JP2008088075A - Profilaggrin/filaggrin production promoter, epidermal keratinocyte proliferation promoter, skin care preparation for normalizing epidermis/horny cell layer, profilaggrin/filaggrin production-promoting method and epidermal keratinocyte proliferation-promoting method - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a new profilaggrin/filaggrin production promoter, an epidermal keratinocyte proliferation promoter, a skin care preparation excellent in epidermis/horny cell layer normalization effects, a profilaggrin/filaggrin production-promoting method, and an epidermal keratinocyte proliferation-promoting method. <P>SOLUTION: The profilaggrin/filaggrin production promoter contains a Chloranthus japonicus extract as an active ingredient. The epidermal keratinocyte proliferation promoter also contains the Chloranthus japonicus extract as an active ingredient. The skin care preparation for normalizing epidermis/horny layer, contains the above agents. The profilaggrin/filaggrin production-promoting method or the epidermal keratinocyte proliferation method comprises supplying the Chloranthus japonicus extract. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a profilagrin and / or filaggrin production promoter and an epidermal keratinocyte proliferation promoter useful as an active ingredient of a skin external preparation such as cosmetics. The present invention also relates to a skin external preparation for normalizing the epidermis / horny layer, a method for promoting profilaggrin and / or filaggrin production, and a method for promoting epidermal keratinocyte proliferation.

  Conventionally, for the purpose of imparting prescribed medicinal effects to skin external preparations such as emulsions, creams, lotions, packs, cleaning agents, dispersions, ointments, solutions, aerosols, patches, poultices, liniments, etc. Various medicinal ingredients are added. In recent years, various causes of skin troubles have been elucidated, and along with this, consumer needs for external preparations for skin, particularly cosmetics, are being subdivided. For example, it is known that filaggrin is involved in the internal construction of stratum corneum cells and is essential for moisture retention within the skin. In addition, it is known that a decrease in metabolic function of epidermal keratinocytes due to aging or the like contributes to a delay in wound healing of the epidermis. Therefore, it is desired to provide an agent capable of normalizing the stratum corneum or epidermis by promoting the production of filaggrin through the expression of profilagrin or promoting the proliferation of epidermal keratinocytes.

On the other hand, since the safety to the skin is also important as an active ingredient to be blended in the external preparation for skin, various plant extracts that are agents derived from natural products have been proposed as active ingredients for external preparations for skin. Yes. For example, a moisturizing agent composed of a human lizard extract has been proposed (Patent Document 1).
Japanese Patent No. 3696862

An object of the present invention is to provide a novel profilagrin / filaggrin production promoter and epidermal keratinocyte proliferation promoter that are highly safe for the skin and are useful as an active ingredient of an external preparation for skin.
Another object of the present invention is to provide a skin external preparation for normalizing the stratum corneum or normalizing the epidermis, which is excellent in the effect of normalizing the stratum corneum or epidermis.
Another object of the present invention is to provide a novel method for promoting the production of profilaggrin / filaggrin and a novel method for promoting the proliferation of epidermal keratinocytes.

In order to solve the above-mentioned problems, the present invention provides a profilagrin / filaggrin production promoter and an epidermal keratinocyte proliferation promoter, which comprises human lysizuka extract as an active ingredient.
From another point of view, according to the present invention, a skin external preparation for normalizing the epidermis containing a human lizard extract as an active ingredient; a skin external preparation for normalizing the stratum corneum containing a human lizard extract as an active ingredient; Provided is a method of promoting profilaggrin / filaggrin production by providing; and a method of promoting proliferation of epidermal keratinocytes by supplying human lysizuka extract.

  ADVANTAGE OF THE INVENTION According to this invention, the novel agent and novel method which can accelerate | stimulate the production of a profilagrin / filaggrin, and the novel agent and novel method which can accelerate | stimulate proliferation of an epidermal keratinocyte can be provided. Moreover, according to this invention, the skin external preparation excellent in the epidermis normalization or stratum corneum normalization effect can be provided.

Hereinafter, the present invention will be described in detail. In the present specification, “to” means a range including numerical values before and after.
The profilaggrin / filaggrin production promoter and epidermal keratinocyte growth promoter of the present invention contain an extract of the Coriander family Chloranthus japonicus (scientific name: Chloranthus japonicus) as an active ingredient. There is no restriction | limiting about an extraction site | part, and the extract of any parts, such as a root, stem, a leaf, an inflorescence, a fruit, and a seed of a human lizardfish, may be sufficient as the human lizard extract used for this invention. In addition, the profilagrin / filaggrin production promoter and epidermal keratinocyte proliferation promoter of the present invention may be mixed with extracts obtained from two or more places of human lysizuka, or different solvents from two or more parts. Two or more kinds of extracts extracted by the above may be mixed and used.

  The human lizard extract is obtained by extracting one or two or more places such as roots, stems, leaves, inflorescences, fruits, seeds, etc. of human lizards with an appropriate solvent and distilling off the solvent. These portions may be subjected to an appropriate treatment such as drying, shredding, pressing, or fermentation, followed by an extraction treatment. Extraction can be carried out by immersing human lizard in a solvent at a low temperature or under warming for a predetermined time. The extraction solvent is not particularly limited, but water or lower monohydric alcohols such as methyl alcohol and ethyl alcohol; liquid polyhydric alcohols such as glycerin, propylene glycol and 1,3-butylene glycol; ketones such as acetone; ethyl ether and the like 1 type or 2 types or more of organic solvents, such as these ethers; or esters, such as ethyl acetate; and the mixed solvent of these and water can be used.

  The human squirrel extract may be used as it is in a skin external preparation, or may be used after being left to stand for an appropriate period and aged. If necessary, it can be used after further purification treatment such as filtration, deodorization and decolorization with an ion exchange resin or the like within a range not affecting the effect. Further, only a fraction having a high activity can be used by using a separation means such as liquid chromatography.

  Examples of preferred methods for preparing the human lizard extract include human lizards, alcoholic solvents such as hydrous or non-hydrated ethyl alcohol, polyhydric alcohol solvents such as hydrous or non-hydrated 1,3-butylene glycol, or water. The mixture is filtered at room temperature or warmed for 1 to 5 days, filtered, and the filtrate obtained is left to mature for about one week, and the filtrate is filtered again.

Examples of the components in the extract include flavonoid glycosides, phenols, amino acids, triterpenes and the like. The higher the content of the alcohol solvent or polyhydric alcohol solvent in the extraction solvent, the higher the triterpene content.
In the human lysizuka extract used for the profilagrin / filaggrin production promoter of the present invention, the extraction solvent is preferably a mixed solvent of water and a monohydric alcohol, more preferably a mixed solvent of water and ethyl alcohol. More preferably, it is a mixed solvent of water and ethyl alcohol in which the mixing ratio of ethyl alcohol is 50% by volume (hereinafter referred to as “vol%”) or more.
Moreover, it is preferable that the extract of the human lizards extract used for the epidermal keratinocyte growth promoter of the present invention is only purified water.

  The human lizard extract can be used as an extract, dried by spray drying or the like, or in a powder form, or a dry solid or powder mixed with an appropriate solvent according to the intended use. That is, it may be used as an active ingredient of a profilagrin / filaggrin production promoter and an epidermal keratinocyte proliferation promoter in any form such as liquid, paste or gel.

Here, the relationship between the promotion of production of profilagrin / filaggrin and the promotion of proliferation of keratinocytes and the effect of normalizing the stratum corneum and normalizing the epidermis will be described.
An amino acid that is a main component of a natural moisturizing factor (NMF) is produced by degrading filaggrin derived from keratohyalin granules in the stratum corneum. This filaggrin is expressed as profilagrin in epidermal keratinocytes present in the granular layer immediately below the stratum corneum. After that, it is phosphorylated immediately, accumulated in keratohyalin granules, dephosphorylated and hydrolyzed, then decomposed into filaggrin, transferred to the stratum corneum, increasing the efficiency of keratin filament aggregation, and the internal construction of stratum corneum cells It has been reported to be involved. Furthermore, it has been reported that this filaggrin is very important and indispensable for moisture retention of the skin, and that the synthetic ability of filaggrin is reduced by conditions such as drying, and the amount of amino acids in the stratum corneum is reduced. Therefore, in epidermal keratinocytes, it is expected to increase the amount of amino acids in the stratum corneum by promoting the synthesis of filaggrin through promoting the expression of profilagrin.
In addition, aging reduces the metabolic function of epidermal keratinocytes, slows the turnover rate of the skin, causes skin aging and rough skin, and retains stratum corneum cells that fall off the stratum corneum surface. It is known that melanin excreted from melanocytes to epidermal keratinocytes is not smoothly discharged, causing pigmentation and skin dullness, and slowing wound healing of the epidermis. .
That is, by promoting the production of profilagrin / filaggrin, the water environment in the stratum corneum is adjusted, or by promoting the proliferation of epidermal keratinocytes, the metabolism of the epidermis is promoted, and the excellent effect of normalizing the stratum corneum and the epidermis Normalization effect can be expected.

  The profilagrin / filaggrin production promoter and epidermal keratinocyte proliferation promoter of the present invention can be used for various applications. Since the safety to the skin is good, it is preferably blended into the external preparation for skin. In addition, the profilaggrin / filaggrin production promoter and epidermal keratinocyte proliferation promoter of the present invention can also be blended in foods (including beverages). Although it does not restrict | limit especially as said foodstuff, Specifically, a salmon, chewing gum, a drink etc. are mentioned, for example.

Next, the skin external preparation of the present invention will be described in detail.
The skin external preparation of the present invention is a skin external preparation for normalization of the epidermis / horny layer, which contains, as an active ingredient, a human squirrel extract, which is the profilagrin / filaggrin production promoter or epidermal keratinocyte proliferation promoter of the present invention. is there. Although there is no restriction | limiting in particular about the compounding quantity of the said agent, The said human squirrel extract is 0.00005 to 1.0005 as solid content with respect to the total mass of the skin external preparation which is a final form. It is preferably mass% (hereinafter, simply referred to as “%”), more preferably 0.0005 to 1.5%. Within this range, the components can be stably blended, and a high profilaggrin / filaggrin production promoting action and / or epidermal keratinocyte proliferation promoting action is achieved, and the horny layer and / or epidermis based thereon A normalizing action can be exerted. In addition, when the extract is used, the concentration of the extract is not limited as long as the content of the human squirrel extract as a solute is within the above range.

  The form of the external preparation for skin of the present invention is not particularly limited, and examples thereof include emulsions, creams, lotions, cosmetics, packs, cleaning agents, makeup cosmetics, dispersions, ointments, solutions, aerosols, patches, and cataplasms. Any form of cosmetic, such as a liniment, may be an external medicine.

  In addition to the radical scavenger, the skin external preparation of the present invention includes various components that are usually used in cosmetics, quasi-drugs, external medicines, ie, water, alcohols, oils, surfactants, Viscosity, powder, chelating agent, pH adjuster, UV absorber, extracts from animals and plants / microorganisms, various medicinal agents such as moisturizers, whitening agents, anti-inflammatory agents, cell activators, fragrances, etc. As long as the effect is not impaired, it can be added as appropriate.

  The present invention also relates to a method for promoting the production of profilaggrin / filaggrin and a method for promoting proliferation of epidermal keratinocytes by supplying a human squirrel extract. For example, it may be applied to the surface of the skin and utilized to normalize the stratum corneum and / or epidermis. The stratum corneum and / or epidermis can be normalized and used to improve the appearance of the skin, i.e. for cosmetic purposes.

  Hereinafter, the present invention will be described more specifically with reference to examples. However, the scope of the present invention is not limited to the following examples.

[Example 1: Manufacture of 80 vol% hydrous ethyl alcohol extract of human lizards]
150 g of 80% hydrous ethyl alcohol (the ratio of ethyl alcohol is 80 vol%) is added to 10 g of human squirrel grass, extracted at room temperature or heated for one day, filtered, and the solvent in the obtained filtrate is filtered. Distilled to dryness, an 80 vol% water-containing ethyl alcohol extract (yield 0.8 g) of human lizards as a solid content was obtained.

[Example 2: Example of production of human lizardka 50 vol% hydrous ethyl alcohol extract]
150 g of 50 vol% hydrous ethyl alcohol (the ratio of ethyl alcohol is 50 vol%) is added to 10 g of human squirrel grass, extracted at room temperature or heated for one day, filtered, and the solvent in the obtained filtrate is filtered. Distilled to dryness to obtain a 50 vol% water-containing ethyl alcohol extract (yield: 0.75 g) of human lizards as a solid content.

[Example 3: Production example of purified water extract of Hitorizuka]
After adding 150 mL of purified water to 10 g of the whole plant of human squirrel and extracting at room temperature or warming for one day, it is filtered, and the solvent in the obtained filtrate is evaporated to dryness. A certain human lizards purified water extract (yield 0.73 g) was obtained.

[Example 4: Evaluation of profilaggrin / filaggrin production promoting action]
Human normal neonatal skin epidermal keratinocytes (NHEK) were cultured in an 80 cm ² flask in medium for normal human neonatal epidermal keratinocytes (KGM) at 37 ° C. under 5% CO ₂ , and cells were collected by a conventional method. . The obtained cells were adjusted to 1.5 × 10 ⁵ cells / mL in the same medium, seeded in 2 mL aliquots on 6-well collagen-coated plates, and cultured at 37 ° C. under 5% CO ₂ for 3 days. After culturing, the sample was replaced with 2 mL of KGM containing or not containing the test sample dissolved in 0.5% DMSO (control) and cultured at 37 ° C. under 5% CO ₂ for 5 days. After completion of the culture, total protein was prepared by a conventional method.

The sample adjusted to 10 μg / lane was developed by SDS-PAGE and transferred to a PVDF membrane. After blocking with PBS (-) containing 5% skim milk, anti-human filaggrin monoclonal antibody (Harbor Bio-Products), biotin-labeled anti-mouse Ig (Whole Ab) (Amersham Biosciences), streptavidin-peroxidase complex (CALBIOCHEM) ) Was diluted 1000-fold with PBS (-) containing 0.1% Tween 20, 0.3% skim milk, and reacted sequentially, and profilagrin and luminescence were detected by luminescence of ECL Western blotting detection reagents and analysis system (Amersham Biosciences). Filaggrin was detected. The detected band was quantitatively measured with KODAK 1D Image Analysis Software EDAS290 Version 3.5.
As a result, the profilaggrin / filaggrin production promoting action of the test sample was evaluated using the net intensity of the profilagrin and filaggrin in 10 μg of the protein prepared from the cells cultured with and without the test sample added. .
The calculation method of profilaggrin / filaggrin production promoting action is as follows.
Profilaggrin / filaggrin production promotion rate (%) = A / B × 100
A: Net intensity at the time of test sample addition
B: Net intensity when no test sample is added (control)
In addition, as each test sample, the aqueous solution (concentration is 2 μg / mL) of the human lizard extract.

[Example 5: Evaluation of epidermal keratinocyte proliferation promoting effect]
Human normal neonatal skin epidermal keratinocytes (NHEK) were cultured using a growth medium for long-term culture of human normal neonatal epidermal keratinocytes (EpoLife-KG2), and then cells were collected by trypsin treatment. The collected cells were diluted with EpoLife-KG2 to a concentration of 1.5 × 10 ⁴ cells / mL, then seeded at 100 μL per well in a 96-well plate, and cultured overnight. After completion of the culture, 100 μL of a test sample dissolved in EpoLife-KG2 was added to each well and cultured for 3 days. Epidermal keratinocyte proliferation was measured using the MTT assay. After completion of the culture, the medium was removed, and 100 μL of MTT dissolved in PBS (−) at a final concentration of 0.4 mg / mL was added to each well. After culturing for 2 hours, blue formazan produced in the cells was extracted with 100 μL of 2-propanol. After extraction, the absorbance at a wavelength of 570 nm was measured. At the same time, the absorbance at a wavelength of 650 nm was measured as turbidity, and the difference between the two was used as the amount of blue formazan produced. In addition, it shows that epidermal keratinocyte proliferation promotion effect is so high that there is much production amount of blue formazan.
The calculation method of the epidermal keratinocyte proliferation action is as follows.
Epidermal keratinocyte proliferation promotion rate (%) = St / Ct × 100
St: Absorbance in the cells to which the test sample was added Ct: Absorbance in the cells to which the test sample was not added In addition, an aqueous solution (concentration is described in the following table) of the human lysizuka extract was used as each test sample.

[Example 6: lotion]
A lotion was prepared by the composition shown in Table 3 and the following production method, and the effect of improving the rough skin (normalized stratum corneum and normalized skin) of the human lizard extract was examined. The results are also shown in Table 3.

(Composition and results)

(Manufacturing method)
A. Components (1) to (8) are mixed and dissolved.
B. Components (9) to (12) are mixed and dissolved.
C. A and B were mixed and uniformed to obtain a skin lotion.

  About the lotion of the said this invention product and the comparative product, the rough skin improvement effect was determined with the method shown below. The test subjects were 22-38 year-old women with symptoms such as xeroderma, atopic dermatitis, and rough skin. Each group applied 20 test samples to the facial cheek twice a day (morning and evening). Then, a continuous test for 4 weeks was conducted. The skin surface condition (texture, desquamation) before and after the continuous test was observed with a microscope, and was evaluated in three stages according to the improvement status: “improvement”, “slight improvement”, and “no change”.

<Evaluation criteria>
A: The number of subjects evaluated as “improvement” is 15 or more. ○: The number of subjects evaluated as “improvement” is 10-14. Δ: The number of subjects evaluated as “improvement” is 5-9. 1 to 4 subjects evaluated as “Improved”

  From the results shown in Table 3, the lotions of the products 1 to 3 of the present invention formulated with 80% ethyl alcohol extract, 50% ethyl alcohol extract, and purified water extract of human lizards should be applied to the skin. Thus, it is clear that the effect of improving rough skin is exhibited.

Hereinafter, examples of blending into cosmetics will be shown, but not limited to these prescriptions.
[Example 7: Cleansing cream]
(Ingredient) (%)
(1) Stearic acid 2.0
(2) Stearyl alcohol 3.0
(3) Lipophilic glyceryl monostearate 2.0
(4) Beeswax 1.5
(5) Vaseline 6.0
(6) Liquid paraffin 40.0
(7) Dimethylpolysiloxane (100CS) 0.5
(8) Sorbitan sesquioleate 1.0
(9) Preservative appropriate amount (10) Triethanolamine 1.0
(11) Propylene glycol 10.0
(12) Polyethylene glycol 20000 0.5
(13) Carboxyvinyl polymer 0.05
(14) Purified water Residual amount (15) Human liquor 50 vol% hydrous ethyl alcohol extract * 1 0.2
(16) Hop extract * 2 0.5
(17) Soybean extract * 3 0.5
(18) Fragrance Appropriate amount * 1: What was obtained by dissolving the extract produced in Example 2 in 50 vol% hydrous 1,3-butylene glycol to a content of 1% * 2: Maruzen Pharmaceutical Co., Ltd. * 3: Maruzen Pharmaceutical Co., Ltd. Made (Manufacturing method)
A. Ingredients (1) to (9) are dissolved by heating and maintained at 70 ° C.
B. Ingredients (10) to (14) are dissolved by heating and maintained at 70 ° C.
C. A is added to B and emulsified.
D. After cooling C, components (15) to (18) were added and mixed to obtain a cleansing cream.
The obtained cleansing cream was a mild cleansing cream that spreads lightly and has good makeup stains, and has a healthy stratum corneum and epidermis and maintains healthy skin.

[Example 8: Face wash]
(Ingredient) (%)
(1) Lauric acid 5.0
(2) Myristic acid 18.5
(3) Stearic acid 6.0
(4) Glycerin 12.0
(5) Polyethylene glycol 1500 5.0
(6) Potassium hydroxide 6.5
(7) Purified water remaining amount (8) Palm oil fatty acid diethanolamide 5.0
(9) Palm oil fatty acid methyl taurine sodium 1.8
(10) Polyoxyethylene (7.5E.O.) lauryl ether 2.0
(11) Ethylene glycol distearate 1.0
(12) Hydroxypropyl methylcellulose 1% aqueous solution 5.0
(13) Hitori-Sizuka 50vol% hydrous ethyl alcohol extract * 1 0.5
(14) Soybean extract * 2 0.5
(15) Mukuroji extract * 3 0.5
(16) Fragrance Appropriate amount * 1: What was obtained by dissolving the extract produced in Example 2 in 50 vol% hydrous 1,3-butylene glycol to a content of 1% * 2: Maruzen Pharmaceutical Co., Ltd. * 3: Maruzen Pharmaceutical Co., Ltd. Made (Manufacturing method)
A. Components (1) to (7) are dissolved by heating.
B. Components (8) to (11) are dissolved by heating.
C. Add B to A and mix.
D. After cooling C, components (12) to (16) are added and mixed to obtain a face wash.
The resulting cleansing cream and facial cleanser had a fine and rich foaming and a refreshing feel, and was a facial cleanser that maintained a healthy skin by making the stratum corneum and epidermis healthy. .

[Example 9: Lotion 1]
(Ingredient) (%)
(1) Citric acid 0.05
(2) Sodium citrate 0.2
(3) Sodium pyrrolidonecarboxylate (50%) solution * 1 0.5
(4) Glycerin 3.0
(5) 1,3-butylene glycol 8.0
(6) L-ascorbic acid 2-glucoside * 2 2.0
(7) Sodium hydroxide 0.25
(8) Hitorishizuka 80vol% hydrous ethyl alcohol extract * 3 1.0
(9) Glucosyl trehalose * 4 1.0
(10) Tencha extract * 5 1.0
(11) Purified water remaining amount (12) Ethyl alcohol 10.0
(13) Perfume Appropriate amount (14) Preservative Appropriate amount (15) Polyoxyethylene monooleate (20E.O.)
Sorbitan 0.5
* 1: Ajinomoto Co., Inc. * 2: Hayashibara Biochemical Laboratories Co., Ltd. * 3: The extract produced in Example 1 dissolved in 50 vol% hydrous 1,3-butylene glycol so as to have a content of 1% * 4 : Hayashibara Biochemical Laboratories * 5: Maruzen Pharmaceutical Co., Ltd. (production method)
A. Components (1) to (11) are mixed and dissolved.
B. Components (12) to (15) are mixed and dissolved.
C. B was added to A and mixed to obtain a skin lotion.
The obtained lotion 1 had a fresh and refreshing feeling of use, and was a lotion that made the stratum corneum and epidermis healthy, kept the skin fresh and smoothed the skin.

[Example 10: Lotion 2]
(Ingredient) (%)
(1) Meadow home oil 0.1
(2) Jojoba oil 0.05
(3) Perfume appropriate amount (4) preservative appropriate amount (5) polyoxyethylene monooleate (20E.O.)
Sorbitan 0.5
(6) Isostearic acid polyoxyethylene hydrogenated castor oil (50 EO) 1.0
(7) Ethyl alcohol 8.0
(8) Glycerin 5.0
(9) 1,3-butylene glycol 5.0
(10) Polyethylene glycol 1500 0.1
(11) Hitorishizuka 80vol% hydrous ethyl alcohol extract * 1 1.0
(12) Dipotassium glycyrrhizinate * 2 0.5
(13) Yeast extract * 3 0.5
(14) Remaining amount of purified water * 1: The extract produced in Example 1 was dissolved in 50 vol% hydrous 1,3-butylene glycol so as to have a content of 1%. * 2: Maruzen Pharmaceutical Co., Ltd. * 3: Maruzen Made by Pharmaceutical Company (Manufacturing method)
A. Components (1) to (7) are mixed and dissolved.
B. Components (8) to (14) are mixed and dissolved.
C. A is added to B and mixed to obtain a skin lotion.
The obtained lotion 2 had a fresh and mellow feeling of use, and was a lotion that made the stratum corneum and epidermis healthy, kept the skin fresh and smoothed the skin.

[Example 11: Lotion 3]
(Ingredient) (%)
(1) Glyceryl tri-2-ethylhexanoate 0.08
(2) Squalane 0.02
(3) Sorbitan sesquioleate 0.05
(4) Polyoxyethylene monooleate (20E.O.)
Sorbitan 0.05
(5) Polyoxyethylene (8E.O.) alkylene (12-15)
Ether phosphoric acid 0.1
(6) Preservative appropriate amount (7) perfume appropriate amount (8) ethyl alcohol 8.0
(9) Dipropylene glycol 8.0
(10) Glycerin 4.0
(11) Hitorishizuka 50 vol% hydrous ethyl alcohol extract * 1 0.5
(12) Carrot extract * 2 0.5
(13) Yokuinin extract * 3 0.5
(14) Remaining amount of purified water * 1: The extract prepared in Example 2 dissolved in 50 vol% water-containing 1,3-butylene glycol so as to have a content of 1% * 2: Maruzen Pharmaceutical Co., Ltd. * 3: Maruzen Made by Pharmaceutical Company (Manufacturing method)
A. Components (1) to (8) are mixed and dissolved.
B. Components (9) to (14) are mixed and dissolved.
C. A is added to B and emulsified to obtain a skin lotion.
The obtained lotion 3 had a clean and light feeling of use, and was a lotion that made the stratum corneum and epidermis healthy, kept the skin fresh and smoothed the skin.

[Example 12: Latex]
(Ingredient) (%)
(1) Stearic acid 1.0
(2) Cetanol 0.5
(3) Lipophilic glyceryl monostearate 0.5
(4) Liquid paraffin 2.0
(5) Squalane 3.0
(6) Jojoba oil 3.0
(7) Cetyl palmitate 0.2
(8) Preservative appropriate amount (9) Sorbitan monostearate 0.3
(10) Polyoxyethylene monooleate (20E.O.)
Sorbitan 0.5
(11) Tocopherol * 1 0.1
(12) Triethanolamine 0.5
(13) 1,3-butylene glycol 15.0
(14) Glycerin 3.0
(15) Polyethylene glycol 6000 0.5
(16) Purified water remaining amount (17) 1% solution of carboxyvinyl polymer 8.0
(18) Human squirrel 50 vol% hydrous ethyl alcohol extract * 2 0.5
(19) Neubara extract * 3 0.5
(20) Peonies extract * 4 0.5
(21) Fragrance Appropriate amount * 1: Eisai * 2: The extract produced in Example 2 dissolved in 50 vol% hydrous 1,3-butylene glycol so as to have a content of 1% * 3: Maruzen Pharmaceutical Co., Ltd. * 4: Made by Maruzen Pharmaceutical Co., Ltd. (Production method)
A. Components (1) to (11) are dissolved by heating and maintained at 70 ° C.
B. Components (12) to (16) are dissolved by heating and maintained at 70 ° C.
C. B is added to A to emulsify, and component (17) is further added and mixed.
D. C is cooled, and components (18) to (21) are added and mixed to obtain an emulsion.
The obtained emulsion had a smooth and mellow feeling of use, and was an emulsion that made the stratum corneum and epidermis into a healthy state, imparted a moisturizing feeling and an appropriate emollient feeling to the skin, and made the skin soft.

[Example 13: Cream]
(Ingredient) (%)
(1) Stearic acid 2.5
(2) Cetanol 2.5
(3) Lipophilic glyceryl monostearate 2.0
(4) Vaseline 2.0
(5) Dipentaerythritol fatty acid ester * 1 2.0
(6) Isotridecyl myristate 5.0
(7) Liquid paraffin 8.0
(8) Squalane 5.0
(9) Beeswax 1.0
(10) Cetyl palmitate 2.0
(11) Sorbitan sesquioleate 0.5
(12) Polyoxyethylene monooleate (20E.O.)
Sorbitan 1.5
(13) Preservative appropriate amount (14) Triethanolamine 1.2
(15) 1,3-butylene glycol 8.0
(17) Glycerin 2.0
(17) Polyethylene glycol 20000 0.5
(18) Purified water Remaining amount (19) Carboxyvinyl polymer 1% aqueous solution 10.0
(20) Panthenol * 2 0.5
(21) Human squirrel 80 vol% hydrous ethyl alcohol extract * 3 1.0
(22) Nicotinamide * 4 0.5
(23) Rosemary extract * 5 0.5
(24) Warmoko extract * 6 0.5
(25) Perfume appropriate amount * 1: Cosmol 168AR (Nisshin Oillio Group)
* 2: Wako Pure Chemical Industries, Ltd. * 3: The extract produced in Example 1 was dissolved in 50 vol% hydrous 1,3-butylene glycol so as to have a content of 1%. * 4: Wako Pure Chemical Industries, Ltd. * 5: Made by Maruzen Pharmaceutical * 6: Made by Maruzen Pharmaceutical (Production method)
A. Ingredients (1) to (13) are dissolved by heating and maintained at 70 ° C.
B. Ingredients (14) to (18) are dissolved by heating and maintained at 70 ° C.
C. B is added to A to emulsify, and component (19) is further added and mixed.
D. C is cooled, components (20) to (25) are added and mixed to obtain a cream.
The obtained cream had a smooth and rich feeling of use, was a cream that made the stratum corneum and epidermis in a healthy state, imparted a high emollient feeling to the skin, and softened the skin.

[Example 14: Cosmetic liquid]
(Ingredient) (%)
(1) Glyceryl tri-2-ethylhexanoate 0.1
(2) Meadow Home Oil 0.05
(3) Jojoba oil 0.05
(4) Retinol palmitate * 1 0.2
(5) Tocopherol acetate * 2 0.1
(6) Preservative appropriate amount (7) perfume appropriate amount (8) polyoxyethylene monooleate (20E.O.)
Sorbitan 0.5
(9) Polyoxyethylene hydrogenated isostearate castor oil (50 EO) 1.5
(10) Ethyl alcohol 5.0
(11) Glycerin 4.0
(12) Dipropylene glycol 8.0
(13) 1,3-butylene glycol 8.0
(14) Sodium lactate 0.5
(15) Sodium pyrrolidonecarboxylate (50%) solution * 3 0.5
(16) Hydroxyethyl cellulose 0.08
(17) Sodium alginate 0.05
(18) Human lizards 80% hydrous ethyl alcohol extract * 4 0.2
(19) Licorice extract * 5 0.5
(20) Gentian extract * 6 0.5
(21) Remaining amount of purified water * 1: Roche * 2: Eisai * 3: Ajinomoto * 4: 50 vol% water containing 1,3 so that the extract produced in Example 1 has a content of 1% -Dissolved in butylene glycol * 5: manufactured by Maruzen Pharmaceutical Co., Ltd. * 6: manufactured by Maruzen Pharmaceutical Co., Ltd. (production method)
A. Components (1) to (9) are mixed and dissolved.
B. Components (10) to (21) are mixed and dissolved.
C. A is added to B and mixed to obtain a serum.
The obtained beauty liquid has a mellow and mild feeling of use, is a beauty liquid that makes the stratum corneum and epidermis healthy, gives the skin a high moisturizing and emollient feeling, and makes the skin fresh and soft. It was.

[Example 15: Pack (Peel-off type)]
(Ingredient) (%)
(1) Polyvinyl alcohol 12.0
(2) Methylcellulose 0.1
(3) Glycerin 3.0
(4) 1,3-butylene glycol 5.0
(5) Purified water remaining amount (6) perfume appropriate amount (7) preservative appropriate amount (8) glyceryl tri-2-ethylhexanoate 0.1
(9) Polyoxyethylene monooleate (20E.O.)
Sorbitan 1.0
(10) Ethyl alcohol 13.0
(11) Human squirrel purified water extract * 1 0.5
(12) Oat extract * 2 0.5
(13) Ononis extract * 3 0.5
* 1: The extract produced in Example 3 was dissolved in 50 vol% water-containing 1,3-butylene glycol so as to have a content of 1%. * 2: Maruzen Pharmaceutical Co., Ltd. * 3: Maruzen Pharmaceutical Co., Ltd. (manufacturing method)
A. Components (1) to (5) are dissolved by heating.
B. Components (6) to (10) are mixed and dissolved.
C. After cooling A, B and components (11) to (13) are added and mixed to obtain a pack.
The resulting pack has a moderate cool feeling and high tension, makes the stratum corneum and epidermis in a healthy state, gives the skin after packing a proper moisturizing feeling and elasticity, and makes the skin flexible. It was a pack to do.

[Example 16: Massage cream]
(Ingredient) (%)
(1) Stearic acid 2.0
(2) Stearyl alcohol 2.5
(3) Lipophilic glyceryl monostearate 2.0
(4) Sorbitan sesquioleate 1.0
(5) Cetyl palmitate 1.0
(6) Dipentaerythritol fatty acid ester * 1 4.0
(7) Vaseline 20.0
(8) Liquid paraffin 28.0
(9) Dimethylpolysiloxane (100CS) 0.5
(10) Sodium hydroxide 0.1
(11) Dipropylene glycol 7.0
(12) Carboxyvinyl polymer 0.1
(13) Purified water Remaining amount (14) Human lyska 50 vol% hydrous ethyl alcohol extract * 2 0.5
(15) Sodium hyaluronate 1% solution * 3 5.0
(16) Mannitol * 4 0.2
(17) Perfume appropriate amount * 1: Cosmol 168AR (Nisshin Oillio Group)
* 2: The extract produced in Example 2 was dissolved in 50 vol% hydrous 1,3-butylene glycol so that the content was 1%. * 3: Kibun Food Chemifa Co., Ltd. * 4: Wako Pure Chemical Industries, Ltd. )
A. Ingredients (1) to (9) are dissolved by heating and maintained at 70 ° C.
B. Components (10) to (13) are dissolved by heating and maintained at 70 ° C.
C. A is added to B and emulsified.
D. After cooling C, components (14) to (17) were added and mixed to obtain a massage cream.
The resulting massage cream has a rich and smooth feeling of use, has a high massage effect, makes the stratum corneum and epidermis in a healthy state, gives the skin a moisturizing and sticking feel, and makes the skin smooth It was a cream.

[Example 17: Liquid foundation]
(Ingredient) (%)
(1) Stearic acid 2.0
(2) Cetanol 0.5
(3) Behenyl alcohol 1.0
(4) Petrolatum 2.5
(5) Liquid paraffin 5.0
(6) Self-emulsifying glyceryl monostearate 1.0
(7) Preservative appropriate amount (8) Titanium oxide 6.0
(9) Color pigment 4.0
(10) Mica 2.0
(11) Talc 4.0
(12) Carboxymethylcellulose 0.2
(13) Bentonite 0.4
(14) Purified water Residual amount (15) Hitorizuzka 80vol% hydrous ethyl alcohol extract * 1 0.5
(16) Ganoderma extract * 2 0.5
(17) Chamomile extract * 3 0.5
(18) Fragrance Appropriate amount * 1: The extract produced in Example 1 dissolved in 50 vol% hydrous 1,3-butylene glycol so as to have a content of 1% * 2: Maruzen Pharmaceutical Co., Ltd. * 3: Maruzen Pharmaceutical Co., Ltd. Made (Manufacturing method)
A. Components (1) to (7) are dissolved by heating.
B. Ingredients (8) to (11) are added to A, mixed uniformly, and kept at 70 ° C.
C. Ingredients (12) to (14) are dissolved by heating and maintained at 70 ° C.
D. B is added to C and emulsified.
E. After cooling D, components (15) to (18) were added and mixed to obtain a liquid foundation.
The obtained liquid foundation was a liquid foundation that had a light and expansive feeling of use, made the stratum corneum and epidermis in a healthy state, and had a uniform and beautiful finish.

  The profilagrin and / or filaggrin production promoter and epidermal keratinocyte growth promoter of the present invention can be used as an active ingredient for external preparations for skin, foodstuffs and the like. In particular, it is useful for external preparations for skin such as cosmetics.

Claims (6)

Profilaggrin and / or filaggrin production promoter comprising human lizard extract as an active ingredient. An epidermal keratinocyte proliferation promoter comprising human lysizuka extract as an active ingredient. A skin external preparation for normalizing the epidermis, which contains a human lizard extract as an active ingredient. A skin external preparation for normalizing the stratum corneum, which contains a human lizard extract as an active ingredient. A method of promoting the production of profilagrin and / or filaggrin by supplying a human lysizuka extract (except for a method for human treatment). A method of promoting proliferation of epidermal keratinocytes by supplying a human lysizuka extract (however, excluding human treatment methods).