JP2000044485A - Active oxygen species scavenger and skin cosmetic - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain an active oxygen species scavenger with high safety and high activity to scavenge active oxygen species involved in disease aggravation and skin aging. SOLUTION: This active oxygen species scavenger is obtained by subjecting red kernel rice and/or bran afforded by polishing it to extraction with a hydrophilic organic solvent such as methyl alcohol, ethyl alcohol, glycerol, propylene glycol, 1, 3-butylene glycol, dipropylene glycol or isoprene glycol, or its mixture with water.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a reactive oxygen species scavenger and a skin cosmetic. More specifically, it relates to a reactive oxygen species scavenger that helps maintain homeostasis by eliminating excess in vivo reactive oxygen species that are deeply involved in the exacerbation of disease. The present invention relates to a skin cosmetic having an excellent effect of preventing or improving skin aging such as rough skin, wrinkles, stains, blemishes and abnormal deposition of pigments, and also having excellent safety.

[0002]

2. Description of the Related Art In recent years, active oxygen species closely related to aging have been attracting much attention. The reactive oxygen species means oxygen species activated from ordinary oxygen, and particularly those related to living organisms such as superoxide (O ₂ ⁻ ), hydrogen peroxide (H ₂ O ₂ ), and hydroxyl radical. (HO.) And singlet oxygen ( ¹ O ₂ ).

When bacteria, viruses, foreign substances, and the like enter the living body, leukocyte phagocytes are activated, and as a result, various reactive oxygen species are produced, attacking bacteria, viruses, foreign substances, and the like, thereby damaging the living body. Defend against such external enemies.

However, in recent years, it has become clear that excessive existence of reactive oxygen species in a living body causes various obstacles. Superoxide, which is produced in the body and also serves as a starting material for other reactive oxygen species, is sequentially digested by superoxide dismutase (SOD), which is a scavenging enzyme. When the activity of HO is decreased, the elimination of reactive oxygen species becomes insufficient, and the production of more troublesome H ₂ O ₂ and HO. Becomes excessive, which causes tissue damage such as rheumatoid arthritis and Behcet's disease. It is known to cause diseases such as myocardial infarction, stroke, and cataract.

[0005] On the other hand, the skin is directly affected by environmental factors such as ultraviolet rays, and is therefore an organ that easily produces reactive oxygen species. In combination with the increase in the concentration of reactive oxygen species and the production of lipid peroxide associated therewith, various factors are involved. Easy to cause serious obstacles.

For example, it is known that inflamed skin or skin exposed to ultraviolet rays generates reactive oxygen species. To avoid oxidative damage to living organisms caused by the reactive oxygen species, SOD, catalase, Attempts to maintain homeostasis by the action of scavenging enzymes such as glutathione peroxidase.

However, if excess reactive oxygen species remain in the skin without erasure, they act on melanocytes, which are pigment cells, to promote abnormal production of melanin, promote pigmentation, and to normalize skin tissues. It promotes the cross-linking and fragmentation of elastin and collagen, which are components of the extracellular matrix that exists to maintain the shape, and reduces the elasticity and wrinkles of the skin. Excessive reactive oxygen species are known to oxidize squalene, a skin lipid, and cell lipid membranes, thereby reducing the function of cells and exacerbating spots, wrinkles, and rough skin.

Therefore, in order to prevent and improve the skin aging effect caused by these reactive oxygen species, research on components for eliminating reactive oxygen species has been advanced, and the above-mentioned SOD which is a biological component has been studied.
And glutathione, vitamin C, vitamin E, vitamin B
Synthetic compounds such as 2, 2-butylhydroxytoluene, and butylhydroxyanisole have been found, but all have poor stability and safety, and more useful compounds have been demanded.

In recent years, attempts have been made to search for active oxygen species-eliminating components from plants, which are natural products, with an emphasis on safety.
Ellagic acid such as eucalyptus, flavonoids of cereals such as barley, black rice and black beans, catechin of tea, sesamin of sesame,
Carnosol and rosemanol, etc., found in herbs such as sage and rosemary, have been found, but all have poor content, solubility in extraction solvents, and stability, and their effects are not sufficient, and they are not practical. Was not satisfactory.

Attempts have also been made to find active oxygen species-eliminating components from rice and rice bran, which are normally eaten by humans and whose safety has been confirmed. Cinnamic acid derivatives extracted from wild rice are active oxygen species-eliminating. It has been found to have an effect (see JP-A-8-26983).

However, the content of these cinnamic acid derivatives is extremely low, and there is almost no commercial cropping of wild rice, so that it is very difficult to put them to practical use.

On the other hand, a study has been made on a hot water extract of abundant white rice bran (Japanese Patent Laid-Open No. 8-15132).
No. 0), when the bran is extracted with hot water, the extract becomes porridge and not only is extremely difficult to separate the bran and the extract, but also the antioxidant action of the obtained components has been confirmed. However, the effect of eliminating reactive oxygen species has hardly been confirmed.

[0013]

DISCLOSURE OF THE INVENTION The present invention has been made in view of the above problems, and has as its object to provide an excellent safety and an effect of eliminating active oxygen species involved in exacerbation of diseases and aging of skin. An object of the present invention is to provide an excellent active oxygen species eliminating agent.

In addition, the present invention eliminates active oxygen species of the skin caused by irradiation with ultraviolet rays or the like by blending the above active oxygen species scavenger as an active ingredient, thereby eliminating wrinkles, wrinkles, spots, rough skin, and elasticity of the skin. It is an object of the present invention to provide a skin cosmetic having an excellent effect of preventing and improving skin aging such as reduction of skin color and abnormal deposition of pigment.

[0015]

Means for Solving the Problems The present inventors have conducted intensive studies to solve the above-mentioned problems, and as a result, have found that a red organic brown rice or a bran obtained by polishing red rice brown rice is converted into a hydrophilic organic solvent or hydrophilic rice. The present inventors have found that an extract obtained by using a mixed solvent of a water-soluble organic solvent and water has an extremely high elimination action of active oxygen species, and have completed the present invention.

The red rice used in the present invention is a kind of rice that is native to tropical Asia, has a unique red color, is also called the roots of red rice, has been imported into Japan through China and other countries, and has been eaten to date. Is known to be. Red rice is derived from ordinary white rice and its origin, Oryza Sativa.
Although common as Sativa), it is characterized by a large amount of red pigment in the peel and seed coat of brown rice, which is different from white rice in this point.

As a raw material for obtaining the active oxygen species scavenger of the present invention, the above-described red rice brown rice or bran obtained by polishing brown rice is used, either alone or in combination with red rice brown rice and its bran. May be used as appropriate, and in view of the activity and economical efficiency of the obtained active oxygen species scavenger, it is efficient to use red rice bran obtained by milling rice.

When brown rice is used as an extraction raw material, it may be used as it is, but it is preferable to carry out crushing in order to increase the extraction efficiency. On the other hand, when red rice bran is used, it can be used as an extraction raw material as it is.

The active oxygen species-eliminating agent of the present invention can be extracted by adding an appropriate extraction solvent to the extraction raw material in the same manner as when using these as the extraction raw material to obtain an extract such as a usual extractant or impregnating agent. . As the solvent that can be used to extract the active oxygen species-eliminating agent from these extraction raw materials, hydrophilic organic solvents, that is, various organic solvents that can be mixed with water, for example, methyl alcohol, ethyl Alcohol, glycerin, 1,3-butylene glycol, propylene glycol, dipropylene glycol,
Examples thereof include isobutylene glycol and a hydrophilic organic solvent thereof. Further, two or more of these hydrophilic organic solvents may be appropriately mixed, or one or two or more of these hydrophilic organic solvents and water may be appropriately mixed and used. At this time, the mixing ratio of the hydrophilic organic solvent and water can be arbitrarily set, but is preferably 7: 3 by weight.
It is preferable to use a mixed solvent with water rather than a hydrophilic organic solvent alone.

In extracting with these solvents,
It is convenient to previously degrease the extraction raw material using a nonpolar solvent such as hexane, so that the active ingredient can be extracted more efficiently.

The extraction is carried out by adding an extraction solvent in a weight ratio of about 5 to 15 times with respect to red rice brown rice and / or bran, immersing the mixture, and gently loosening the mixture at room temperature to about 100 ° C. for several to several tens of hours. While stirring and mixing, the soluble components are sufficiently eluted. Thereafter, solid-liquid separation is performed using a known solid-liquid separation method such as filtration with a filter paper or filter cloth, filter press, or centrifugation to obtain an extract containing an active oxygen species-eliminating component.

The obtained extract can be used as it is as an active oxygen species eliminating agent of the present invention. However, the solvent can be distilled off to obtain a paste or a dried solid. . In addition, the extract can be subjected to simple deodorizing and decoloring treatment with activated carbon or the like to the extent that active ingredients are not lost. Further, so-called excipients such as various starches and lactose are appropriately added to such an extent that the active oxygen species elimination effect is not lost so as to be easily used according to the purpose of use and the method of use as described below, and the activity of the present invention is improved. An oxygen species scavenger can also be used.

Although the active component (essence) of the active oxygen species scavenger of the present invention thus obtained is not clear, columns such as Diaion CHP-20 and Sephadex LH-20 which are synthetic polymer adsorbents are used. The active fraction obtained by separation and fractionation by chromatography is analyzed by nuclear magnetic resonance (N
As a result of confirming by MR) spectral method, active ingredient (fraction)
In some of them, proanthocyanidins, which are polymers having flavan as a basic skeleton, could be confirmed, but cinnamic acid derivatives could not be confirmed.

The active oxygen species scavenger of the present invention is made from red rice, which has been used as a staple food since ancient times, as described above, and its safety has been confirmed. In addition, since off-flavors and off-flavors are extremely weak, they can be used in general foods and drinks, for example, soft drinks, tea drinks, dairy products, processed vegetable and fruit products, confectionery, etc., and are usually used in producing these. Raw materials and additives, such as sugars such as glucose, fructose, and sucrose; organic acids such as citric acid and malic acid; vitamin B
Group, vitamin C (L-ascorbic acid), vitamins such as vitamin E, polyhydric alcohols such as glycerin, propylene glycol and sorbitol, surfactants such as glycerin fatty acid ester and sucrose fatty acid, other carrageenan, casein and gelatin , Pectin, agar, amino acids, calcium salts, pigments, flavors, preservatives and the like.

Further, the active oxygen species scavenger of the present invention can be used as health foods and pharmaceuticals, and can be added to cosmetics and the like to prevent / improve various obstacles caused by the excessively present in vivo active oxygen species. It can be widely used.

The preferred amount of use varies depending on the object to be added, but in the case of foods and health foods, the active oxygen species scavenger of the present invention is used as an extract and dried product in an amount of from 0.01 to 0.01%.
20% by weight.

Furthermore, the active oxygen species-eliminating agent of the present invention is absorbed transdermally and exhibits an effect. Therefore, in order to utilize it most effectively, it is to be incorporated into a skin cosmetic. Suitable skin cosmetics to be compounded include, for example, ointments, creams, emulsions, lotions, packs, bath preparations and the like. In addition, these skin cosmetics contain the active oxygen species-eliminating agent of the present invention as an extracted and dried product in an amount of 0.00
The effect can be obtained by mixing 1 to 10% by weight.

The skin cosmetic according to the present invention, when applied to the skin, quickly eliminates excess reactive oxygen species produced by external stimuli such as ultraviolet rays, and aging of the skin, especially wrinkles, spots, tarmi and the like. It can prevent and improve blemishes, rough skin, abnormal pigment deposition, and the like.

The skin cosmetics according to the present invention can use the same raw materials as ordinary skin cosmetics. Examples of the raw materials include oily components such as avocado oil, rice bran oil, rice germ oil, lanolin, squalane, and the like. Glycerin, 1,3-
Moisturizers such as butylene glycol, collagen, hyaluronic acid and its salts, chondroitin sulfate and its salts, chitosan, chitin, etc., complex lipids such as glycerophospholipid, sphingolipid, and glyceroglycolipid, SOD, catalase, β-carotene, and Ichiyo extract Substances, vitamin C and its derivatives, vitamin E and its derivatives, gongo extract, kujin extract and other active oxygen species-eliminating active ingredients, guaiazulene and its derivatives, kamaazulene and its derivatives, glycyrrhizic acid and its salts, and glycyrrhetinic acid and its Salts, glycyrrhetinic acid derivatives, anti-inflammatory agents such as zinc oxide, and other various plant extracts, thickeners, preservatives, ultraviolet absorbers, fragrances, antioxidants, water, various alcohols, and the like.

[0030]

DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in more detail based on embodiments. (Example 1) 30 kg to 10 kg of crushed red rice brown rice
A 100% by weight aqueous ethanol solution was added, heated, and extracted under reflux cooling for 5 hours. The obtained extract is filtered through filter paper, the filtrate is concentrated under reduced pressure, and the remaining paste is freeze-dried to obtain a dried product 28 which is the active oxygen species scavenger of the present invention.
0 g was obtained.

Example 2 Bran 1 obtained by milling red rice brown rice
To 0 kg, 100 l of n-hexane was added, and the mixture was slowly stirred for 2 hours while heating to 50 ° C. to extract the oil and fat of bran, followed by solid-liquid separation by filter paper filtration. Thereafter, 100 l of n-hexane was added to the residue, and the same operation as above was performed.
Further, fats and oils were removed, and the solvent was distilled off from the obtained residue under reduced pressure. 100 l of a 90% by weight aqueous ethanol solution is added to the obtained residue, and the mixture is stirred at room temperature with gentle stirring.
Extraction was performed for 0 hours, and an extract was obtained by filtration through a filter paper. The extract was concentrated under reduced pressure, and the remaining paste was freeze-dried to obtain 730 g of a dried product that was the active oxygen species eliminating agent of the present invention.

Example 3 To a total amount of defatted red rice bran obtained in the same manner as in Example 2, 70 l of an aqueous solution of 50% by weight of 1,3-butylene glycol was added, and the mixture was slowly stirred under heating at 95 ° C. Extraction was performed for 5 hours, and filtration with filter paper was performed. Thereafter, the extract was allowed to stand at 5 ° C. for 5 days, and the generated cages and precipitates were removed by filtration using diatomaceous earth to obtain 43 l of a clear extract which was the active oxygen species eliminating agent of the present invention. In addition,
The obtained extract was dried under reduced pressure, and the solid content in the extract was measured. The result was 1.06% by weight.

[Effect of elimination of active oxygen species] Next, the elimination of active oxygen species of the dried products obtained in Examples 1 and 2 will be described.
Tests were performed on the SOD-like activity (NBT method), the lipid membrane peroxidation inhibitory action by singlet oxygen, the hydrogen peroxide scavenging action, and the radical scavenging action on DPPH by the following methods. In each test, the above extract was dissolved or suspended in water or PBS so as to have an appropriate concentration, and the test was performed using sample solutions of several concentrations having different concentrations. Alternatively, an action concentration at which the inhibition rate was 50% was determined. As a comparative example, an extract and dried product obtained by the same operation as in Example 2 was used for bran obtained by polishing white rice (Koshihikari) brown rice.

1. SOD-like activity test (NBT method) A 0.05 M Na ₂ CO ₃ buffer solution (pH 10.
2) 2.4 ml, 3 mM Xanthine 0.1 m
Then, 0.1 ml of 3 mM EDTA (sodium ethylenediaminetetraacetate), 0.1 ml of BSA (bovine serum albumin) solution and 0.1 ml of 0.75 mM NBT (nitro blue tetrazolium) were added thereto, and 0.1 ml of the sample solution was added.
Add 1 ml and leave at 25 ° C. for 10 minutes. Then X
Add 0.1 ml of anthine oxidase solution, stir quickly, and incubate at 25 ° C for 20 minutes. Thereafter, 0.1 ml of 6 mM CuCl ₂ is added to stop the reaction, and the absorbance St at 560 nm is measured. As a measurement control, the same operation is performed using distilled water instead of the sample solution, and the absorbance Bt is measured. In addition, as a sample solution blank and a control blank, the same operation was performed using distilled water instead of the Xanthine Oxidase solution, and the absorbance So and the absorbance Bo were measured, and the SOD-like activity inhibition rate was calculated from the following equation 1. I do. Suppression rate (%) = [1-{(St-So) / (Bt-Bo)}] × 100 (Equation 1) The Xanthine Oxidase solution is Xa
nthine Oxidase (manufactured by Boehringer) is diluted about 100-fold with distilled water, and the same operation as above is performed so that the absorbance Bt of the measurement control falls within the range of 0.20 to 0.23.

2. Test for inhibition of membrane lipid peroxidation by singlet oxygen (erythrocyte hemolysis test) 2% RBC in a clear glass 10 ml sample bottle
5 ml of the suspension, 5 ml of a PBS solution (pH 7.0 isotonic phosphate buffer) containing the sample at each concentration and 0.01 ml of a photosensitizer (10 mM hematoporphyrin-20 mM sodium hydroxide solution) were added. Mix. The reaction solution is uniformly irradiated with light on a merry-go-round for 35 minutes with a 7.5 W halogen lamp to generate singlet oxygen and lyse red blood cells. 1 ml of this reaction solution is collected and PB
Add 2 ml of S and stir well.
After centrifugation at pm for 5 minutes, the supernatant
Measure absorbance SA in nm. On the other hand, as a control solution, a completely hemolyzed reaction solution was obtained by the same operation using 5 ml of a PBS solution to which no sample was added.
ml and operating at 540
The absorbance SB in nm is measured, and the peroxidation inhibition rate is calculated by the following equation 2. Peroxidation inhibition rate (%) = {(SB-SA) / SB} × 100 (Formula 2) The RBC suspension used was obtained by the following method. Heparin blood obtained by collecting blood from rabbits 10 to 20 m
1 is placed in a 50 ml Erlenmeyer flask containing 5 glass beads and gently rotated for 10 minutes. Leave for several minutes to obtain defibrinated blood with fibrin deposited. This is added at 4 ° C and 3000 rpm for 1 hour.
After centrifugation for 0 minutes to remove the supernatant, PBS 3 ml
And centrifuged at 3000 rpm for 10 minutes at 4 ° C.
Washing is repeated enough (about 3 times), and washed RBC
(Red blood cells). A predetermined amount of PBS is added to the RBC to make a 2% RBC suspension.

3. Hydrogen peroxide elimination test 10 μl of a sample solution is added to 10 μl of a standard solution (1.5 mM) of hydrogen peroxide, and the mixture is incubated at 37 ° C. for 20 minutes. Then, 2.98 ml of a coloring solution is added, and the mixture is incubated at 37 ° C. for 5 minutes. The absorbance St at 727 nm of the solution is measured. As a comparative control, the same operation was performed using distilled water instead of the sample solution to obtain an absorbance S
Measure b. At this time, as a blank, the absorbances Sto and Sbo at 727 nm immediately after the coloring solution was added.
Is measured, and the elimination rate of hydrogen peroxide is measured by the following equation (3). Erasure rate (%) = [1-{(St-Sto) / (Sb-Sbo)}] × 100 (Equation 3) The coloring solution is DA-64 (N- (carboxymethylaminocarbonyl) 100 μM of -4,4′-bis (dimethylamino) -diphenylaminosodium (manufactured by Wako Pure Chemical Industries) and 0.5 w / v of triton X-100.
% MPIPES (piperazine-N, N'-
Bis (2-ethanesulfonic acid)) buffer (pH 7.0)
1 mg of peroxidase (100 units / mg: manufactured by Wako Pure Chemical Industries, Ltd.) was added and dissolved to make 100 ml.

4. Radical scavenging test for DPPH 1.5 × 10 ^-4 MDPPH (α, α-diphenyl-β-
3 ml of a sample solution is added to 3 ml of an ethanol solution of (picrylhydrazyl), sealed, shaken, and left to stand for 30 minutes. After standing, the absorbance St at 520 nm is measured. In addition, as a control test, the same operation is performed using distilled water instead of the sample solution, and the absorbance Sb at 520 nm is measured. Also, as a blank, immediately after adding 3 ml of the sample solution to 3 ml of the ethanol solution of DPPH, the absorbance So at 520 nm is measured, and the radical scavenging rate is calculated by the following equation 4. Radical scavenging rate = [{Sb− (St−So)} / Sb] × 100 (Equation 4)

[Test Results] The results of the above tests are summarized in Table 1. As can be seen from Table 1, it was confirmed that the reactive oxygen species were eliminated in the present invention, and it was confirmed that the active oxygen species eliminating effect of the extract of white rice bran was extremely higher.

[0039]

[Table 1]

Next, various skin cosmetics were prepared using the active oxygen species eliminating agent obtained in each of the above examples, and a use test was conducted on the emulsion of the skin cosmetics. (Formulation Example 1) Emulsion component Content (% by weight) Active oxygen species scavenger of Example 2 1.0 1,3-butylene glycol 5.0 Carboxyvinyl polymer 0.13 Jojoba oil 3.0 Squalane 2.0 Methylphenyl polysiloxane 3.0 Polyoxyethylene cetyl ether (1EO) 1.2 Sucrose fatty acid ester (HLB4) 0.5 Polyoxyethylene hydrogenated castor oil (50EO) 1.5 Tocopherol 0.15 Paraoxybenzoate 0.2 L-arginine 0.13 Purified water qs (Production method) Among the above components, eight components from jojoba oil to paraoxybenzoate are heated and uniformly dissolved to form an oil phase. . The remaining components are heated to be uniformly dissolved to form an aqueous phase, and the oil phase is added to the aqueous phase with stirring to emulsify uniformly, and then gradually cooled to obtain an emulsion as the skin cosmetic of the present invention.

(Formulation Example 2) Cream component Blending amount (% by weight) Active oxygen species scavenger of Example 1 4.0 Glycerin 5.0 Cetyl alcohol 4.0 Stearic acid 3.0 Squalane 10.0 Lipophilic type Glyceryl monostearate 2.0 Sorbitan sesquioleate 1.0 Polyoxyethylene hydrogenated castor oil (50 EO) 1.5 Tocopherol 0.15 Carboxyvinyl polymer 0.1 Potassium hydroxide 0.05 Paraoxybenzoate 0 2.2 Appropriate amount of purified water (Production method) Seven components from cetyl alcohol to tocopherol are heated and uniformly dissolved to form an oil phase. The remaining components are heated to be uniformly dissolved to form an aqueous phase, and the oil phase is added to the aqueous phase with stirring to emulsify uniformly, and then gradually cooled to obtain the cream, which is the skin cosmetic of the present invention.

(Formulation Example 3) Lotion lotion component content (% by weight) Active oxygen species scavenger of Example 3 6.0 glycerin 3.0 1,3-butylene glycol 4.0 monolauric acid polyoxyethylene sorbitan ( 20EO) 0.5 Citric anhydride 0.01 Paraoxybenzoate 0.1 Purified water Appropriate amount (Production method) Take the above-mentioned components and dissolve them uniformly to obtain a lotion as the skin cosmetic of the present invention. obtain.

(Formulation Example 4) Packing agent Component Compounding amount (% by weight) Active oxygen species scavenger of Example 2 2.0 Stearic acid 5.0 Cetyl alcohol 4.0 Sorbitan sesquioleate 1.0 Monolauric acid Poly Oxyethylene sorbitan (20EO) 2.5 Tocopherol 0.15 Self-emulsifying glyceryl monostearate 4.0 Squalane 15.0 Kaolin 5.0 Talc 5.0 1,3-butylene glycol 5.0 Glycerin 3.0. 0 Xanthan gum 0.2 Paraoxybenzoate 0.2 Purified water Adequate amount (Production method) The seven components from stearic acid to squalane are heated and uniformly dissolved to form an oil phase. The remaining components are heated and uniformly dissolved to form an aqueous phase.After stirring, the oil phase is added to the aqueous phase and uniformly emulsified, and then gradually cooled, and then kaolin and talc are added and uniformly mixed. A pack which is the skin cosmetic of the present invention is obtained.

(Formulation Example 5) Bath Agent Ingredient Compounding Amount (% by Weight) Active Oxygen Species Elimination Agent of Example 1 1.0 Oleyl Alcohol 10.0 Liquid Paraffin 45.0 Squalane 5.0 Sorbitan Sesquioleate 6. 0 Polyoxyethylene hydrogenated castor oil (20 EO) 2.0 1,3-butylene glycol 5.0 Paraoxybenzoic acid ester 0.05 Fragrance 1.0 Purified water Appropriate amount (Production method) Oleyl alcohol or less polyoxy Heat and uniformly dissolve the five components up to ethylene-hardened castor oil. Next, the remaining components are heated and uniformly dissolved to form an aqueous phase, and the aqueous phase is added to the oil phase while stirring to emulsify uniformly,
Thereafter, the emulsion is cooled to obtain an emulsion bath agent which is the skin cosmetic of the present invention.

[Evaluation Test] Next, 20 women (23-38 years of age) who exhibited rough skin with respect to the emulsion of Formulation Example 1 described above.
Were actually used by the panelists, and the following evaluation test was performed. As a comparative control, an emulsion prepared by removing the active oxygen species eliminating agent from the above formulation was used.

1. Skin roughness improvement effect test For women aged 18 to 40 years, the surface skin morphology of the face was determined by Sylflo (Flexicl Developments).
A replica of the skin was taken using a replica method (Ltd. Ltd.), and observed with a microscope of 50 times magnification. Based on the evaluation criteria shown below, evaluation 1 or 2 was performed based on the skin crest state and the exfoliation state. Twenty women determined to have rough skin corresponding to were selected as panelists. The emulsion of Formulation Example 1 and the emulsion of Comparative Example were applied to the left and right faces of the face of these 20 panelists once each morning and evening for 30 days. After 30 days, the skin condition was observed again using the replica method, and the degree of improvement was evaluated according to the same evaluation criteria.

(Evaluation Criteria) Evaluation Evaluation Contents Remarks 1 The skin sulcus and skin hills have disappeared. Rough skin The horny layer is turned up over a wide area. 2 Skin sulcus and skin ridge are unclear. Rough skin Partially turned up in the stratum corneum. 3 Skin sulcus and skin ridge are observed, but are flat. Normal skin 4 Skin grooves and skin hills are clear. Relatively clean skin 5 Skin grooves and skin ridges are extremely clear and well-organized. Beautiful skin

2. Miczameter MX-16 (manufactured by Eurotech Japan) was used for the 20 panelists selected for the skin roughness improvement effect test before and after applying each emulsion for 30 days each morning and evening. The amount of melanin and erythema in the skin was measured using the samples, and the change in the values was used to evaluate the effect of improving skin color. The evaluation method is the ratio of the measured values before and after use of each emulsion [(measured value after use / measured value before use) × 100]
(%) Was calculated, and the degree of improvement was evaluated based on the following evaluation criteria.

(Evaluation Criteria) Evaluation Evaluation Contents Remarks 1 Remarkable improvement of both melanin and erythma. 10% or more reduction. 2 Improvement of both melanin and erythma. 5-10% reduction. 3 Melanin. There is a slight improvement in both the amount of erythema and the amount of erythema 1-5% decrease 4 There is no improvement effect with the amount of melanin and erythma 1% or less decrease

3. Evaluation test of use feeling and use effect by monitor A questionnaire survey was conducted on the following items about the use feeling and the effect on the skin after use for 20 panelists selected in the above-mentioned skin roughness improvement effect test.

[Test Results] The test results of the above-described skin roughness improvement effect test, blemishes improvement effect test, and evaluation tests of the feeling of use and the effect of use are shown below.

(Roughness Improvement Effect Test) Evaluation Skin Before Application Skin After Application Example Skin After Application Comparative Example 18 (N) 0 (N) 6 (N) 2 12 0 8 3 0 5 4 4 0 12 25 0 30

(Kusumi improvement effect test) Evaluation Example application Comparative example application 13 (name) 0 (name) 2 9 1 3 6 11 4 2 8

(Use feeling, use effect test) Item Number of people who made the example better Number of people who made the comparative example better ・ Familiarity to skin 13 (N) 6 (Person) ・ Moist feeling 19 1 ・ Stretch to skin 13 5 -180 satisfaction for improvement of rough skin-134 satisfaction for improvement of skin color-Number and depth of wrinkles 16 2 Improvement effect For items that did not add up to 20 persons, they answered that they were about the same.

As can be seen from the above results, the skin cosmetic of the present invention has a remarkable improvement effect on rough skin and dark spots, and the actual feeling of use is superior to that of the comparative example. It was confirmed that there was.

Needless to say, the present invention is not limited to the above Examples, and the activity of the present invention can be improved by using various hydrophilic organic solvents from red rice brown rice and / or bran obtained by polishing red rice brown rice. It is needless to say that an oxygen species scavenger can be obtained, and the active oxygen species scavenger can be used in a wide range of foods and skin cosmetics.

[0057]

According to the present invention, it is possible to provide an entirely new active oxygen species scavenger which has extremely high active oxygen species scavenging activity and high safety. As a result, it is possible to provide a skin cosmetic or the like having an excellent effect of preventing or improving skin aging such as rough skin, wrinkles, spots, dark spots and abnormal deposition of pigments due to external stimuli such as sunburn.

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Claims (3)

[Claims] The present invention is characterized in that it comprises a hydrophilic organic solvent or an extract obtained by using a mixed solvent of these with water from red rice brown rice and / or bran obtained by polishing red rice brown rice. Reactive oxygen species scavenger. 2. The active oxygen species scavenger according to claim 1, wherein the hydrophilic organic solvent is methyl alcohol, ethyl alcohol, glycerin, propylene glycol, 1,3-butylene glycol, dipropylene glycol, or isoprene glycol. 3. A skin cosmetic comprising the active oxygen species scavenger according to claim 1 or 2.