JP2012031073A - Antistress agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide safe antistress agent which can effectively prevent or alleviate a variety of symptoms resulting from stress, and is free from a side effect and addictiveness, and to provide food and drink, medicines and an aromatic composition containing the antistress agent.SOLUTION: There is provided the antistress agent which contains, as effective components, an extractive of a mint-family angustifolia plant and a polyphenol-containing fraction obtained by fractionalizing the extractive. There are also provided the food and drive, the medicines and the aromatic composition which contain these components.

Description

  The present invention relates to an anti-stress agent effective for prevention or alleviation of mental and physical diseases caused by various stresses, and food and drink, pharmaceuticals, and fragrance compositions containing the same.

  Stress refers to the overall image of strain generated in the body and the resulting defense response to various stimuli (stressors) applied from outside the body. Here, the stressor originally includes physical things (temperature, sound, electromagnetic waves, etc.), chemical things (drugs, heavy metals, etc.), biological things (starvation, infection, lack of oxygen, etc.). However, the stressors received by people living in modern society account for a large percentage of psychological and social issues such as relationships, work, study, family issues, spouse death, and divorce.

  When a stressor is perceived as threatening, harmful, or challenging, it causes emotional changes such as anxiety, tension, anger, excitement, and fear, followed by nervous, endocrine, or immune Through the system, various body reactions such as increased blood pressure, increased heart rate, increased blood sugar, dilated pupil, vasoconstriction in skin and viscera, vasodilation in muscle and brain, gastrointestinal dysfunction, and sweating occur. These reactions are reactions that are necessary for the living body to maintain homeostasis, and moderate stress rather increases individual productivity.

  However, if the stress exceeds a certain limit, homeostasis breaks down, causing damage to the mind and body. Excessive stress causes various physical illnesses such as anxiety disorders and depression, as well as gastric / duodenal ulcers, hypertension, irritable bowel syndrome, atopic dermatitis, lifestyle-related diseases.

  Conventionally, drugs such as tranquilizers, anti-anxiety agents and hypnotics have been used to reduce mental and physical symptoms due to stress. However, problems of addictiveness and side effects have been pointed out with these drugs, and frequent use for the purpose of alleviating daily stress is not preferable from the viewpoint of safety.

  In addition, in order to overcome the above-mentioned problems of drugs, antistress agents using food materials or food additives have been proposed. For example, a stress-improving food combining glutathione and other antioxidants (Patent Document 1), a stress inhibitor containing gluten degradation products as an active ingredient (Patent Document 2), and a solvent extract of a plant belonging to the family Oysteraceae Anti-stress composition (Patent Document 3), anti-stress agent containing pine bark extract (Patent Document 4), anti-stress agent containing horseradish leaf extract (Patent Document 5) and the like are known. Yes. However, these anti-stress agents are not necessarily sufficient in terms of effects.

  Labiatae Lavandula genus plants are perennial shrubs native to the Mediterranean coast, and essential oils prepared by methods such as steam distillation from the above-ground parts (flowers, leaves, stems, etc.) of the plants, Traditionally, it is said to have antibacterial action, antifungal action, sedative action, sedative action and antidepressant action (Non-Patent Document 1).

  Today, for the purpose of preventing or alleviating various symptoms caused by stress, aroma therapy (aromatherapy) using essential oils of Labiatae plants of Lamiaceae is commonly used. However, the aroma therapy has a great influence on the effect because the preference for fragrance has a great effect on the effect, and it is difficult to keep the inhalation amount of essential oil vapor constant, so the effect is not stable. There's a problem.

  In addition, for the same purpose, compositions that can be taken orally have been proposed containing essential oils of Labiatae labranula plants or their components (for example, linalool) as active ingredients (Patent Documents 6, 7, and 8). However, when the above essential oil or its components are taken orally, if the intake is in the range usually contained in foods and beverages, it is probably safe (Non-Patent Document 2), but it is not always sufficient in terms of effect. I could not say. On the other hand, high-dose oral intake of the essential oil is considered an anesthetic poison (Non-Patent Document 3), and it cannot be said that sufficient safety is ensured.

  As described above, any conventional anti-stress agent is not an ideal anti-stress agent in terms of safety or effectiveness, it is truly effective, and safe anti-stress that can be taken with peace of mind. The development of stress agents has been desired.

JP-A-8-275752 Japanese Patent Laid-Open No. 11-49697 Special Table 2000-136143 JP 2003-95964 A JP 2008-230975 A Special table 2008-515833 JP 2004-194590 A JP 2004-123655 A

Phytotherapy Research, 2002, 16: 301-308 “Health Food Database”, Daiichi Publishing, 2007, 313-314 “Encyclopedia of Natural Foods, Pharmaceuticals, and Cosmetics”, Asakura Shoten, 1999, pages 437-439

  The problem to be solved by the present invention is to effectively prevent or reduce various symptoms caused by stress, and to provide a safe anti-stress agent having no side effects and habits, and a food, beverage, medicine, and fragrance composition containing the same. Is to provide.

  As a result of intensive research to solve the above-mentioned problems, the present inventors have found that extracts of Labiatae belonging to the genus Labiatae and polyphenol-containing fractions obtained by fractionating the plant extracts are various factors resulting from stress. It has been found that there is an effect of preventing or reducing symptoms, particularly stress gastric ulcer and duodenal ulcer and stress gastritis, and the present invention has been completed.

That is, the present invention
(1) an anti-stress agent comprising a Labiatae lavandula plant as an active ingredient,
(2) an anti-stress agent comprising an extract of a Labiatae plant of Lamiaceae as an active ingredient,
(3) an anti-stress agent containing, as an active ingredient, a polyphenol-containing fraction obtained by fractionating an extract of a Labiatae plant belonging to the genus Labiatae
(4) Labiatae Lavandula, Lavandula angustifolia, Spike lavender, Lavandula latina, Lavandula x intermeda, St estra lavender (Lavandula pedunculata), Willidis lavender (Lavandula viridis), Dentara lavender (Lavandula dentata), Multifida lavender (Lavandula multifida) and Pinata lavender (Lavandula pinna venta) -At least one selected from the group consisting of a cross of Lavandula angustifolia and Woolley lavender and a cross of Lavandula lavender and Lavandula viridis The antistress agent according to any one of to (3).

Furthermore, the present invention provides
(5) The antistress agent according to any one of (1) to (4) above, for preventing or improving stress gastric ulcer and duodenal ulcer and stress gastritis,
(6) An anti-stress food or drink comprising the anti-stress agent according to any one of (1) to (5),
(7) The antistress agent comprising the antistress agent according to any one of (1) to (5), and (8) any one of (1) to (5) It contains the antistress agent for antistress characterized by including the antistress agent of description to term.

  The antistress agent of the present invention is safer than habituality and side effects as compared with tranquilizers, anti-anxiety agents, and sleeping pills. Moreover, it is safe and has an excellent anti-stress action as compared with a composition for oral intake using a conventional Labiatae plant of the Labiatae family.

UV absorption spectrum of antistress agent of Production Example 1 UV absorption spectrum of antistress agent of Production Example 2 The graph which showed the influence on the stress gastric ulcer by the oral intake of the antistress agent of the manufacture example 2 The graph which showed the influence on the stress gastric ulcer by the oral intake of R-(-)-linalool (comparative example 1) and 1,8- cineole (comparative example 2).

(1) Anti-stress agent As a raw material of the Labiatae Lavandula genus which is a raw material of the anti-stress agent of the present invention, True lavender (Lavandula angustifolia), Spike lavender (Lavandula latifolia), Wool lavender (Lavandula lanta), Lavandula (Landula) , Stoskas lavender (Lavandula stoechas), Pedunclata lavender (Lavandula pedunculata), Willidis lavender (Lavandula viridais), Dentara lavender (Lavandula dentava) dula pinnat) and hybrids of Lavandula angustifolia and Woolley lavender (Lavandula lanata), and crosses of Stavus lavender (Lavandula stochas) and Wiridis lavender, especially Vendula lavender, Spike Lavandin is preferred.

  As the anti-stress agent of the present invention, the above-ground parts (flowers, leaves, stems, etc.) of the respective raw materials may be used as they are, and a dried product obtained by drying these or a powder obtained by pulverizing after drying can also be used. In addition, these may be extracts or powders obtained by combining known techniques such as solvent extraction, pressing, enzymatic decomposition, supercritical extraction, concentration, dilution, solid-liquid separation, and purification alone or in combination.

  At this time, solvent extraction is mentioned as a preferable method. Examples of the solvent used include water, methanol, ethanol, isopropanol, acetone, 1,3-butylene glycol, ethylene glycol, propylene glycol, glycerin, acetic acid, ethyl acetate, ether, hexane, and a mixed solvent thereof. Of these, water, ethanol and an aqueous ethanol solution (ethanol concentration: 10 to 90%) are particularly preferable.

  The amount of the solvent to be used is not particularly limited, but is usually 0.5 to 50 parts by weight, preferably 1.0 to 30 parts by weight, and particularly preferably 5.0 to 1 part by weight of the dried plant. Used at 20 parts by mass. When the amount of the solvent is less than 0.5 with respect to 1 part by mass of the dried plant body, extraction may not be sufficient depending on the type of the solvent, and when the amount of the solvent exceeds 50 parts by mass, it is not economically advantageous. There is.

  As an extraction method, it can be obtained as it is or by immersing the pulverized product in a suitable extraction solvent, a method of stirring under heating (room temperature to the boiling point of the solvent), or the like. For example, a method of obtaining an extract by stirring a plant pulverized product in 50% ethanol at room temperature for 10 to 60 minutes, or extracting a plant pulverized product by stirring in water at 100 ° C. under reflux for 30 to 60 minutes The method of obtaining a thing etc. are mentioned.

  The extract obtained by the above extraction operation may remove lavender aroma components such as linalool and cineol. Methods for removing aroma components from the extract include solvent extraction (eg, US Pat. No. 3,950,266), supercritical carbon dioxide (eg, JP-A-3-9985), and synthetic adsorption resin. A method to be used (for example, JP-A No. 2003-105337) can be used alone or in appropriate combination.

  Further, it may be a pophenol-containing fraction obtained by fractionating the extract. The fractionation method is particularly preferably fractionation with a synthetic adsorbent. Fractionation with a synthetic adsorbent may be performed by a conventional method, for example, a method in which a solution containing the extract is brought into contact with a synthetic adsorbent packed in a column at a constant flow rate, There is a method in which a synthetic adsorbent is added to an extract-containing liquid and the synthetic adsorbent is separated after stirring for a certain time. There is no particular restriction on the method, and it can be selected according to the purpose.

  The polyphenol component adsorbed on the synthetic adsorbent can be eluted with various organic solvents, for example, organic solvents such as methanol, ethanol, 2-propanol, and acetone, or these aqueous solutions as necessary. The solvent used for elution is most preferably ethanol or an aqueous solution thereof from the viewpoint of safety to the human body and handleability.

  The synthetic adsorbent to be used is generally an insoluble three-dimensional cross-linked polymer and has substantially no functional group such as an ion exchange group. (Trademark) XAD-16 "(manufactured by Organo Corporation)," Separbeads SP70 (trade name) "(Mitsubishi Chemical Corporation) or" Diaion HP20 (trade name) "(Mitsubishi Chemical), which is a styrene-divinylbenzene copolymer system Can be used, but is not limited thereto.

(2) Anti-stress agent-containing composition In the anti-stress agent of the present invention, an anti-stress agent-containing composition can be prepared by containing other optional components usually used for foods and drinks or for medicines. .
Optional ingredients used include, for example, sweeteners, colorants, preservatives, thickening stabilizers, antioxidants, bittering agents, acidulants, emulsifiers, reinforcing agents, manufacturing agents, excipients, disintegrants, binders , Lubricants, coating agents, plasticizers, fragrances and the like, and these can be added to be used as various preparations and dosage forms.

  Examples of the fragrance that can be further used in the present invention include ethyl acetoacetate, acetophenone, anisaldehyde, α-amylcinnamaldehyde, methyl anthranilate, ionone, isoeugenol, isoamyl isovalerate, ethyl isovalerate, isothiocyanic acid. Allyl, 3-butenyl isothiocyanate,

4-pentenyl isothiocyanate, benzyl isothiocyanate, 3-methylthiopropyl isothiocyanate, isothiocyanates, indole and its derivatives, γ-undecalactone, esters, ethyl vanillin, ethers, eugenol, octanol, octanal, ethyl octoate , Isoamyl formate, geranyl formate, citronellyl formate, cinnamic acid, ethyl cinnamate, methyl cinnamate, ketones, geraniol, isoamyl acetate, ethyl acetate, geranyl acetate, cyclohexyl acetate, citronellyl acetate, cinnamyl acetate, terpinyl acetate, Phenethyl acetate, butyl acetate, benzyl acetate, l-menthyl acetate,

Linalyl acetate, methyl salicylate, allyl cyclohexylpropionate, citral, citronellal, citronellol, 1,8-cineole, fatty acids, higher aliphatic alcohols, higher aliphatic aldehydes, higher aliphatic hydrocarbons, cinnamyl alcohol, cinna Maldehyde, thioethers, thiols, decanal, decanol, ethyl decanoate, terpineol, limonene, pinene, myrcene, tapinolen, terpene hydrocarbons, γ-nonalactone, vanillin, paramethylacetophenone, hydroxycitronellal, hydroxycitronone Lar dimethyl acetal, piperonal, isoamyl phenyl acetate, isobutyl phenyl acetate, ethyl phenyl acetate, phenol ethers,

Phenols, furfural and its derivatives, propionic acid, isoamyl propionate, ethyl propionate, benzyl propionate, hexanoic acid, allyl hexanoate, ethyl hexanoate, ethyl heptanoate, l-perilaldehyde, benzyl alcohol, benzaldehyde, aromatic Alcohols, aromatic aldehydes, d-borneol, maltol, methyl N-methylanthranilate, methyl β-naphthyl ketone, dl-menthol, butyric acid, isoamyl butyrate, ethyl butyrate, cyclohexyl butyrate, butyl butyrate, lactones, linalool, etc. Citrus essential oils such as orange, lemon, lime, grapefruit, fruit, such as apple, banana, grape, melon, peach, pineapple, strawberry Essential oils or recovered flavor,

Milk-based extract flavors such as milk, cream, butter, cheese, yogurt, green tea, oolong tea, tea, coffee, cocoa, etc.Recovery flavor, asanomi, asafetida, ajowan, anise, angelica, fennel, turmeric, oregano, allspice , Orange nope, pepper, cassia, chamomile, mustard, cardamom, curry leaf, licorice, caraway, gardenia, cumin, watercress, clove, poppy flea, caper, pepper, sesame, coriander, sassafras, saffron, savory, salvia, sun show,

Cinnamon, shallot, juniper berry, ginger, star anise, horseradish, celery, sorrel, thyme, onion, tamarind, tarragon, chives, dill, pepper, nutmeg, sagebrush, nigera, carrot, garlic, basil, parsley, vanilla, paprika , Hyssop, Fenegreek, Horseradish, Marjoram, Myoga, Lavender, Linden, Lemongrass, Lemon Balm, Rose, Rosemary, Laurel, Wasabi, etc.

Asafetida, Asian Tam, Ajowan, Azuki, Asparagus Linea Alice, Apple Mint, Artichoke, Anise, Avocado, Achacha, Amazazuru, Amiga Sayuri, Amiris, Almond, Aritasou, Alcanna, Artemisia, Arnica, Alfalfa, Aloe, Angoslaan , Chanterelles, angelica, amber, ambergris, amblet, squid, licorice, rush, yeast, itadori, strawberries, figs, ginkgo, mushrooms, ylang ylang, sardine, impeller tria, immortel, winter green, watercress, sea cucumber, turmeric , Usbasaishin, Woodruff, Sea Urchin, Ume, Oolongcha, Egoma, Enokidake, Shrimp, Shrimp, Eligeron, Elda , Eleutherococcus, Erekanpen, elemi, Corydalis, Sophora, endive, Europe thistle, Coptis,

Psyllium, Okazeri, Krill, Oak, Oak Moss, Okera, Osmanthus, Opoponax, Ominaeshi, Omodaka, Dutch Sennichi, Origanum, Oris, Orynamum, Olive, Allspice, Orange, Orange Flower, Kai, Kainin, Kakao, Oyster, Kasai, Cashew nut, Cascara, Cascarilla, Castrium, Katakuri, Cutlet obsi, Cassie, Cassia fistula, catechu, crab, carnation, valerian, camomil,

Kayapute, mustard, crow uri, crow birch, guarana, calamus, galanga, currant, carissa, karin, cardamom, galvanum, curry, kawamidori, licorice, ganbia, kanran, kiwifruit, kikaigaratake, kyoto, chrysanthemum, chrysanthemum, scallop , Kina, Yellowfin, Hosta, Gymnema Sylvestre, Catnip, Caraway, Carop, Cucumber, Kiraya, Kinkihizuki, Guava, Guayak, Kuko, Kusasagi Kazura, Kuboke, Kuzu, Kusunoki, Kusunohagasiwa, Gooseberry,

Gardenia, Kubeba, Bearberry, Gummy, Cumin, Ground Ivy, Clara, Clarisage, Cranberry, Chestnut, Walnut, Cream, Grain of Paradise, Cretadi Tanny, Grapefruit, Clover, Clove, Black Moji, Chlorella, Mulberry, Quasher, Caper, Ghetto , Cade, Kebraco, Germander, Kentur, Kemponashi, Gennoshouko, Kouji, Kodatake, Koucha, Kohone, Coca, Koganebana, Kokuto, Kokului, Coconut, Goshuyu, Pepper, Costas, Costmary, Copaipa, Coffee, Kobushi, Burdock, Sesame, cola, coriander, coltsfoot, golden rod, colombo, consai, conzulango, confrey, cypress,

Fish, Sakura, Cherry, Pomegranate, Salmon Cass, Sasa, Sasakusa, Search, Sassafras, Saffron, Sapodilla, Cactus, Sarasinashouma, Salsaparilla, Salsify, Sarnosica, Hawthorn, Sanshuyu, Salamander, Santa Herb, Sandalak, Sandalwood, Sandal Red, Shiitake, Gene, Cedar, Citrus, Citronella, Cinus, Civet, Shimaruba, Shimji, Peonies, Jasmine, Janohige, Jaborangi, Shallot, Shukusha, Juniper Berry, Ginger, Shoyu, Shoyukas, Zhouryusshu, Shoro, Shirotamogi Ginseng, cinnamon, vinegar, watermelon, narcissus, cedar, star anise, star fruit, stylax, suppon, suptake bamboo, zudravets, snake crew , Spikenard, spruce,

Purslane, slow berry, savory, buffalo, sage, zedary, senega, geranium, celery, nematode, centauria, sengen, st jones wort, senna, sauce, diau, soy, thyme, bamboo shoot, octopus, tade, dabana, egg, tamagotake , Onion, tamarind, Damiana, tamogitake, tarragon, taranoki, tansy, tangerine, dandelion, cherimola, cherry laurel, cherry wild, chigaya, chicory, cheese, chichitake, chives, chervil, champaca, tuberose, datura, chillata, tsukushi, Tsukumono, ivy, camellia, camellia, crayfish, carrot, dartang, thistle, ditany, dill,

Dates, Tendai Yaku, Tenma, Pepper, Toki, Doushokbutsu Protein Sat, Doushokubutsuyu, Tomitsu, Corn, Dokudami, Tochu, Doggrass, Tomato, Dragon Blood, Durian, Truffle, Truval Sam, Tonka, Naginata Koju, Pear, Naster Siamese , Nuts, natto, jujube, nutmeg, radish, sea cucumber, larvae, niari, freshwater cinnamon, carrots, garlic, rat potato, nettle, leeks, knotgrass, violet, pineapple, hibiscus, malt, chickweed, basil, lotus, lotus cup , Perth cup, parsley, butter, butter oil, buttermilk, birch, honey, patchouli, buck bean, kkoshu, kkonoyu, kkomieki, pa Deployment fruit, Hatsutake, buffalo berry, pearl barley, Anemarrhenae asphodeloides, banana, vanilla, Hanesakkuru,

Papaya, barberry, clam, hamasuge, hamanasu, hamabofu, hamamelis, rose, palmarosa, panda, van lychee, toad, cypress, pistachio, hyssop, hickory, peanuts, cypress, hiba, pipsiwa, himehagi, hyacinth, hiratake, hiratake, hirabin, hinata Feijoa, Fenegreek, Fennel, Fujibakama, Fujimodoki, Fusuma, Fuselyu, Petit Grain, Buchu, Grapes, Grape Salmon Cass, Peach, Beech, Beech Haritake, Black Caraway, Blackberry, Plum, Briononia, Prickly Ash, Primrose, Prunella, Blueberries, breadfruit,

Hay, bay, hazelnut, vetiver, betel, safflower, penny royal, snake, pepino, peptone, bergamot, bergamot mint, peruvian balsam, velvena, veronica, benzoin, boadrose, hoahound, hoe, broomtake, scallop, bowfish, whey, hoonoki , Horseradish, button, hop, poppy, poplar, poppo, jojoba, squirt, bordeaux, bologna, maitake, mawwalt, marshmallow, marjoram, mastic, masui, matabobi, machiko, pine, pine mushroom, mushroom, matsutake, matsubusa, Matsuhodo, matecha, bean, marigold, marubadao, quince, malein, mallow, mango, mangosteen, mandarin orange, mishima psycho, miso, mitsumata, beeswax, mi Door, mimosa, Zingiber mioga, milk, myrtle, Mirufoiru, myrrh, Mirobaran, barley tea, musk, purple, Mesquite, Meadow sweet, Mehajiki, maple, Melissa, MERIROTTO,

Melon, Mosengoke, Moniria Bayoueki, Mominoki, Peach, Moroheiya, Yakuchi, Yamabomo, Eucalyptus, Yukinoshita, Yuzu, Yucca, Lily, Yosai, Yorusa, Lions Foot, Lychee, Life Everlasting Flower, Lime, Lilac, Lacanka, Lacan Show, raspberry, latania, radish, lovedanum, lavender, langwald, langmoth,

Rambutan, Liqueur, Leak, Lyzea, Linaroe, Longan, Ryofunsou, Ryokucha, Apple, Linden, Gentian, Lou, Borage, Reseda, Lemon, Lemongrass, Forsythia, Astragalus, Remb, Rosemary, Lobage, Laurel, Longosa,
Natural fragrances obtained from horseradish, cotton foliage, wormwood, worm seed, bracken, bracken, etc. are exemplified, and are selected and used as appropriate.

  The amount of the fragrance added is not particularly limited, but is generally 0.0001 to 50% by mass, preferably 0.001 to 30% by mass, most preferably in the composition containing the anti-stress agent of the present invention. Is blended so that the added amount is 0.01 to 10% by mass. The antistress agent of the present invention can be used as it is or in a diluted state, an emulsified state, and various powdered preparations.

(3) Food / beverage products containing an anti-stress agent In order to manufacture food / beverage products containing the anti-stress agent according to the present invention, the component produced by the above method or its preparation can be used, and conventional means are used. In addition, edible state, for example, granules, granules, tablets, capsules, soft capsules, pastes and the like can be used.

  This food and drink may be used for food as it is, and used by adding it to various foods (for example, ham, sausage, kamaboko, chikuwa, bread, butter, powdered milk, confectionery, etc.) or water, alcoholic beverages, fruit juice, milk It may be used by adding to beverages such as soft drinks.

(4) Drug containing anti-stress agent When the anti-stress agent of the present invention is used as a drug, it can be used in various dosage forms as it is, but preferably a tablet, pill, powder, syrup, emulsion, It is used as an internal medicine formulated as a liquid, capsule, or injection.

  The intake of the anti-stress agent of the present invention is appropriately selected and determined according to age, weight, symptoms, degree of disease, form of medicine or food / drink, etc., for example, 1 mg to 10 g per day as an extracted and dried powder The amount is preferably 10 mg to 1 g, and may be taken in several divided doses a day.

  EXAMPLES Hereinafter, although an Example etc. are given and this invention is further demonstrated, this invention is not limited to these.

[Production Example 1]
1 kg of dried flowers of true lavender was immersed in 20 kg of hot water at 90 ° C. and extracted by stirring at 90 ° C. for 1 hour. After cooling, the residue obtained by filtering off the residue was concentrated to dryness to obtain 250 g of extract. In addition, the aromatic component does not remain in the obtained extract.
The physical properties of the obtained extract were as follows.
a) Ultraviolet absorption spectrum: as shown in FIG. 1 (measurement concentration 200 ppm, diluting solvent: distilled water); λmax: 206 nm, 275 nm; use “Spectrophotometer UV-2450” manufactured by Shimadzu Corporation did.
b) Solubility: Easily soluble in water, soluble in 50% ethanol, hardly soluble in ethanol.

[Production Example 2]
20 kg of 50% ethanol was added to 1 kg of crushed dried flowers of true lavender, and the mixture was immersed and extracted at room temperature for 10 minutes. Distilled water was added to the extract obtained by filtering off the residue to adjust the ethanol concentration to 30%, and a column (diameter 135 mm) packed with a synthetic adsorbent “Separbeads SP70 (trade name)” manufactured by Mitsubishi Chemical Corporation. X length 120 cm). Further, the polyphenol-containing fraction was eluted by flowing 30% ethanol through the column. The resulting eluate was concentrated to dryness to obtain 226 g of a polyphenol-containing fraction. In addition, the aromatic component does not remain in the polyphenol-containing fraction.
The physical properties of the obtained polyphenol-containing fraction were as follows.
a) Ultraviolet absorption spectrum: as shown in FIG. 2 (measurement concentration 200 ppm, diluting solvent: distilled water); λmax: 206 nm, 275 nm; use “Spectrophotometer UV-2450” manufactured by Shimadzu Corporation did.
b) Solubility: Easily soluble in water, easily soluble in 50% ethanol, slightly soluble in ethanol.

[Production Example 3]
Using dried spike lavender flowers, the same treatment as in Production Example 1 was performed to obtain 245 g of extract. In addition, the aromatic component does not remain in the obtained extract.

[Production Example 4]
Using 1 kg of lavandin dried flower pulverized product, the same treatment as in Production Example 2 was performed to obtain 223 g of a polyphenol-containing fraction. In addition, the aromatic component does not remain in the polyphenol-containing fraction.

[Test example]
The effect of the anti-stress agent of the present invention was examined in an animal model of stress gastric ulcer. In this study, mice are subjected to psychological and physical stress due to forced swimming, and the area of the gastric ulcer that develops is measured to evaluate the anti-stress effect of the test substance.

(Test method)
Slc: ICR mice fasted for 24 hours (manufactured by SLC Japan, 6 weeks old, male) were divided into 5 groups with 9 mice per group, and the anti-stress agent of Production Example 2 dissolved in distilled water was added to 1 kg of mouse body weight. It was administered by oral gavage at doses of 500, 1000, and 2000 mg per unit. No abnormality was observed in the behavior of the mice after administration. Only the distilled water was administered to the control group. The group without swimming was administered distilled water 2 hours before gastrectomy.

One hour after administration, the mouse was floated on a forced swimming additional device that generated a water flow (water temperature 25 ° C., flow rate 5 L / min), and forced swimming was loaded for 1 hour. As a forced swimming additional device, a Kyoto Omatsumoto style momentum measuring water tank (Anitech Co., Ltd.) was used.
After completion of swimming, blood was removed under ether anesthesia to euthanize the mouse, and the stomach was removed and fixed with 10% neutral buffered formalin solution. Thereafter, the area of the damaged stomach was measured using a stereomicroscope.

  The average value and standard error of the gastric ulcer area of each test group were calculated, and further, the difference between the average values of the non-swimming group and the control group was determined by Student's t-test. Differences in group mean values were analyzed by Dunnett's multiple comparison test.

As a comparative example, the effects of R-(-)-linalool (Comparative Example 1) and 1,8-cineol (Comparative Example 2), which are essential oil components of Labiatae plant, on the stress gastric ulcer are as described above. A similar method was examined.
However, the administration medium was not distilled water, but 1% nonionic surfactant “Tween 80” (trade name) aqueous solution.
The dose was set at 100 mg / kg mouse body weight because, when 500 mg or more per kg body weight of the mouse was administered, an extreme decrease in motor activity was observed as a side effect in both essential oil components after administration. The control group received 1% Tween 80 aqueous solution.

(Test results)
The effects of oral administration of the antistress agent of Production Example 2 and the essential oil component of Comparative Example on the area of gastric ulcer are shown in FIGS. 1 and 2, respectively. When the antistress agent of Production Example 2 was administered, the gastric ulcer area was significantly reduced compared to the control group at all doses studied. On the other hand, when the essential oil component of the comparative example was administered, there was no significant difference in the gastric ulcer area compared to the control group.

[Example 1] (mixed tea beverage)
To 500 ml of hot water at 90 ° C, add 1 g of green tea leaves, 8 g of hatomugi, 1 g of barley, 0.2 g of brown rice, 0.2 g of puer tea, 0.1 g of dokudami tea, 0.1 g of habucha and 0.1 g of chicory. For 8 minutes. After extraction, solid-liquid separation is performed, and 0.1 g of vitamin C and water are added to make 10000 ml. After adjusting the pH to 5.5 with sodium hydrogen carbonate, the antistress agent of Production Example 2 is adjusted to 0.1%. Addition to obtain a mixed tea beverage.

[Example 2] (Soft drink)
Add 30 mg of Valencia orange juice, 3 ml of lemon juice, 1.5 g of fructose, 0.5 g of citric acid, 100 mg of vitamin C, add 100 mg of the anti-stress agent of Production Example 2, add water to 100 ml, stir well, and then add carbon dioxide gas Got a soft drink.

[Example 3] (chewing gum)
100 g of sugar, 0.5 g of fragrance, and 30 g of 1% aqueous solution of the antistress agent of Production Example 1 were added to 50 g of gum base, kneaded using a kneader, and completed after molding.

[Example 4] (Biscuits)
100 g of strong powder, 100 g of shortening, 40 g of super white sugar, 30 g of weak flour, 20 g of water, 10 g of the antistress agent of Production Example 3, 4 g of full fat powdered milk, and 0.6 g of baking soda were molded and baked to obtain biscuits.

[Example 5] (Candy)
After mixing 280 g of starch syrup, 360 g of granulated sugar, and 120 g of the antistress agent of Production Example 4, the mixture was heated to 155 ° C. Then, it cooled to 120 degreeC, citric acid 12g, the fragrance | flavor 1.2g, and glycerol 50g were added, and it completed after shaping | molding and cooling.

[Example 6] (Granule)
5.0 g of lactose and 5.0 g of corn starch were added to 5.0 g of the antistress agent of Production Example 2 and kneaded, granulated, dried and sized.

[Example 7] (Capsule)
After thoroughly mixing 5.0 g of the antistress agent of Production Example 1, 5.0 g of corn starch, 5.0 g of lactose, and 1.0 g of crystalline cellulose, the capsules were filled into 40 capsules.

[Example 8] (Tablet)
After thoroughly mixing 2.0 g of corn starch, 50 g of lactose, 0.2 g of calcium stearate, and 1.8 g of talc with 50 g of the antistress agent of Production Example 2, the tablet is compressed by a tableting machine to obtain a tablet having a weight of 0.52 g. 200 tablets were produced.

  INDUSTRIAL APPLICABILITY According to the present invention, it is possible to provide a safe anti-stress agent that can effectively prevent or reduce various symptoms caused by stress and has no side effects or habits, and a food, beverage, medicine, or fragrance composition containing the same. Therefore, the present invention is extremely useful industrially.

Claims (8)

  An anti-stress agent containing a plant belonging to the genus Lavandula as an active ingredient.   An anti-stress agent comprising an extract of a plant of the genus Lavandula as an active ingredient.   An anti-stress agent comprising, as an active ingredient, a polyphenol-containing fraction obtained by fractionating an extract of a genus Lavandula.   Labiatae Lavandula lavender (Lavandula angustifolia), spike lavender (Lavandula latifolia), Wool lavender (Lavandula lanta), lavandin (Lavandula x intermedia), vesta lavand ), Willidis lavender (Lavandula viridis), Dentara lavender (Lavandula dentata), Multivida lavender (Lavandula multifida) and Pinata lavender (Lavandula pinnata) and True lavender At least one selected from the group consisting of a cross of Lavandula angustifolia and Woolley lavender, and a cross of Lavandula lavender and Lavandula viridis 4. The antistress agent according to any one of items 3.   The antistress agent according to any one of claims 1 to 4, for preventing or ameliorating stress gastric ulcer and duodenal ulcer and stress gastritis.   An anti-stress food or drink comprising the anti-stress agent according to any one of claims 1 to 5.   An antistress pharmaceutical comprising the antistress agent according to any one of claims 1 to 5.   An anti-stress perfume composition comprising the anti-stress agent according to any one of claims 1 to 5.

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