20-50% of solid component is contained by discharging from a nozzle composing porous nozzle 125 so that the amount of coating is 250 g/m-1050 g/m, when a clearance h between the discharging face of the porous nozzle 125 composed of a plurality of nozzles with a pore size of 0.9-1.6 mm and the substrate surface of a substrate sheet 10 is 0.3-1.0 mm.SELECTED DRAWING: Figure 5

Description

  The present invention relates to a method for producing a dispensing liquid-coated sheet such as a microorganism culture sheet capable of performing a microorganism test quickly and accurately, for example.

  As a method for confirming the presence of microorganisms or measuring the number of microorganisms, there is an agar plate mixing method. In this method, since an agar medium formed in a petri dish sterilized in advance is used as a medium, an autoclave for high-pressure steam sterilization of the agar medium and a laboratory capable of performing microbial inspection aseptically are required. Moreover, skill is required for the operation of the microorganism test from the sampling of the microorganism to the preparation of the sample solution, dispensing, mixing with the medium, culture, and counting. Therefore, development of a microorganism culture sheet having a dried culture layer that can be easily tested for microorganisms without requiring a high degree of skill has been promoted.

  So far, as a sheet-shaped microorganism incubator reported so far, for example, a water-based adhesive composition layer formed on the upper surface of a support and a gelling agent attached to the adhesive composition layer There is an incubator device comprising a cold water-soluble powder containing a cover sheet and a cover sheet covering the cold water-soluble powder (see Patent Document 1). In addition, there is a microorganism incubator in which a circular water-soluble polymer compound layer and a porous matrix layer are laminated on a square pressure-sensitive adhesive sheet, and a square transparent film is disposed on the top (see Patent Document 2).

Japanese Patent No. 3383304 International Publication No. 01/044437

  As a method for forming a dried culture layer in such a microorganism culture sheet, various printing methods such as screen printing, etc. are considered in consideration of the fact that a certain thickness is required for the culture layer in order to properly grow microorganisms. A coating method is used.

  However, if the medium to be applied is an incompatible dispersion system or a medium with a high viscosity is applied, the printing method or coating method may not be suitable, and the smoothness of the application area may be reduced. There is. The thickness and smoothness of the culture layer in the microorganism culture sheet have a great influence on the test results, leading to a decrease in measurement accuracy.

  The present invention has been made in view of the above problems, and is a microorganism culture sheet that is necessary and sufficient for the growth of microorganisms, has a culture layer of uniform thickness, and can perform an accurate microorganism test. An object of the present invention is to provide a manufacturing method that can be manufactured accurately and continuously.

  The present inventors dispense a medium solution containing components necessary for the growth of microorganisms to a predetermined region on the base sheet using a porous nozzle, and at the time of dispensing, the nozzle and the base are dispensed. By using an indirect transfer method that utilizes the clearance between the medium and the surface tension of the culture medium, even if the culture medium is an incompatible dispersion or high viscosity, the thickness and smoothness of the culture layer Has been found to be uniform, and simultaneous dispensing to the coating area is possible, and the present invention has been completed. Specifically, the present invention provides the following.

(1) The separation distance between the discharge surface of the porous nozzle composed of nozzles having a hole diameter of 0.9 mm to 1.6 mm and the substrate surface is set to 0.3 mm to 1.0 mm.
The solid content of 20% to 50% of the dispensing liquid, dispensing the coating amount of the nozzle constituting the porous nozzle ejected so as to ^{250g / m 2 ~1050g / m 2} , to transfer coating to the substrate surface A method for producing a liquid-coated sheet.

(2) The method for producing a dispensing liquid-coated sheet according to claim 1, wherein the viscosity of the dispensing liquid is 100 to 2000 cp.

(3) the coating amount of the nozzle constituting the porous nozzle, the manufacturing method of dispensing liquid coating sheet according to claim 1 or 2 is a ^50g / ^{m 2 ~410g} / m 2 after drying.

(4) The method for producing a dispensed liquid-coated sheet according to claim 3, wherein the thickness after drying is 100 μm to 500 μm.

(5) The dispense liquid-coated sheet according to any one of claims 1 to 4, wherein the dispense liquid-coated sheet is a microorganism culture sheet, and the dispense liquid is a medium liquid for forming a culture layer of the microorganism culture sheet. Method.

  According to the production method of the present invention, for example, when a microorganism culture sheet is produced, it is necessary and sufficient for the growth of microorganisms, and a culture layer having a uniform thickness can be formed. Differences in speed are unlikely to occur, and it is possible to accurately perform microbial tests such as counting the number of colonies.

It is a whole schematic block diagram which shows an example which applied the dispensing apparatus which can be used conveniently for the manufacturing method of this invention to the manufacture example of a microorganism culture sheet. It is the schematic which shows the whole operation | movement of the dispensing part in FIG. It is the schematic which shows operation | movement of the up-and-down dispense part moving means in FIG. It is a figure which shows the discharge hole of the porous nozzle in FIG. It is the schematic which shows the detail of operation | movement of the upper-and-lower dispense part moving means in FIG. It is a top view which shows the manufacturing method of a microorganism culture sheet | seat sequentially. It is the partial perspective top view (A) and sectional drawing (B) of a microorganism culture sheet. It is a figure which shows the relationship between the application pressure (discharge pressure) and the application quantity in an Example.

  Hereinafter, specific embodiments of the present invention will be described in detail with reference to an example of production of a microorganism culture sheet, which is an example of a dispensing liquid-coated sheet in the present invention, but the present invention is not limited to the following embodiments. The present invention is not limited, and can be implemented with appropriate modifications within the scope of the object of the present invention.

<Configuration of dispensing device>
[Overall configuration of dispensing device]
As shown in FIG. 1, the dispensing apparatus 100 is an apparatus for injecting a dispensing liquid 35 serving as a medium for forming the culture layer 30 into a predetermined region on the base sheet 10. The dispensing apparatus 100 includes a dispensing liquid supply unit 110 that supplies the dispensing liquid 35, a dispensing unit 120 that is connected to the dispensing liquid supply unit 110 and has a porous nozzle 125 at the end thereof, and the dispensing unit 120 is connected to the base sheet 10. A Z-direction movable means 130 that can move in the vertical direction (Z direction in FIG. 1) relative to the horizontal plane, and an X that can move the dispensing unit 120 in one direction (X direction in FIG. 1) in the horizontal plane direction of the base sheet 10. The direction movable means 140 and the Y direction movable means 150 capable of moving the dispensing unit 120 in the other direction (Y direction in FIG. 1) in the horizontal direction of the base sheet 10 are provided.

[Dispensing liquid supply means]
The dispense liquid supply means 110 in this embodiment is mainly composed of a hollow cylindrical container main body 111 that can store the dispense liquid 35, and a discharge pressure control system 118 including a valve 117 and a discharge pressure control device 116. Yes.

  In the container main body 111, a stirring blade 113 for stirring the dispensing liquid 35 inside the container is disposed at the bottom of the substantially central portion, and a rotating shaft for rotating the stirring blade 113 is connected to the motor 112. Yes.

  The container main body 111 and the valve 117 are connected via a hollow tube 114 capable of liquid feeding, and the valve 117 and the dispensing unit 120 are connected via a hollow tube 115 capable of liquid feeding similarly.

  As the valve 117 and the discharge pressure control device 116, which are examples of the discharge pressure control system 118, any known one can be used and is not particularly limited. Further, the discharge pressure control system 118 is not necessarily limited to the pressure control, and may be, for example, a flow rate measurement type flow rate control or a capacity measurement type capacity control.

[Dispensing part]
A multi-hole nozzle 125 is disposed at the tip of the dispensing unit 120 to which the other end of the hollow tube 115 is connected. As shown in FIG. 4, the porous nozzle 125 has a circular discharge surface as a whole, and a large number of discharge holes K are formed on the discharge surface. The discharge holes of the nozzles may be arranged at regular intervals or may be arranged partially densely. In the present embodiment, the arrangement density of the discharge holes K of the multi-hole nozzle is partially increased at a location corresponding to the vicinity of the outer periphery of the culture layer 30. According to such an aspect, when the frame layer 20 is formed on the outer periphery of the culture layer 30, the medium solution can be spread without a gap between the frame layer 20 and the culture layer 30.

[X direction / Y direction / Z direction movable means]
The dispense unit 120 is arranged on the Z stage in the XYZ orthogonal space of FIG. 1 and is slidable in the Z direction by the Z direction movable unit 130. This is the upper and lower dispense unit movable unit in the present invention. Equivalent to. Furthermore, in this embodiment, the Z-direction movable means 130 including the dispensing unit 120 itself can be slid in the X direction and the Y direction by the X-direction movable means 140 and the Y-direction movable means 150. As a result, The dispensing unit 120 can move to an arbitrary point on the XYZ space. The movement control means 160 performs this movement control. In this embodiment, not only the Z direction but also the XY direction can be controlled by this movable control means 160. The Z-direction movable means 130, the X-direction movable means 140, the Y-direction movable means 150, and the movement control means 160 can be each a known stage device, and are not particularly limited.

[Frame part forming means]
As shown in step S1 of FIG. 2, which will be described later, a circumferential frame layer 20 made of hot melt resin is previously formed on the base sheet 10 in order to delimit the range of the culture layer 30 of the microorganism culture sheet. The For this reason, in the dispensing apparatus 100, a hot melt gun 200 is mounted separately from the dispensing unit 120. The hot melt gun 200 is movable in the X direction / Y direction / Z direction, like the dispensing unit 120, and hot melt resin (HM in FIG. 2) can be supplied from the outside, and a single discharge at the tip. The nozzle 210 is provided, and a hot melt resin can be applied to any location and shape by a gun control unit (not shown), whereby the circumferential frame layer 20 can be formed. In the present invention, the frame layer 20 may be a frame portion for defining the range of the culture layer 30, that is, a step portion, and the frame layer 20 is not limited to hot melt resin, for example, UV curable resin. There may be. Alternatively, the frame portion may be formed by a sheet having a predetermined thickness, and the range of the culture layer 30 may be a recess.

<Manufacturing method of dispense liquid coating sheet>
[Preparation stage]
Taking a production of a microorganism culture sheet as an example, a method for producing a dispensing liquid coating sheet using the dispensing apparatus 100 will be described. First, for example, a binder, a gelling agent, a nutritional component, a coloring indicator, a selective agent, etc. are dissolved or dispersed in an alcohol solvent to prepare a dispensing liquid 35 and put it into the container body 111. Rotate to prevent settling. This is the preparatory stage.

  The binder has a role of fixing other components such as a gelling agent, a nutritional component, a coloring indicator, and a selective agent to the base material. For example, polyvinylpyrrolidone, polyethylene oxide, hydroxypropylcellulose, polyvinyl alcohol, etc. In particular, polyvinylpyrrolidone is preferable. Since polyvinylpyrrolidone is soluble in an alcohol solvent, the viscosity of the medium solution can be easily adjusted. Moreover, according to polyvinylpyrrolidone, an alcohol-based solvent can be used, so that a dispersed medium solution can be prepared without dissolving the gelling agent. As a result, it is possible to suppress a significant increase in the viscosity of the medium solution by the gelling agent. Furthermore, after the pattern formation, the solvent can be removed without heating at a high temperature. And since polyvinylpyrrolidone has a high film property, it can be formed into a film by incorporating other components such as a gelling agent, a nutritional component, a coloring indicator, a selective agent, etc. Excellent.

  Examples of the gelling agent include carrageenan, guar gum, chitansan gum, locust bean gum, algin, carboxymethylcellulose, hydroxyethylcellulose and the like. These can be used individually or in mixture of 2 or more types. According to these gelling agents, the culture layer is thickened or gelled by dropping the test solution, and an environment suitable for the growth of microorganisms is obtained. And if a culture layer becomes thickened or gelatinized, since adhesiveness with a cover sheet will improve, evaporation of the water | moisture content at the time of culture | cultivation can also be suppressed.

  The nutrient component can be appropriately selected according to the type of microorganism. For example, for the inspection of general viable bacteria, yeast extract / pepton / glucose mixture, meat extract / pepton mixture, peptone / soybean peptone / glucose mixture, etc., and mixtures obtained by adding dipotassium phosphate and / or sodium chloride to these Can be mentioned. Examples of the test for Escherichia coli and coliform group include sodium desoxycholate / peptone / ammonium iron citrate / sodium chloride / dipotassium phosphate / lactose mixture, and peptone / lactose / dipotassium phosphate mixture. Examples of staphylococci include meat extract, peptone, sodium chloride, mannitol, egg yolk mixture, peptone, meat extract, yeast extract, sodium pyruvate, glycine, lithium chloride, tellurite egg yolk mixture, and the like. For vibrio use, yeast extract, peptone, sucrose, sodium thiosulfate, sodium citrate, sodium cholate, ferric citrate, sodium chloride, bovine bile mixture and the like can be mentioned. Examples of enterococci include bovine brain extract, heart extract, peptone, glucose, dipotassium phosphate and sodium nitride. Examples of fungi include peptone / glucose mixture, yeast extract / glucose mixture, potato extract / glucose mixture, and the like. From these, one or more types can be selected and mixed for use according to the type of microorganism to be grown.

  The color indicator is colored by coloring with the reaction with specific substances produced by metabolism of microorganisms that grow during the culture process, recognizing pH changes, reaction with enzymes, etc., so counting the number of colonies is extremely easy There is an effect to. Examples of the color development indicator include tetrazorim salts such as triphenyltetrazolium chloride (hereinafter referred to as TTC), p-tolyltetrazolium red, tetrazolium violet, petetylyltetrazolium blue, neutral red mixtures, phenol red, bromthymol blue, and thymol. PH indicator such as blue mixture.

  In addition, you may contain the selective agent which suppresses the growth of microorganisms other than the detection objective. As such a selective agent, methicillin, ceftamezole, cefixime, ceftazidime, cefsulosin, bacitracin, polymyxin B, rifampicin, novobiocin, colistin, lincomycin, chloramphenicol, tetracycline, streptomycin and other antibiotics, sulfa drugs, nalidixic acid, Synthetic antibacterial agents such as olaquindox, dyes having bacteriostatic or bactericidal activity such as crystal violet, brilliant green, malachite green, methylene blue, surfactants such as Tergitol 7, dodecyl sulfate, lauryl sulfate, selenite, Examples thereof include inorganic salts such as tellurite, sulfite, sodium nitride, lithium chloride, oxalate, and high-concentration sodium chloride.

  The solvent is not particularly limited as long as it can dissolve or disperse the selected components, but an alcohol solvent is preferably used. Alcohol-based solvents do not need to be heated at a high temperature when removing the solvent after pattern formation, as in high-boiling solvents such as water and toluene, so that the components contained in the culture layer are not likely to be thermally decomposed. Since it does not take a long time to remove the solvent, it is preferable in terms of excellent production efficiency. As the alcohol solvent, an alcohol having 1 to 5 carbon atoms is preferable, and examples thereof include methanol, ethanol, isopropyl alcohol and the like. Among these, methanol is most preferable because it has a low boiling point, can be easily removed by drying, and does not inhibit the growth of microorganisms. In addition, the said solvent can be used individually or in mixture of 2 or more types.

  In the present invention, the solid content of the dispensing liquid 35 is 20% to 50%. Thereby, a drop body can be formed after discharge and transferred onto the surface of the substrate, and leveling can be performed thereafter to form a smooth coated surface. If the solid content is less than 20%, the surface tension is insufficient and it is difficult to form a drop, and the coating amount per nozzle varies. Since both transferability and leveling property are lowered, it is not preferable.

  The viscosity of the dispensing liquid 35 is preferably in the range of 100 to 2000 cp (mPa · s), more preferably 100 to 1200 cp, and particularly preferably 150 to 300 cp. If it is less than 100 cp, the surface tension is insufficient and it is difficult to form a drop, and the coating amount per nozzle varies. This is not preferred, and if it exceeds 1200 cp, the viscosity increases and the ejection from the nozzle, transferability, and leveling properties Since both of them are lowered, it is not preferable. In addition, if an example of a viscosity apparatus and measurement conditions is given, it will be a measured value at the time of 20 degreeC / 500 rpm using Viscotester550 by Eihiro Seiki Co., Ltd. which is an E-type viscosity meter.

  One feature of the present invention is that even with such a high-viscosity dispensing liquid 35, a smooth culture layer 30 having no thickness unevenness can be formed by using the production method of the present invention.

[Frame layer formation]
Next, as shown in step S1 of FIG. 2, a base sheet 10 corresponding to the base in the present invention is placed on the XY plane. The application surface of the base material sheet 10 here corresponds to the base material surface in the present invention. At this time, referring also to FIG. 6, the microorganism culture sheet is in the state of FIG. In addition, the upper stage of each process in FIG. 2 is the front view seen from the X direction, and the lower stage is the top view which looked at the base material sheet 10 from the Z direction. Then, by moving any one or more of the X-direction operation means / Y-direction operation means / Z-direction operation means (not shown) of the hot melt gun 200, the hot melt gun 200 is arranged at a desired position, For example, the circumferential frame layer 20 is formed by rotating the nozzle 210 while discharging hot melt under the control of the gun controller. The microorganism culture sheet is in the state shown in FIG.

  2, after the formation of the one frame layer 20, the X-direction operation means and / or the Y-direction operation means of the hot melt gun 200 is moved so that XY on the base sheet 10 is obtained. A plurality of frame layers 20 may be formed in advance by changing the coordinate position.

[Dispensing process]
Next, by moving one or both of the X-direction movable unit 140 and the Y-direction movable unit 150 of the dispensing apparatus 100, the desired XY coordinate position of the frame layer 20 and the XY coordinate position of the porous nozzle 125 overlap each other. Adjust to. At this time, the position of the dispense portion 120 in the Z direction is sufficiently separated from the base material sheet 10 (the state shown in FIG. 3A).

  At this time, the frame layer 20 on the base sheet 10 and the discharge surface of the porous nozzle 125 are arranged to face each other, and the frame layer 20 on the base sheet 10 as shown in FIG. The recessed area surrounded by the virtual area is divided into a plurality of divided areas P, and the porous nozzles 125 having the discharge holes K assigned to the divided areas P, in other words, the same number of discharges as the divided areas. A multi-hole nozzle 125 having a hole K is disposed.

  In the present invention, the diameter of each discharge hole K of the porous nozzle is preferably 0.1 to 3.0 mm / piece, more preferably 0.3 to 2.0 mm / piece, 0.5 to 2.0 mm / piece is particularly preferred, and 0.9 mm to 1.6 mm / piece is most preferred. If it is the said range, it will be hard to produce the sag and clogging of a culture solution. In particular, in the case of a dispersion medium solution, if the hole exceeds 3.0 mm, liquid sag and unevenness after application are likely to occur, and transferability to the substrate surface and leveling properties are reduced. If it is less than 0.1 mm, clogging is likely to occur depending on the particle size of the dispersed nutrients and the like, and the drop body becomes large, so that the separation distance becomes long, and the transferability also decreases.

The individual hole diameters do not have to be the same size as long as they are within the above range. It arrangement density of the discharge opening K of the porous nozzle is preferably 5 to 20 / cm ^2, more preferably from 5 to 16 pieces / cm ^2, is 8 to 16 / cm ² Is most preferred. When the arrangement density is less than 5 pieces / cm ² , the medium solution does not spread sufficiently, and it may be difficult to form a culture layer having a uniform thickness or a culture layer having a thickness. When the arrangement density exceeds 20 pieces / cm ² , the gap between the holes becomes narrow, so that the medium solutions may come into contact with each other at the time of dispensing and cannot be dispensed satisfactorily. In addition, as a perforated nozzle, what is generally marketed, such as the multi-nozzle MN series made by Musashi Engineering, can be used, for example.

  In this state, as shown in FIG. 3 (b), the Z-direction movable means 130 is controlled to move the dispensing part 120 downward, and as shown in FIG. And stop at a point where the distance to becomes h. The separation distance h is set in advance according to the viscosity of the dispensing liquid 35, the nozzle diameter, and the like. In the present invention, the separation distance h is 0.3 mm to 1.0 mm, preferably 0.8 mm to 1.0 mm. If the thickness is less than 0.3 mm, a sufficient drop cannot be formed, so that a stable coating amount cannot be obtained, and transferability to the substrate surface and leveling properties are deteriorated. Further, since the nozzle itself is contaminated with the dispensing liquid, it is difficult to operate for a long time. When the thickness exceeds 1.0 mm, the dropping body does not reach the substrate surface, and transfer becomes difficult.

Next, the discharge control means 115 is operated, and discharge is started from the porous nozzle 125 as shown in FIG. Here, as shown in the examples described later, the discharge pressure depends on the discharge amount (application amount) from the nozzle, for example, 0.053 MPa to 0.058 MPa, preferably 0.054 MPa to 0.057 MPa. In proportion to this pressure, 140 g / m ² (corresponding to a coating amount of 0.29 g · dry in FIG. 8) to 205 g / m ² (corresponding to a coating amount of 0.40 g · dry in FIG. 8), preferably 155 g / m ² . From m ² (corresponding to a coating amount of 0.31 g · dry in FIG. 8) to 190 g / m ² (corresponding to a coating amount of 0.37 g · dry in FIG. 8) (when dried).

  At the stage shown in FIG. 5B, the discharge amount from the discharge hole K is smaller than the final discharge amount, and the surface tension causes a teardrop-shaped drop body 35b that hangs down from the nozzle tip under its own weight. Thereafter, by continuing the discharge, the dropping body 35c grows to the state of FIG. Around this time, the discharge amount from the discharge hole K becomes close to the final discharge amount. When the discharge is further continued, the dropping body 35d comes into contact with the base sheet 10 as shown in FIG. 5D, and the dropping body moves to the base sheet 10 side due to the wettability with the base sheet 10. It will be transcribed.

The discharge amount from the discharge hole K, that is, the coating amount from one nozzle on the substrate sheet 10 is 0.5 g to 2.0 g (corresponding to 250 to 1050 g / m ² ) before drying. If the amount is less than 0.5 g, a sufficient drop cannot be formed, so that a stable coating amount cannot be obtained, and transferability to the substrate surface and leveling properties are deteriorated. In particular, the dropping body does not reach the substrate surface, making transfer difficult. If it exceeds 2.0 g, on the contrary, transfer is performed before a sufficient drop is formed, so that stable transfer becomes difficult. The coating amount after drying can be calculated according to the solid content of 20% to 50% of the dispensing liquid 35, and is preferably in the range of 0.1 to 0.8 g (corresponding to 50 to 410 g / m ² ).

[Leveling process]
Thereafter, the porous nozzle 125 is separated upward to be larger than h, so that it diffuses instantaneously through the hemispherical dropping body 35e as shown in FIG. 5 (e) or FIG. 6 (D), and FIG. f) and smoothing by leveling as shown in FIG.

  As the operation start tamming of the discharge control means 115, the state of the separation distance h in FIG. 5A is preferable, but it may be in a descending stage slightly before that. As the operation end tamming of the discharge control means 115, the state of FIG. The holding time at the separation distance h can be appropriately set according to the conditions such as the discharge pressure and the viscosity of the dispensing liquid 35. For example, the holding time is 0.2 to 3.0 seconds.

  By controlling the discharge control means 115 in this way, the volume of the drop body can be maintained within a predetermined range by the surface tension of the drop body mainly derived from the appropriate viscosity of the dispensing liquid 35. In addition to this, by utilizing the wettability of hydrophilic dispensing liquid 35 such as water or alcohol solvent system to hydrophilic substrate sheet 10 such as paper substrate including synthetic paper, the substrate sheet An excellent method capable of exhibiting excellent transferability to 10 and subsequent leveling is the production method of the present invention.

[Continuous formation]
As shown in the lower part of step S2 in FIG. 2, after the dispensing to one frame layer 20 is completed, the X-direction movable means 140 and / or the Y-direction movable means 150 are moved so that the XY coordinates on the base sheet 10 It is of course possible to continuously dispense a plurality of frame layers 20 by changing the position. And the culture layer 30 can be formed in the several location on the base material sheet 10 by combining process S1 and process S2 (process S3).

[Post-process]
Thereafter, the dispense liquid 35 is made into the culture layer 30 through a predetermined drying process by a drying device such as hot air (not shown). The coating thickness after drying is preferably 100 μm to 500 μm. If it is less than 100 μm, the application amount is small and the effect of the present invention cannot be obtained. If it exceeds 500 μm, the proportion of culture layers not used increases, which is uneconomical.

Thereafter, a cover film 40 such as a biaxially stretched polypropylene film is laminated on the base material sheet 10 on which the culture layer 30 is formed, and one end is bonded, and if necessary, individually cut by a cutting device (not shown). By doing so, a microorganism culture sheet can be produced.

  As shown in FIG. 7, the microorganism culture sheet manufactured in this way includes a culture layer 30 on a square base sheet 10. A circular frame layer 20 made of a hydrophobic resin is formed on the outer periphery of the culture layer 30, and the culture layer 30 is formed in a recessed region surrounded by the frame layer 20. Further, there is no gap between the frame layer 20 and the culture layer 30, and both layers are in contact with each other. A rectangular transparent cover sheet 40 is provided so as to cover the frame layer 20 and the culture layer 30.

  According to this microorganism culture sheet, since the circular frame layer 20 is formed on the outer periphery of the culture layer 30, it can be reliably expanded within a predetermined range without leaking the test liquid. Moreover, since the frame layer 20 is formed of a hydrophobic resin, the frame layer 20 is not broken by the test solution even when the test solution is inoculated on the culture layer 30. Furthermore, since the frame layer 20 and the culture layer 30 are in contact with each other, the test solution is not stored in the gap, and the test solution can be spread uniformly on the culture layer 30. By using the dispensing apparatus of the present invention, a culture layer having a uniform thickness that is necessary and sufficient for the growth of microorganisms can be formed, so that colonies are difficult to bleed and a difference in colony formation speed is unlikely to occur. In addition, it is possible to accurately perform a microbial test such as measurement of the number of colonies.

  EXAMPLES Hereinafter, although an Example demonstrates this invention further in detail, this invention does not receive a restriction | limiting at all by these description.

<Manufacture of dispensing liquid>
20 g of polyvinylpyrrolidone (trade name “Polyvinylpyrrolidone K-90”, manufactured by Nippon Shokubai Co., Ltd.) was dissolved in methanol, and 60 g of guar gum powder (trade name “NEOVISCO G”, 400 mesh type, manufactured by Sanki Co., Ltd.) was dispersed therein. . As a nutrient component, tryptone (trade name “BACT TRYPTONE”, Becton, Dickinson and Company) 3.26 g, meat extract (trade name “Lab-Lemco”, Oxoid) 0.82 g, yeast extract ( Trade name “YEAST EXTRACT”, Becton, Dickinson and Company 0.03 g, glucose (trade name “D-(+)-GLUCOSE”, Wako Pure Chemical Industries, Ltd.) 0.16 g, sodium chloride (Wako Pure Chemical) 0.41 g (manufactured by Kogyo Co., Ltd.) and 0.37 g of disodium hydrogen phosphate (manufactured by Wako Pure Chemical Industries, Ltd.) were added, and the amount of methanol was adjusted to prepare a dispensing solution having a solid content ratio of 35%. The viscosity of the dispensing liquid was 150 to 300 cp.

Next, using a dispensing apparatus as shown in FIGS. 1 to 5, a dispensing liquid is discharged under the following conditions using a porous nozzle as shown in FIG. 4, and the transfer and leveling conditions are very good visually (○ ), Good (Δ), and poor (×). The results are shown in Table 1. In addition, Table 2 shows the results evaluated in the same manner as described above except that a dispensing solution having a solid content of 40% was used. Furthermore, the result of having measured the relationship between both by changing application | coating pressure (discharge pressure) and changing application quantity is shown in FIG.
Nozzle hole diameter: as shown in Tables 1 and 2 (unit: mm)
Nozzle hole arrangement density: 10 to 11 / cm ²
Application amount (wet): 510 g / m ²
Application amount (dry): 180 g / m ²
Average coating thickness: 250-300 μm

From Table 1 and Table 2, using the porous nozzle comprised by the nozzle of the hole diameter of 0.9 mm-1.6 mm of this invention, the separation distance with a base-material surface is 0.3 mm-1.0 mm, solid content is 20% to 50%, preferably from can be seen that the leveling 20% to 30% of the dispensed liquid, favorably transferred and the case where the coating amount is ejected so as to 250g ^{/ m 2} ~1050g ^/ m 2 .

  When the separation distance was less than 0.3 mm, a sufficient amount of dripping body could not be formed, so a stable coating amount could not be obtained, and transferability to the substrate surface and leveling properties were reduced. Further, when the nozzle hole diameter is 0.8 mm or less, clogging occurs and the ink cannot be discharged properly, and when it is 1.7 mm, liquid dripping or unevenness after application occurs. If the solid content is 60%, it is difficult to discharge due to thickening, and if the solid content is 10%, a sufficient amount of dripping cannot be formed, so a stable coating amount cannot be obtained. Transferability to the surface and leveling properties decreased.

Further, from FIG. 8, by changing the discharge pressure from 0.054 MPa to 0.057 MPa, in proportion to this pressure, 155 g / m ² (corresponding to the coating amount of 0.31 g · dry in FIG. 8) to 190 g / m. ² (corresponding to a coating amount of 0.37 g · dry in FIG. 8) can be understood to be stably obtained.

DESCRIPTION OF SYMBOLS 10 Base material sheet 20 Frame layer 30 Culture layer 35 Dispensing liquid 35b, 35c, 35d, 35e Dropping body 40 Cover sheet 100 Dispensing apparatus 110 Dispensing liquid supply means 110
114, 115 Hollow tube 116 Discharge pressure control device 117 Valve 118 Discharge pressure control system 120 Dispensing part 130 Z direction movable means 140 X direction movable means 150 Y direction movable means 160 Movable control means K Discharge hole P Divided region

Claims (5)

The separation distance between the discharge surface of the porous nozzle composed of nozzles having a hole diameter of 0.9 mm to 1.6 mm and the substrate surface is set to 0.3 mm to 1.0 mm,
The solid content of 20% to 50% of the dispensing liquid, dispensing the coating amount of the nozzle constituting the porous nozzle ejected so as to ^{250g / m 2 ~1050g / m 2} , to transfer coating to the substrate surface A method for producing a liquid-coated sheet.   The method for producing a dispensing liquid-coated sheet according to claim 1, wherein the viscosity of the dispensing liquid is 100 to 2000 cp. The coating amount of the nozzle constituting the porous nozzle, the manufacturing method of dispensing liquid coating sheet according to claim 1 or 2 is a 50g / m ² ~410g / m ² after drying.   The method for producing a dispensing liquid-coated sheet according to claim 3, wherein the thickness after drying is 100 μm to 500 μm.   The method for producing a dispense liquid-coated sheet according to any one of claims 1 to 4, wherein the dispense liquid-coated sheet is a microorganism culture sheet, and the dispense liquid is a medium liquid for forming a culture layer of the microorganism culture sheet.

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