JP2010024211A - Cell proliferation promoting agent - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a cell proliferation promoting agent having high cell proliferation promoting action. <P>SOLUTION: Provided are a cell proliferation promoting agent comprising an extract of a plant belonging to the genus Psophocarpus, family Leguminosae, a TGF-β production promoting agent comprising an extract of Psophocarpus tetragonolobus, a collagen gel constriction promoting agent and an integrin production promoting agent. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to a cell growth promoter, and more particularly to a cell growth promoter having an action of promoting extremely high cell growth.

  The epidermis and dermis of the skin are composed of epidermal cells, fibroblasts and an extracellular matrix such as collagen that is outside these cells and supports the skin structure. Above all, in younger skin, fibroblasts are actively proliferating, and these skin tissue interactions maintain homeostasis, ensuring moisture retention, flexibility, elasticity, etc. It is kept fresh with tension and gloss.

  However, when there is an influence of certain external factors such as ultraviolet irradiation, drastic air drying, excessive skin washing, etc., or when aging progresses, senile appears on the skin and fibroblast proliferation ability also increases. descend. As a result, there is a problem that the skin moisturizing function and elasticity are lowered, the exfoliation of the keratin begins to be abnormally peeled, the skin loses its tension and gloss, and exhibits aging symptoms such as roughness and wrinkles.

Thus, it is known that a decrease in the proliferative ability of fibroblasts is deeply involved in changes accompanying skin aging, that is, wrinkles, dullness, disappearance of texture, and decrease in elasticity.
For this reason, various proposals have been made on natural cell growth promoters that promote the proliferation of fibroblasts, have the effect of preventing skin aging and improving skin aging, and are highly safe. For example, an extract from a leaf portion of Averhoa carambola L. (for example, see Patent Document 1), an extract of lotus germ (for example, see Patent Document 2), pigmented rice, or rice with pigmented rice Extracts (for example, see Patent Document 3), extracts of maple genus maple (for example, see Patent Document 4), extracts such as aloe vera, almond, enokitake (for example, see Patent Document 5), kiyonin, passion flower, Extracts of plants such as licorice, merirot and echinacea (for example, see Patent Document 6) have been proposed.
In addition, the ability to promote cell growth is useful for arthritis prevention treatment, initial treatment for burns, and the like, and can be used for various purposes other than application to an external skin preparation for anti-aging purposes.

JP 2002-226323 A JP 2002-29980 A JP 2002-3393 A JP 2003-1113068 A JP 2003-104835 A JP 2003-34631 A

  However, a specific compound that promotes fibroblast proliferation in the extract has not yet been specified, and there is a need to provide a natural fibroblast proliferation promoter having a higher fibroblast proliferation promoting effect. The current situation is strongly desired.

  The present invention has been made in response to the conventional circumstances as described above, and an object thereof is to provide a cell growth promoting agent having a high cell growth promoting action.

The present invention is a cell growth promoter comprising an extract of one or more plants selected from plants belonging to the genus Psophocarpus.
Further, according to the present invention, a TGF-β production promoter, collagen gel contraction, comprising an extract of one or more plants selected from plants belonging to the genus Psophocarpus. Accelerators and integrin production promoters are provided.

Here, TGF-β is a type of cytokine (a secreted protein that regulates cell functions) that controls cell proliferation and differentiation and maintains homeostasis, and is expressed in almost all organs and tissues of the living body. It is what.
That is, TGF-β is closely involved in cell differentiation / migration / adhesion, and plays an important role in a wide range of areas such as ontogeny, tissue reconstruction, wound healing, inflammation / immunity, cancer invasion / metastasis. In cancers of digestive organs such as the large intestine, pancreas, and stomach, and cancers such as leukemia, functional abnormalities have been reported due to gene mutations in TGF-β, TGF-β receptors, and TGF-β signaling molecules (Smad). Cell growth control (suppression) by TGF-β is an important physiological brake in human cells, but failure of the brake is thought to lead to an abnormal cell growth reaction, that is, canceration. .
It has also been pointed out that TGF-β may suppress the onset of allergic diseases and infections in infants such as atopic dermatitis and asthma (Kalliomaki M et al.: J Allergy Clin Immunol 104 (6): 1251). -7, 1999, Oddy WH et al .: J Allergy Clin Immunol 112 (4): 723-8, 2003.).
Furthermore, it is known that allergic reaction can be selectively suppressed by oral administration of TGF-β (Japanese Patent Application No. 2005-252912, Okamoto A et al .: Int Immunol. 17 (6): 705-12, 2005. ).

  Collagen gel contraction is considered to be a model system for wound healing, and the degree of collagen gel contraction reflects the motor activity of cells and is promoted by various cell growth factors and cytokines. Therefore, the collagen gel contraction promoter can be used as a drug for promoting the fixation of a graft in the treatment of intractable skin diseases such as wound healing, skin ulcer and keloid, or in skin transplantation.

Integrin is one of cell surface proteins and is a cell adhesion molecule mainly involved in cell adhesion to the extracellular matrix and signal transmission from the extracellular matrix. It is a heterodimer composed of two subunits, α chain and β chain.
Integrins bind to ligands of fibronectin and laminin, which are extracellular matrix components, and are involved in cell adhesion and migration. Therefore, increasing integrin production is expected to work as a tissue structure stabilizer and wound healing promoter. It also binds directly or indirectly to various signal transduction factors and actin-binding proteins in the intracellular region, and plays a role in transmitting information written in the extracellular matrix into the cell.
The integrin family is also involved when normalizing by platelet aggregation during vascular injury, or when leukocytes migrate to and invade extravascular tissues, destroying damaged tissues or phagocytosing foreign bodies. Yes.

The cell growth promoting agent of the present invention is excellent in cell growth promoting action. In addition, the cell growth promoter of the present invention can be applied to, for example, basic cosmetics, medicinal cosmetics, pharmaceutical preparations, and the like, and is highly safe with significantly improved cell growth promoting effects.
The TGF-β production promoter of the present invention can maintain cell homeostasis by controlling cell proliferation / differentiation by promoting TGF-β production.
The collagen gel contraction-promoting agent of the present invention promotes the contraction of the collagen gel, thereby promoting the fixation of the graft in the treatment of intractable skin diseases such as wound healing, skin ulcer and keloid, or skin transplantation. it can.
The integrin production promoter of the present invention can stabilize tissue structure and promote wound healing by promoting integrin production.

Hereinafter, the present invention will be described in detail.
The plant belonging to the genus Psophocarpus used in the present invention is a tropical vine of the leguminous family. In this invention, if it is a plant which belongs to the winged genus, it will not specifically limit, It can use arbitrarily. In the present invention, winged bean (scientific name: Psophocarpus tetragonolobus) is preferably used. The winged bean is also called a winged bean. “Urisun” is known as an improved variety. Commercially available products can be purchased from Sakata Seeds.
The winged bean extract is conventionally known as a component having a whitening action, a moisturizing action, and a laminin 5 production promoting action (JP 2002-265343 A, JP 2002-265324 A, JP 2003-313135 A). It is not known to have a cell growth promoting action, and was first discovered by the present inventor.

  The extract of the plant belonging to the genus Psophocarpus used in the present invention is obtained by immersing or heating leaves, stems, branches, flowers, bark, seeds, fruits, rhizomes or whole plants of plants of the genus Psophocarpus with an extraction solvent. After refluxing, it is obtained by filtration and concentration. Although any part of the plant can be used as the part to be used, it is particularly preferable to use seeds.

  An extract of a genus Psophocarpus can be obtained by a conventional method. For example, a genus Psophocarpus can be obtained by dipping or heating under reflux with an extraction solvent, followed by filtration and concentration. As the extraction solvent, any solvent that is usually used for extraction can be used. For example, water, methanol, ethanol, propylene glycol, 1,3-butylene glycol, glycerin and other alcohols, hydrous alcohols, chloroform , Organic solvents such as dichloroethane, carbon tetrachloride, acetone, ethyl acetate, hexane and the like can be used alone or in combination. The extract obtained by extraction with the above solvent is adsorbed as it is, or the concentrated extract is adsorbed by an adsorption method, for example, by removing impurities using an ion exchange resin, or by a column of a porous polymer (eg Amberlite XAD-2). Thereafter, a product eluted with methanol or ethanol and concentrated can also be used. Further, a partitioning method, for example, an extract extracted with water / ethyl acetate can be used.

  The extract thus obtained can be used as it is, or further diluted with ethanol or the like, or solidified, and then the dried product can be used as it is, or the dried product can be redissolved in, for example, ethanol to obtain the preparation used in the present invention.

  The extract of the genus Psophocarpus thus obtained has an excellent cell growth promoting action. Such a cell growth promoter composed of an extract of the plant of the genus Psophocarpus is preferably used in a preparation for external use or food.

  Moreover, since the cell growth promoting agent of the present invention has excellent cell growth promoting ability and cell adhesion promoting ability, it can be used as, for example, a medium additive in addition to a skin external preparation or food. Conventionally, as a culture medium additive, animal-derived materials such as bovine pituitary extract and fetal bovine serum have been used, but use of winged bean extract makes it possible to use it as a non-animal material. The cell growth promoter of the present invention can also be used in regenerative medicine, can be used for artificial skin and self-cultured skin preparation, and is a non-animal raw material, so it is highly valuable in terms of safety. is there. It can also be applied as an arthritis prevention treatment or an initial treatment for burns.

  Here, the cell is not particularly limited, and examples thereof include mammalian cells. Mammalian cells are tissue, organ cells or cells derived from mammals such as humans, monkeys, cows, rats, mice, etc., which are cells of individuals, primary cells removed from individuals, cultured cells, and genes. Any of the introduced cells may be used. The cell type is not particularly limited, and is derived from ectoderm (skin epidermis, nerve, etc.), endoderm (digestive tract, organ tissue, etc.), mesoderm (skin dermis, connective tissue, heart / blood vessel, muscle) Etc.) and neural crest-derived (peripheral nerve etc.).

  As an administration method, in the case of an in vitro method or an ex vivo method, it may be added to a culture medium in which cells are cultured or added directly to cells. The amount added can be appropriately adjusted depending on the type of cells, the number of cells, etc., but it is sufficient that no cell toxicity is observed and cell growth promoting activity is recognized.

  When the cell growth promoter of the present invention is used by blending with a skin external preparation, it is preferably blended in a dry skin weight of 0.0001 to 10 mass%, more preferably 0.001 to 1 mass in the total amount of the skin external preparation. %. If it is less than 0.0001% by mass, the cell growth promoting action of the present invention is not sufficiently exerted. On the other hand, if it exceeds 10% by mass, it is difficult to make a preparation. Moreover, even if it exceeds 1 mass%, the improvement of the big effect is not recognized.

  When the cell growth promoter of the present invention is used for an external preparation for skin, in addition to the above components, components that are usually used for external preparations for skin such as cosmetics and pharmaceuticals, as long as they do not impair the effects of the present invention, For example, moisturizers, antioxidants, oils, UV protection agents, surfactants, thickeners, alcohols, powder components, color materials, aqueous components, water, various skin nutrients, etc., should be added as necessary. Can do.

  Furthermore, edetate disodium, edetate trisodium, sodium citrate, sodium polyphosphate, sodium metaphosphate, sequestering agents such as gluconic acid, preservatives such as methylparaben, ethylparaben, butylparaben, caffeine, tannin, Verapamil, tranexamic acid and its derivatives, licorice extract, grabrizine, hot water extract of karin fruit, various herbal medicines, tocopherol acetate, glycyrrhizic acid and its derivatives or salts thereof, vitamin C, magnesium ascorbate phosphate, Whitening agents such as ascorbic acid glucoside, arbutin, kojic acid, sugars such as glucose, fructose, mannose, sucrose, trehalose, vitamin A derivatives such as retinoic acid, retinol, retinol acetic acid, retinol palmitic acid It may be blended Domo.

  Further, the external preparation for skin containing the cell growth promoter of the present invention can be widely applied to cosmetics, quasi-drugs and the like, particularly preferably cosmetics applied to the outer skin, and the dosage form is also Any one can be used as long as it is applicable to the skin, solution system, solubilization system, emulsification system, powder dispersion system, water-oil two-layer system, water-oil-powder three-layer system, ointment, lotion, gel, Any dosage form such as aerosol is applied.

  The use form of the external preparation for skin containing the cell growth promoter of the present invention is also arbitrary, for example, facial cosmetics such as lotion, milky lotion, cream and pack, cosmetics for body such as sunscreen and massage oil, and foundations. It can be used in makeup cosmetics such as lipsticks and eye shadows, hair cosmetics, aromatic cosmetics, bath preparations and the like.

When the winged genus plant extract is used in a food or pharmaceutical preparation, in addition to the above components, if necessary, within the range not impairing the effects of the present invention, for example, granular, granular, pasty, gel-like It can be arbitrarily molded into a solid or liquid form. These include various known substances that are recognized to be contained in foods and drinks, such as binders, disintegrants, thickeners, dispersants, reabsorption accelerators, flavoring agents, buffers, and surfactants. , Solubilizers, preservatives, emulsifiers, isotonic agents, excipients such as stabilizers and pH adjusters, and the like can be appropriately blended.
The dosage form is also arbitrary and can be appropriately adjusted, for example, tablets, granules, powders, capsules and the like.

  In addition, the form which the skin external preparation containing the cell growth promoter of this invention, a foodstuff, and a pharmaceutical formulation can take in said dosage form and usage form is not limited.

  EXAMPLES Next, although an Example is given and this invention is demonstrated further in detail, the technical scope of this invention is not limited at all by this.

1. Preparation of sample (plant extract) 50 g of winged bean seeds were immersed in 90% ethanol for 1 week at room temperature, and the extract was concentrated to obtain 2.9 g of 90% ethanol extract (dry product). The following experiment was performed using the obtained winged bean extract.

2. Measurement of Fibroblast Growth Promoting Activity A 96-well plate was seeded with 5,000 human skin fibroblasts using DMEM containing 10% FBS, and after adhesion, the medium was replaced with a medium containing 0.5% FBS. The next day, winged bean extract dissolved in DMSO was added at 10 ⁻⁴ to 10 ⁻² mass% (DMSO final concentration 0.5%). After further cultivation for 72 hours, the medium was collected. A Hoechst 33342 (Hoechst) solution was added to the plate and subjected to DNA staining. Then, the fluorescence was measured, and the cell proliferation rate was determined from the ratio to the drug-free (control) group. The results are shown in Table 1.
However, the same result was obtained for any cell type in this example.

  From Table 1, it was found that winged bean extract has an excellent growth promoting effect on fibroblasts.

3. Measurement of TGF-β1 production promoting action HaCaT cells were seeded in a 10 cm petri dish and cultured in a KGM medium (manufactured by Kurabo Industries Inc.) until it increased to about 70% of the whole petri dish. Add 0.0002 wt%, 0.001 wt%, and 0.005 wt% winged bean extract that was replaced with 0.1% BSA-containing KBM medium and simultaneously dissolved in DMSO (DMSO final concentration 0.5%) The culture was further performed for 48 hours.
After the culture, the medium was collected, and the amount of TGF-β1 was measured using a TGF-β1 measurement kit (manufactured by r & d systems). Further, the amount of DNA in the petri dish was measured and used as an index of the number of cells. The amount of DNA was measured by a fluorescence measurement method using Hoechst 33258 (manufactured by Hoechst). Note that no cytotoxicity was observed at the above experimental concentrations. When the amount of TGF-β1 per DNA in the extract-free group (control) was taken as 100, the value of the winged bean extract-added group was defined as the TGF-β1 production promotion rate (%). The results are shown in Table 2.

  As apparent from Table 2, the winged bean extract was found to have an excellent TGF-β1 production promoting effect.

4). Collagen gel contractility evaluation test (Evaluation of the effect of human skin fibroblasts on type I collagen gel contractility)
After a fibroblast (5 × 10 ⁴ cell / ml) suspension collagen solution (type I collagen manufactured by Koken Co., Ltd.) was prepared on ice, the collagen was gelled at 37 ° C. Thereafter, a test substance (DMSO or purified water as a control) and 0.25% FBS-containing DMEM medium were added, the gel was peeled from the petri dish wall surface, and collagen contraction was performed. After 1, 2, 3 and 6 days, the diameter of the collagen gel was measured from three directions and the average value was determined. The diameter before shrinkage was taken as 0% shrinkage, and the shrinkage after adding the test substance was determined (each group n = 3). The results are shown in Table 3.

  As is apparent from Table 3, the winged bean extract was found to have an excellent collagen gel contraction promoting effect.

5). Evaluation test of integrin production promoting ability in dermis fibroblasts A winged bean extract was allowed to act on human dermal fibroblasts, 24 hours later, the cells were detached with trypsin / EDTA, neutralized with a serum (FBS) -containing medium, The cells were recovered by washing with PBS containing 01% FCS and 0.02% NaN ₃ . Anti-human integrin α2 as the primary antibody, [alpha] 2 [beta] 1, .beta.1 at 100-fold dilution, respectively, using a FITC-labeled anti-mouse IgG ₁ in 100 dilution as a secondary antibody. The blank used mouse IgG1 as the primary antibody. The amount of integrin on the cell surface was measured using a flow cytometer. The results are shown in Table 4.

6). Evaluation test of integrin production promoting ability in epidermal cells After the action of winged bean extract on HaCaT cells, the cells were detached with trypsin / EDTA 24 hours later, neutralized with FCS, and then the cells were 0.01% FCS, 0.02% NaN _3. The cells were collected by washing with PBS containing. Anti-human integrin α2 as the primary antibody, .alpha.3, [alpha] 2 [beta] 1, .beta.1 at 100-fold dilution, respectively, using a FITC-labeled anti-mouse IgG ₁ in 100 dilution as a secondary antibody. The blank used mouse IgG1 as the primary antibody. The amount of integrin on the cell surface was measured using a flow cytometer. The results are shown in Table 5.

  As described above, it was revealed that winged bean extract enhances integrin production in both fibroblasts and epidermal cells.

  Next, the formulation example of the skin external preparation containing the cell growth promoter of this invention is shown.

Formulation Example 1 Cream (Compounding ingredients) (mass%)
(1) Stearic acid 2.0
(2) Stearyl alcohol 7.0
(3) Hydrogenated lanolin 2.0
(4) Squalane 5.0
(5) 2-Octyldodecyl alcohol 6.0
(6) Polyoxyethylene (25 mol)
Cetyl alcohol ether 3.0
(7) Glycerin monostearate ester 2.0
(8) Propylene glycol 5.0
(9) Cell growth promoter (winged bean 50% ethanol extract) (dry conversion)
0.05
(10) Sodium bisulfite 0.03
(11) Ethylparaben 0.3
(12) Perfume appropriate amount (13) Purified water Residue (Production method)
(8) was added to (13) and dissolved, and heated to 70 ° C. (water phase). On the other hand, (1) to (7) and (9) to (12) were mixed, heated and melted, and kept at 70 ° C. (oil phase). Subsequently, the oil phase was added to the aqueous phase, preliminarily emulsified, and uniformly emulsified with a homomixer, and then cooled to 30 ° C. with good stirring.

Formulation Example 2 Cream (Compounding ingredients) (mass%)
(1) Solid paraffin 5.0
(2) Beeswax 10.0
(3) Vaseline 15.0
(4) Glycerin monostearate ester 2.0
(5) Polyoxyethylene (20 mol)
Sorbitan monolaurate 3.0
(6) Soap powder 0.1
(7) Borax 0.2
(8) TGF-β production promoter (winged bean 70% 1,3-butylene glycol extract) (dry conversion) 0.05
(9) Sodium bisulfite 0.03
(10) Ethylparaben 0.3
(11) Perfume appropriate amount (12) Purified water Residue (Production method)
(6) to (7) were added to (12), dissolved by heating and kept at 70 ° C. (aqueous phase). On the other hand, (1) to (5) and (8) to (11) were mixed, heated and melted, and kept at 70 ° C. (oil phase). Next, the oil phase was gradually added to the aqueous phase while stirring to carry out the reaction. After completion of the reaction, the mixture was uniformly emulsified with a homomixer and then cooled to 30 ° C. with good stirring.

Formulation Example 3 Emulsion (Compounding ingredients) (mass%)
(1) Stearic acid 2.5
(2) Cetyl alcohol 1.5
(3) Vaseline 5.0
(4) Liquid paraffin 10.0
(5) Polyoxyethylene (10 mol)
Monooleate 3.0
(6) Polyethylene glycol 1500 3.0
(7) Triethanolamine 1.0
(8) Carboxyvinyl polymer 0.05
(“Carbopol 941”; BFGoodrich Chemical Company)
(9) Collagen gel shrinkage promoter (winged bean water extract) (dry conversion)
0.0001
(10) Sodium bisulfite 0.01
(11) Ethylparaben 0.01
(12) Perfume appropriate amount (13) Purified water Residue (Production method)
(8) was dissolved in a small amount of (13) (A phase). (6) to (7) were added to the remaining (13), dissolved by heating and kept at 70 ° C. (aqueous phase). On the other hand, (1) to (5) and (9) to (12) were mixed, heated and melted, and kept at 70 ° C. (oil phase). The oil phase was added to the aqueous phase for preliminary emulsification, and the A phase was further added and uniformly emulsified with a homomixer. After emulsification, the mixture was cooled to 30 ° C. with good stirring.

Formulation Example 4 Emulsion (Compounding ingredient) (mass%)
(1) Microcrystalline wax 1.0
(2) Beeswax 2.0
(3) Lanolin 20.0
(4) Liquid paraffin 10.0
(5) Squalane 5.0
(6) Sorbitan sesquioleate ester 4.0
(7) Polyoxyethylene (20 mol)
Sorbitan monooleate 1.0
(8) Propylene glycol 7.0
(9) Integrin production promoter (winged bean 50% 1,3-butylene glycol extract) (dry conversion) 10.0
(10) Sodium bisulfite 0.01
(11) Ethylparaben 0.3
(12) Perfume appropriate amount (13) Purified water Residue (Production method)
(8) was added to (13) and heated to 70 ° C. (aqueous phase). On the other hand, (1) to (7) and (9) to (12) were mixed, melted by heating and kept at 70 ° C. (oil phase). The aqueous phase was gradually added while stirring the oil phase, and the mixture was uniformly emulsified with a homomixer. After emulsification, the mixture was cooled to 30 ° C. with good stirring.

Formulation Example 5 Jelly (Compounding ingredients) (mass%)
(1) 95% ethyl alcohol 10.0
(2) Dipropylene glycol 15.0
(3) Polyoxyethylene (50 mol)
Oleyl alcohol ether 2.0
(4) Carboxyvinyl polymer 1.0
(“Carbopol 941”; BFGoodrich Chemical Company)
(5) Caustic soda 0.15
(6) L-arginine 0.1
(7) Cell growth promoter (winged bean 90% 1,3-butylene glycol extract) (dry conversion) 7.0
(8) 2-hydroxy-4-methoxy
Sodium benzophenone sulfonate 0.05
(9) Ethylenediaminetetraacetate
Trisodium dihydrate 0.05
(10) Methylparaben 0.2
(11) Perfume appropriate amount (12) Purified water Residue (Production method)
(4) was uniformly dissolved in (12) (aqueous phase). On the other hand, (7) and (3) were dissolved in (1) and added to the aqueous phase. Next, (2) and (8) to (11) were added thereto, and then neutralized and thickened with (5) and (6).

Formulation Example 6 Beauty Liquid (Compounding ingredients) (mass%)
(Phase A)
(1) 95% ethyl alcohol 10.0
(2) Polyoxyethylene (20 mol) octyldodecanol 1.0
(3) Pantothenyl ethyl ether 0.1
(4) TGF-β production promoter (winged bean 90% ethanol extract) (dry conversion)
1.5
(5) Methylparaben 0.15
(Phase B)
(6) Potassium hydroxide 0.1
(Phase C)
(7) Glycerin 5.0
(8) Dipropylene glycol 10.0
(9) Sodium bisulfite 0.03
(10) Carboxyvinyl polymer 0.2
(“Carbopol 941”; BFGoodrich Chemical Company)
(11) Purified water residue (production method)
The A phase and the C phase were uniformly dissolved, and the A phase was added to the C phase and solubilized. Then phase B was added and filled.

Formulation Example 7 Pack (mixing component) (mass%)
(Phase A)
(1) Dipropylene glycol 5.0
(2) Polyoxyethylene (60 mol) hydrogenated castor oil 5.0
(Phase B)
(3) Collagen gel contraction promoter (winged bean 90% ethanol extract) (dry conversion)
0.01
(4) Olive oil 5.0
(5) Tocopherol acetate 0.2
(6) Ethylparaben 0.2
(7) Fragrance 0.2
(Phase C)
(8) Sodium bisulfite 0.03
(9) Polyvinyl alcohol (degree of saponification 90, degree of polymerization 2000) 13.0
(10) Ethanol 7.0
(11) Purified water residue (production method)
A phase, B phase, and C phase were uniformly dissolved, and B phase was added to A phase to solubilize. C phase was then added and filled.

Formulation Example 8 Solid Foundation (Composition Component) (mass%)
(1) Talc 43.1
(2) Kaolin 15.0
(3) Sericite 10.0
(4) Zinc flower 7.0
(5) Titanium dioxide 3.8
(6) Yellow iron oxide 2.9
(7) Black iron oxide 0.2
(8) Squalane 8.0
(9) Isostearic acid 4.0
(10) POE sorbitan monooleate 3.0
(11) Isocetyl octanoate 2.0
(12) Integrin production promoter (winged bean 50% ethanol extract) (dry conversion)
1.0
(13) Preservative appropriate amount (14) Fragrance appropriate amount (production method)
(1)-(7) powder components are sufficiently mixed with a blender, (8)-(11) oily components, (12), (13), (14) are added to this and kneaded well. Filled and molded.

Formulation Example 9 Emulsification Foundation (Cream Type)
(Compounding ingredients) (mass%)
(Powder part)
(1) Titanium dioxide 10.3
(2) Celiteite 5.4
(3) Kaolin 3.0
(4) Yellow iron oxide 0.8
(5) Bengala 0.3
(6) Black iron oxide 0.2
(Oil phase)
(7) Decamethylcyclopentasiloxane 11.5
(8) Liquid paraffin 4.5
(9) Polyoxyethylene-modified dimethylpolysiloxane 4.0
(Water phase)
(10) Purified water 50.0
(11) 1,3-butylene glycol 4.5
(12) Cell growth promoter (winged bean 30% 1,3-butylene glycol extract) (dry conversion) 0.001
(13) Preservative appropriate amount (14) Fragrance appropriate amount (production method)
After the aqueous phase was heated and stirred, the powder part sufficiently mixed and ground was added and homomixed. Furthermore, after adding the heat-mixed oil phase and carrying out a homomixer process, the fragrance | flavor was added while stirring and it cooled to room temperature.

Formulation Example 10 Cookie (Compounding ingredients) (mass%)
Soft flour 45.0
Butter 17.5
Granulated sugar 20.0
TGF-β production promoter (winged bean extract) (dried product) 4.0
Carrot extract appropriate amount Egg 12.5
Lemon flavor 1.0
(Manufacturing method)
While stirring butter, granulated sugar was gradually added, and then egg, winged bean extract, carrot extract and flavor were added and further stirred. After sufficiently stirring, a weakly shaken flour was added, stirred at low speed, and agglomerated and laid in a refrigerator. Then, it shape | molded and baked at 170 degreeC for 15 minutes, and was set as the cookie.

Formulation Example 11 Ointment (Compounding ingredients) (mass%)
Collagen gel contraction promoter (winged bean extract) 2.0
Artemisia extract 1.0
Stearyl alcohol 18.0
Owl 20.0
Polyoxyethylene (20) monooleate 0.25
Glycerin monostearate 0.3
Vaseline 40.0
Purified water residue (production method)
The winged bean extract is added to purified water and dissolved, and kept at 70 ° C. (aqueous phase). The remaining components are mixed and dissolved at 70 ° C. (oil phase). An oil phase was added to the aqueous phase, and the mixture was uniformly emulsified with a homomixer and then cooled to obtain an ointment.

Claims (4)

  A cell growth promoter comprising an extract of one or more plants selected from plants belonging to the genus Psophocarpus.   A TGF-β production promoter characterized by comprising an extract of one or more plants selected from plants belonging to the genus Psophocarpus.   A collagen gel contraction promoter comprising an extract of one or more plants selected from plants belonging to the genus Psophocarpus).   An integrin production promoter characterized by comprising an extract of one or more plants selected from plants belonging to the genus Psophocarpus).