JP2009298728A - Cell activator and external preparation for skin for preventing senescence - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a new cell activator utilizing a component originated from a natural product and having good safety, and to provide an external preparation for the skin for anti-ageing by utilizing the cell activator. <P>SOLUTION: The cell activator contains an extract of seed coat of a plant belonging to the genus Juglans of Juglandaceae as an active ingredient. The external preparation for the skin for anti-ageing contains the cell activator as an active ingredient. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to a novel cell activator and an anti-aging skin external preparation using the same as an active ingredient.

Conventionally, seeds of walnuts belonging to the genus Walnut are used for food. In addition to edible products, walnut oil, walnut extract, walnut nut milk, and the like extracted from seeds have been proposed for use as active ingredients in skin external preparations such as skin cosmetics (for example, Patent Document 1). ~ 6). On the other hand, walnut seed coats and extracts thereof are proposed in Patent Document 7 as hepatoprotectants. Patent Document 8 proposes the use of walnut seed coats and their extracts as skin-beautifying components. In the examples, the melanin production rate is calculated for a sample to confirm the whitening effect.
JP 2003-212714 A JP 2003-292952 A JP 2004-75573 A JP 2004-331634 A JP 2005-53842 A Japanese Patent No. 3911164 JP 2008-74807 A JP 2008-81408 A

  An object of the present invention is to provide a novel cell activator with good safety using a component derived from a natural product, and an anti-aging skin external preparation using the same.

As a result of intensive studies by the present inventor, the seed coat extract of the walnut family walnut plant has a high activation effect on fibroblasts, and even if it is added to the extract skin external preparation, its cell activation effect Has been found to be effective, and based on this finding, the present invention has been completed.
That is, in order to solve the above problems, the present invention provides a cell activator comprising an extract of a seed coat of a walnut family walnut plant as an active ingredient, and an anti-aging skin external preparation containing the cell activator as an active ingredient. provide.
One embodiment of the present invention is a fibroblast activator comprising, as an active ingredient, an extract of a seed coat of a walnut family walnut plant.
Moreover, it is preferable that an example of the said extract is an extract containing 5 mass% or more of polyphenols.
In addition, the extract may be an extract of the seed coat of the walnut genus Walnut genus Juglans mandshurica Maxim. Var.
From another point of view, according to the present invention, a method for normalizing and / or activating fibroblasts in the dermis by applying a seed coat extract of a walnut plant belonging to the walnut family to the skin (however, human Are excluded).

  ADVANTAGE OF THE INVENTION According to this invention, the novel cell activator with favorable safety | security and the skin external preparation for anti-aging which contains the said cell activator as an active ingredient can be provided.

Hereinafter, the present invention will be described in detail. In the present specification, “to” means a range including numerical values before and after.
The present invention relates to a cell activator comprising, as an active ingredient, an extract of a seed coat of a plant belonging to the genus Walnut family (hereinafter sometimes referred to as “walnut seed coat extract”). In the present invention, the seed coat of any plant belonging to the genus Walnut may be used. Examples thereof include the Japanese walnut Juglans mandshurica Maxim. Var. Sachalinensis, the Japanese walnut Juglans mandshurica Maxim var. Cordiformis, and the Japanese walnut Juglans. Regia L. var. Orientis. The seeds of the genus Walnut plant are called nuts and are used for food. In the present invention, the seed coat peeled from the seed is used. Sites other than the seed coat may be mixed, but in order to extract an active ingredient having a cell activation effect at a high concentration, it is preferable to perform the extraction process using only the seed coat as a raw material.

  The walnut seed coat extract can be prepared by a general method. Although it does not specifically limit as an extraction solvent, For example, Water; Lower monohydric alcohols, such as methyl alcohol and ethyl alcohol; Liquid polyhydric alcohols, such as glycerol, propylene glycol, and 1, 3- butylene glycol; Ketones, such as acetone; Ethyl One or more of ethers such as ether; esters such as ethyl acetate; and the like can be used. It is preferable to use a mixed solution of water and a lower monohydric alcohol (for example, ethyl alcohol). The extraction is carried out in the solvent for a predetermined time (for example, 1 to 0 ° C. in the case where the seed coat of walnut is at room temperature or under heating (for example, a temperature near the boiling point of the solvent. For about 48 hours). It is preferable to stir the system during the extraction process. In addition, pre-treatment such as drying, shredding, pressing or fermentation can be performed on the walnut seed coat before extraction.

  The walnut seed coat extract can be used directly as a cell activator after preparation. Further, if desired, it can be used as a cell activator after being left to mature for an appropriate period. If necessary, it can be used after being subjected to a purification treatment such as deodorization and decolorization by filtration or ion exchange resin within a range that does not affect the effect of the present invention. Further, a fraction having high activity can be taken out and used by using a separation means such as liquid chromatography.

  Examples of a preferable method for preparing a walnut seed coat extract include water, lower monohydric alcohol such as methanol, ethanol, etc .; glycerin, propylene glycol; It can be obtained by extraction after heating in a hydrophilic polar solvent such as polyhydric alcohol such as 1,3-butylene glycol; or a mixed solvent of two or more of these or immersing at room temperature. However, the extraction method is not limited to this.

  The walnut seed coat extract may be in any form such as liquid, paste or gel. The liquid extract containing the extraction solvent can also be used after it is solidified by drying under reduced pressure or freeze drying. It can also be dried by spray drying or the like and used as a powder.

  As a result of investigation by the present inventors, it was found that the walnut seed coat extract has a high cell activation effect on fibroblasts. Fibroblasts are cells that exist in the dermis and produce collagen, elastin, hyaluronic acid, etc., and are known to contribute to forming a dermal structure by using collagen as a fiber bundle. If these fibroblasts are damaged or decreased by aging or exposure to ultraviolet rays, regular fibers cannot be produced, and skin aging such as wrinkles and sagging occurs. Therefore, this walnut seed coat extract having an activation action on fibroblasts is useful as an active ingredient of a skin external preparation, particularly a skin external preparation for preventing aging, and is excellent in anti-aging when applied to the skin. It exerts its action and can effectively improve skin wrinkles and sagging caused by aging, exposure to ultraviolet rays, and the like.

  Further, as a result of the study by the present inventors, the walnut seed coat extract contains various components. Among them, the polyphenols contribute to the cell activation action, and the ratio of the polyphenols in the solid content of the extract. It has been found that the cell activation action is particularly high when the content is 5% by mass or more. From the viewpoint of cell activation, the content of polyphenols in the solid content of the extract is more preferably 10% by mass or more, and further preferably 30% by mass or more.

  The present invention also relates to an anti-aging skin external preparation containing, as an active ingredient, a walnut seed coat extract that is the cell activator of the present invention. The external preparation for skin of the present invention exhibits an excellent anti-aging effect due to the cell activation action of the walnut seed coat extract. The content of the walnut seed coat extract in the external preparation for skin of the present invention is preferably 0.00001 to 2% by mass (hereinafter simply referred to as “%”), more preferably 0.0001 to 1 as the solid content. %. If it exists in this range, the said extract can be mix | blended stably and the high anti-aging effect can be exhibited.

  The external preparation for skin of the present invention can be prepared by formulating the cell activator by blending it with various forms of bases according to a conventional method. Furthermore, by preparing the above-mentioned cell activator in combination with one or more other medicinal agents, the cell activation effect is further enhanced, or a skin external preparation that exhibits other medicinal effects together with the cell activation effect is prepared. Can do. Examples of other medicinal agents include, but are not limited to, whitening agents, UV protection agents, antibacterial agents, anti-inflammatory agents, other cell activators, active oxygen scavengers, and moisturizers. Absent.

  Examples of whitening agents include ascorbic acid or derivatives thereof, arbutin, ellagic acid, linoleic acid, vitamin E and derivatives thereof, glycyrrhizic acid and derivatives thereof, tranexamic acid, placenta extract, chamomile extract, licorice extract, age extract , Ogon extract, seaweed extract, cucumber extract, caquette extract, gokahi extract, rice bran extract, wheat germ extract, saicin extract, hawthorn extract, sun penz extract, shirayuri extract, peony extract Sempukuka extract, soybean extract, tea extract, molasses extract, sandalwood extract, grape extract, hop extract, micaika extract, mokka extract, yukinoshita extract and the like.

  Examples of the UV protection agent include paramethoxycinnamate-2-ethylhexyl, 2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5 sodium sulfate, 4-t-butyl-4′- Examples thereof include methoxydibenzoylmethane, 2-phenyl-benzimidazole-5-sulfuric acid, titanium oxide, and zinc oxide.

  Examples of the antibacterial agent include benzoic acid, sodium benzoate, p-hydroxybenzoate ester, benzalkonium chloride, phenoxyethanol, isopropylmethylphenol, and the like.

  Anti-inflammatory agents include, for example, sulfur and its derivatives, glycyrrhizic acid and its derivatives, glycyrrhetinic acid and its derivatives, artea extract, ashitaba extract, arnica extract, ginseng extract, nettle extract, buckwheat extract, hypericum Examples include extracts, chamomile extracts, goldfish extract, watercress extract, comfrey extract, salvia extract, sicon extract, perilla extract, birch extract, gentian extract and the like.

  Examples of other cell activators include caffeine, chicken crown extract, shell extract, shell extract, royal jelly, silk protein and its degradation product or derivatives thereof, lactoferrin or its degradation product, chondroitin sulfate, hyaluronic acid Mucopolysaccharides or their salts, collagen, yeast extract, lactic acid bacteria extract, bifidobacteria extract, fermentation metabolic extract, ginkgo extract, barley extract, assembly extract, diatom extract, carrot extract, Rosemary extract, glycolic acid, citric acid, lactic acid, malic acid, tartaric acid, succinic acid and the like are included.

  The active oxygen scavenger has an action such as suppression of lipid peroxide production. For example, superoxide dismutase, mannitol, quercetin, catechin and derivatives thereof, rutin and derivatives thereof, button pi extract, yashajitsu extract, melissa extract , Lanahan fruit extract, retinol and its derivatives, vitamin A such as carotenoid, thiamine and its derivative, riboflavin and its derivative, pyridoxine and its derivative, vitamin B such as nicotinic acid and its derivative, tocopherol and its derivative, etc. Vitamin E, dibutylhydroxytoluene, butylhydroxyanisole, etc. are mentioned.

  Examples of the humectant include proteins such as elastin and keratin or derivatives thereof, hydrolysates and salts thereof, amino acids such as glycine, serine, aspartic acid, glutamic acid, arginine and theanine and derivatives thereof, sorbitol, erythritol, Trehalose, inositol, glucose, sucrose and derivatives thereof, dextrin and derivatives thereof, saccharides such as honey, D-panthenol and derivatives thereof, urea, phospholipid, ceramide, auren extract, ginger extract, diautum extract, sensyu extract Product, mallow extract, gypsophila extract, dokudami extract, hamamelis extract, bodaige extract, maronier extract, quince extract and the like.

  Moreover, arbitrary components other than the cell activator of this invention can be mix | blended with the skin external preparation of this invention. Examples of such components include, but are not limited to, physiologically active substances such as amino acids, lipids, sugars, hormones, enzymes, and nucleic acids. In addition, components that are usually used in the production of cosmetics, quasi-drugs, skin external preparations and the like, for example, water (purified water, hot spring water, deep water, etc.), oil agents, Surfactant, metal soap, gelling agent, powder, alcohol, water-soluble polymer, film-forming agent, resin, inclusion compound, fragrance, deodorant, salt, pH adjuster, refreshing agent, plant / animal -Extracts derived from microorganisms, blood circulation promoters, astringents, antiseborrheic agents, chelating agents, keratolytic agents, enzymes, hormones, other vitamins and the like can be used as necessary.

  The external preparation for skin of the present invention has various forms such as powders such as powder and powder foundation; solids such as soap and lipstick; emulsions such as cream, emulsion and cream foundation; It is preferable that it is a cosmetic composition. However, it is not limited to these.

EXAMPLES Hereinafter, the present invention will be described more specifically with reference to examples. However, the scope of the present invention is not limited to the following examples.
[Example 1: Preparation of sample]
-Walnut seed coat extract As the walnut seed coat extract used in this test, walnut polyphenol-PC30 (walnut polyphenol manufactured by Oriza Oil Chemical Co., Ltd.), which is a water-containing ethanol extract from the seed coat of the walnut family (Juglans regia L.), was used. 30% or more) was used.
-Preparation of walnut seed oil (for comparative example) Walnut seeds (seed coat removed) were squeezed to prepare walnut seed oil. This was used as a sample for a comparative example.

[Example 2: Cell activation test example]
Human neonatal fibroblasts NB1RGB were used.
First, an appropriate amount of a medium was added to a 96-well plate, and fibroblasts NB1RGB were seeded there, and allowed to stand in an atmosphere at a temperature of 37 ° C. and a carbon dioxide concentration of 5 vol%. On the next day, the sample preparation solution was added and mixed so that the final concentrations of the walnut seed coat extract and walnut seed oil of Example 1 were 0 (control), 1, 10, and 100 μg / mL (medium), respectively. On day 4 of the culture, the medium was changed, and the sample preparation solution was added again. The next day, the medium is removed, the cells are washed after washing with phosphate buffer, and the number of fibroblasts NB1RGB grown in each sample preparation solution is determined by the following MTT assay method. Cell activation was evaluated using a sample to which no sample was added as a control.

(Evaluation method by MTT assay)
The medium grown by adding each sample preparation solution is replaced with a fresh medium, and after adding MTT assay reagent and culturing, the absorbance at a wavelength of 490 nm is measured to determine the amount of formazan, and no sample preparation solution is added. Cell activation was evaluated as 100%.
The results are shown in the table below.

From the results in Table 1, it can be understood that the walnut seed coat extract has a high cell activation ability with respect to human neonatal fibroblast NB1RGB. Therefore, by applying the cell activator of the present invention containing walnut seed coat extract as an active ingredient to the skin, it exhibits an extremely excellent anti-aging effect, such as skin wrinkles, sagging caused by aging, UV exposure, etc. Can be effectively improved.
On the other hand, as shown in Table 2, the walnut seed oil obtained by squeezing the seed excluding the seed coat had no cell activation effect on human neonatal fibroblast NB1RGB.

[Example 3: Preparation example of lotion]
A lotion 1 having the following composition was prepared by the following method.
(Manufacturing method)
A. The following components (1) to (7) are mixed and dissolved.
B. The following components (8) to (11) are mixed and dissolved.
C. B was added to A and mixed to obtain a skin lotion.
(Ingredient)%
(1) Citric acid 0.05
(2) Sodium citrate 0.2
(3) Sodium pyrrolidonecarboxylate (50%) solution 0.5
(4) Walnut seed coat extract * 1 0.05
(5) Glycerin 3.0
(6) 1,3-butylene glycol 8.0
(7) Purified water remaining amount (8) Ethyl alcohol 10.0
(9) Perfume Appropriate amount (10) Preservative Appropriate amount (11) Polyoxyethylene monooleate (20E.O.)
Sorbitan 0.5
* 1 Walnut polyphenol-PC30 manufactured by Oriza Oil Chemical Co., Ltd.

  The prepared lotion 1 was a lotion that had a fresh feeling of use, was excellent in moisture of the skin, adjusted the texture, and was able to make the skin beautiful and elastic.

[Example 4: Preparation of emulsion]
An emulsion having the following composition was prepared by the following method.
(Manufacturing method)
A. The following components (1) to (10) are dissolved by heating and maintained at 70 ° C.
B. The following components (11) to (15) are dissolved by heating and maintained at 70 ° C.
C. B is added to A to emulsify, and the following component (16) is further added and mixed.
D. C is cooled, the following components (17) and (18) are added and mixed to obtain an emulsion.
(Ingredient)%
(1) Stearic acid 1.0
(2) Cetanol 0.5
(3) Lipophilic glyceryl monostearate 0.5
(4) Liquid paraffin 2.0
(5) Squalane 3.0
(6) Jojoba oil 3.0
(7) Cetyl palmitate 0.2
(8) Preservative appropriate amount (9) Sorbitan monostearate 0.3
(10) Polyoxyethylene monooleate (20E.O.)
Sorbitan 0.5
(11) Triethanolamine 0.5
(12) 1,3-butylene glycol 15.0
(13) Glycerin 3.0
(14) Polyethylene glycol 6000 0.5
(15) Purified water Remaining amount (16) Carboxyvinyl polymer 1% solution 8.0
(17) Walnut seed coat extract * 1 0.2
(18) Fragrance Appropriate amount * 1 Walnut polyphenol-PC30 manufactured by Oriza Oil Chemical Co., Ltd.

  The prepared emulsion had a fresh and smooth feeling of use, was excellent in moisture and emollient, had a smooth texture, and was able to give beautiful skin with elasticity.

[Example 5: Preparation example of cream]
The following compositional claims were prepared in the following manner.
(Manufacturing method)
A. The following components (1) to (13) are dissolved by heating and maintained at 70 ° C.
B. The following components (14) to (18) are dissolved by heating and maintained at 70 ° C.
C. B is added to A to emulsify, and the following component (19) is further added and mixed.
D. C is cooled, and the following components (20) and (21) are added and mixed to obtain an emulsion.
(Ingredient)%
(1) Stearic acid 2.5
(2) Cetanol 2.5
(3) Lipophilic glyceryl monostearate 2.0
(4) Vaseline 2.0
(5) Dipentaerythritol fatty acid ester * 1 2.0
(6) Isotridecyl myristate 5.0
(7) Liquid paraffin 8.0
(8) Squalane 5.0
(9) Beeswax 1.0
(10) Cetyl palmitate 2.0
(11) Sorbitan sesquioleate 0.5
(12) Polyoxyethylene monooleate (20E.O.)
Sorbitan 1.5
(13) Preservative appropriate amount (14) Triethanolamine 1.2
(15) 1,3-butylene glycol 8.0
(16) Glycerin 2.0
(17) Polyethylene glycol 20000 0.5
(18) Purified water remaining amount (19) 1% aqueous solution of carboxyvinyl polymer 5.0
(20) Walnut seed coat extract * 2 1.0
(21) Fragrance appropriate amount * 1: Cosmol 168AR (Nisshin Oillio Group)
* 2 Walnut polyphenol-PC30 manufactured by Oriza Oil Chemical Co., Ltd.

  The prepared cream was a cream that had a smooth and smooth feeling of use, excellent skin emollient, smoothed texture, and, after all, made beautiful skin with elasticity.

[Example 6: Preparation example of pack]
A pack having the following composition was prepared by the following method.
(Manufacturing method)
A. The following components (1) to (5) are dissolved by heating.
B. The following components (6) to (10) are mixed and dissolved.
C. After cooling A, B and the following (11) were added and mixed to obtain a pack.
(Ingredient)%
(1) Polyvinyl alcohol 12.0
(2) Methylcellulose 0.1
(3) Glycerin 3.0
(4) 1,3-butylene glycol 5.0
(5) Purified water remaining amount (6) perfume appropriate amount (7) preservative appropriate amount (8) glyceryl tri-2-ethylhexanoate 0.1
(9) Polyoxyethylene monooleate (20E.O.)
Sorbitan 1.0
(10) Ethyl alcohol 13.0
(11) Walnut seed coat extract * 1 0.1
* 1 Walnut polyphenol-PC30 manufactured by Oriza Oil Chemical Co., Ltd.

  The prepared pack was a pack that stretched well, imparted moderate tension to the skin, did not stick to the skin after peeling off the pack, and was highly moist and sticky.

[Example 7: Preparation example of cream foundation]
A cream foundation having the following composition was prepared by the following method.
(Manufacturing method)
A: The following components (8) to (13) are mixed.
B: The following components (14) to (19) are mixed.
C: The following components (1) to (7) are added to A and mixed and dispersed.
D: Add B to C and mix.
E: The following components (20) and (21) were added to D to obtain a cream foundation.
(Ingredient)%
(1) Sericite 8.0
(2) Titanium oxide 10.0
(3) Bengala 1.0
(4) Yellow iron oxide 2.2
(5) Mica titanium 2.0
(6) Black iron oxide 0.1
(7) Organic metamorphic bentonite * 1 0.5
(8) Long chain alkyl-containing polyoxyalkylene modified organopolysiloxane * 2 2.0
(9) Polyoxyalkylene-modified organopolysiloxane * 3 2.0
(10) Decamethylcyclopentasiloxane 12.0
(11) Octamethylcyclotetrasiloxane 10.0
(12) Trimethylsiloxysilicic acid silicone solution * 4 3.0
(13) Glyceryl tri-2-ethylhexanoate 5.0
(14) 1,3-butylene glycol 5.0
(15) Diglycerin 2.0
(16) Glycerin 2.0
(17) Xanthan gum 0.2
(18) Sodium chloride 0.2
(19) Purified water remaining amount (20) Walnut seed coat extract * 5 0.1
(21) Perfume appropriate amount * 1: Benton 38 (manufactured by NL Endowry)
* 2: Avil EM-90 (Gold Schmidt)
* 3: KF-6017 (manufactured by Shin-Etsu Chemical Co., Ltd.)
* 4: KF-7312J (manufactured by Shin-Etsu Chemical Co., Ltd.)
* 5: Walnut polyphenol-PC30 manufactured by Oriza Oil Chemical Co., Ltd.

  The prepared cream foundation has a smooth, non-sticky feel, excellent adhesion to the skin, a beautiful finish, and gives an emollient feel to the skin to make it beautiful and elastic. It was a cream foundation.

  ADVANTAGE OF THE INVENTION According to this invention, the novel cell activator with favorable safety | security using the component derived from a natural product, and the skin external preparation for anti-aging using the same can be provided.

Claims (4)

A cell activator comprising as an active ingredient an extract of a seed coat of a walnut family walnut plant. The cell activator according to claim 1, wherein the extract is an extract containing 5 mass% or more of polyphenols. The cell activator according to claim 1 or 2, wherein the extract is an extract of a seed coat of the sea urchin Juglans mandshurica Maxim. Var. . The skin external preparation for anti-aging which contains the cell activator of any one of Claims 1-3 as an active ingredient.