[go: up one dir, main page]

GB2375112A - Combinatorial protein domains - Google Patents

Combinatorial protein domains Download PDF

Info

Publication number
GB2375112A
GB2375112A GB0217404A GB0217404A GB2375112A GB 2375112 A GB2375112 A GB 2375112A GB 0217404 A GB0217404 A GB 0217404A GB 0217404 A GB0217404 A GB 0217404A GB 2375112 A GB2375112 A GB 2375112A
Authority
GB
United Kingdom
Prior art keywords
protein
chimaeric
designed
consist solely
sequence segments
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB0217404A
Other versions
GB0217404D0 (en
Inventor
Lutz Riechmann
Greg Winter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Domantis Ltd
Original Assignee
Domantis Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0002492A external-priority patent/GB0002492D0/en
Priority claimed from GB0016346A external-priority patent/GB0016346D0/en
Priority claimed from GB0019362A external-priority patent/GB0019362D0/en
Application filed by Domantis Ltd filed Critical Domantis Ltd
Publication of GB0217404D0 publication Critical patent/GB0217404D0/en
Publication of GB2375112A publication Critical patent/GB2375112A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

The present invention relates to a chimaeric folded protein domain comprising two or more sequence segments from parent amino acid sequences that are not homologous, wherein each of said sequence segments: is not designed or selected to consist solely of a single complete protein structural element and is not designed or selected to consist solely of an entire protein domain; and, in isolation, shows no significant folding at the melting temperature of the chimaeric protein; as well as to methods for the selection of such domains; and to a chimaeric folded protein domain comprising two or more sequence segments which share common sequences or sequences from common regions in the protein fold of their parent amino acid sequences, wherein the said common region of the fold is not designed or selected to consist solely of one or more complete protein structural elements, and wherein each of said sequence segments: is not designed or selected to consist solely of an entire protein domain; and wherein, in isolation, each said sequence segment shows no significant folding at the melting temperature of the chimaeric protein; as well as to methods for the selection of such domains.

Description

(57) The present invention relates to a chimaeric folded protein domain
comprising two or more sequence segments from parent amino acid sequences that are not homologous, wherein each of said sequence segments: is not designed or selected to consist solely of a single complete protein structural element and is not designed or selected to consist solely of an entire protein domain; and, in isolation, shows no significant folding at the melting temperature of the chimaeric protein; as well as to methods for the selection of such domains; and to a chimaeric folded protein domain comprising two or more sequence segments which share common sequences or sequences from common regions in the protein fold of their parent amino acid sequences, wherein the said common region of the fold is not designed or selected to consist solely of one or more complete protein structural elements, and wherein each of said sequence segments: is not designed or selected to consist solely of an entire protein domain; and wherein, in isolation, each said sequence segment shows no significant folding at the melting temperature of the chimaeric protein; as well as to methods for the selection of such domains.
GB0217404A 2000-02-03 2001-02-02 Combinatorial protein domains Withdrawn GB2375112A (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GB0002492A GB0002492D0 (en) 2000-02-03 2000-02-03 Combinatorial protein domains
US18032600P 2000-02-04 2000-02-04
GB0016346A GB0016346D0 (en) 2000-07-03 2000-07-03 Combinatorial protein domains
GB0019362A GB0019362D0 (en) 2000-08-07 2000-08-07 Combinational protein Domains
PCT/GB2001/000445 WO2001057065A2 (en) 2000-02-03 2001-02-02 Combinatorial protein domains

Publications (2)

Publication Number Publication Date
GB0217404D0 GB0217404D0 (en) 2002-09-04
GB2375112A true GB2375112A (en) 2002-11-06

Family

ID=27447768

Family Applications (1)

Application Number Title Priority Date Filing Date
GB0217404A Withdrawn GB2375112A (en) 2000-02-03 2001-02-02 Combinatorial protein domains

Country Status (7)

Country Link
EP (1) EP1252319A2 (en)
JP (1) JP2003523742A (en)
AU (1) AU3040101A (en)
CA (1) CA2399809A1 (en)
GB (1) GB2375112A (en)
NO (1) NO20023606L (en)
WO (1) WO2001057065A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2525120C (en) 2003-05-14 2013-04-30 Domantis Limited A process for recovering polypeptides that unfold reversibly from a polypeptide repertoire
MX2009013137A (en) 2007-06-06 2010-04-30 Domantis Ltd Methods for selecting protease resistant polypeptides.
WO2009068649A2 (en) 2007-11-30 2009-06-04 Glaxo Group Limited Antigen-binding constructs
MX2011005874A (en) 2008-12-05 2011-06-27 Glaxo Group Ltd Methods for selecting protease resistant polypeptides.
WO2010097394A1 (en) 2009-02-24 2010-09-02 Glaxo Group Limited Multivalent and/or multispecific rankl-binding constructs
CA2753287A1 (en) 2009-02-24 2010-09-02 Glaxo Group Limited Antigen-binding constructs
US20110305693A1 (en) 2009-02-24 2011-12-15 Glaxo Group Limited Anitigen-binding constructs
AU2011333738A1 (en) 2010-11-24 2013-07-11 Glaxo Group Limited Multispecific antigen binding proteins targeting HGF
EP2736925A2 (en) 2011-07-27 2014-06-04 Glaxo Group Limited Anti-vegf single variable domains fused to fc domains
EP3561703B1 (en) * 2018-04-25 2021-01-20 Bayer AG Identification of the mating of variable domains from light and heavy chains of antibodies
JPWO2023140360A1 (en) * 2022-01-20 2023-07-27

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995021914A1 (en) * 1994-02-10 1995-08-17 Pasteur Merieux Serums Et Vaccins Method for preparing a multicombinatorial library of antibody gene expression vectors
WO1997032017A1 (en) * 1996-02-26 1997-09-04 Morphosys Gesellschaft Für Proteinoptimierung Mbh Novel method for the identification of nucleic acid sequences encoding two or more interacting (poly)peptides
WO1999058655A2 (en) * 1998-05-13 1999-11-18 Diversys Limited Phage display selection system for folded proteins
WO2001042432A2 (en) * 1999-12-08 2001-06-14 Medical Research Council Modified enzymatic activity through subdomain swaps between related alpha/beta-barrel enzymes

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995021914A1 (en) * 1994-02-10 1995-08-17 Pasteur Merieux Serums Et Vaccins Method for preparing a multicombinatorial library of antibody gene expression vectors
WO1997032017A1 (en) * 1996-02-26 1997-09-04 Morphosys Gesellschaft Für Proteinoptimierung Mbh Novel method for the identification of nucleic acid sequences encoding two or more interacting (poly)peptides
WO1999058655A2 (en) * 1998-05-13 1999-11-18 Diversys Limited Phage display selection system for folded proteins
WO2001042432A2 (en) * 1999-12-08 2001-06-14 Medical Research Council Modified enzymatic activity through subdomain swaps between related alpha/beta-barrel enzymes

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
Chem. & Biology 1999, 8, pp.R91-R105 - O'Brien et al. *
Gene 2000, 242(2), pp.369-379 - Flajolet et al. *
Nature 1998, 391, pp.288-291 - Crameri et al. *
Nature 2000, 403, pp.617-622 - Altamirano et al. *
Proc. Natl. Acad. Sci. USA 1998, 95, pp.9813-9818 - Hopfner et al. *
Proc. Natl. Acad. Sci. USA 1999, 96, pp.3562-3567 - Ostermeier et al. *
Proc. Natl. Acad. Sci. USA 2000, 97(18), pp.10068-73 - Riechmann et al. *

Also Published As

Publication number Publication date
GB0217404D0 (en) 2002-09-04
AU3040101A (en) 2001-08-14
WO2001057065A3 (en) 2002-01-31
WO2001057065A2 (en) 2001-08-09
EP1252319A2 (en) 2002-10-30
NO20023606L (en) 2002-10-02
NO20023606D0 (en) 2002-07-30
CA2399809A1 (en) 2001-08-09
JP2003523742A (en) 2003-08-12

Similar Documents

Publication Publication Date Title
GB2375112A (en) Combinatorial protein domains
YU8997A (en) Purified protein having serinic proteaze inhibiting activity and pharmaceutical preparation containing them
TR199900267T2 (en) GnRH-L�kotoksin kimeralar�.
NO20003958L (en) Tumor associated antigen derivatives from the MAGE family, and nucleic acid sequences encoding them, used for the production of fusion proteins and for vaccine compositions
NO20030443D0 (en) Process for the preparation of functional protein domains
WO2003031464A3 (en) Remodeling and glycoconjugation of peptides
WO2001038547A3 (en) Polypeptides comprising multimers of nuclear localization signals or of protein transduction domains and their use for transferring molecules into cells
WO1999015549A3 (en) Peptide sequences as hinge regions in proteins like immunoglobulin fragments and their use in medicine
TR200102277T2 (en) Three-ring (Tricyclic) pyrazole derivatives
AU2002217010A1 (en) Peptides, the production and use thereof for binding immunoglobulins
WO1995006735A3 (en) Nucleotide sequences for novel protein tyrosine phosphatases
DK1025237T3 (en) Human checkpoint kinase, hCDS1, preparations and methods
AU4367196A (en) Novel protein domain which binds tyrosine phosphorylated proteins
WO1999024452A3 (en) Pyrimidine derivatives for labeled binding partners
WO1997011968A3 (en) A gene associated with liver neoplastic disease
ATE305037T1 (en) PEPTIDES CAPABLE OF BINDING THE SH3 DOMAIN OF THE GAP PROTEIN, ITS CODING DNA, THEIR PREPARATION AND USE
MX9806041A (en) PURIFIED SR-p70 PROTEIN.
DE69333952D1 (en) CLONING OF THE GENE FOR T GONDII PROTEIN GP 28.5 PEPTIDE FRAGMENTS FROM THIS PROTEIN AND THEIR USES.
WO2002055547A3 (en) Sfrp and peptide motifs that interact with sfrp and methods of their use
AU2001238064A1 (en) 18477, a human protein kinase and uses therefor
DE69938130D1 (en) NEW PEPTIDE
WO1999022005A3 (en) Heparan sulfate d-glucosaminyl 3-o-sulfotransferases, an uses therefor
AR022150A1 (en) NUCLEIC ACIDS DERIVED FROM MONOCITS AND RELATED COMPOSITIONS AND METHODS
WO2002012277A3 (en) Hybrid combinatorial proteins made from reshuffling of differently folded domains
WO1998017827A3 (en) Compositions and methods using eukaryotic rad52

Legal Events

Date Code Title Description
WAP Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1)