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GB911103A - Acid-stable proteolytic and amylolytic enzyme - Google Patents

Acid-stable proteolytic and amylolytic enzyme

Info

Publication number
GB911103A
GB911103A GB38299/59A GB3829959A GB911103A GB 911103 A GB911103 A GB 911103A GB 38299/59 A GB38299/59 A GB 38299/59A GB 3829959 A GB3829959 A GB 3829959A GB 911103 A GB911103 A GB 911103A
Authority
GB
United Kingdom
Prior art keywords
enzyme
acid
amylolytic enzyme
stable proteolytic
wheat bran
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB38299/59A
Inventor
Ichiro Tanaka
Takaaki Yanagisawa
Jiro Sawada
Yutaka Misawa
Hiroshi Nakayoshi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Taisho Pharmaceutical Co Ltd
Original Assignee
Taisho Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Taisho Pharmaceutical Co Ltd filed Critical Taisho Pharmaceutical Co Ltd
Priority to GB38299/59A priority Critical patent/GB911103A/en
Publication of GB911103A publication Critical patent/GB911103A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C12N9/242—Fungal source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/62—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

An acid stable proteolytic and amylolytic enzyme is obtained by cultivating Aspergillus oryzal var. microsporus TPR-18 on sterilised acidic wheat bran, extracting with water, adding zinc or cobalt chloride to stabilize the enzyme, concentrating the extract and then precipitating the enzyme by the addition of isopropanol. The wheat bran is treated with 20 to 50% by weight of 0.2 HCl and the cultivation is at 30 DEG C. for 40 to 50 hours. The zinc or cobalt chloride is used in quantities of 10-3 mol per litre of aqueous extract and then the pH is adjusted to 5 to 6 prior to concentration and precipitation.
GB38299/59A 1959-11-11 1959-11-11 Acid-stable proteolytic and amylolytic enzyme Expired GB911103A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB38299/59A GB911103A (en) 1959-11-11 1959-11-11 Acid-stable proteolytic and amylolytic enzyme

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB38299/59A GB911103A (en) 1959-11-11 1959-11-11 Acid-stable proteolytic and amylolytic enzyme

Publications (1)

Publication Number Publication Date
GB911103A true GB911103A (en) 1962-11-21

Family

ID=10402544

Family Applications (1)

Application Number Title Priority Date Filing Date
GB38299/59A Expired GB911103A (en) 1959-11-11 1959-11-11 Acid-stable proteolytic and amylolytic enzyme

Country Status (1)

Country Link
GB (1) GB911103A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1271660B (en) * 1964-05-26 1968-07-04 Glaxo Lab Ltd Process for the preparation of an enzyme complex

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1271660B (en) * 1964-05-26 1968-07-04 Glaxo Lab Ltd Process for the preparation of an enzyme complex

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