JP2001026518A - Preparation composition for external use for skin - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain the subject composition capable of preventing the skin aging caused by active oxygen by formulating a vegetable extract excellent as a cosmetic additive from aspects of cost, irritation and effects and having inhibiting actions on the active oxygen. SOLUTION: This composition is obtained by including a fractionated substance prepared by treating an extract from an olive leaf with water or an ethanol solution by a column chromatographic method using a synthetic polymeric or a bound type silica gel packing as an essential ingredient. The fractionated substance is obtained by directly pulverizing, e.g. the live or the dried olive leaf, extracting the leaf with a suitable solvent at room temperature or under heating, distilling off the solvent, concentrating the resultant extract to 3-50 times, converting the concentrate into a 0-10% ethanol solution, providing a sample for filling, washing the filled sample with water or a 20% ethanol using the packing having 20-200 μm particle diameter and conducting the elution with a 30-60% ethanol solution. The prepared fractionated substance is preferably contained in an amount of 0.00001-5.0 wt.% expressed in terms of a dried solid in the objective composition.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD [0001] The present invention relates to a method for preparing an olive leaf extract by adding a column chromatographically treated fraction.
The present invention relates to a skin external preparation composition that eliminates active oxygen generated in skin due to ultraviolet rays and metabolism and prevents aging of the skin due to active oxygen.

[0002]

2. Description of the Related Art The skin is the organ most exposed to active oxygen because of its contact with air and irradiation of ultraviolet rays as well as oxygen stress caused by the body. On the skin surface, active oxygen oxidizes sebum, causing deterioration of the skin surface condition. It is also said that in the dermis, collagen and elastin, which are constituent components, cause a decrease in elasticity due to cross-linking, and that hyaluronic acid has a low molecular weight, thereby causing a decrease in moisturizing ability. Such a change in the dermis component has been proposed as a cause of wrinkles. In addition, active oxygen is considered to be deeply involved in the generation of spots, and is therefore presumed to be the main cause of various skin aging. For these reasons, cosmetics that protect the skin from active oxygen have been developed. Among them, many active oxygen suppressing substances have been identified from natural products. Sesameol in sesame oil, sesamol, tocophenol in rice germ, oryzanol, caffeic acid in coffee beans, carnosol in rosemary, rosemary acid, curcumin in turmeric, and many other plant extracts. The inhibitory effect was confirmed, and it has been blended in cosmetics and the like.

On the other hand, the olive leaf according to the present invention is Ol
It is described in the CTFA (Eighth Revision) as ive Leaf Extract and is widely used as a cosmetic additive. However, these extracts are extracted with ethanol, polyhydric alcohol or a mixture of these and water, and a high active oxygen elimination effect cannot be obtained. "In addition, olive leaf extract has antioxidant effect and oleuropein which is a component of olive leaf,
It is known that the hydrolyzate Hydroxytyrosol has a similar antioxidant effect (Phytoche
mistry, Vol. 31, No. 4, pp. 1173-1178, 1992), Hydro
A method for synthesizing xytyrosol has also been established (Phytoc
hemistry, ibid.) 』

[0004]

The isolated components in natural products which have been effective so far have the disadvantage that they are expensive and have strong skin irritation, and they are expensive, irritating and effective when formulated into cosmetics. There is a problem from the point of view. On the other hand, conventional plant extracts having an active oxygen-suppressing effect are weak in effect, and in order to obtain a sufficient effect, it is necessary to mix a large amount of the extract with cosmetics. However, it was not possible to obtain a preferable one in terms of the above.

[0005] Accordingly, an object of the present invention is to incorporate a plant extract having an excellent active oxygen suppressing effect as a cosmetic additive in view of price, irritation and effect, and to prevent skin aging caused by active oxygen. It is to provide a skin external preparation composition.

In such a situation, the present inventors have:
As a result of intensive studies on plant extracts that exist widely in the natural world, the present inventors have found that the olive leaf extract fraction has a high active oxygen-suppressing action, and have completed the present invention.

[0007]

That is, the present invention provides a skin external preparation composition having an active oxygen scavenging action, comprising a fraction obtained by subjecting an olive leaf extract to column chromatography. is there.

The present invention is constituted by the following claims 1 to 3. Claim 1: A water or ethanol solution extract of olive leaves is treated by a column chromatography method using a synthetic polymer or bonded silica gel filler, and the obtained fraction is used as an essential component. Skin external preparation composition. In a preferred embodiment, the eluate in the column chromatography has an ethanol concentration of 30 to 60 vol%. Claim 3: The skin external preparation composition according to claim 1 or 2, wherein the skin external preparation composition is a cosmetic having an active oxygen scavenging action.

Hereinafter, the skin external preparation composition of the present invention will be described in detail. In the present invention, the olive Olea
europaea L. A column chromatographically treated fraction of a leaf extract (Mentaceae) is used as an essential component.

The olive leaves used in the present invention are olives native to the Mediterranean region and North Africa, and are currently grown on relatively high temperature lands including the Mediterranean region. The olive leaves are oblong or needle-shaped leather and sharply pointed. "The components of olive leaves include secoiridoid glycosides oleuropein, demethyloleuropein,
oleoside, ligstroside, caffeic acid ester verbas
coside, rutine of flavonoid glycoside, luteolin glyco
sides, apigenin glycosides, oleano triterpene
lic acid, betulinic acid, triterpenol siteste
rol, α-amyrin, β-amyrin, uvaol, erythrodiol
And chlorophyll. Among these components, components having an active oxygen scavenging action are sequoyloid glycosides, caffeic acid esters, and flavonoid glycosides. 』

When preparing a column chromatographic fraction of the olive leaf extract, which is an essential component of the present invention, the raw or dried olive leaves may be used as they are or may be pulverized, if necessary. Organically grown leaves can be used as well. The method for preparing the extract is not particularly limited, and examples thereof include a method in which extraction is performed at room temperature or under heating using various appropriate solvents.

Specifically, water, lower monohydric alcohols such as methanol and ethanol, and one or a mixture of two or more thereof can be used as the extraction solvent. Among these, it is particularly preferable to use water or a 20 to 60% ethanol solution. If the ethanol concentration is too high, a large amount of components such as triterpene and chlorophyll are extracted, and post-treatment (concentration and the like) becomes difficult.

In the method of preparing the extract as a sample for column packing, the alcohol is distilled off by concentration to obtain a 3 to 50-fold concentrated solution. Although triterpenes are precipitated by concentration, they can be removed by filtration. The concentrated liquid can be used as a filling sample as a 0 to 10% ethanol solution.

As a column chromatography method, an open column can be used, but the use of pressurized liquid chromatography is industrially desirable. Specifically, low pressure, medium pressure, and flash chromatography can be used.

As the packing material used for column chromatography, reversed-phase bonded silica gel or a polymer-based packing material can be used. Specific examples include, but are not limited to, bonded type rishikagel such as ODS type and DMS (dimethyldichlorosilane) type, and high molecular type filler such as styrene-divinylbenzene type and methacrylate ester type. Not something. As the particle diameter of the filler, 20 to 2
One having a thickness of 00 μm can be used.

As the solvent used for elution, water, methanol, ethanol, acetone, n-propanol, ethyl ether, ethyl acetate, and one or a mixture of two or more of these can be used. In particular, it is desirable to elute with a 30 to 60% ethanol solution after washing a sample filled with water to 20% ethanol.

The eluate can be collected in small fractions or in large fractions. The collected eluate can be used as a fraction as it is, or its concentration can be increased as a concentrate. An extract powder can also be obtained by an operation such as spray drying or freeze drying. If necessary, column chromatography can be further repeated, or the fraction can be purified by countercurrent chromatography. These fractions can be prepared into a fractionation liquid, a fractionation powder, and a fractionation paste, and further formulated as appropriate for use.

The content of the olive leaf extract column chromatographic fraction, which is an essential component of the skin external preparation composition according to the present invention, is preferably 0.0000 in terms of dry solids.
1 to 5.0% by weight (hereinafter simply referred to as "%"), particularly preferably 0.001 to 0.5%. When a fraction is used, the extract concentration and the like are not limited at all if the content of the dry solid content as a solute is within the above range.

The composition for external use on the skin of the present invention may contain, in addition to the above essential components, antioxidants such as ascorbic acid, α-tocophenol, plant extracts such as rosemary extract, sage extract and clove extract. By doing so, the effect of eliminating active oxygen can be enhanced.

The composition for external use on the skin of the present invention contains surfactants, fats and oils, polyhydric alcohols, lower alcohols, thickeners, ultraviolet absorbers / scatterers, which are generally used as cosmetic ingredients. Preservatives, antioxidants, chelating agents, p
An H adjuster, a fragrance, a dye, and the like can be appropriately compounded.
Specific examples of these additional components are as follows.
Examples of the surfactant include anionic surfactants such as higher fatty acid soaps, alkyl sulfates, polyoxyethylene alkyl ether sulfates, acyl N-methyl taurate, alkyl ether phosphates, and N-acyl amino acid salts. Cationic surfactants such as alkyltrimethylammonium, dialkyldimethylammonium chloride, benzalkonium chloride, amino alcohol fatty acid organic silicone resin, alkyldimethylaminoacetic acid betaine, alkylamidodimethylaminoacetic acid betaine, 2-
Amphoteric surfactant such as alkyl-N-carboxy-N-hydroxyimidazolinium betaine, polyoxyethylene alkyl ether, polyoxyethylene branched alkyl ether, sorbitan ester, polyoxyethylene sorbitan ester, glycerin fatty acid ester, polyoxyethylene Glycerin fatty acid ester, polyoxyethylene cholesterol ester, polyoxyethylene hydrogenated castor oil fatty acid ester, glycerin alkyl ether, polyoxyethylene glycerin alkyl ether,
Nonionic surfactants such as polyglycerin fatty acid esters, and natural surfactants such as lecithin, lanolin, cholesterol, saponin and the like. As fats and oils, olive oil, camellia oil, macadamian nut oil, castor oil, sesame oil, safflower oil, vegetable oils such as peanut oil, pistachio oil, animal oils such as mink oil, egg yolk oil, jojoba oil, carnaubaroe, candelilla wax , Beeswax, waxes such as lanolin, liquid paraffin, paraffin, vaseline, ceresin, microcrystalline wax, hydrocarbons such as squalane, lauric acid, myristic acid, stearic acid, higher fatty acids such as isostearic acid, cetyl alcohol,
Stearyl alcohol, isostearyl alcohol, 2
Octyldodecanol, higher alcohols such as jojoba alcohol, isopropyl myristate, 2-octyldodecyl myristate, cetyl 2-ethylhexanoate,
Esters such as diisostearyl malate; silicone oils such as methylpolysiloxane and methylphenylpolysiloxane; Polyhydric alcohols include ethylene glycol, polyethylene glycol, propylene glycol,
1,3-butylene glycol, glycerin, polyglycerin, glucose, maltose, fructose, xylitol, sorbitol, maltotriose, erythritol and the like. Examples of the thickener include sodium alginate, xanthan gum, quince seed extract, guar gum, locust bean gum, sodium hyaluronate, collagen, casein, sodium carboxymethylcellulose, carboxyvinyl polymer and the like.

As the ultraviolet absorber, 2-hydroxy-
Benzophenone derivatives such as 4-methoxybenzophenone and 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid, paraaminobenzoic acid derivatives such as paraaminobenzoic acid and octyl paradimethylaminobenzoate, octyl paramethoxycinnamate and mono-diparamethoxycinnamate
Methoxycinnamic acid derivatives such as glyceryl 2-ethylhexanoate, and salicylic acid derivatives such as homomenthyl salicylate. Preservatives include benzoate, salicylate, sorbate, dehydroacetate, paraoxybenzoate, benzalkonium chloride, hinokitiol, resorcinol, ethanol and the like. Examples of antioxidants include tocophenol, ascorbic acid, butylhydroxyanisole, dibutylhydroxytoluene, and gallic esters. Examples of the chelating agent include sodium ethylenediaminetetraacetate, sodium polyphosphate, and citric acid.

Further, a plant extract having a physiological activity such as antibacterial activity, cell activation, moisturizing, suppression of sebum secretion, inflammation, promotion of blood circulation, astringency, antioxidation, whitening, etc., and a combined use of these extracted fractions and purified products. You can also.

Further, the skin external preparation composition of the present invention is not limited to general skin cosmetics, but includes quasi-drugs, cosmeceuticals and the like. The dosage form of the skin external preparation composition of the present invention may be any of a soluble type, an emulsifying type, a powdered dispersing type, etc., and the application is a basic cosmetic such as a lotion, an emulsion, a cream, a pack, and a makeup such as a foundation. It does not matter what kind of ingredients, eyeliner, bath agent, etc.

The composition for external use on the skin containing the thus obtained column chromatographic fraction of the olive leaf extract of the present invention as an essential component is excellent in active oxygen scavenging action, enhances skin function and prevents skin wrinkles. It has a high effect of making fine and firm skin and is useful as an antiaging cosmetic.

[0025]

Next, the present invention will be described with reference to examples, but the present invention is not limited to these examples.

(Production Example 1 of Column Chromatographic Fraction of Olive Leaf Extract)
Then, 5 kg of an ethanol solution was added thereto, and the mixture was stirred and extracted at 60 ° C. for 8 hours. The extract is concentrated under reduced pressure at 40 ° C. or less to about 500 g. An appropriate amount of ethanol and purified water are added to the concentrate to make 1000 g of a 10% ethanol solution, which is used as a column packing sample. A glass column having a diameter of 60 mm and a length of 1000 mm is filled with 2 L of a polymer adsorbent (Diaion HP-20, manufactured by Mitsubishi Chemical Corporation), and a column replaced with a 10% ethanol solution is filled with a packing sample at about 2 atm. After 5
Wash with L of 10% ethanol solution. After further washing with 3 L of a 20% ethanol solution, 50% ethanol 5
Elute with L and collect the eluate. The collected eluate is concentrated to 150 g under reduced pressure. 350 g of 1,3-
Butylene glycol is added to obtain a column chromatographic fraction A of an olive leaf extract having a dry solid content of about 1%.

(Production Example 2 of Column Chromatographic Fraction of Olive Leaf Extract) 30 v was added to 250 g of dried olive leaves.
Then, 5 kg of an ethanol solution was added thereto, and the mixture was stirred and extracted at 60 ° C. for 8 hours. The extract is concentrated under reduced pressure at 40 ° C. or less to about 500 g. An appropriate amount of ethanol and purified water are added to the concentrate to make 1000 g of a 10% ethanol solution, which is used as a column packing sample. A glass column having a diameter of 60 mm and a length of 1000 mm is filled with octadodecylsilylated silica gel for column chromatography, and a column replaced with a 10% ethanol solution is filled with a packing sample at about 2 atm. After washing, elute with 5 L of 60% ethanol and collect the eluate. The collected eluate is freeze-dried to obtain a column chromatographic fraction B of an olive leaf extract.

(Test Example 1) Measurement of active oxygen scavenging action SOD-like activity action The SOD-like activity was measured for column chromatography fractions A and B of olive leaf extract. SOD-like activity is NBT
(Method of Beauchemps et al. In combination with XOD system: Anal. Biochem., 44, 276-287, 19)
71). Table 1 shows the results.

[0029]

[Table 1]

(Test Example 2) Comparative Test with Existing Olive Leaf Extract The existing olive leaf extract was dried olive leaf 500
g to 70vol% 1,3-butylene glycol solution 5k
g, and the mixture was stirred and extracted at 60 ° C. for 8 hours. After cooling, about 4000 g obtained by filtration was used as a control. When the SOD-like activity was measured in the same manner as in the previous section, the activity was 51.3% at 10-fold dilution, which was 1/10 of the activity of the column chromatography fraction A.

(Test Example 3) Panel test The following external composition for the skin of the present invention and the external composition containing the existing olive leaf extract described in the preceding section were applied to 50 women aged 20 to 39 years (average age 24.5 years). Table 2 shows the sensory evaluation as a result of applying and using the control external composition twice daily (morning and evening) on the face for three consecutive months. Sensory evaluation was made on four items: moisture, texture, wrinkles, spots and freckles. Also,
As the olive leaf extract column chromatographic fraction used in the test, the fraction shown in Production Example 1 was used. As a control, a 70% 1,3-butylene glycol solution was used.

External composition for skin used in panel test Olive leaf extract column chromatographic fraction A 5.0% (or existing olive leaf extract) (control: 70% 1,3-butylene glycol solution) Glycerin 0% Ethanol 5.0% Paraoxybenzoate 0.2% Purified water 84.8%

As is evident from Table 2, the composition for external use on the skin of the present invention shows high efficacy, and the effect is derived from the olive leaf extract column chromatographic fraction of the present invention. became.

[0034]

[Table 2]

(Test Example 3) Acute toxicity test Sprague-Drawle of 5 males and 5 females fasted
y CD rats, males weighing 205-222 g, females 21
At 5 to 221 g, 8 to 12 weeks of age were used. The test sample is
The fractionation solution shown in Production Example 1 was used. Each rat was administered once by oral gavage at 2000 mg / kg based on the body weight after fasting at the time of administration. Rats were observed for death, or for obvious signs of toxicity, 30 minutes, 1 hour and 4 hours after administration, and once a day thereafter for 14 days. Body weight before administration on day 0, 7 days and 1
On day 4, individual recordings were made. At the end of the test, the rats were sacrificed by cervical dislocation and gross pathological examination was performed. No death or general toxicity symptoms were observed in the test results. Also, body weight by individual showed the expected weight gain in all rats throughout the study period. No abnormalities were observed at necropsy. By these, Sprague
-Acute oral 50% in DrawleyCD rats
The mortality (LD50) was confirmed to be greater than 2000 mg / kg body weight. This clearly indicates that the olive leaf extract column chromatographic fraction has excellent safety.

(Test Example 4) Skin safety test The fractionation solution shown in Production Example 1 was used as a test solution. The primary skin irritation test was performed using three New Zealand White rabbits, and as a result, the primary skin irritation index was 0.0. The continuous skin irritation test was performed on white Du of 3 males and 3 females.
Using nkin Hartley guinea pigs, 14
As a result of repeated administration for consecutive days, the maximum weekly average stimulation index was 0.0, and it was determined to be non-stimulated. The eye irritation test was performed on the eyes of three New Zealand White rabbits, and the maximum group average score was 0.0, which was determined to be non-irritant.
The skin sensitization test was performed using Adju using white guinea pigs.
By the vant / Patch method, one test animal and one control animal were used.
The test was performed by 0 mice, and the expression rate was 0/10, and it was confirmed that the animals had no sensitization. Phototoxicity and photosensitization tests
When performed using five Dunkin Hartley guinea pig females each, it did not cause any signs of phototoxicity or photosensitization. From these skin safety results,
It is clear that the olive leaf extract column chromatographic fraction is very safe in terms of skin safety.

(Test Example 5) Patch test 30 men from 20 to 51 years old (average age 25.2 years)
A total of 60 healthy volunteers, each consisting of 30 women, were used. As a test substance, the fraction solution of Preparation Example 1 and the composition for external use on skin used in the panel test were used.
A 0% 1,3-butylene glycol solution and physiological saline were used. In the test, a 24-hour blockage application test was performed. Using a fin chamber for human body sticking test (diameter 11 mm, Taisho Pharmaceutical Co., Ltd.), each test substance was applied in an amount of 0.1 mL, and then immediately stuck to the back of the subject and left for 24 hours.
After 24 hours, the fin chamber was removed and 30
After a minute and the next day (after 48 hours), the skin reaction was observed according to the criteria.

The criterion was in accordance with the criterion of the Japanese Patch Test Study Group. No response ・ ・ ・ ・ ・ (−) Slight erythema ・ ・ ・ ・ ・ (±) Obvious erythema ・ ・ ・ ・ ・ ・ ・ (+) Erythema + swelling ・ ・ ・ ・ ・ (++) Erythema + swelling + papule or small Bubble ・ ・ ・ ・ ・ (++++) Large bubble ・ ・ ・ ・ ・ (++++)

Table 3 shows the test results. No example showing a positive reaction (+ or more) was found. In addition, the case where slight erythema (±) occurred was 70% 1,3-
The frequency was almost the same as in the case of using butylene glycol solution or physiological saline. Therefore, the skin irritation was considered to be low, and it was clarified that there was no problem in terms of safety when blended into an external preparation for skin.

[0040]

[Table 3] : Physiological saline: 70% 1,3-butylene glycol solution: Fractionated solution of Production Example 1: Composition for external use of skin for panel test

(Example 1) Cream The following components (1) to (10) and, separately, the following components (11) to (14) are heated and dissolved at 75 ° C. to prepare solution A and solution B, respectively. Solution B was added to Solution A, emulsified, cooled to 50 ° C. with stirring, and component (15) was added to prepare a cream. (Components) (% by weight) (1) Jojoba oil 3.0% (2) Squalane 2.0% (3) Methylpolysiloxane 0.5% (4) Stearyl alcohol 0.5% (5) Cetyl alcohol 5% (6) glyceryl tri (capryl / caprate) 12.5% (7) glyceryl monostearate 5.0% (8) 1.5% diglyceryl monostearate (9) decaglyceryl monostearate 0% (10) Propyl parahydroxybenzoate 0.1% (11) Xanthan gum 0.1% (12) Olive leaf extract column chromatographic fractionation (Production Example 1) 5.0% (13) Methyl paraoxybenzoate 0 0.2% (14) Purified water 66.0% (15) Fragrance 0.1%

Example 2 Lotion The following components (5) to (8) were mixed and dissolved to obtain a solution A. Separately, the following components (1) to (4) and (9) were mixed and dissolved. The solution (B) was used, and the solution A and the solution B were uniformly mixed to obtain a lotion. (Ingredients) (% by weight) (1) Quince seed extract 8.0% (2) Glycerin 3.0% (3) 1,3-butylene glycol 5.0% (4) Olive leaf extract column chromatographic fraction (Production Example 1) 2.0% (5) Polyoxyethylene sorbitan laurate 1.2% (6) Ethyl alcohol 5.0% (7) Preservative 0.2% (8) Fragrance 0.1% ( 9) Purified water 75.5%

Example 3 Emulsion The following components (1) to (10), and separately from this, the following component (1)
Solution 1) to (14) and (16) were heated and dissolved at 75 ° C. to obtain solution A and solution B, respectively, and solution B was added to solution A, emulsified, and cooled to 50 ° C. with stirring to obtain component (15). Was added to prepare an emulsion. (Ingredients) (% by weight) (1) Jojoba oil 1.0% (2) Squalane 2.0% (3) Behenyl alcohol 1.0% (4) Tri (caprylic / capric acid) glyceryl 2.0% (5) Tetraglycerin condensed ricinoleic acid 0.1% (6) Propylene glycol monooleate 0.5% (7) Glyceryl monostearate 1.0% (8) Hexaglyceryl monomyristate 1.0% (9) Monomyristate Decaglyceryl 0.5% (10) Propyl parahydroxybenzoate 0.1% (11) Quince seed extract 5.0% (12) Olive leaf extract column chromatography fractionation product (Production Example 1) 3.0% (13 ) 1,3-butylene glycol 3.0% (14) Methyl paraoxybenzoate 0.1% (15) Fragrance 0.1% (16) Purified water 79.6%

Example 4 Cleansing Gel The following components (1) to (3), and separately from this, the following component (4)
(6) and (8) are heated and dissolved at 70 ° C. to obtain solution A and solution B, respectively, and solution B is added to solution A and stirred until uniform. While stirring, the mixture was cooled to 50 ° C., and the component (7) was added to prepare a cleansing gel. (Components) (% by weight) (1) Hexaglyceryl monomyristate 20.0% (2) Liquid paraffin 59.7% (3) Paraoxybenzoate 0.3% (4) Olive leaf extract column chromatography fractionation (Production Example 1) 5.0% (5) Concentrated glycerin 5.0% (6) Sorbitol 5.0% (7) Fragrance 0.1% (8) Purified water 4.9%

All of the above creams, lotions, emulsions, and cleansing gels had the same effects as the panel test shown in Test Example 3.

[0046]

As described above in detail, the olive leaf extract column chromatographically treated fraction, which is an essential component of the present invention, comprises:
It has an excellent active oxygen suppression effect, is excellent in safety, and a skin external composition containing it suppresses the generation of active oxygen causing aging of the skin, and is also excellent in safety. is there.

 ──────────────────────────────────────────────────続 き Continued on the front page F term (reference) 4C083 AA111 AA112 AA122 AC022 AC072 AC102 AC122 AC132 AC342 AC392 AC422 AC442 AD152 AD352 CC04 CC05 CC22 DD41 EE10 EE12 4C088 AB64 AC05 BA09 CA05 CA08 CA14 MA07 MA21 MA28 MA63 ZA89

Claims (3)

[Claims] The present invention is characterized in that a water or ethanol solution extract of olive leaves is treated by a column chromatography method using a synthetic polymer or bonded silica gel filler, and the obtained fraction is used as an essential component. Skin external preparation composition. 2. The composition for external use on skin according to claim 1, wherein the eluate in the column chromatography method has an ethanol concentration of 30 to 60% by volume. 3. The composition for external use on skin according to claim 1, wherein the composition for external use on skin is a cosmetic having an active oxygen scavenging action.