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HUP9900603A1 - Eljárás és berendezés magasabbrendű növények polarizálható sejtjeinek immobilizálására nagy frekvenciájú váltóáram alkalmazásával - Google Patents

Eljárás és berendezés magasabbrendű növények polarizálható sejtjeinek immobilizálására nagy frekvenciájú váltóáram alkalmazásával

Info

Publication number
HUP9900603A1
HUP9900603A1 HU9900603A HUP9900603A HUP9900603A1 HU P9900603 A1 HUP9900603 A1 HU P9900603A1 HU 9900603 A HU9900603 A HU 9900603A HU P9900603 A HUP9900603 A HU P9900603A HU P9900603 A1 HUP9900603 A1 HU P9900603A1
Authority
HU
Hungary
Prior art keywords
higher plants
immobilization
high frequency
alternate current
cells
Prior art date
Application number
HU9900603A
Other languages
English (en)
Inventor
Beáta Barnabás
Zsolt Pónya
Péter Finy
Original Assignee
MTA Mezőgazdasági Kutatóintézete
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by MTA Mezőgazdasági Kutatóintézete filed Critical MTA Mezőgazdasági Kutatóintézete
Priority to HU9900603A priority Critical patent/HUP9900603A1/hu
Publication of HU9900603D0 publication Critical patent/HU9900603D0/hu
Priority to PCT/HU2000/000021 priority patent/WO2000055339A2/en
Priority to AU34480/00A priority patent/AU3448000A/en
Publication of HUP9900603A1 publication Critical patent/HUP9900603A1/hu

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

A találmány tárgya eljárás és berendezés magasabb rendű növényekpolarizálható sejtjeinek immobilizálására nagy frekvenciájú váltóáramalkalmazásával. A találmány tárgyát képezik a találmány szerintieljárás és berendezés alkalmazásával végrehajtott mikroinjektálásieljárások is, melyek alkalmasak exogén DNS bejuttatására különbözőnövényi sejtekbe, például búzapetesejtekbe/zigótákba. Ó
HU9900603A 1999-03-12 1999-03-12 Eljárás és berendezés magasabbrendű növények polarizálható sejtjeinek immobilizálására nagy frekvenciájú váltóáram alkalmazásával HUP9900603A1 (hu)

Priority Applications (3)

Application Number Priority Date Filing Date Title
HU9900603A HUP9900603A1 (hu) 1999-03-12 1999-03-12 Eljárás és berendezés magasabbrendű növények polarizálható sejtjeinek immobilizálására nagy frekvenciájú váltóáram alkalmazásával
PCT/HU2000/000021 WO2000055339A2 (en) 1999-03-12 2000-03-13 Method and device for immobilization of polarizable cells of higher plants by the use of a high frequency ac-field
AU34480/00A AU3448000A (en) 1999-03-12 2000-03-13 Method and device for immobilization of polarizable cells of higher plants by the use of a high frequency ac-field

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
HU9900603A HUP9900603A1 (hu) 1999-03-12 1999-03-12 Eljárás és berendezés magasabbrendű növények polarizálható sejtjeinek immobilizálására nagy frekvenciájú váltóáram alkalmazásával

Publications (2)

Publication Number Publication Date
HU9900603D0 HU9900603D0 (en) 1999-05-28
HUP9900603A1 true HUP9900603A1 (hu) 2000-12-28

Family

ID=89997928

Family Applications (1)

Application Number Title Priority Date Filing Date
HU9900603A HUP9900603A1 (hu) 1999-03-12 1999-03-12 Eljárás és berendezés magasabbrendű növények polarizálható sejtjeinek immobilizálására nagy frekvenciájú váltóáram alkalmazásával

Country Status (3)

Country Link
AU (1) AU3448000A (hu)
HU (1) HUP9900603A1 (hu)
WO (1) WO2000055339A2 (hu)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7014744B2 (en) * 2001-08-24 2006-03-21 Applera Corporation Method of purification and concentration using AC fields with a transfer tip
FR2969497B1 (fr) * 2010-12-27 2013-06-28 Ceva Sante Animale Composition luminescente comme biomarqueur dans un oeuf aviaire, dispositif et procede correspondants.

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4970154A (en) * 1987-10-09 1990-11-13 Baylor College Of Medicine Method for inserting foreign genes into cells using pulsed radiofrequency
US5714359A (en) * 1995-10-12 1998-02-03 The University Of Akron Apparatus and method for electrostatic endothelial cell seeding in a vascular prosthesis
WO1998001575A1 (en) * 1996-07-08 1998-01-15 Pioneer Hi-Bred International, Inc. Transformation of zygote, egg or sperm cells and recovery of transformed plants from isolated embryo sacs

Also Published As

Publication number Publication date
AU3448000A (en) 2000-10-04
WO2000055339A3 (en) 2000-12-28
HU9900603D0 (en) 1999-05-28
WO2000055339A2 (en) 2000-09-21

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