GB1384051A - Preparation of an enzyme product - Google Patents
Preparation of an enzyme productInfo
- Publication number
- GB1384051A GB1384051A GB323271A GB323271A GB1384051A GB 1384051 A GB1384051 A GB 1384051A GB 323271 A GB323271 A GB 323271A GB 323271 A GB323271 A GB 323271A GB 1384051 A GB1384051 A GB 1384051A
- Authority
- GB
- United Kingdom
- Prior art keywords
- enzyme
- beta
- glucanase
- endo
- freeze
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 102000004190 Enzymes Human genes 0.000 title abstract 4
- 108090000790 Enzymes Proteins 0.000 title abstract 4
- 238000002360 preparation method Methods 0.000 title abstract 2
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 abstract 4
- 241000194103 Bacillus pumilus Species 0.000 abstract 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 abstract 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 abstract 2
- 239000001166 ammonium sulphate Substances 0.000 abstract 2
- 235000011130 ammonium sulphate Nutrition 0.000 abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 2
- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 abstract 1
- 239000004382 Amylase Substances 0.000 abstract 1
- 102000013142 Amylases Human genes 0.000 abstract 1
- 108010065511 Amylases Proteins 0.000 abstract 1
- 229920002498 Beta-glucan Polymers 0.000 abstract 1
- 101710130006 Beta-glucanase Proteins 0.000 abstract 1
- 240000005979 Hordeum vulgare Species 0.000 abstract 1
- 235000007340 Hordeum vulgare Nutrition 0.000 abstract 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 abstract 1
- 235000019418 amylase Nutrition 0.000 abstract 1
- 239000006286 aqueous extract Substances 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 239000003638 chemical reducing agent Substances 0.000 abstract 1
- 239000012153 distilled water Substances 0.000 abstract 1
- 238000001035 drying Methods 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 238000004108 freeze drying Methods 0.000 abstract 1
- 235000015097 nutrients Nutrition 0.000 abstract 1
- 238000001556 precipitation Methods 0.000 abstract 1
- 238000001226 reprecipitation Methods 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
- 229910052938 sodium sulfate Inorganic materials 0.000 abstract 1
- PMZURENOXWZQFD-UHFFFAOYSA-L sodium sulphate Substances [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 abstract 1
- 235000011152 sodium sulphate Nutrition 0.000 abstract 1
- 239000002904 solvent Substances 0.000 abstract 1
- 238000001694 spray drying Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01039—Glucan endo-1,3-beta-D-glucosidase (3.2.1.39)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/244—Endo-1,3(4)-beta-glucanase (3.2.1.6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01006—Endo-1,3(4)-beta-glucanase (3.2.1.6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01071—Glucan endo-1,2-beta-glucosidase (3.2.1.71)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01073—Licheninase (3.2.1.73)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
1384051 Endo-beta-glucanase NOVO TERAPEUTISK LABORATORIUM AS 18 Jan 1972 [26 Jan 1971] 3232/71 Headings C3H and C3U [Also in Division C6] Endo-beta-glucanase is obtained by aerobic cultivation of Bacillus pumilus on a conventional nutrient medium and then isolating the enzyme for example by treating the purified and/or concentrated broth with salts for example sodium or ammonium sulphate or with a watermiscible solvent for example acetone or ethanol followed by drying the precipitated enzyme preferably by spray- or freeze-drying. The enzyme is used as a viscosity-reducing agent in brewer's wort. Strains of Bacillus pumilus exemplified:-A.T.C.C. Nos. 6632; 12,140; 98; 70; 72; 71; 6,631; 945; 4,520; 14,884; 15,477; 18; 1; 4,522 and NCIB Nos. 7,576, 8,982, 8,600, 8,738. Beta glucan is obtained from an aqueous extract of milled barley, destarched by treatment with bacterial amylase containing no betaglucanase activity, precipitation with acetone, redissolution in water and then re-precipitation with 30% ammonium sulphate. The preparation is then exhaustively dialyzed against distilled water and freeze-dried.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB323271A GB1384051A (en) | 1971-01-26 | 1971-01-26 | Preparation of an enzyme product |
| DK17972A DK131241B (en) | 1971-01-26 | 1972-01-13 | Process for the preparation of an endo-beta-glucanase product or preparation. |
| DE19722203412 DE2203412A1 (en) | 1971-01-26 | 1972-01-25 | Process for the production of an endo-beta-glucanase preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB323271A GB1384051A (en) | 1971-01-26 | 1971-01-26 | Preparation of an enzyme product |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1384051A true GB1384051A (en) | 1975-02-19 |
Family
ID=9754438
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB323271A Expired GB1384051A (en) | 1971-01-26 | 1971-01-26 | Preparation of an enzyme product |
Country Status (3)
| Country | Link |
|---|---|
| DE (1) | DE2203412A1 (en) |
| DK (1) | DK131241B (en) |
| GB (1) | GB1384051A (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NO151294C (en) * | 1982-12-22 | 1985-03-13 | Norsk Hydro As | PREPARATION OF ENZYMETE BETA-GLUCANASE FOR THE PREPARATION OF SOPHES |
-
1971
- 1971-01-26 GB GB323271A patent/GB1384051A/en not_active Expired
-
1972
- 1972-01-13 DK DK17972A patent/DK131241B/en unknown
- 1972-01-25 DE DE19722203412 patent/DE2203412A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| DK131241B (en) | 1975-06-16 |
| DK131241C (en) | 1975-11-10 |
| DE2203412A1 (en) | 1972-08-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Sawyer et al. | Biochemical characteristics of Bacteroides melaninogenicus: A study of thirty-one strains | |
| Koepsell et al. | Enzymatic synthesis of dextran | |
| Harrison | Glucose dehydrogenase: a new oxidising enzyme from animal tissues | |
| US4242326A (en) | Anti-tumor substance and process for preparing the same | |
| US4072567A (en) | Compound water-insoluble glucan and process for the production thereof | |
| GB1083662A (en) | A novel antibiotic, methods for its production and therapeutic compositions containing it | |
| GB1384179A (en) | Treatment of microorganism cells | |
| US3868464A (en) | Production of maltose with amylases produced by streptomyces | |
| Zerner et al. | Acetoacetate decarboxylase. Preparation of the enzyme | |
| IE36619L (en) | Enzymes for reducing dental plaque | |
| GB1384051A (en) | Preparation of an enzyme product | |
| GB1207781A (en) | A process for the cultivation of hemolytic streptococci | |
| GB1404516A (en) | Stabilization of microorganism cells lytic enzyme | |
| GB1221272A (en) | Process for producing cell-lytic enzymes | |
| GB1221235A (en) | A process for the production of uricase | |
| GB927321A (en) | Conversion of flavonoid substances | |
| DE2043971A1 (en) | Antitumor substance and method for obtaining it | |
| Morgan | A specific precipitating polysaccharide from B. dysenteriae (Shiga) | |
| Alfin et al. | Further studies of the action of pancreatic amylase: A differentiation of the products of the hydrolysis of potato starch and of a linear fraction from corn starch | |
| US3804717A (en) | Production of new amylases by cultivation of streptomyces and uses of these new amylases | |
| Carruthers et al. | Enzyme formation and polysaccharide synthesis by bacteria. II | |
| Stokes et al. | Stimulation of poly-β-hydroxybutyrate oxidation in Sphaerotilus discophorus by manganese and magnesium | |
| GB1333996A (en) | Production of anti-tumour substances and pharmaceutical compositions containing the same | |
| US4349541A (en) | Anti-tumor preparation against certain tumors and process for preparing the same | |
| GB1146626A (en) | Process for the manufacture of an active anticancer substance |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 416 | Proceeding under section 16 patents act 1949 | ||
| PS | Patent sealed | ||
| PCNP | Patent ceased through non-payment of renewal fee |