[go: up one dir, main page]

HUP9900603A1 - Method and apparatus for immobilization of polarizable cells of higher plants using high frequency alternate current - Google Patents

Method and apparatus for immobilization of polarizable cells of higher plants using high frequency alternate current

Info

Publication number
HUP9900603A1
HUP9900603A1 HU9900603A HUP9900603A HUP9900603A1 HU P9900603 A1 HUP9900603 A1 HU P9900603A1 HU 9900603 A HU9900603 A HU 9900603A HU P9900603 A HUP9900603 A HU P9900603A HU P9900603 A1 HUP9900603 A1 HU P9900603A1
Authority
HU
Hungary
Prior art keywords
higher plants
immobilization
high frequency
alternate current
cells
Prior art date
Application number
HU9900603A
Other languages
Hungarian (hu)
Inventor
Beáta Barnabás
Zsolt Pónya
Péter Finy
Original Assignee
MTA Mezőgazdasági Kutatóintézete
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by MTA Mezőgazdasági Kutatóintézete filed Critical MTA Mezőgazdasági Kutatóintézete
Priority to HU9900603A priority Critical patent/HUP9900603A1/en
Publication of HU9900603D0 publication Critical patent/HU9900603D0/en
Priority to PCT/HU2000/000021 priority patent/WO2000055339A2/en
Priority to AU34480/00A priority patent/AU3448000A/en
Publication of HUP9900603A1 publication Critical patent/HUP9900603A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

A találmány tárgya eljárás és berendezés magasabb rendű növényekpolarizálható sejtjeinek immobilizálására nagy frekvenciájú váltóáramalkalmazásával. A találmány tárgyát képezik a találmány szerintieljárás és berendezés alkalmazásával végrehajtott mikroinjektálásieljárások is, melyek alkalmasak exogén DNS bejuttatására különbözőnövényi sejtekbe, például búzapetesejtekbe/zigótákba. ÓThe subject of the invention is a method and apparatus for immobilizing polarizable cells of higher plants using high-frequency alternating current. The subject of the invention are also microinjection procedures performed using the method and equipment according to the invention, which are suitable for introducing exogenous DNA into various plant cells, for example wheat ovules/zygotes. HE

HU9900603A 1999-03-12 1999-03-12 Method and apparatus for immobilization of polarizable cells of higher plants using high frequency alternate current HUP9900603A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
HU9900603A HUP9900603A1 (en) 1999-03-12 1999-03-12 Method and apparatus for immobilization of polarizable cells of higher plants using high frequency alternate current
PCT/HU2000/000021 WO2000055339A2 (en) 1999-03-12 2000-03-13 Method and device for immobilization of polarizable cells of higher plants by the use of a high frequency ac-field
AU34480/00A AU3448000A (en) 1999-03-12 2000-03-13 Method and device for immobilization of polarizable cells of higher plants by the use of a high frequency ac-field

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
HU9900603A HUP9900603A1 (en) 1999-03-12 1999-03-12 Method and apparatus for immobilization of polarizable cells of higher plants using high frequency alternate current

Publications (2)

Publication Number Publication Date
HU9900603D0 HU9900603D0 (en) 1999-05-28
HUP9900603A1 true HUP9900603A1 (en) 2000-12-28

Family

ID=89997928

Family Applications (1)

Application Number Title Priority Date Filing Date
HU9900603A HUP9900603A1 (en) 1999-03-12 1999-03-12 Method and apparatus for immobilization of polarizable cells of higher plants using high frequency alternate current

Country Status (3)

Country Link
AU (1) AU3448000A (en)
HU (1) HUP9900603A1 (en)
WO (1) WO2000055339A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7014744B2 (en) * 2001-08-24 2006-03-21 Applera Corporation Method of purification and concentration using AC fields with a transfer tip
FR2969497B1 (en) * 2010-12-27 2013-06-28 Ceva Sante Animale LUMINESCENT COMPOSITION AS A BIOMARKER IN AN AVIAN EGG, CORRESPONDING DEVICE AND METHOD.

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4970154A (en) * 1987-10-09 1990-11-13 Baylor College Of Medicine Method for inserting foreign genes into cells using pulsed radiofrequency
US5714359A (en) * 1995-10-12 1998-02-03 The University Of Akron Apparatus and method for electrostatic endothelial cell seeding in a vascular prosthesis
WO1998001575A1 (en) * 1996-07-08 1998-01-15 Pioneer Hi-Bred International, Inc. Transformation of zygote, egg or sperm cells and recovery of transformed plants from isolated embryo sacs

Also Published As

Publication number Publication date
AU3448000A (en) 2000-10-04
WO2000055339A3 (en) 2000-12-28
HU9900603D0 (en) 1999-05-28
WO2000055339A2 (en) 2000-09-21

Similar Documents

Publication Publication Date Title
WO2003012058A3 (en) Media and methods for cultivation of microorganisms
MXPA02006145A (en) Method for isolation of rna from formalinfixed paraffinembedded tissue specimens.
DE122007000082I1 (en) METHOD FOR THE REPLICATION OF INFLUENZA IN CELL CULTURE AND THE INFLUENZA VIRUSES PRODUCED BY THIS PROCEDURE
DE60324213D1 (en) Apparatus and method for the production of electrically deionized water
ATE493001T1 (en) APPARATUS AND METHOD FOR TRANSFER BASED ON QUALITY OF SERVICE
WO2000070037A3 (en) Directed evolution of microorganisms
AU2003252396A8 (en) Apparatus for environment of cultivating/culturing plant, cultivation/culture method and cultivation/culture apparatus
ATE434975T1 (en) DEVICE AND METHOD FOR REPOSITIONING PATIENTS
EA200001129A3 (en) METHOD OF OBTAINING 4-CARBOXYAMINO-2-SUBSTITUTE-1,2,3,4-TETRAHYDROCHINOLINE
DE60039507D1 (en) PROCESS TRANSMITTER HAVING AN UPWARD TRANSFORMER FOR ENERGY SUPPLY OF ANALOG CONSTRUCTION ELEMENTS
AU2003238717A1 (en) POLYPEPTIDES HAVING Alpha- H-Alpha-AMINO ACID AMIDE RACEMASE ACTIVITY AND NUCLEIC ACIDS ENCODING THE SAME
IS2889B (en) Bv8 nucleic acids and polypeptides with mitosis-causing activity
HUP9900603A1 (en) Method and apparatus for immobilization of polarizable cells of higher plants using high frequency alternate current
AU2002361162A8 (en) Evanescence-based multiplex sequencing method
WO2003012029A3 (en) Method for selective enhancement of cell growth
DE60041340D1 (en) IDENTIFICATION OF PLASMODIUM FALCIPARUM ERYTHROZN TO CHONDROITIN SULFATE A MEDIATED
EE05162B1 (en) Method for the preparation of optically active amines
AU2159801A (en) Test for micro-organisms
AU6978900A (en) Method for producing triethylene diamine, using ethylene diamine
ATE417919T1 (en) MICROBIOLOGICAL PROCESS FOR PRODUCING AMIDES
ATE244706T1 (en) ENANTIOMERS OF MERCAPTOLACTONES AND METHOD FOR THE PRODUCTION THEREOF
WO2003035886A3 (en) Method for preparing heparin from mast cell cultures
DK1639119T3 (en) Process for microbiological isomerization of alpha-hydroxycarboxylic acids
DK1238057T3 (en) Process for producing adherent animal cells
DE3677217D1 (en) METHOD FOR REGENERATING CORN.