JP2009058355A - Capillary unit and capillary electrophoresis apparatus - Google Patents

Abstract

Provided is a capillary electrophoresis device that does not cause an electric shock to a human body when an electrophoresis unit provided with a capillary tube is connected to a measuring device.
A first container having a first electrode immersed in a buffer solution and a first connection terminal for connecting the electrode to the outside; a second electrode immersed in a buffer solution; and A second container having a second connection terminal for connecting the electrode to the outside, and a third container having a capillary tube for performing electrophoresis using the first and second electrodes. In the capillary unit, the third container is arranged to connect the first container and the second container, and the second electrode of the second container and the second connection terminal A capillary unit provided with a winding portion for generating a voltage according to a change in magnetic field.
[Selection] Figure 9

Description

  The present invention relates to a capillary electrophoresis apparatus, and more particularly to a technique for handling a small capillary electrophoresis apparatus.

  Conventionally, a plate-type gel electrophoresis apparatus has been used as a method for analyzing depending on the molecular weight of DNA, protein, etc. However, since measurement takes time, an apparatus capable of measuring at high speed has been demanded. Therefore, a capillary electrophoresis apparatus using a capillary tube filled with a gel or a fluid polymer is used instead of a flat gel.

This capillary electrophoresis apparatus will be described with reference to FIGS. FIG. 12 is a cross-sectional view showing a configuration of an electrophoresis apparatus using a conventional capillary, and FIG. 13 is a cross-sectional view showing a capillary electrophoresis apparatus in which an electrophoresis channel and a chamber are unitized. In FIG. 12, reference numeral 101 denotes an apparatus main body, and a high voltage power source 105 and a current detection unit 106 are provided inside the apparatus. The voltage generated from the high-voltage power source 105 is applied to the electrodes 102a and 102b, and generates an electric field in the capillary tube 104 provided in the cassette 107 via the buffer solutions 108a and 108b in the containers 109a and 109b, respectively. Similarly, in FIG. 13, a high-voltage power supply 205 and a current detection unit 206 are provided inside the apparatus main body 201. A voltage generated from the high-voltage power supply 205 is applied to the electrodes 202a and 202b, and an electric field is generated in the flow path 204 via the buffer solution in the chambers 208a and 208b formed in the electrophoresis unit 207 (for example, Patent Document 1). See).
JP 2006-284185 A

  However, in the above-described conventional configuration, since the migration path formed by the capillary tube is elongated, a high voltage of about several tens of kV must be applied to both ends of the channel. For this reason, when an electrophoresis unit provided with a capillary tube is connected to a measuring apparatus, there is a problem that a human body touches an electrode where a high voltage is generated to cause an electric shock.

  The present invention solves the above-described conventional problems, and an object of the present invention is to provide a capillary electrophoresis apparatus that does not cause an electric shock to a human body when an electrophoresis unit provided with a capillary tube is connected to a measuring apparatus.

  According to the capillary unit and the capillary electrophoresis apparatus of the present invention, since the high voltage is generated inside the electrophoresis unit provided with the capillary tube, when the electrophoresis unit provided with the capillary tube is connected to the measuring device, A safe capillary electrophoresis system that does not cause an electric shock to the human body can be realized.

  To achieve this object, the capillary unit and the capillary electrophoresis apparatus of the present invention have a first electrode having a first electrode immersed in a buffer solution and a first connection terminal for connecting the electrode to the outside. A second container having a container, a second electrode immersed in a buffer solution, and a second connection terminal for connecting the electrode to the outside; and using the first and second electrodes, In a capillary unit comprising a third container having a capillary for carrying out electrophoresis, the third container is disposed so as to connect the first container and the second container, and the second container A winding portion for generating a voltage in response to a magnetic field change is provided between the second electrode and the second connection terminal.

  Furthermore, a capillary unit and a capillary electrophoresis apparatus according to the present invention include a unit holding unit for holding the capillary unit according to claim 1 and a first connection terminal for detecting an electrophoresis current of the capillary unit. A current detection unit connected to the first connection terminal to ground, a variable power supply connected to the second connection terminal of the capillary unit, and the voltage generation unit in the capillary unit A winding for applying a predetermined magnetic field to the winding, a waveform generating circuit for supplying a current to the winding, and an asymmetrical alternating current for outputting an asymmetrical alternating current to the waveform generating circuit so that an electrophoretic current can be detected from the current detection unit. And a control unit.

Hereinafter, embodiments of a capillary unit and a capillary electrophoresis apparatus of the present invention will be described in detail with reference to the drawings.
(Embodiment 1)
The configuration of each block of the capillary unit according to the first embodiment of the present invention will be described with reference to FIGS. FIG. 1 is a perspective view of a capillary unit in the present embodiment, and FIG. 2 is a cross-sectional view thereof. The capillary unit includes buffer solution holding blocks 1 and 2 that contain a buffer solution, a capillary block 3, and a sample injection block 4. The buffer holding blocks 1 and 2 are provided with injection ports 6 and 7 and air vent holes 8 and 9 for injecting the buffer solution on the upper surface. When the buffer solution is injected from the injection ports 6 and 7, Since the air in the liquid holding block escapes from the air vent holes 8 and 9, the liquid holding block can be injected without resistance.

  Electrodes 21a and 21b are provided inside the buffer solution holding blocks 1 and 2 so as to be immersed in the buffer solution. Further, the buffer solution holding block 1 is provided with a gel injection port 10 and an air vent hole 11 for injecting the electrophoresis medium, and a dialysis membrane 24 is provided inside, and a portion 50b for holding the buffer solution and a portion 50c for holding the electrophoresis medium. And a portion 50a containing a voltage generator 20 that generates a voltage by a change in magnetic field.

  The electric field generated by the voltage generator 20 in the buffer solution holding block 1 while the buffer solution injected from the buffer solution inlet 6 is not mixed with the electrophoresis medium by setting the osmotic membrane 24, the electrode 21a and the buffer solution It can be generated in the capillary block 3 via the electrophoresis medium. Connection terminals 22a and 22b provided in the buffer solution holding blocks 1 and 2 are installed to enable electrical connection with the electrophoresis apparatus. The voltage generator 20 is composed of windings and ferrite.

  Next, the sample injection block 4 will be described. In the upper part of the sample injection block 4, a hole 12 for injecting a sample and a hole 13 for extracting air are provided. Further, both the left and right ends are connected by a through hole, and both ends are sandwiched between the capillary block 3 and the buffer solution holding block 2, so that the inside of the sample injection block 4 is between the capillary tube 14 in the capillary block 3 and the capillary tube 16 in the buffer solution block 2. A gap can be formed.

  Next, the capillary block 3 will be described. The capillary block 3 is a portion where the sample injected into the sample injection block 4 actually undergoes electrophoresis, and the capillary 14 is sandwiched and bonded by a resin material such as acrylic. Reference numeral 15 denotes a detection window 15 for optically detecting the concentration of the sample that has been electrophoresed in the capillary tube 14, and at least the capillary tube 14 is not covered with a coating for maintaining strength in the detection window 15 portion. Further, a temperature control hole 25 penetrating perpendicularly to the longitudinal direction of the capillary tube 14 is provided, and a part of the capillary tube 14 is exposed. By configuring in this way, it is possible to send air at a predetermined temperature into the temperature adjusting hole 25 and control the temperature inside the capillary tube 14.

  In addition, as is well known, when it is desired to accurately separate the components contained in the sample by elongating the capillary tube 14 (in order to improve the separation ability), the capillary tube 14 in the temperature control hole 25 is formed in a ring shape. Thus, the temperature-controlled electrophoresis can be realized without changing the structure of both ends, the position of the detection window 15, the position of the temperature adjustment hole 25, and the like.

  By connecting the buffer solution holding blocks 1 and 2 to the sample injection block 4 and the capillary block 3, a capillary unit 301 as shown in FIG. 2 is obtained. The capillaries 14 and 16 are arranged on the same line, and a gap having a constant width is provided in the sample injection block 4 part. The gap is filled with the sample injected from the sample injection port 12. Furthermore, the electrophoresis medium injected into the gel holding unit 50c in the buffer solution holding block 1 is configured to flow into the capillary tube 14 when the pressure of the gel holding unit 50c is increased.

  Next, the configuration of an electrophoresis apparatus for performing electrophoresis by connecting this capillary unit will be described. FIG. 3 is a cross-sectional view showing a portion related to voltage application of the electrophoresis apparatus of the present embodiment. Reference numeral 32 denotes a magnetic field generator that generates a magnetic field using windings and ferrite, 36 is a low-voltage variable power source that generates a low-voltage, and 37 is a current detector that detects current. Reference numeral 33a denotes a connection terminal for electrical connection with the capillary unit, and the connection terminal 33a is connected to the low voltage variable power source 36. The other end of the low-voltage variable power source 36 is installed on the ground, and the potential of the connection terminal 33a is a low voltage that does not affect the human body with respect to the ground, for example, about DC 50V. The connection terminal 33b is also grounded via the current detection unit 37, and the potential of the connection terminal 33b is the same as that of the ground. Reference numeral 34 denotes a detection unit that detects a sample flowing in the capillary unit with light. Although other mechanisms such as a temperature control unit (not shown) are also provided, they are omitted here because they are not directly related to the present invention.

  Next, a voltage application method according to the present invention will be described with reference to FIGS. FIG. 4 is a cross-sectional view showing a state in which the capillary unit according to the present embodiment is attached to the electrophoresis apparatus. The capillary unit is preliminarily filled with the electrophoresis medium in the capillary tube 14 and the gel holding unit 50c, the sample is held in the sample holding unit 23, and the buffer solution holding unit 50b and the buffer solution holding block 2 are included. Is filled with buffer. The connection terminals 22a and 22b of the capillary unit are respectively connected to the connection terminals 33a and 33b of the electrophoresis apparatus, and the detection unit 34 of the electrophoresis apparatus is inserted into the detection window 15 of the capillary unit.

  FIG. 5 is a block diagram thereof. The magnetic field generator 32 of the electrophoresis apparatus and the voltage generator 20 of the capillary unit are arranged in a positional relationship such that the magnetic field generated by the magnetic field generator 32 induces the voltage generator 20 to generate a voltage. In addition, the magnetic field generator 32, the low-voltage variable power source 36, and the current detector 37, each including the winding 41 and the waveform generation circuit 42, are connected to the control unit 44. The control unit 44 controls the magnetic field generation unit 32 and the low voltage variable power source 36 to acquire detection data from the current detection unit 37.

  Next, the voltage for electrophoresis will be described. Since the electrode 21b is electrically connected to the connection terminal 33b and thus has the same potential as that of the ground, the potential of the electrode 21a is generated from the potential supplied from the low-voltage variable power source 36 having one end connected to the ground and the voltage generator 20. It is the sum of the potential. For example, if the voltage value of the low-voltage variable power supply is 50V and the voltage value Vx output from the voltage generator 20 is 800V, the potential difference Ve between the electrode 21a and the electrode 21b is 800 + 50 = 850V.

  The voltage value Vx output from the voltage generation unit 20 will be described. In normal electromagnetic induction, a sine wave generated from a commercial power supply is often used. However, in the sine wave, since Vx generates a voltage similar to the forward and reverse, no electrophoresis occurs. Therefore, in the present embodiment, the control unit 44 controls the magnetic field of the magnetic field generation unit 32 by applying an asymmetric current to the waveform generation circuit 42, and the voltage waveform of the voltage generation unit 20 is as shown in FIG. As shown in a), the voltage is generated so as to be asymmetric between positive and negative.

  FIG. 6A is a graph showing an asymmetric voltage waveform (Vx) of the voltage generator in the present embodiment. As an ideal waveform, only one of positive and negative voltage waveforms is desirable, but in reality, both positive and negative voltages are generated due to the characteristics of voltage generation by the coil. However, it is possible to change the positive / negative voltage ratio as shown in FIGS. 6 (a) and 7 (a). When electrophoresis is performed with a positive voltage, the positive voltage is large and the negative voltage is low. Control. Then, in order to cancel the negative side voltage, a voltage is generated from the low voltage variable power source 36, and the entire waveform of the voltage applied to the electrodes is shifted to positive. For example, assume that a repetitive waveform of 800 V and −50 V is output from the voltage generator 20 as shown in FIG. At this time, when 50V is output from the low voltage variable power source 36, a repetitive waveform of 850V and 0V is obtained between the electrode 21a and the electrode 21b as shown in FIG. 6B. That is, a positive voltage is always applied to enable electrophoresis.

  Similarly, when electrophoresis is performed with a negative voltage, the voltage generator 20 outputs a repeated waveform of −800 V and 50 V shown in FIG. 7A, and outputs −50 V from the low voltage variable power source 36, thereby causing the electrode 21 b to output. As shown in FIG. 7A, the voltage waveform of the viewed electrode 21a becomes a voltage waveform only on the negative side, and electrophoresis can be realized.

  As described above, by using the capillary unit and the electrophoresis apparatus according to the present embodiment, the electrophoresis apparatus body or the surface of the capillary unit does not have a high-pressure portion that affects the human body, so that electrophoresis can be performed safely. In addition, according to the present embodiment, the voltage direction can be switched and a minute current can be detected. Therefore, it is possible to predict the state in the capillary, such as the generation of bubbles, by analyzing the positive and negative charge substances and detecting the current.

(Embodiment 2)
A block diagram of the capillary unit and the electrophoresis apparatus according to the second embodiment of the present invention is shown in FIG. The difference from the first embodiment is that the low-voltage variable power supply is removed. A portion surrounded by a broken line 60 is a capillary unit, and the other portion is an electrophoresis apparatus. Inside the capillary unit, a voltage generating unit including electrodes 61a and 61b, a rectifier circuit 62, a winding 63 and a rectifier circuit 62 is formed. A winding 64 and a waveform generation circuit 65 are provided on the electrophoretic device side, and the magnetic field generation unit is configured by the winding 64 and the waveform generation circuit 65. A control unit 67 is provided to input the current value obtained by the current detection unit 66 and to control the magnetic field of the magnetic field generation unit. Since the electrode 61a is connected to the ground via the voltage generator, and the electrode 61b is connected to the ground via the current detector, the voltage between the electrode 61a and the electrode 61b is the voltage generated by the voltage generator. Value.

  With the above configuration, the control unit 67 applies the asymmetrical alternating current shown in FIGS. 6 and 7 to the waveform generation circuit 65 by the control unit 67, thereby generating a magnetic field change by the magnetic field generation unit. Due to this magnetic field change, the winding 63 of the capillary unit is induced, and either a positive or negative voltage is applied to the electrode 61a by the rectifier circuit 62, thereby enabling electrophoresis. Further, the current during the electrophoresis can also be detected by the current detector 66.

  In the second embodiment, the voltage application direction cannot be automatically switched. However, even if a magnetic field is generated by a sine wave using AC 100 V (50 Hz or 60 Hz) of a general power source, a rectifier circuit 62 is provided. Therefore, it is converted into either a positive or negative voltage to enable electrophoresis.

(Embodiment 3)
FIG. 9 shows a block diagram of the capillary unit and the electrophoresis apparatus according to the third embodiment of the present invention. The difference from the first and second embodiments is that the capillary unit 70 is not provided with a connection contact with the electrophoresis apparatus. A portion surrounded by a broken line 70 is a capillary unit, and the other portion is an electrophoresis apparatus. 71a and 71b are electrodes, 73 is a rectifier circuit, 74 is a winding, and the winding 74 and the rectifier circuit 73 constitute a voltage generating unit.

  The electrophoresis apparatus is provided with a winding 75, a waveform generation circuit 76 for supplying a voltage to both ends of the winding 75, and a current detection unit 77 for detecting a current value flowing through the winding 75, and a control unit The detected current value is sent to 78. The control unit 78 has a function of controlling the waveform of the waveform generation circuit 76 and also includes a calculation unit that calculates the current flowing between the electrodes 71 a and 71 b from the current value obtained by the current detection unit 77.

  With the configuration described above, the winding 74 is induced by the magnetic field generated from the winding 75, and a positive or negative voltage is generated by the rectifier circuit 73, so that a voltage can be applied between the electrode 71a and the electrode 71b. Become.

  In the third embodiment, since the current detection method is indirectly performed from the current value flowing through the winding 63, it is difficult to detect a minute current with high accuracy. There is no need to provide a contact for connection, and maintenance such as cleaning is easy.

(Embodiment 4)
A block diagram of the capillary unit and the electrophoresis apparatus according to the fourth embodiment of the present invention is shown in FIG. The difference from the fourth embodiment is that a plurality of voltage generating units and magnetic field generating units are configured. A portion surrounded by a broken line 80 is a capillary unit, and the other portion is an electrophoresis apparatus. 81a and 81b are electrodes, 82a and 82b are rectifying / smoothing circuits having a voltage waveform rectifying function and a smoothing function, 83a and 83b are windings, and a winding 83a, a rectifying / smoothing circuit 82a, and a winding 83b are rectified and smoothed. The circuit 82b constitutes a voltage generator.

  In the electrophoretic device, a winding 86a and a waveform generation circuit 84a for applying a voltage to the winding 86a, a waveform generation circuit 84b for applying a voltage to the winding 86b and the winding 86b, and a current value flowing through the winding 86a are detected. A current detector 85a and a current detector 85b for detecting a current value flowing through the winding 86b are provided, and the detected current value is sent to the controller 87.

  The control unit 87 has a function of controlling the waveforms of the waveform generation circuits 84a and 84b, and at the same time, a calculation unit that calculates the current flowing between the electrodes 81a and 81b from the current values obtained by the current detection units 85a and 85b. Is also provided.

  With this configuration, a voltage can be applied between the electrodes 81a and 81b as in the third embodiment described above. Furthermore, the rectifying / smoothing circuit 83a has a terminal P1 with respect to the terminal P1, and the rectifying / smoothing circuit 83b has a terminal P1. On the other hand, the voltage between the electrodes 81a and 81b can be switched between positive and negative by configuring so that a voltage with positive P3 is generated. For example, when only the waveform generating circuit 84a is driven, the electrode 81a becomes a positive potential with respect to the electrode 81b via the windings 86a and 83a and the rectifying and smoothing circuit 82a. Similarly, when only the waveform generation circuit 84b is driven, the electrode 81a becomes a negative potential with respect to the electrode 81b via the windings 86b and 83b and the rectifying / smoothing circuit 82b. As described above, according to the present embodiment, it is possible to realize an electrophoresis system capable of applying both positive and negative voltages without providing exposed contacts.

(Embodiment 5)
FIG. 11 shows a block diagram of the capillary unit and the electrophoresis apparatus according to the fifth embodiment of the present invention. Unlike the other embodiments, the carry unit in this embodiment has a polarity switching circuit 94 inside, and does not have a connection contact with the electrophoresis apparatus as in the third and fourth embodiments. A portion surrounded by a broken line 90 is a capillary unit, and the other portion is an electrophoresis apparatus.

  The capillary units 91a and 91b are electrodes, 92 is a rectifier circuit, and 93 is a winding. The winding 93 and the rectifier circuit 92 constitute a voltage generator. 94 is a switch that can be switched by a magnetic field, and is provided to switch the polarity of the voltage generated from the voltage generator.

  The electrophoretic device is provided with a waveform generation circuit 95a for applying a voltage to the winding 96a and a current detection unit 98 for detecting a current value flowing through the winding 96a, and the detected current value is sent to the control unit 97. . In order to switch the switch 94, a winding 96b and a DC power source 99 are provided. The control unit 97 has a function of controlling the waveform of the waveform generation circuit 95, and also includes a calculation unit that calculates the current flowing between the electrodes 91a and 91b from the current value obtained by the current detection unit 98. The DC power source 99 is controlled and the switch 94 is also controlled through the winding 96a.

  With the above configuration, both positive and negative voltages can be applied between the electrode 91a and the electrode 91b as in the fourth embodiment, and at the same time, the current flowing between the electrode 91a and the electrode 91b can be measured.

  In the fourth embodiment, it is necessary to apply an AC voltage for generating a high voltage to the winding 86a and the winding 86b. However, in the fifth embodiment, the winding 96b may be a DC voltage.

 Since the capillary unit according to the present invention can be safely attached to and detached from the electrophoresis apparatus and has a simple configuration, a capillary analysis apparatus that can be carried and used outdoors can be realized. Moreover, since it is small and can perform a safe measurement with a simple operation, a capillary analyzer used in a laboratory or a medical clinical site can be realized.

The perspective view of the capillary unit in Embodiment 1 of this invention Sectional drawing when the capillary unit according to Embodiment 1 of the present invention is coupled Sectional drawing which shows the structure of the part in connection with the voltage application of the electrophoresis apparatus in Embodiment 1 of this invention. Sectional drawing which shows the state which mounted | wore the electrophoresis apparatus with the capillary unit in Embodiment 1 of this invention 1 is a block diagram of a state where a capillary unit according to Embodiment 1 of the present invention is mounted on an electrophoresis apparatus. The wave form diagram which shows the voltage waveform between the electrodes in a capillary unit in Embodiment 1 of this invention The wave form diagram which shows the voltage waveform between the electrodes in a capillary unit in Embodiment 1 of this invention The block diagram which shows the voltage application part of the capillary unit and the electrophoresis apparatus in Embodiment 2 of this invention The block diagram which shows the voltage application part of the capillary unit and the electrophoresis apparatus in Embodiment 3 of this invention FIG. 5 is a block diagram showing a capillary unit and a voltage application unit of an electrophoresis apparatus according to Embodiment 4 of the present invention. FIG. 9 is a block diagram showing a capillary unit and a voltage application unit of an electrophoresis apparatus in a fifth embodiment of the present invention. Sectional drawing which shows the voltage application part of the conventional capillary electrophoresis system Sectional drawing which shows the voltage application part of the conventional chip-type capillary electrophoresis system

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Buffer holding block 2 Buffer holding block 3 Capillary block 4 Sample injection block 5 Electrophoresis medium injection block 6 Inlet 7 Inlet 8 Air vent 9 Air vent 10 Gel inlet 11 Air vent 12 Sample inlet 13 Air vent 14 Capillary DESCRIPTION OF SYMBOLS 15 Detection window 16 Capillary 20 Voltage generation part 21a Electrode 21b Electrode 22a Connection terminal 22b Connection terminal 23 Sample holding part (gap)
24 Dialysis membrane 25 Temperature control hole 31 Electrophoresis device 32 Magnetic field generation unit 33a Connection terminal 33b Connection terminal 34 Detection unit 35 Optical fiber 36 Low voltage variable power supply 37 Current detection unit 38a Electric wire 38b Electric wire 38c Electric wire 38d Electric wire 38e Electric wire 41 Winding 42 Low pressure Variable power supply 43 Current detection unit 44 Control unit 45 Waveform generation circuit 50a Part containing voltage generation part 50b Part holding buffer solution 50c Part holding electrophoresis medium (gel holding part)
60 Capillary Unit 61a Electrode 61b Electrode 62 Rectification Circuit 63 Winding 64 Winding 65 Waveform Generation Circuit 66 Current Detection Unit 67 Control Unit 70 Capillary Unit 71a Electrode 71b Electrode 73 Rectification Circuit 74 Winding 75 Winding 76 Waveform Generation Circuit 77 Current Detection Unit 78 control unit 80 capillary unit 81a electrode 81b electrode 82a rectification smoothing circuit 82b rectification smoothing circuit 83a winding 83b winding 84a waveform generation circuit 84b waveform generation circuit 85a current detection unit 85b current detection unit 86a winding 86b winding 87 control unit 90 Capillary unit 91a Electrode 91b Electrode 92 Rectifier circuit 93 Winding 94 Switch 95 Waveform generating circuit 96a Winding 96b Winding 97 Control unit 98 Current detection Part 99 DC power source 101 Electrophoresis device 102a Electrode 102b Electrode 104 Capillary tube 105 High voltage generation power source 106 Current detection circuit 107 Cartridge 108a Buffer solution 108b Buffer solution 109a Container 109b Container 201 Electrophoresis device 202a Electrode 202b Electrode 204 Microchannel 205 High voltage generation power source 206 Current detection circuit 207 Electrophoresis chip 208a Chamber 208b Chamber 301 Capillary unit P1 terminal P2 terminal P3 terminal

Claims (12)

A first container having a first electrode immersed in a buffer solution and a first connection terminal for connecting the electrode to the outside;
A second container having a second electrode immersed in a buffer solution and a second connection terminal for connecting the electrode to the outside;
In a capillary unit comprising a third container containing a capillary tube for performing electrophoresis using the first and second electrodes,
The third container is arranged to connect the first container and the second container, and a magnetic field change is performed between the second electrode and the second connection terminal of the second container. Capillary unit provided with a winding part that generates a voltage in response to. A first container having a first electrode immersed in a buffer solution and a first connection terminal for connecting the electrode to the outside;
A second container having a second electrode immersed in a buffer solution and a second connection terminal for connecting the electrode to the outside;
In a capillary unit comprising a third container containing a capillary tube for performing electrophoresis using the first and second electrodes,
The third container is disposed so as to connect the first container and the second container, and a voltage is applied between the first connection terminal and the second connection terminal according to a magnetic field change. A capillary unit in which at least one voltage generating unit to be generated is provided in at least one of the first container to the third container. A first container having a first electrode immersed in a buffer solution and a first connection terminal for connecting the electrode to the outside;
A second container having a second electrode immersed in a buffer solution and a second connection terminal for connecting the electrode to the outside;
In a capillary unit comprising a third container containing a capillary tube for performing electrophoresis using the first and second electrodes,
The third container is arranged to connect the first container and the second container, and generates a voltage according to a first magnetic field change, the first connection terminal, and the A polarity switching unit that switches a direction of a current generated in the voltage generation unit according to a second magnetic field change is provided between any one of the first container and the third container. Capillary unit. 4. The capillary unit according to claim 1, wherein the second container further has a gel storage section partitioned by a dialysis membrane between the second electrode and the third container. 5. 4. The capillary unit according to claim 2, wherein the voltage generation unit includes a winding portion made of a winding and a rectification circuit portion that rectifies an AC voltage generated from the winding portion. The capillary unit according to claim 4, wherein the winding portion includes a central magnetic body, and a conductive wire is wound around the magnetic body in a coil shape. 5. The capillary unit according to 4, wherein the voltage generation unit further includes a smoothing circuit unit that smoothes a voltage waveform rectified by the rectification circuit unit. A unit holding part for holding the capillary unit according to claim 1;
A current detector connected to the first connection terminal for detecting an electrophoretic current of the capillary unit and for connecting the first connection terminal to ground;
A variable power source connected to the second connection terminal of the capillary unit;
Windings for applying a predetermined magnetic field to the voltage generation unit in the capillary unit;
A waveform generating circuit for applying a current to the winding;
A capillary electrophoresis apparatus comprising: a control unit for outputting an asymmetrical alternating current to the waveform generation circuit so that an electrophoretic current can be detected from the current detection unit. The capillary electrophoresis apparatus according to claim 8, wherein the variable power source generates a DC voltage of −100V to 100V. A unit holding unit for holding the capillary unit according to claim 1 and connecting the second connection terminal to ground;
A current detector connected to the first connection terminal for detecting an electrophoretic current of the capillary unit and for connecting the first connection terminal to ground;
Windings for applying a predetermined magnetic field to the voltage generation unit in the capillary unit;
A waveform generating circuit for applying a current to the winding;
A capillary electrophoresis apparatus comprising: a control unit for outputting an asymmetrical alternating current to the waveform generation circuit so that an electrophoretic current can be detected from the current detection unit. A unit holder for holding the capillary unit according to claim 2;
Windings for applying a predetermined magnetic field to the voltage generation unit in the capillary unit;
A waveform generating circuit for applying a current to the winding;
A current detector for detecting the current of the waveform generating circuit;
A capillary electrophoresis apparatus comprising: a control unit for outputting an asymmetrical alternating current to the waveform generation circuit so that an electrophoretic current can be detected from the current detection unit. A unit holding part for holding the capillary unit according to claim 3;
A first winding for applying a predetermined magnetic field to the voltage generator in the capillary unit;
A waveform generating circuit for applying a current to the first winding;
A current detector for detecting the current of the waveform generating circuit;
A second winding for applying a predetermined magnetic field to the polarity switching unit in the capillary unit;
A direct current power source for applying a current to the first winding;
An asymmetrical alternating current is output to the waveform generation circuit so that the electrophoretic current can be detected from the current detection unit, and the direction of the current flowing through the second winding is determined according to the polarity of the electrophoretic current of the capillary unit. Capillary electrophoresis apparatus comprising a control unit for the purpose.

Images