JP2015042630A - Blood glucose-decreasing agent which works fast when using with exercise - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a blood glucose-decreasing agent which works fast when using with exercise, which is highly safe and can decrease blood glucose level promptly.SOLUTION: A blood glucose-decreasing agent which works fast when using with exercise contains at least any of Protopanaxatriol, Panaxatriol, Protopanaxadiol, and Panaxadiol. The embodiment such that at least any of the Protopanaxatriol, Panaxatriol, Protopanaxadiol, and Panaxadiol are contained in a strong acid-treated material of Araliaceae carrot, or the embodiment such that the strong acid-treated material is obtained by applying a strong acid aqueous solution with a concentration of 0.01-4 mol/L to the Araliaceae carrot to perform hydrolysis treatment under presence of lower alcohol, or the like is preferable.

Description

  The present invention relates to an early blood sugar lowering agent during exercise combined use.

  In recent years, obesity is caused by uneven diet and lack of exercise, and lifestyle-related diseases such as diabetes, hyperlipidemia, and hypertension are increasing. Under such circumstances, the Ministry of Health, Labor and Welfare started “Health Japan 21” in 2000 as a national health promotion in the 21st century. With regard to diabetes, measures such as awareness-raising activities aimed at preventing and improving obesity by improving diet and increasing exercise amount were taken, but no significant effect was obtained. One of the reasons for this is thought to be that the method of improving diet and exercise took time to obtain the effect and it was difficult to continue for a long time. In addition, pharmacotherapy can be mentioned as a method of lowering blood glucose level early, but since there is a risk of hypoglycemia, detailed guidance from a doctor such as a usage volume is essential, and it was difficult to use it easily at the patient's own judgment.

On the other hand, as a method for improving sugar metabolism, for example, a composition containing panaxadiol (PD) or panaxatriol (PT) contained in ginseng ginseng (see, for example, Patent Documents 1 and 2), ginseng ginseng. Or the composition which irradiated the microwave to the extract (for example, refer patent document 3), the foodstuff (for example, refer patent document 4) containing indigestible dextrin, dry powder of ginseng and dry powder of ganoderma A granulated product obtained by shaping the dried powder of Agaricus koji into a granular shape (see, for example, Patent Document 5) has been proposed.
In addition, the applicant of the present application is excellent in a composition containing (A) Panax ginseng, (B) Hibatsu extract, and (C) at least one of vitamin B1, a salt thereof, or a derivative thereof. It has proposed that it has an action to improve sugar metabolism, is highly safe and can be used easily (for example, see Patent Document 6).
However, these proposed compositions have a problem that it is necessary to continuously ingest them for a long period of time in order to obtain the effect of lowering blood glucose level, as in the improvement method by diet and exercise.

If the effect of lowering blood sugar can be confirmed in a short period of time, it can be expected to maintain and improve motivation, and the user can continue to prevent or treat diabetes etc. for a long period of time.
Therefore, at present, there is a strong demand for provision of an early blood glucose lowering agent that is highly safe and has a short period of time until the effect of lowering blood glucose level is obtained and that is effective in the prevention or treatment of diabetes.

JP 2011-026314 A JP 2011-026313 A JP 2002-114696 A JP-A-6-166622 JP 2000-143526 A International Publication No. 2012/141018 Pamphlet

  An object of the present invention is to solve the above-described problems and achieve the following objects. That is, an object of the present invention is to provide an early blood sugar lowering agent at the time of exercise combined use, which has high safety and can lower blood sugar level early.

  The early hypoglycemic agent of the present invention at the time of combined use with exercise as a means for solving the above problems contains at least one of protopanaxatriol, panaxatriol, protopanaxadiol, and panaxadiol.

  According to the present invention, there is provided an early blood sugar lowering agent at the time of exercise combined use, which can solve the conventional problems, achieve the object, has high safety and can lower blood sugar level early. be able to.

FIG. 1 is a graph showing the results of the combined effect of Nana Tanajin Participant Powder and Exercise in Example 1. FIG. 2 is a graph showing the results of the combined effect of panaxatriol and exercise in Example 2. FIG. 3 is a graph showing the results of the combined effect of indigestible dextrin and exercise in Comparative Example 1.

(Early blood sugar lowering agent during exercise)
The early hypoglycemic agent used in combination with exercise of the present invention contains at least one of protopanaxatriol, panaxatriol, protopanaxadiol, and panaxadiol, and further contains other components as necessary. To do.
Moreover, the early blood sugar lowering agent at the time of exercise combined use of this invention contains the acid-processed product of a ginseng, and also contains another component as needed.

  Conventionally, the acid-treated product of at least one of the protopanaxatriol, panaxatriol, protopanaxadiol, and panaxadiol or the Panax ginseng has a blood glucose lowering action and is used as a sugar metabolism improving agent. However, it is not known at all that it has the effect of synergistically working together with exercise to lower blood glucose levels at an early stage. is there.

<When combined with exercise>
The “when combined with exercise” means taking an early hypoglycemic agent while exercising, and the “exercise” is exercise of walking to light running, and 40% VO ₂ max to 70 % VO ₂ max means an exercise intensity of 3 to 6 mets. The frequency is preferably 3 to 5 times a week, and the time is preferably 15 to 60 minutes.
・ Reference 1: How to perceive exercise intensity (Akira Ito: Illustrated: Introduction to Exercise Physiology, p.129, Ishiyaku Shuppan Publishing, 1990), http://www-user.yokohama-cu.ac.jp/~sport /menu/staff/tamaki/edu/training.html
・ Reference 2: Exercise Guidelines 2006 for Health Promotion (Ministry of Health, Labor and Welfare: Exercise Guide, p.7, 2006), http://www.mhlw.go.jp/bunya/kenkou/undou01/pdf/data.pdf

<Protopanaxatriol, Panaxatriol, Protopanaxadiol, and Panaxadiol>
The protopanaxatriol, the panaxatriol, the protopanaxadiol, and the panaxadiol are compounds belonging to danmaran triterpenes.
The protopanaxatriol, the panaxatriol, the protopanaxadiol, and the panaxadiol are those in which a sugar is detached from a plant-derived saponin (glycoside) to form an aglycon body.
There is no restriction | limiting in particular as said plant, Although it can select suitably according to the objective, Araliaceae ginseng is preferable and, among these, the ginseng is more preferable.
The saponin derived from the ginseng ginseng is not particularly limited and may be appropriately selected depending on the intended purpose. For example, ginsenoside-Rg ₁ , notoginsenoside-R ₁ , ginsenoside-Re, ginsenoside-Rb ₁ , ginsenoside- Rd, ginsenoside-Rc, etc. are mentioned.

-How to obtain-
The method for obtaining the protopanaxatriol, the panaxatriol, the protopanaxadiol, and the panaxadiol is not particularly limited and may be appropriately selected depending on the purpose. The method obtained by extracting from the above, the method obtained by enzymatic fermentation of the ginseng ginseng, the method obtained by hydrolyzing the ginseng ginseng, the method obtained by synthesis, and the like. Commercial products can also be used.
There is no restriction | limiting in particular as the method obtained by said extraction, According to the objective, it can select suitably, For example, a supercritical extraction method, HPLC extraction method, etc. are mentioned.
Among these, the method obtained by hydrolysis is preferable, and the method obtained by acid hydrolysis is more preferable. In particular, it is preferable to use the residue of acid hydrolyzate from the ginseng. By hydrolyzing the ginseng ginseng, it is possible to efficiently obtain the protopanaxatriol, the panaxatriol, the protopanaxadiol, and the panaxadiol, and have an excellent early blood glucose lowering in combination with excellent exercise This is advantageous in that it can exert its action. The residue of the ginseng acid hydrolyzate has advantages such as better taste than the protopanaxatriol, the panaxatriol, the protopanaxadiol, and the panaxadiol itself. In some cases, it is preferable to use the residue of the acid hydrolyzate.

  In addition, the araliaceae ginseng may be used as it is collected from nature. For example, it may be used after pretreatment appropriately combining washing, drying, cutting, crushing, pulverization and the like. . Among these, it is possible to efficiently obtain the protopanaxatriol, the panaxatriol, the protopanaxadiol, and the panaxadiol by using a powdered carrot as the ginseng. This is preferable.

--Hydrolysis treatment--
There is no restriction | limiting in particular as the processing method of the said hydrolysis, According to the objective, it can select suitably, For example, the method etc. which make strong acid aqueous solution of a desired density | concentration act on the said Araliaceae ginseng are mentioned.
The strong acid aqueous solution is not particularly limited as long as it is an aqueous solution containing a strong acid, and can be appropriately selected according to the purpose, but an aqueous solution containing an inorganic acid such as hydrochloric acid, phosphoric acid, sulfuric acid, nitric acid is preferable, and hydrochloric acid An aqueous solution containing is more preferred.

There is no restriction | limiting in particular as a density | concentration of the acid in the said strong acid aqueous solution, Although it can select suitably according to the objective, 0.01 mol / L-4 mol / L are preferable, and 0.5 mol / L-3 mol / L are more preferable.
When the acid concentration is less than 0.01 mol / L, hydrolysis is insufficient, and the protopanaxatriol, the panaxatriol, the protopanaxadiol, and the panaxadiol can be obtained efficiently. When the amount exceeds 4 mol / L, problems such as excessive hydrolysis and disadvantages in cost occur.
On the other hand, when the acid concentration is within the preferable range, the protopanaxatriol, the panaxatriol, the protopanaxadiol, and the panaxadiol can be efficiently obtained by sufficient hydrolysis. This is advantageous.

There is no restriction | limiting in particular as the usage-amount of the said strong acid aqueous solution, Although it can select suitably according to the objective, It is preferable to use a 2 times volume-20 times capacity | capacitance with respect to the said Araliaceae ginseng.
If the amount of the strong acid aqueous solution used is less than twice the capacity of the ginseng ginseng, the ginseng ginseng is not sufficiently soaked and the hydrolysis treatment becomes insufficient. There is a disadvantage in terms of cost.

--- Use of lower alcohols ---
The hydrolysis treatment is preferably performed in the presence of a lower alcohol.
By using the lower alcohol, it is possible to improve the affinity between the ginseng ginseng and the strong acid aqueous solution, and to proceed the hydrolysis efficiently.
There is no restriction | limiting in particular as said lower alcohol, Although it can select suitably according to the objective, Methanol, ethanol, and propanol are preferable and ethanol is more preferable from a safety point.

There is no restriction | limiting in particular as the usage-amount of the said lower alcohol, Although it can select suitably according to the objective, 1 volume%-80 volume% are preferable with respect to the total amount of a hydrolysis liquid, 10 volume%-50 volume % Is more preferable, and 20% by volume to 40% by volume is still more preferable.
When the amount of the lower alcohol used is less than 1% by volume relative to the total amount of the hydrolyzed solution, the protopanaxatriol, the panaxatriol, the protopanaxadiol, and the panaxadiol can be efficiently obtained. If it exceeds 80% by volume, the protopanaxatriol, the panaxatriol, the protopanaxadiol, and the panaxadiol cannot be efficiently obtained, and the cost is disadvantageous. There are things to become.
On the other hand, when the amount of the lower alcohol used is within the preferable range, it is advantageous in that the protopanaxatriol, the panaxatriol, the protopanaxadiol, and the panaxadiol can be obtained efficiently. It is.
The “total amount of hydrolyzed liquid” refers to the total amount of the reaction liquid including the strong acid aqueous solution and the lower alcohol.

--- Total amount of hydrolyzed liquid ---
The total amount of the reaction solution (total amount of hydrolysis solution) including the strong acid aqueous solution and the lower alcohol is not particularly limited and may be appropriately selected depending on the intended purpose. A double capacity to 20-fold capacity is preferred.
When the total reaction liquid amount is less than twice the volume of the carrot ginseng, the carrot carrot is not sufficiently immersed, resulting in insufficient hydrolysis, and the like. There is a disadvantage in terms of cost.

--- Hydrolysis treatment temperature ---
There is no restriction | limiting in particular as processing temperature in the said hydrolysis process, Although it can select suitably according to the objective, 60 to 100 degreeC is preferable and 70 to 90 degreeC is more preferable.
When the treatment temperature is less than 60 ° C., hydrolysis is insufficient and the protopanaxatriol, the panaxatriol, the protopanaxadiol, and the panaxadiol cannot be obtained efficiently. If the temperature exceeds 100 ° C., special production equipment is required, which may be disadvantageous in terms of cost.
On the other hand, when the treatment temperature is within the preferred range, it is advantageous in that the protopanaxatriol, the panaxatriol, the protopanaxadiol, and the panaxadiol can be obtained efficiently.

--- Hydrolysis treatment time ---
There is no restriction | limiting in particular as processing time in the said hydrolysis process, Although it can select suitably according to the objective, 30 minutes-24 hours are preferable, and 2 hours-8 hours are more preferable.
When the treatment time is less than 30 minutes, hydrolysis is insufficient and the protopanaxatriol, the panaxatriol, the protopanaxadiol, and the panaxadiol cannot be obtained efficiently. Yes, if it exceeds 24 hours, the reaction may proceed excessively, and the cost may be disadvantageous.
On the other hand, when the treatment time is within the preferable range, it is advantageous in that the protopanaxatriol, the panaxatriol, the protopanaxadiol, and the panaxadiol can be obtained efficiently.

--- Neutralization treatment--
After the hydrolysis treatment, it is preferable to neutralize the obtained solution after the hydrolysis treatment. There is no restriction | limiting in particular as the method of neutralizing, It can carry out by a well-known method, for example, the method of adding strong base aqueous solution, such as sodium hydroxide and potassium hydroxide, to the liquid after the said hydrolysis process suitably, etc. Is mentioned. In addition, there is no restriction | limiting in particular as pH after the said neutralization, Although it can select suitably according to the objective, pH 5-pH8 are preferable.

-Filtration treatment-
The liquid after the hydrolysis treatment after the neutralization step is preferably filtered and separated into a filtrate and a residue, and the residue is more preferably used. There is no restriction | limiting in particular as the method of filtering, It can carry out by a well-known method. After filtration, washing with water may be repeated until there is no more salt.

--- Hydrofiltration ---
When the lower alcohol is not used in the hydrolysis treatment step, the filtration treatment can be performed as it is after neutralization. However, when the lower alcohol is used, the protopanaxatriol, the panaki are filtered before filtration. In order to promote the residue of satriol, the protopanaxadiol, and the panaxadiol in the residue, it is preferable to add water to lower the lower alcohol concentration in the solution after the hydrolysis treatment.
The amount of water to be added in this case is not particularly limited and can be appropriately selected according to the purpose. The larger the amount, the more preferable, but the lower alcohol concentration in the liquid after hydrolysis treatment is 0.05% by volume or more. It is more preferable to add so that it may become 50 volume% or less, It is still more preferable to add so that it may become 30 volume% or less, It is especially preferable to add so that it may become 10 volume% or less.
When the lower alcohol concentration in the liquid after the hydrolysis treatment exceeds 50% by volume and is subjected to filtration, the protopanaxatriol, the panaxatriol, the protopanaxadiol, and the panaxadiol are lower in level. It is disadvantageous in that it is dissolved in alcohol and discharged as a filtrate, and the content in the residue is reduced.
On the other hand, if the lower alcohol concentration in the liquid after the hydrolysis treatment is within the more preferable range, the protopanaxatriol, the panaxatriol, the protopanaxadiol, and the panaxadiol residue in the residue This is advantageous in that the content can be further increased.

--- Drying process--
The residue after the filtration step may be used as it is as the protopanaxatriol, the panaxatriol, the protopanaxadiol, and the panaxadiol, or may be used after drying.
There is no restriction | limiting in particular as the method of drying, It can carry out by a well-known method, For example, a freeze-drying method, ventilation drying method, heat drying method, reduced pressure drying method etc. are mentioned.

-Content-
The content of the protopanaxatriol, the panaxatriol, the protopanaxadiol, and the panaxadiol contained in the early blood glucose lowering agent at the time of the exercise combination is not particularly limited, and Within a range that does not impair the effect, it can be appropriately selected according to the purpose. In addition, the early blood glucose lowering agent at the time of exercise combination may be at least one of the protopanaxatriol, the panaxatriol, the protopanaxadiol, and the panaxadiol itself.

<Other ingredients>
The other components are not particularly limited and may be appropriately selected depending on the purpose. For example, from among pharmacologically acceptable carriers, the dosage form of an early blood glucose lowering agent at the time of combined use with exercise may be used. It can be appropriately selected depending on the case, and examples thereof include ethanol, water, and starch. Moreover, when using the early blood glucose lowering agent at the time of the said exercise combination for the food / beverage products mentioned later, as said other component, various auxiliary raw materials or additives are mentioned, for example. There is no restriction | limiting in particular also as content of the said other component, According to the objective, it can select suitably.

<Early blood glucose lowering effect when combined with exercise>
The early blood glucose lowering action of the early blood glucose lowering agent during exercise combined with exercise refers to an action of lowering the blood sugar level early when exercise is used together. The term “early blood sugar lowering” means that the blood sugar level significantly decreases in a short period of time as compared with the period necessary for significantly lowering the blood sugar level only by ingesting or exercising the early blood sugar lowering agent. Examples of the blood glucose level include a fasting blood glucose level, and an occasional blood glucose level in which the blood glucose level is measured without determining the time after meal, and any of them may be used, but it is preferable to use the blood glucose level as an index at any time.

<Dosage form>
There is no restriction | limiting in particular as a dosage form of the said early blood glucose lowering agent, According to the objective, it can select suitably, For example, an oral solid agent, an oral semi-solid agent, an oral liquid agent etc. are mentioned. Among these, oral solid preparations are preferable.

-Oral solid agent-
The oral solid preparation is not particularly limited and may be appropriately selected depending on the intended purpose. For example, tablets, chewable tablets, effervescent tablets, orally disintegrating tablets, troches, drops, hard capsules, soft capsules Agents, granules, powders, pills, dry syrups, soaking agents and the like. Among these, capsules are preferable.
Examples of the capsule include No. 1 S. Rock, cap: XKZ HP BROWN5, body: XKZ HP BROWN5 manufactured by Clio Caps Co., Ltd., and the like.

-Oral semi-solid formulation-
There is no restriction | limiting in particular as said oral semi-solid preparation, According to the objective, it can select suitably, For example, a licking agent, a chewing gum agent, a whipping agent, a jelly agent etc. are mentioned.

-Oral solution-
There is no restriction | limiting in particular as said oral liquid agent, According to the objective, it can select suitably, For example, a syrup agent, a drink agent, a suspension agent, an alcoholic agent etc. are mentioned.

  There is no restriction | limiting in particular as a manufacturing method of the said sugar metabolism improving agent, According to dosage form etc., it can select suitably from well-known methods.

<Ingestion>
There is no restriction | limiting in particular as an ingestion method of the early blood glucose lowering agent at the time of the said exercise combination, ingestion amount, ingestion frequency, ingestion time, and ingestion object, According to the objective, it can select suitably.
There is no restriction | limiting in particular as said ingestion method, Although it can select suitably according to the objective, The method of ingesting orally is preferable at the point which is easy to continue since it can ingest easily.
The intake amount is not particularly limited and may be appropriately selected in consideration of various factors such as the age, weight, constitution, symptom, and presence / absence of administration of a drug containing another component as an active ingredient. However, the daily intake is preferably 45 mg to 500 mg, more preferably 90 mg to 270 mg. Within the preferred range, it is advantageous in that it can exhibit an excellent early blood glucose lowering effect during exercise combined use.
There is no restriction | limiting in particular as said frequency | count of ingestion, Although it can select suitably according to the objective, Once a day is preferable at the point with the convenience.
The intake time is not particularly limited and can be appropriately selected depending on the purpose. The early blood sugar lowering agent may be taken before, after or during exercise. Absent.
In addition, in order to reduce the annoyance related to taking for the user, the intake time should not be limited at the same time as meals or after meals. It is preferable that the lowering effect is exhibited. However, if the form to be taken is a normal food, and the dosage form can be taken without any trouble in the meal, the early blood glucose lowering effect at the time of combined use with exercise does not differ depending on the time of intake. It is not related to non-simultaneous intake.
The animal species to be ingested are those that are suitably applied to humans, but as long as their effects are exerted, animals other than humans (eg, mice, rats, hamsters, birds, dogs, Cats, sheep, goats, cows, pigs, monkeys, etc.).

<Use>
The early blood glucose lowering agent at the time of the exercise combined use may be used alone or in combination of two or more, and may be used in combination with a medicine containing other components as active ingredients. Moreover, the early blood sugar lowering agent at the time of the exercise combined use may be used in a state of being blended in a medicine containing another component as an active ingredient.

<Application>
Since the early blood sugar lowering agent at the time of exercise combined use has an excellent early blood glucose lowering effect at the time of exercise combined use, it can be suitably used for foods and drinks described later.

<Food &Drink>
The food and drink used in the present invention contains the early blood sugar lowering agent at the time of exercise combination, and further contains other components as necessary.
Here, the food and drink are those which are less likely to harm human health and are taken by oral or gastrointestinal administration in normal social life. It is not limited to the category of goods, etc., and means, for example, a wide range of foods that are taken orally, such as general foods, health foods, health functional foods, quasi drugs, and pharmaceuticals.

There is no restriction | limiting in particular as a compounding quantity of the early blood glucose lowering agent at the time of the said exercise combined use in the said food / beverage products, and it mix | blends suitably according to the kind of food / beverage products used as long as the effect of this invention is not impaired. Can do.
The food and drink may contain only the early blood sugar lowering agent during the exercise combination, and the food and drink may be the early blood sugar lowering agent itself during the exercise combination.

-Types of food and drink-
There is no restriction | limiting in particular as said kind of food / beverage products, According to the objective, it can select suitably, For example, drinks, such as a soft drink, a carbonated drink, a nutritive drink, a fruit drink, a lactic acid drink; Ice cream, ice sherbet, Frozen confectionery such as shaved ice; noodles such as buckwheat, udon, harusame, gyoza peel, sushi mai, Chinese noodles, instant noodles; rice cake, candy, gum, chocolate, tablet confectionery, snack confectionery, biscuits, jelly, jam, cream, baked Confectionery such as confectionery and bread; crab, salmon, clams, tuna, sardines, shrimp, skipjack, mackerel, whale, oyster, saury, squid, red scallop, scallop, abalone, sea urchin, salmon roe, tocobushi, etc .; Processed fishery and livestock products such as sausages; dairy products such as processed milk and fermented milk; salad oil, tempura oil, margarine, ma Oils and processed oils such as nauses, shortenings, whipped creams, dressings; seasonings such as sauces and sauces; curry, stew, oyakodon, rice cakes, miscellaneous cooking, Chinese rice cakes, bowls, tempura, eel rice, hayashi rice, oden, Retort pouch foods such as Mabodorf, beef bowl, meat sauce, egg soup, omelet rice, dumplings, shumai, hamburger, meatballs; various forms of health foods, nutritional supplements, pharmaceuticals, quasi drugs, and the like.

<Other ingredients>
There is no restriction | limiting in particular as said other component, According to the objective, it can select suitably, For example, the auxiliary | assistant raw material or additive etc. which are normally used in manufacturing food-drinks are mentioned.
The auxiliary raw material or additive is not particularly limited and may be appropriately selected depending on the intended purpose. For example, glucose, fructose, sucrose, maltose, sorbitol, stevioside, rubusoside, corn syrup, lactose, citric acid , Tartaric acid, malic acid, succinic acid, lactic acid, L-ascorbic acid, dl-α-tocopherol, sodium erythorbate, glycerin, propylene glycol, glycerin fatty acid ester, polyglycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, Arabia Examples include gum, carrageenan, casein, gelatin, pectin, agar, vitamin Bs, nicotinic acid amide, calcium pantothenate, amino acids, calcium salts, pigments, fragrances, preservatives and the like.
There is no restriction | limiting in particular as content of the said other component, According to the objective, it can select suitably.

  EXAMPLES Hereinafter, although an Example is given and this invention is demonstrated in detail, this invention is not limited to these Examples at all.

<Exercise intensity>
Exercise intensity is the exercise of about walking-light _running, corresponding to _{40% VO 2 max~70% VO 2} max, 3 Metz 6 Metz. The frequency is preferably 3 to 5 times a week, and the time is preferably 15 to 60 minutes.
The exercise imposed on the mouse in the following examples was “15 m / min, 45 min, tilt angle 0 °, 5 days / week, and treadmill running exercise for 38 days” corresponding to the exercise intensity in humans.
・ Reference 1: How to perceive exercise intensity (Akira Ito: Illustrated: Introduction to Exercise Physiology, p.129, Ishiyaku Shuppan Publishing, 1990), http://www-user.yokohama-cu.ac.jp/~sport /menu/staff/tamaki/edu/training.html
・ Reference 2: Exercise Guidelines 2006 for Health Promotion (Ministry of Health, Labor and Welfare: Exercise Guide, p.7, 2006), http://www.mhlw.go.jp/bunya/kenkou/undou01/pdf/data.pdf

(Production Example 1)
<Nanata Participant Engineering Powder (Acid Treatment)>
1 kg of ginseng powder (manufactured by Matsuura Pharmaceutical Co., Ltd.) is suspended in 10 L of a 25 wt% aqueous ethanol solution containing 5.9 wt% hydrochloric acid (2 mol / L hydrochloric acid) and reacted at 70 ° C. for 6 hours with slow stirring. I let you. Subsequently, after cooling this reaction liquid on ice, 5 mol / L sodium hydroxide aqueous solution was added and it adjusted to pH 7.0. Next, the pH-adjusted solution was diluted 10-fold with distilled water, suction filtered, and separated into a filtrate and a residue. The obtained residue was freeze-dried to obtain 180 g of Tanashi Ninjin Participant Powder.
The contents of panaxatriol (PT) and panaxadiol (PD) in the obtained Nanata Participant's powder were analyzed and measured by the following method. The content of PT was 6.1% by mass. The PD content was 4.6% by mass.

<Analysis of PT and PD>
About 0.1 g of Tanashi Ninjin Participation Powder was precisely weighed, added with about 8 mL of ethanol (purity 99.5% by volume), and suspended for 15 minutes using an ultrasonic bath. After centrifugation at about 700 × g for 10 minutes, ethanol (purity 99.5% by volume) was added to the supernatant to make exactly 10 mL. This liquid was measured by gas chromatography under the following conditions. The retention time of PT under the following conditions was about 29 minutes, and the retention time of PD was about 18 minutes.
[Analysis conditions]
Gas chromatograph: GC353B (GL Science Co., Ltd.)
Detector: Hydrogen flame ionization detector (FID)
Injection method: Split injection method (split ratio 1:50)
Column: DB-17MS (length 30 m, inner diameter 0.25 mm, film thickness 0.25 μm, manufactured by Agilent Technologies)
Column temperature: Initial temperature: 310 ° C
Initial temperature holding time: 20 minutes
Temperature increase rate: 10 ° C / min
Achieving temperature: 320 ° C
Achieving temperature holding time: 14 minutes Carrier gas: Helium Flow rate: 1.5 mL / min Inlet temperature: 320 ° C
Detector temperature: 320 ° C
Injection volume: 1 μL

  Panaxatriol standard product (manufactured by LKT Laboratories) and panaxadiol standard product (manufactured by LKT Laboratories) were prepared to 1 mg / mL, 0.5 mg / mL, and 0.1 mg / mL, respectively, and a calibration curve was prepared. A standard solution was prepared. The standard solution for calibration curve was measured by gas chromatography under the same conditions as described above using 1 μL each. Each peak area was measured, and a calibration curve was prepared from the peak area and concentration of each standard curve standard solution. Using this calibration curve, the contents of PT and PD in the Nanajin Participant's powder were measured.

<Panaxatriol, Panaxadiol>
An ethanol solution containing 3% by mass of the Nanajin Participant's powder was prepared. Next, after removing insolubles using filter paper, the solution is further concentrated 8 times using a rotary evaporator, and the concentrated solution is added to a glass column packed with silica gel (silica gel 60N, manufactured by Kanto Chemical Co., Inc.), Column fractionation was performed using chloroform: ethanol = 10: 1 (V / V) as an eluent. On normal phase TLC using chloroform: ethanol = 10: 1 (V / V) as a developing solvent, the fraction corresponding to an Rf value of 0.4 was concentrated to obtain highly pure panaxatriol (PT). It was. Further, the fraction corresponding to an Rf value of 0.6 was concentrated to obtain highly pure panaxadiol (PD).

<Measurement of blood glucose level>
The blood glucose level was measured with a simple blood glucose meter (trade name: One Touch Ultra, manufactured by Johnson & Johnson).

(Example 1: About the combined effect of Nanatsu Participant's powder and exercise)
Hyperglycemia model mice (KKAy mice (CLEA Japan, Inc., 5-week-old male, 6 mice / group) were preliminarily raised for 5 days and acclimatized. (1) Control group (no exercise load), (2) Nana Nana Participant Powder Group (no exercise load, Nana Ninjin Participant Powder 2% mixed diet), (3) The group was divided into an exercise group (with exercise load), and (4) exercise + Tanashi Participant Participant Powder Group (with exercise load, Tabata Seven Participant Participant Powder 2% mixed administration). The feed (CE-2, Nippon Claire) was given 5.5g per day so that it could be taken before and after exercise. In addition, water was freely consumed.
The exercise was performed under conditions of 15 m / min, 45 min, an inclination angle of 0 °, and 5 days / week, and the treadmill running exercise was performed for 38 days. After the start of exercise (initial value), blood glucose levels were measured at any time on the 10th, 17th, 31st, and 38th days. The results are shown in Table 1, Table 2, and FIG. For statistical calculation, Student's t-test was used, and a significance level of less than 5% was regarded as a significant difference by two-sided test.

<Changes in blood glucose level as needed>
Mean value ± standard error, n = 6, student's t-test (vs control group)
**: p <0.01, *: p <0.05

<Effects on blood glucose level as needed>
**: p <0.01, *: p <0.05

  From the results of Tables 1 and 2 and FIG. 1, the blood glucose level of the control group gradually increased from the start of exercise. In the exercise group, the blood glucose level was significantly lower than that in the control group after 31 days from the start of exercise. On the other hand, compared with the control group, the blood glucose level was significantly lower after the 10th day from the start of the exercise in the exercise + fielder participation group powder group. During the whole period, the exercise group and the seven participants participated and the powder group showed the lowest blood glucose level. It was recognized that the increase in blood glucose level was suppressed earlier than the exercise alone, by combining the intake of powder and customary exercise.

(Example 2: combined effect of panaxatriol and exercise)
Hyperglycemia model mice (KKAy mice (CLEA Japan, Inc., 5-week-old male, 8 mice / group) were preliminarily raised for 5 days and acclimatized. (1) Control group (no exercise load), (2) PT group (no exercise load, PT 0.24% mixed diet), (3) Exercise group (with exercise load) ), (4) Exercise + PT group (with exercise load, PT 0.24% mixed administration) was divided into groups. The feed (CE-2, Nippon Claire) was given 5.5g per day so that it could be taken before and after exercise. In addition, water was freely consumed.
The treadmill running exercise was performed for 38 days under conditions of 15 m / min, 45 min, an inclination angle of 0 °, and 5 days / week. After the start of exercise (initial value), blood glucose levels were measured at any time on the 10th, 17th, 31st, and 38th days. The results are shown in Tables 3 and 4 and FIG. For statistical calculation, Student's t-test was used, and a significance level of less than 5% was regarded as a significant difference by two-sided test.

<Changes in blood glucose level as needed>
Mean value ± standard error, n = 8, student's t-test (vs control group)
***: p <0.001, **: p <0.01, *: p <0.05

<Effects on blood glucose level as needed>
***: p <0.001, **: p <0.01, *: p <0.05

  From the results of Table 3, Table 4, and FIG. 2, the blood glucose level gradually increased in the control group from the start of exercise. In the exercise group, the blood glucose level was significantly lower than that in the control group after 31 days from the start of exercise. On the other hand, in the exercise + PT group, the blood glucose level was significantly lower than that in the control group after the 10th day from the start of exercise. In all periods, exercise + PT group showed the lowest blood glucose level. It was confirmed that the combined use of PT intake and habitual exercise suppresses an increase in blood glucose level earlier than exercise alone. Similar effects were observed for protopanaxatriol, panaxadiol, and protopanaxadiol.

(Comparative example 1: About the combined effect of indigestible dextrin and exercise)
Hyperglycemia model mice (KKAy mice (CLEA Japan, Inc., 5-week-old male, 8 mice / group) were preliminarily raised for 5 days and acclimatized. (1) Control group (no exercise load), (2) Indigestible dextrin group (no exercise load, indigestible dextrin (Matsutani Chemical Industry Co., Ltd.) 2% mixed diet so that body weight and blood glucose level are equal ), (3) Exercise group (with exercise load), (4) Exercise + indigestible dextrin group (with exercise load, indigestible dextrin (Matsuya Chemical Industry Co., Ltd. 2% dietary administration)) . The feed (CE-2, Nippon Claire) was given 5.5g per day so that it could be taken before and after exercise. In addition, water was freely consumed.
The exercise was performed under conditions of 15 m / min, 45 min, an inclination angle of 0 °, and 5 days / week, and the treadmill running exercise was performed for 38 days. After the start of exercise (initial value), blood glucose levels were measured at any time on the 10th, 17th, 31st, and 38th days. The results are shown in Table 5, Table 6, and FIG. For statistical calculation, Student's t-test was used, and a significance level of less than 5% was regarded as a significant difference by two-sided test.

<Changes in blood glucose level as needed>
Mean value ± standard error, n = 8, student's t-test (vs control group)
**: p <0.01, *: p <0.05

<Effects on blood glucose level as needed>
**: p <0.01, *: p <0.05

  From the results of Tables 5 and 6, and FIG. 3, the blood glucose level gradually increased in the control group from the start of exercise. In the indigestible dextrin group, the blood sugar level was significantly lower than that in the control group on the 38th day after the start of exercise. In the exercise group, the blood glucose level was significantly lower than that in the control group after 31 days from the start of exercise. Furthermore, in the exercise + indigestible dextrin group, the blood glucose level was significantly lower than that in the control group after 31 days from the start of exercise. Even when indigestible dextrin intake and habitual exercise were used in combination, it was not recognized that the blood glucose level decreased earlier compared to exercise alone.

As an aspect of this invention, the following are mentioned, for example.
<1> An early hypoglycemic agent for combined use with exercise, comprising at least one of protopanaxatriol, panaxatriol, protopanaxadiol, and panaxadiol.
<2> Early blood glucose during exercise combined use according to <1>, wherein at least one of protopanaxatriol, panaxatriol, protopanaxadiol, and panaxadiol is contained in the strongly acid-treated product of ginseng ginseng It is a reducing agent.
<3> The product according to <2>, wherein the strong acid-treated product is obtained by applying a strong acid aqueous solution having a concentration of 0.01 mol / L to 4 mol / L to Carrot ginseng and performing a hydrolysis treatment in the presence of a lower alcohol. It is an early hypoglycemic agent when combined with exercise.
<4> An early hypoglycemic agent for combined use with exercise, characterized by containing an acid-treated product of a ginseng.
<5> The early blood glucose lowering agent for exercise combined use according to <4>, wherein the acid-treated product of the ginseng contains at least one of panaxatriol and panaxadiol.
<6> A food or drink comprising the early blood sugar lowering agent during exercise combination according to any one of <1> to <5>.

  Since the early blood sugar lowering agent at the time of exercise combination of the present invention has an excellent early blood glucose lowering action at the time of exercise combination, the user can confirm the effect at an early stage, so that it is easy to continue for a long period of time, such as diabetes It is effective for prevention or treatment. Furthermore, since the early blood sugar lowering agent at the time of the exercise combination is a natural product and high safety, it can be suitably used for food and drink.

Claims (4)

  An early hypoglycemic agent for combined use with exercise, comprising at least one of protopanaxatriol, panaxatriol, protopanaxadiol, and panaxadiol.   The early blood glucose lowering agent at the time of combined use of exercise according to claim 1, wherein at least one of protopanaxatriol, panaxatriol, protopanaxadiol, and panaxadiol is contained in a strongly acid-treated product of the ginseng ginseng.   The strong acid-treated product is obtained by allowing a strong acid aqueous solution having a concentration of 0.01 mol / L to 4 mol / L to act on a carrot ginseng and subjecting it to hydrolysis treatment in the presence of a lower alcohol. Early blood sugar lowering agent.   An early hypoglycemic agent for use in combination with exercise, characterized by containing an acid-treated product of Tanana ginseng.