JP2001228158A - Automatic analyzer and automatic analysis method - Google Patents

Abstract

(57) [Summary] [PROBLEMS] An automatic analyzer of a type in which a transport rack on which a sample is placed is transported and measurement is advanced, and the same item can be repeatedly measured from the same sample container any number of times. A new function called an evaluation mode is added, and a sample rack number 303 and a position number 304 are added to input information of a measurement request in addition to a sample number 302 so that the same sample rack number and the same position number can be input. Thus, the same item can be repeatedly measured from the same sample container. The same sample ID number 401 can be input to the input information of the measurement request, and the same item can be repeatedly measured from the same sample container. When inputting a measurement request, a plurality of the same measurement items can be requested for one sample. Then, on the screen displaying the basic performance measurement items, basic performance measurement such as simultaneous reproducibility was instructed.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an analysis such as a biochemical test and an immunoserologic test, and more particularly to an automatic analyzer for setting a sample container in which various types of samples are placed in a sample erection mechanism for measurement.

[0002]

2. Description of the Related Art A rack on which samples arranged by an operator of an automatic analyzer in a measurement request order or a measurement registration order are transported to a sample dispensing position of the analyzer, and after being positioned, a sample probe is subjected to a predetermined number of times. When the dispensing of one sample is completed and the dispensing of one sample is completed, the rack moves by one sample from the loading side to the storage side, and the dispensing operation of the next sample is started again. Thus, the same operation is repeated, and the sample container advances by one sample. Conventionally, when the dispensing operation for one sample is completed, the rack always advances by one sample to dispense the next new sample. When measuring the reproducibility of measured values with 100 measurements, 1
Samples to be placed in 00 sample containers and 100 sample containers were prepared. The reason for this is that, in order to minimize errors in the sample dispensing operation due to input errors of the request information by the operator, an input restriction that the same rack number / position number cannot be input continuously to the request information has been provided. .
Although there was a method of creating a screen dedicated to evaluation measurement, it was impossible with a conventional device because a memory was taken for operation control of the device and a usable memory capacity was limited.

[0003] Japanese Patent Application Laid-Open No. 9-96643 describes that when dispensing the same sample into a microplate, the same liquid suction auxiliary tube is used.

[0004]

The samples used to control the accuracy of the apparatus which can be placed in 100 sample containers are expensive, and thus a large number of expensive samples must be prepared. The reason for this is that, in order to minimize errors in the sample dispensing operation due to input errors of the request information by the operator, an input restriction that the same rack number / position number cannot be input continuously to the request information has been provided. . There was also a method of creating a screen dedicated to evaluation measurement, but with the conventional device,
This was not possible because memory was used to control the operation of the device, and the available memory capacity was limited.

[0005] Since automatic analyzers for clinical tests are required to maintain a high level of measurement accuracy, reproducibility and accuracy are regularly checked in a laboratory. In particular, at the time of installation of the apparatus, the check work of the apparatus maker, the reagent maker and the inspection facility operator is performed for several weeks.
Conventionally, it takes time and labor for experiments such as setting sample containers,
Checking for reproducibility took a long time. There were many work mistakes due to the labor involved. In addition, a large amount of waste such as used sample containers and quality control samples was generated.

The present invention has been made in view of the above-mentioned points, and enables the repetitive dispensing of a sample from the same sample container without complicating the structure of the apparatus. Simultaneous reproducibility measurement, linearity measurement, effect of coexisting substances in sample, sample probe
An object of the present invention is to provide an automatic analyzer and an automatic analysis method that can easily perform operations such as the presence or absence of carry-over of a reagent probe, a stirring rod, and a reaction container.

[0007]

In order to solve the above problems, the present invention has the following arrangement.

A screen for basic performance test items is added to the routine operation screen, and an experiment selection button for simultaneous reproducibility, linearity, coexisting materials, carryover of a stirring rod, carryover of a reaction vessel, carryover of a reagent probe, and carryover of a sample probe. Is provided, and by pressing this button, a screen for setting conditions for each experiment appears, and request information can be input.

When a clinical laboratory technician brings a sample for evaluation of measurement accuracy, continuous measurement from the same sample container cannot be performed in the measurement mode of a general sample. Therefore, the operator changes the screen to the evaluation mode, and changes the screen to the evaluation mode. Request measurement from. The request information is stored in the memory, and the operator sets the rack on which the sample is placed and starts the apparatus. The apparatus first reads the rack number or the sample ID, and detects the presence or absence of a sample container. Rack number or sample ID read
Is matched with the request information stored in the memory, the items are sampled according to the request information. In conventional equipment,
The same rack number, position number, and sample ID could not be continuously input to the input area for the rack number, position number on the rack, and sample ID. In the present invention, the same number can be input to the input area for the rack number, position number, or sample ID.

The sample probe simply repeats the sample dispensing operation at regular intervals, and the conveyor belt positions the rack. Therefore, when it is desired to repeat the dispensing operation of the same item from the same sample container, the same rack number is used.・ If the same position number is input from the screen for the required number of samples, the sample dispensing operation can be repeated from the same position number any number of times.

Normally, the rack advances only from the rack input side to the rack storage side. However, the rack can be sandwiched between two claws and held in the opposite direction to enable the movement in the opposite direction, thereby enabling the sample carryover. The sample can be reused for repeated measurement of the same pattern in which a low concentration sample is measured several times after a high concentration sample like measurement.

The rack usually loaded is discharged when the measurement for one rack is completed, and the next rack is introduced into the sample dispensing area and positioned. In the present invention, for example, when one rack has five sample racks and only one rack
If the rack number designation of the sample is the same, the rack is returned to the input side using the retest line, and the sample at the first position of the rack is recognized and measured as the sample of the sixth sample. The same rack number is continuously input for the desired number of measurements, so that the rack can be circulated. A plurality of racks having different rack numbers can be circulated by interrupting racks having different rack numbers.

[0013] In order to facilitate repeated measurement of the same sample in the same sample container, a holder is provided above the transport line in the sample dispensing area of the apparatus, in which a container capable of holding a large amount of sample at a time is provided. A large number of samples were set in the sample so that the same sample could be repeatedly measured.

[0014]

Embodiments of the present invention will be described below with reference to the drawings.

FIG. 1 is a diagram showing a method of transporting and storing a rack of the automatic analyzer shown in the present embodiment, and its basic operation will now be described.

The automatic analyzer comprises a loading section 1 provided with a sample erection device which is a plurality of racks, two analyzers 2 provided in a sample dispenser and a reagent dispenser, an analyzer 3, a transport line 4, and It is composed of a rack storage section 5. Each rack has a plurality of positions in which sample containers are placed.

A sample is placed in a sample container 6 and set on a rack 7. The rack 7 is mounted on the rack input unit 1. When the start key is pressed after inputting and registering the analysis items of each sample 6, the apparatus pulls the rack with the claw for conveyance and starts the rack conveyance following the reset operation. The rack number or sample ID of the rack 7 sent out from the rack insertion unit 1 is read by the rack number or sample ID reader 10. The apparatus determines whether a rack corresponding to the input information has been input, and when the corresponding rack arrives, transports the rack to the sample dispensing area. The first sample among a plurality of samples placed on the rack is positioned at the sample dispensing position on the main line 8. The sample in the sample container 6 is dispensed, discharged by a set amount to the reaction container, and the dispensing and discharging are repeated by the requested number of tests. The rack from which the sample has been collected is collected in the rack storage unit 5, and the measurement is completed.

FIG. 2 shows a flowchart of a process from receiving a sample to completing the measurement.

The operator sets a sample to be measured (S101). According to the measurement purpose of the sample to be measured, the measurement mode is selected from "Urgent", "General", and "Evaluation" on the device screen (S102), and request information unique to each sample is input. The sample type (S103) indicating whether the sample is serum, urine, or other, the sample number (S104) which is the serial number of the sample, and the rack number (S
105), the position number (S106), the cup type (S107) representing the type of the sample container, and the measurement request item (S108) are sequentially input, and the apparatus is started (S1).
09). The device reads the rack number and the sample ID (S1
10) The presence or absence of the sample container is detected (S111), and the sample is dispensed (S113). During the sample dispensing operation, the rack number and the position number of the next sample number are checked. If the rack number and the position number input value are the same (match), the rack is not fed (S112). The sample is stopped at the same position, and the sample dispensing operation is performed for the sample at the same position (S113). The measurement ends with the output of the result (S114).

FIGS. 3, 4 and 5 show examples of screens of the device which serve both to switch the measurement mode and to input request information. FIG. 3 shows an example in which the rack mode 303 and the position number 304 are used for identifying the sample, and FIG. 4 shows an example in which the sample ID number 401 is used for identifying the sample. Further, when requesting a measurement item, a method in which a plurality of the same items cannot be requested for one sample has conventionally been adopted. However, in FIG. 5 which is an embodiment of the present invention, the software is changed and the same Be able to request the same item multiple times for the sample,
An example of a screen that enables repeated measurement of the same item is shown.

A specific description will be given using the screen example of FIG. AST was measured 5 times from position number 1 of rack number 1 for sample numbers 1 to 5, and AST was also measured 5 times from position number 2 for rack number 1 for sample numbers 6 to 10. When measuring twice, enter the following from the screen. First, a sample type 301 is input, and then, a sample number 302, a rack number 303, and a position number 304 are input. In the order of sample number-rack number-position number, sample number 1 is 1-1-1, sample number 2 is 2-1-1, and 3
-1-1,4-1-1,5-1-1,6-1-2,7-
1-2, 8-1-2, 9-1-2, 10-1-2. Request AST (aspartate aminotransferase) with all measurement request item selection keys 305. Finally, a cup type 306 used for measurement is input. When the rack with the rack number 1 is loaded, the apparatus positions the position number 1 of the rack with the rack number 1 in the sample dispensing area, and moves the sample probe to the position of the rack number 1 for AST measurement. Dive into the No. 1 sample container 5 times. At the intervals of the fifth and sixth dispensing operations, the rack is moved by one sample in the moving direction and positioned, and the AS number is five times from the position number 2 of the rack number 1.
The dispensing operation of T is performed. Conventionally, when AST is dispensed once with one sample, the rack is forcibly moved by one sample.
Although two sample containers were required, the measurement of 10 tests can be performed with the two sample containers placed in No. 1 and No. 2, which saves labor for experiments and saves sample containers.

As shown in FIG. 4, the rack number 30 shown in FIG.
The same measurement can be performed by using the sample ID number 401 instead of 3 and the position number 304.

FIG. 5 shows an example in which the same sample is requested a plurality of times, for example, AST, as described above.

The contents of the measurement request are as follows.

AST: aspartate aminotransferase ALT: alanine aminotransferase LD: lactate dehydrogenase TP: total protein ALP: alkaline phosphatase GLU: glycose ALB: albumin T-BIL: total bilirubin D-BIL: direct bilirubin GGT: γ-glutamyltrans Peptidase LAP: Leucine aminopeptidase MG: Magnesium IP: Inorganic phosphorus FE: Iron CHE: Cholinesterase T-CHO: Total cholesterol F-CHO: Free cholesterol BUN: Urea nitrogen PL: Phospholipid HDL-C: HDL-cholesterol TTT : Thymol opacity test ZTT: Zinc sulfate opacity test NA: Sodium K: Potassium CL: Chlorine CU: Copper CRP: C-reactive protein RF: Rheumatism Child

FIG. 6 shows a modification of the rack transport system of the automatic analyzer shown in this embodiment.

In the rack transport system described above, the rack 7 only moves from the loading side to the storage side. In this example, the rack 7 can be moved from the loading side to the storage side and from the storage side to the loading side. ing. The rack is conveyed by being sandwiched between claws from both sides by a rack holder 16 connected to a conveyor belt 15 so that the rack can perform a backward movement. According to the present embodiment, when it is necessary to measure two or more different concentration samples as in the measurement of sample carryover, the sample used once for measurement and passed through the sample dispensing position is returned to the sample dispensing position again. Measurement.

FIG. 7 shows another modification of the rack transport system of the automatic analyzer shown in this embodiment.

The operator sets the rack on which the sample is placed in the rack input section 1 and starts the apparatus. The measurement of the first rack is performed, and the measured rack once the sample dispensing is completed is circulated using the retest line 11 for the retest sample provided in the apparatus and is measured again. Therefore, the same sample can be continuously measured.

The rack 7 positioned in the sample dispensing area
Is dispensed in accordance with the request information, returned to the main line 8 after the measurement, returned to the re-inspection line 11 for the re-inspection sample, returned to the rack input section 1, and received the rack number or the rack number by the sample ID reader 10. Alternatively, after reading the sample ID again, the sample is positioned again in the sample dispensing area and dispensing is performed. The rack for which all the requested measurements have been completed is stored in the rack storage unit 5. Since the same sample container can be used repeatedly for measurement, the labor of the experiment is omitted, and the number of used sample containers is reduced.

FIG. 8 shows a modification of the sample measuring method of the automatic analyzer shown in this embodiment. In this example, a continuous measurement sample container containing a large amount of sample, particularly a sample container 14 dedicated to continuous measurement, and a sample container holder 13 for setting this container are provided above the sample dispensing position of the apparatus. The present sample container holder 1
When a sample is set or filled in the sample container 14 for continuous measurement at 3, it is detected that the container has been set, and the apparatus recognizes that it has entered the evaluation mode. When the start switch is pressed, the apparatus performs measurement according to the request information for evaluation measurement input by the operator. Since there is no need to prepare any sample containers, it is possible to save labor for experiments.

Next, measurement of the basic performance of the apparatus will be described.

FIG. 9 is a flowchart for measuring the simultaneous reproducibility.

The simultaneous reproducibility measurement is a type of basic performance measurement, and is a test for checking the reproducibility (mean value, standard deviation, coefficient of variation) of the measured values when the number of samples is about 20 or more.

The operator selects a measurement mode “reproducibility”, which is one of the basic performance measurement items, on the basic performance measurement screen of the apparatus (S901), the sample type (S902), the cup type (S903), and the number of measurements ( The N number) (S904), the number of samples (S905), and the measurement item (S906) are sequentially input. Prepare and set the rack on which the sample containers are placed (S
907), the apparatus is started (S908). The apparatus reads the rack number and the sample ID (S909), detects the presence or absence of a sample container (S910), and dispenses the sample (S91).
1). The measurement ends with the output of the result (S91).
2). When the reproducibility calculation key is pressed after the measurement is completed, the reproducibility result is automatically calculated and displayed (S913).

FIG. 10 shows an example of a screen for inputting measurement conditions for simultaneous reproducibility measurement. The operation will be specifically described using this screen example.

The reproducibility of AST was measured by the number of measurements (N number) of 20
In the case of measuring with two samples each time, input as follows from the screen. First, “reproducibility” is selected with the selection key 1001. A sample type 1002 and a cup type 1003 are input. Next, enter 20 for the number of measurements (N number) 1004 and 2 for the number of samples 1005, and use the measurement item selection key 1006 to select A.
When the ST is requested, the apparatus automatically calculates and recognizes that the dispensing operation is performed ten times from one sample. The sample containers are set in order from position 1 of the rack, and the rack is installed on the apparatus. When the start key 1007 is pressed, the device starts measurement. The sample probe plunges 10 times into the sample container at position 1 of the rack transported to the sample dispensing position for AST measurement. Thereafter, the rack moves in the advancing direction by one sample to perform the dispensing operation 10 times at position 2 for AST measurement. Thereafter, the rack is stored in the rack storage unit. When the reproducibility calculation key 1008 is pressed after the measurement is completed, the reproducibility result is automatically calculated and displayed. Conventionally, when the dispensing operation of one sample is completed, the rack is forcibly moved by one sample, so that 20 sample containers are required. However, if this method is used, measurement of 20 tests can be performed with two samples. As described above, even if the sample number is changed, by having the function of measuring the same item from the same sample container, the labor of the experiment is omitted, and the waste such as the used sample container is reduced.

FIG. 11 is an example of a screen for inputting measurement conditions for linearity measurement. The operation will be specifically described using this screen example. The linearity test is a test for confirming to what extent the measured value of the device has linearity as the concentration of the target substance gradually increases.

First, the operator operates the selection key 1 on the apparatus screen.
At 101, "linearity" is selected. Next, as measurement conditions, the number of series to be measured is 1102, and the concentration of each sample is 1103a.
~ J, sample type 1104 of each sample, sample number 1105,
The rack number 1106, the position number 1107, the cup type 1108, and the measurement item 1109 are sequentially input. Set the sample to be measured and set the start key 11
Press 10. The device reads the rack number and sample ID,
The presence or absence of the sample container is detected, and the sample is dispensed. With respect to the first sample, the rack position is fixed and the dispensing operation is performed until the dispensing operation of all the requested items is completed. After the dispensing operation of this sample is completed, the apparatus performs rack position feed for one sample. Continue dispensing operation until there is no unmeasured sample. The measurement ends with the output of the result. Data output is performed on the screen and prints all measured values and calculated values of each series. By pressing the result confirmation key 1111, a linearity graph is displayed on the screen.

As described above, by having the function of measuring the same item from the same sample container even when the sample number changes, the labor of the experiment is saved and the waste such as the used sample container is reduced.

FIG. 12 shows an example of a screen for inputting measurement conditions for a coexisting substance test. The input method and operation will be specifically described using the screen example. The coexisting substance test is used to measure the degree of influence of coexisting substances. This is a test to confirm whether or not the above is affected. In general, there are four typical substances that interfere with this reaction in the test items of the clinical test, namely, intralipos (a chyle component), ascorbic acid, hemoglobin (a hemolytic component), and bilirubin (a jaundice component). In the measurement, the coexisting substance and the sample were gradually (0/10 to 10/1)
Analyzing 11 kinds of samples, usually prepared by mixing in 0),
The degree of interference is confirmed by comparing the relationship between the amount of coexisting substance and the value of the target substance. Furthermore, the sample whose concentration has been adjusted in several steps is measured a plurality of times to check for variations.

The operator operates the selection key 120 on the apparatus screen.
Select "coexisting substance" in step 1. Next, as measurement conditions,
Number of series 1202 and coexisting substance concentration of each sample 1203a
~ J, sample type 1204 of each sample, sample number 1205,
In the rack number 1206, position number 1207, cup type 1208, and measurement item input area, measurement items 1209 are sequentially input. The rack on which the sample to be measured is placed is set, and the start key 1210 of the device is pressed. The apparatus reads the rack number and the sample ID, detects the presence or absence of the sample container, and dispense the sample. With respect to the first sample, the rack position is fixed and the dispensing operation is performed until the dispensing operation of all the requested items is completed. After the dispensing operation of this sample is completed, the rack position is fed for one sample. Continue dispensing operation until there is no unmeasured sample. The measurement ends with the output of the result. In outputting data, all measured values of each series are displayed on a screen and printed on a printer. By pressing the result confirmation key 1211, a graph of the coexisting substance is displayed on the screen.

When a measurement request is made on the screen shown in FIG.
For a sample to be measured a plurality of times, the same rack number is input to the rack number input area and the same position number is input to the position number input area, and the same item from the same sample container is repeatedly measured. Taking an example in which the number of measurements (N number) of a rack capable of mounting five samples on one rack is 5, the sample numbers 1 to 5 are all set to the rack number 1 and the position number to 1 in FIG. Select items such as AST, ALT, LD,.

Subsequently, for the sample numbers 6 to 10, all the rack numbers are 1 and the position numbers are 2, and the same items as above are selected.

Hereinafter, sample numbers 11 to 15 are rack number 1 and position number 3, sample numbers 16 to 20 are rack number 1 and position number 4, sample numbers 21 to 25 are rack number 1, position number 5, and sample number 26. 30 to rack number 2, position number 1, sample numbers 31 to 35 are rack number 2, position number 2, and sample numbers 36 to 40 are rack number 2, position number 3, and sample numbers 41 to 45.
Is rack number 2, position number 4, sample number 46-5
0 is rack number 2, position number 5, sample number 51-
Reference numeral 55 designates rack number 3 and position number 1 after each item selection, dispenses the sample whose concentration has been adjusted in 11 steps into cups, sets the sample on the designated rack, and starts analysis. As described above, even if the sample number is changed, by having the function of measuring the same item from the same sample container, the labor of the experiment is omitted, and the waste such as the used sample container is reduced.

The carryover test of the stirring bar is performed to determine whether the reagent component adhered to the surface of the stirring bar is carried over to the reaction solution for the next item measurement even after the washing and affects the measured value of the next item. This is a test to check how much the measured value is given.

For example, when measuring carryover of the stir bar from item A to item B, the same procedure is performed to see how much the reagent component attached to the stir bar and carried over to the next test affects the next item. Samples with values must be measured continuously.

Normally, the operator measures the average value of the item B under the condition of not receiving the carry-over in about 30 times of measurement,
Further, the measurement of the B item after the A item is repeatedly performed like "AAA / BBB / AAA / BBB ...". The measured value of item B immediately after item A is compared with the average value of item B, and if there is a significant difference, it is determined that there is an influence.

The operation of the apparatus when measuring carryover of the stirring bar will be described with reference to the screen example of FIG. 13 and FIG.

The operator operates the selection key 1301 in FIG.
Use to select “Stir bar carryover”. Next, the operator selects the sample type 1302, cup type 1303,
Number of samples 130 means number of sample containers used for measurement
4, the number of measurements 1305 of the reference value of the measurement item on the affected side, the measurement item name 1306 on the influencing side and the number of consecutive tests 1307, the measurement item name 1308 on the affected side and the number of consecutive tests 1309, the number of measurement rounds 1310 for checking the influence from the item A to the item B is sequentially input. The apparatus divides the total number of tests by the number of samples used for measurement, and automatically calculates and recognizes the number of dispensing from one sample container.

The operator sets the rack 7 on which the quality control sample 6 shown in FIG. FIG.
When the start key 1311 of the device is pressed, the rack 7
After the rack number or the sample ID is read by the rack number or the sample ID reader 10 and the presence or absence of the sample container on the rack is detected, the main line 8 is followed and the analysis unit 2,
3 and positioned at the sample dispensing position. Until the position number is changed, the sample is dispensed from a sample container having one sample lobe in accordance with the request information of AAABBB... As for the data output, all the measured values of each test are displayed on the screen and printed on the printer at the same time. By pressing the result confirmation key 1312 in FIG. 13, the calculated value of the carry-over rate is displayed on the screen and printed on the printer.

As described above, even if the sample number is changed, by having the function of measuring the same item from the same sample container, the labor of the experiment can be saved, and the waste such as the used sample container can be reduced.

In the carry-over test of the reaction vessel, when the reagent component adhered to the inner surface of the reaction vessel which is repeatedly used for the measurement of different items even after washing is carried over to the reaction solution of the next item, the measured value is affected. It is a test to confirm whether or not to give.

For example, when measuring the carryover of the reaction vessel from item A to item B, it is checked how much the reagent component attached to the inner surface of the reaction vessel and carried over to the next test affects the measured value of the next item. Therefore, samples having the same value must be continuously measured.

Normally, the operator first requests reproducibility measurement at a measurement count of 30 or more to measure the reference value of item B, and requests measurement of item A over about 10 tests in order to contaminate the reaction vessel with the reagent of item A. . In the next round, measure the B item in the reaction vessel group that measured the A item, and observe the carryover. Therefore, the first A item measurement group so that the B item comes exactly in the first reaction vessel of the A item reaction container group. The dummy item is requested from the next test after the last test to the test immediately before the B item measurement which receives the carryover of the next round.
If the measured value of item B measured in the reaction vessel in which item A was measured is compared with the reference value (average value) of item B having about 30 measurements, if there is a significant difference, it is determined that there is an influence.

An operation example of the apparatus when measuring the carryover of the reaction vessel will be described with reference to the screen example of FIG. 14 and FIG.

The operator operates the selection key 1 on the screen shown in FIG.
At 401, "reaction vessel carryover" is selected. Next, the operator selects the sample type 1402 and cup type 14 of the measurement conditions.
03, 14 samples, which means the number of sample containers used for measurement
04, the number of measurement 1405 of the reference value measurement of the measurement item of the affected side, the measurement item name 1406 of the affected side and the number of consecutive tests 1407, the measurement item name 1408 of the affected side and the number of consecutive tests 1409, the item name 1410 of the dummy item for filling the interval is sequentially input. The apparatus divides the total number of tests by the number of samples used for measurement, and automatically calculates and recognizes the number of dispensing from one sample container.

The operator sets the rack 7 on which the quality control sample 6 shown in FIG. FIG.
When the start key 1411 is pressed, the rack 7 reads the rack number or sample ID by the rack number or sample ID reader 10 and detects the presence or absence of a sample container on the rack. , 3 and positioned at the sample dispensing position. Until the position number on the rack is changed, the sample lobes are stored from one sample container in the B item group, the A item group, the dummy item group, and the B item group.
In the order of the item group, 30 items for B items, 10 tests for A items, dummy items for filling the reaction vessel from the test following the last test of this A item group to the test immediately before the second B item group, Further, the sample is dispensed in the order of 10 tests of B item affected by the A item. For data output, all measured values of each test are displayed on a screen and printed on a printer.
By pressing the result confirmation key 1412, the carry-over calculation value is displayed on the screen and printed on the printer.

As described above, even if the sample number is changed, by having the function of measuring the same item from the same sample container, the labor for the experiment can be saved, and the waste such as the used sample container can be reduced.

As described above, in the automatic analysis method of sequentially measuring a plurality of samples by dispensing a sample and dispensing a reagent, a measurement sample item, a requested measurement item, and a basic performance measurement item are displayed on a screen. When dispensing a sample at the same position in the same rack multiple times, instruct the sample to be measured and the contents of the requested measurement on the screen, save this, change the screen, and change the basic performance measurement item to the next basic performance. Measurement,
That is, measurement of reproducibility of measurement value, measurement of linearity of measurement value, measurement of influence of coexisting substance, carryover measurement of stir bar, carryover measurement of reaction vessel, carryover measurement of reagent probe, and carryover measurement of sample probe. The present invention provides an automatic analysis method for instructing, setting the rack at the same position, and dispensing a sample at the same position.

The carry-over test of the reagent probe is carried out to determine whether or not the reagent component adhering to the inner and outer surfaces of the reagent probe is carried over to the reaction solution of the next item even after the washing and affects the measured value of the next item. This is a test to check how much the measured value is given. In this case, the carry-over test of the reagent probe usually narrows down items to be given or given in the screening test, and carries out a carry-over confirmation test. In the embodiment of the present invention, a carryover confirmation test will be described.

For example, when measuring the carryover of a reagent probe from item A to item B, it is checked how much the reagent component attached to the inner and outer surfaces of the reagent probe and carried over to the next test affects the next item. Therefore, samples having the same value must be continuously measured.

Normally, the operator measures the average value of the item B under the condition that the carry-over is not performed in about 30 measurement times,
Further, the measurement of the B item after the A item is repeatedly performed like "AAA / BBB / AAA / BBB ...". The measured value of item B immediately after item A is compared with the average value of item B, and if there is a significant difference, it is determined that there is an influence.

An example of the operation of the apparatus when measuring carryover of a reagent probe will be described with reference to the screen example of FIG. 15 and FIG.

The operator selects “reagent probe carryover” with the select key 1501 indicating the carryover R and the probe on the screen of FIG. Next, the operator applies the sample type 1502 of the measurement conditions, the cup type 1503, the number of samples 1504 indicating the number of sample containers used for measurement, the number of measurements 1505 at the time of measuring the reference value of the measurement item on the affected side, and gives an effect. The measurement item name 1506 on the side and the number of consecutive tests 1507, the measurement item name 1508 on the affected side and the number of consecutive tests 1509, and the number of rounds 1510 for observing the influence from item A to item B are sequentially input. The apparatus divides the total number of tests by the number of samples used for measurement, and automatically calculates and recognizes the number of dispensing from one sample container.

The operator sets the rack 7 on which the quality control sample 6 shown in FIG. FIG.
When the start key 1511 of the rack 7 is pressed, the rack number or sample ID is read by the rack number or sample ID reader 10, and the presence or absence of a sample container on the rack is detected. , 3 and positioned at the sample dispensing position. Until the position number on the rack is changed, the sample lobe dispenses the sample from one sample container according to the request information of B items × 30 times, AAABBBB. For data output, all measured values of each test are displayed on a screen and printed on a printer. FIG.
By pressing the result confirmation key 1512, the calculated value of the carry-over rate is displayed on the screen and printed on the printer.

As described above, even if the sample number is changed, by having the function of measuring the same item from the same sample container, the labor of the experiment is omitted and the waste such as the used sample container is reduced.

The carryover test of the sample probe is as follows.
After washing, the sample components that adhere to the inner and outer surfaces of the sample probe are carried over to the reaction solution for the next sample measurement item, and affect the measured value of the next item, and if so, how much. This is a test to confirm.

Normally, the operator continuously measures the high-concentration sample containing the high-concentration target substance three times, and successively removes the low-concentration sample containing no measurement target substance and having a measured value of zero.
Measure continuously. A low-concentration sample having a measured value of zero is measured in advance at about 30 measurement times, and the average value (reference value) is compared with the measured value of the low-concentration sample immediately after measuring the high-concentration sample. If there is a difference, it is determined that there is an effect. The carry-over rate of the sample probe is obtained by dividing the difference between the reference value and the measured value of the low concentration sample immediately after measuring the high concentration sample by the concentration of the high concentration sample. Since the concentration of a high-concentration sample cannot be usually measured as it is, it is measured after dilution.

An operation example of the apparatus when measuring carryover of the sample probe will be described with reference to the screen example of FIG. 16 and FIG.

The operator selects “sample probe carryover” with the select key 1601 indicating the carryover S and the probe on the screen of FIG. Next, the operator sequentially inputs the sample type 1602, the cup type 1603, and the measurement item 1604 for which the carryover is to be measured as the measurement conditions.
Further, a rack number 1605 in which a high-concentration sample is set, a position number 1606, the number of dispensations 1607, and a rack number 1608 in which a low-concentration sample to be carried over is set.
The position number 1609, the number of dispensing times 1610, the rack number 1611 in which the diluted sample diluted for measuring the concentration of the high-concentration sample is set, the position number 1612, and the number of dispensing times 1613 are sequentially input.

Take a five sample rack as an example. The operator
The rack number where the high-concentration sample (hereinafter referred to as sample A) is set is 1, the position number is 1, the dispensing frequency is 3, the rack number where the low-concentration sample saline (hereinafter sample B) is set is 1, and the position number is 2, 3 and 4, 1 for each dispensing frequency, 2 for the rack number where the diluted sample (hereinafter referred to as sample C) is set, 1, 2, 3, 4, 5 for the position number and 1 for each dispensing frequency Sample and rack number 1 according to the entered information.
The rack with the rack number 2 is set in the rack input unit 1.
When the start key 1614 is pressed, the rack 7 reads the rack number or sample ID with the rack number or sample ID reader 10 and detects the presence or absence of a sample container on the rack. , 3 and positioned at the sample dispensing position. Sample A at position No. 1 in the first rack is dispensed three times in succession, and samples B at subsequent positions Nos. 2, 3, and 4 are dispensed once for each sample container. Further, the sample C of the position numbers 1, 2, 3, 4, and 5 of the rack number 2 is dispensed once for each sample container, a total of 11 times, and the measurement is completed. Data output is
Display all the measured values of each test on the screen and print them on the printer. By pressing the result confirmation key 1615, the calculated value of the carry-over rate is displayed on a screen and printed on a printer.

As described above, even if the sample number is changed, by having the function of measuring the same item from the same sample container, the labor of the experiment is omitted, and the waste such as the used sample container is reduced.

[0074]

The present invention has the following effects.

According to the present invention, a screen having measurement items and basic performance measurement items is added, and the number of measurements of the same item is specified for the sample at the same position. In this case, the rack is stopped at the same position. Simultaneous reproducibility, linearity of measured values, influence of coexisting substances, sample probe, reagent probe, stir bar,
Basic performance measurement of the device, such as confirmation of the presence or absence of carryover of the reaction vessel, can be performed more easily than before. In addition, the efficiency of the operation is improved in that the operator can safely leave the apparatus. Since the number of sample containers to be handled can be reduced, the working time can be shortened, and working errors can be reduced.

Further, since the number of used sample containers and the consumption of the sample can be reduced, the waste of the used sample container and the sample itself, which may cause infection, can be reduced, thereby contributing to environmental problems.

[Brief description of the drawings]

FIG. 1 is an external view of an automatic analyzer having a function of repeatedly measuring the same sample.

FIG. 2 is a flowchart for repeatedly measuring the same sample.

FIG. 3 is a view showing an example of a measurement request screen of the automatic analyzer.

FIG. 4 is a diagram showing an example of a measurement request screen of the automatic analyzer.

FIG. 5 is a view showing an example of a measurement request screen of the automatic analyzer.

FIG. 6 is an external view of an automatic analyzer having a function of repeatedly measuring the same sample.

FIG. 7 is an external view of an automatic analyzer having a function of repeatedly measuring the same sample.

FIG. 8 is an external view of an automatic analyzer having a function of repeatedly measuring the same sample.

FIG. 9 is a flowchart for repeatedly measuring the same sample.

FIG. 10 is a view showing an example of a measurement request screen of the automatic analyzer.

FIG. 11 is a view showing an example of a measurement request screen of the automatic analyzer.

FIG. 12 is a view showing an example of a measurement request screen of the automatic analyzer.

FIG. 13 is a view showing an example of a measurement request screen of the automatic analyzer.

FIG. 14 is a view showing an example of a measurement request screen of the automatic analyzer.

FIG. 15 is a view showing an example of a measurement request screen of the automatic analyzer.

FIG. 16 is a view showing an example of a measurement request screen of the automatic analyzer.

[Explanation of symbols]

1 rack input section 2 analysis section D 3 analysis section P 4
Transport line, 5: rack storage section, 6: sample container, 7: rack, 8: main line, 9: retest buffer, 10 ...
Rack number or sample ID reader, 11: retest line, 12: remeasurement rack, 13: sample container holder,
14: Sample container dedicated to repeated measurement, 15: Conveyor belt, 16: Rack holder.

 ──────────────────────────────────────────────────続 き Continuing on the front page (72) Inventor Tomonori Mimura 882, Omo, Oaza-shi, Hitachinaka-shi, Ibaraki Prefecture Within the Hitachi Measuring Instruments Group (72) Inventor Mitsuo Hattori 1040, Omo-Ichi, Oaza-shi, Hitachinaka-shi, Ibaraki Co., Ltd. Hitachi Science Systems, Inc. (72) Inventor Masami Hayashi 1040 Ichimo Ichimo, Hitachinaka City, Ibaraki Prefecture Incorporated Hitachi Science Systems Co., Ltd. (72) Shinobu Usui 1040 Ichimo Ichimo, Hitachinaka City, Ibaraki Prefecture Hitachi Science Systems, Ltd. (72) Inventor Tomomasa Otaki 1040 Ichimo Ichihiro, Hitachinaka City, Ibaraki Prefecture Inside Hitachi Science Systems, Ltd. (72) Inventor Naoko Saito 1040 Ichimo Ichimo, Hitachinaka City, Ibaraki Prefecture F-term in Hitachi Science Systems, Ltd. Reference) 2G058 AA05 AA07 AA08 CB09 CB15 CB17 CF12 EA02 EA04 FA02 FB01 FB03 FB06 FB07 GC05 GC06 GD01 GD06 GD07 GE05 HA04

Claims (8)

[Claims] The present invention comprises a sample erection device, a sample dispenser, and a reagent dispenser having a rack on which a plurality of samples are installed, and a plurality of positions provided on one rack. In an automatic analyzer for measuring a plurality of samples one after another by dispensing, a measurement item including a measurement sample item and a requested measurement item, and a screen for displaying basic performance measurement items of the apparatus are provided. Specify the number of measurements and requested measurement items of the same item for the sample at the same position of the sample erection device, and instruct the basic performance measurement from the basic performance measurement item when dispensing the sample at the same position on the same rack An automatic analyzer characterized by: 2. The rack according to claim 1, wherein the rack is stopped at the same position when the rack number and the position number of the next sample number coincide with the rack number and the position number of the sample to be dispensed. An automatic analyzer characterized by performing sample dispensing on a sample. 3. A rack storage unit according to claim 1, wherein the rack number and the position number of the rack stored in the rack storage unit are the same as the rack number and the position number of the next sample number. An automatic analyzer, wherein the rack is moved to a rack input section of a sample erection apparatus. 4. The automatic analyzer according to claim 1, wherein the measured rack once the sample dispensing is completed is circulated using the retest line and measured again. 5. The automatic analyzer according to claim 1, wherein a measurement mode selection item is displayed on the screen, and the measurement mode is set by selecting one of the displayed selection items. 6. The method according to claim 1, further comprising a sample container dedicated to continuous measurement and a sample container holder for setting the sample container for continuous measurement, wherein a measurement mode item is automatically set when a sample is set in the sample container for continuous measurement. A featured automatic analyzer. 7. The screen according to claim 1, wherein the screen for displaying the basic performance measurement includes reproducibility, linearity,
One of the basic performance measurement item groups consisting of coexisting materials, carryover stirrer, carryover reaction vessel, carryover reagent probe, and carryover sample probe is displayed, and when any of the items is indicated, one of the following Measurements: measurement of reproducibility of measurement values, measurement of linearity of measurement values, measurement of influence of coexisting substances, measurement of carryover of stirring rods, measurement of carryover of reaction vessels, measurement of carryover of reagent probes, and measurement of carryover of sample probes A featured automatic analyzer. 8. An automatic analysis method for successively measuring a plurality of samples by dispensing samples and dispensing reagents, wherein a measurement sample item, a requested measurement item, and a basic performance measurement item are displayed on a screen, In dispensing the sample at the same position a plurality of times, the sample to be measured and the contents of the requested measurement are instructed on the screen, and this is stored and the screen is changed. From the basic performance measurement item, the next basic performance measurement, that is, Instructs one of the following: measurement of reproducibility of measurement value, measurement of linearity of measurement value, measurement of influence of coexisting substance, measurement of carryover of stirring bar, measurement of carryover of reaction vessel, measurement of carryover of reagent probe, and measurement of carryover of sample probe. An automatic analysis method comprising setting the racks at the same position and dispensing samples at the same position.