[go: up one dir, main page]

GB2262038A - Ovicidal compositions - Google Patents

Ovicidal compositions Download PDF

Info

Publication number
GB2262038A
GB2262038A GB9225531A GB9225531A GB2262038A GB 2262038 A GB2262038 A GB 2262038A GB 9225531 A GB9225531 A GB 9225531A GB 9225531 A GB9225531 A GB 9225531A GB 2262038 A GB2262038 A GB 2262038A
Authority
GB
United Kingdom
Prior art keywords
eggs
composition according
phenols
oocysts
composition
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB9225531A
Other versions
GB2262038B (en
GB9225531D0 (en
Inventor
Humphrey Richard Ian Cannon
Patricia Joan Godfrey
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Armillatox Ltd
Original Assignee
Armillatox Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB919126007A external-priority patent/GB9126007D0/en
Application filed by Armillatox Ltd filed Critical Armillatox Ltd
Priority to GB9225531A priority Critical patent/GB2262038B/en
Publication of GB9225531D0 publication Critical patent/GB9225531D0/en
Publication of GB2262038A publication Critical patent/GB2262038A/en
Application granted granted Critical
Publication of GB2262038B publication Critical patent/GB2262038B/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/08Oxygen or sulfur directly attached to an aromatic ring system

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

An ovicidal composition comprises an aqueous emulsion of phenols, preferably alkyl phenols, with a soap such as saponified caster oil. The composition may be used in the treatment of toxocara canis eggs and other infectious forms of other dog and cat worm eggs, thus assisting in the prevention of toxocariasis and the like. The diluted composition may be applied directly to grass and soil on which the eggs are commonly found.

Description

Ovicidal Compositions This invention concerns ovicidal compositions.
Considerable publicity has been given recently to the incidence of human toxocariasis, a disease which can cause a number of clinical symptons, including blindness.
The disease is caused by a parasite known as toxocara, and may be spread in dog excrement. Toxocara eggs may survive in soil for prolonged periods, and may be accidentally ingested by children when playing in parks, for example.
A further disease known as toxoplasmosis may be transmitted by domestic pets, usually cats. The disease is caused by a parasite known as toxoplasma gondii, and although in the majority of cases it is not serious, it may be dangerous particularly in pregnant women (where the unborn baby may be affected) and in patients whose defences against infection are impaired. Toxoplasmosis is carried by an infectious cyst form of toxoplasma known as an oocyst which may be accidentally ingested, again primarily by soil contamination.
Although the observance of certain hygiene precautions can reduce the risk of infection, it would naturally be preferable to eradicate the source of infection, namely the toxocara eggs or the toxoplasma oocysts. The toxocara egg is particularly resistant, and various known disinfectants have been found ineffective in killing the eggs, particularly when applied at concentrations which are not phytotoxic - that is, which will not damage grass and park land on which the eggs are found.
The present invention seeks to provide ovicidal compositions which are effective in destroying toxocara eggs and toxoplasma oocysts, but which are not phytotoxic.
The terms "egg" and Itovicidell when used in this specification are to be understood to include oocysts and substances which are capable of destroying oocysts.
According to the invention there is provided a composition for use as an ovicide comprising an aqueous emulsion of phenols as defined hereinafter with a soap.
The term "phenols'1 when used in this specification includes all aryl hydroxides, and is not limited to hydroxy benzenes.
Preferably there is used a mixture predominantly of high boiling alkyl phenols. In particular there may be employed a mixture of phenols derived from coal carbonisation. Preferably the phenol mixture has a boiling range of 220 - 2900C, and more preferably of 230 - 2800C, and a specific gravity of approximately 1.023 at 15 C.
The soap is preferably saponified castor oil.
The ovicidal composition preferably comprises at least 20E ó by volume of phenols and up to 80, ó by volume of soap.
The ovicidal composition more preferably comprises 30 - 35 by volume of phenols and 70 - 65 by volume of soap.
In a further aspect, the invention is the use of an aqueous emulsion of phenols as hereinbefore defined as an ovicide. In particular, the invention provides for such use in the ovicidal treatment of helminth ova, protozoal oocysts or platyhelminth eggs, such as toxocara canis eggs, toxoplasma gondii oocysts or Bilharzia eggs.
In a still further aspect, the invention relates to the use of a composition as hereinbefore defined in the manufacture of ovicidal preparations.
In a yet further aspect, the invention provides an ovicidal method comprising the steps of providing an aqueous solution of a composition as hereinbefore defined and applying the solution to an area contaminated with helminth ova, protozoal oocysts or platyhelminth eggs.
The invention also provides a method of preventive control of helminth ova, protozoal oocysts and platyhelminth eggs comprising the steps of providing an aqueous solution of a composition as hereinbefore defined and applying the solution to an area liable to contamination with the ova, oocysts or eggs.
The invention will be further described for the purposes of illustration only with reference to the following example.
Example An ovicidal composition according to the invention is prepared according to the following formulation.
a) Castor oil soap (70 a) The soap is saponified castor oil. Physical properties of the castor oil are as follows: specific gravity 0.969 at 15.50C; saponification value 183 - 186; refractive index 1.480 at 150C; iodine value 82 - 86.
b) Phenol mixture (30C a) as follows: 7 3-methyl 5-ethyl phenol 2 ó 2,3,5-trimethyl phenol 5 propyl phenols 5% indanols methyl indanols 2 ó 3,4-xylenol 73 Ó other alkyl phenols.
The product is a dark brown to black clear viscous liquid having a specific gravity at 600F (15.560C) of approximately 1.027 and pH 7.5.
Initial studies using aqueous solutions of the product at varying dilutions between 1:50 and 1:300 applied to solutions of toxocara canis in dilute formalin solution have indicated that the product is effective in destroying the eggs and/or preventing embryonation as compared to control samples in water incubated under the same conditions.
The composition may also be used in the treatment of toxoplasma gondii oocysts, and the eggs of Bilharzia, which cause bilharziasis (schistosomiasis).
In use the concentrated product is diluted 1:200 with water. The diluted solution is applied to lawns, soil and the like which are suspected of contamination.
Compositions according to the invention may also be used directly to sterilize deposits of excrement.
The preferred degree of dilution of the product may vary depending upon the circumstances of use.
The product is biodegradeable and is not phytotoxic.
The following trial further illustrates the invention.
An exercise paddock, of approximately 150 sq.m, at a commercial greyhound breeding kennels was used for the trial.
Two weeks prior to the start of the trial soil samples were collected randomly from the paddock and examined for toxocara canis and other dog worm eggs, to confirm the degree of contamination.
rive separate test areas were then designated within the paddock and the composition of the invention was applied using a watering can according to the Schedule outlined in Table 1.
TABLE 1 Reference Rate of Dose Number Letter Dilution ~ Application (weekly interval) C 1:50 5 litre/sq.m 1 1:50 5 litre/sq.m 2 E 1:100 5 litre/4 sq.m 1 1:100 5 litre/4 sq.m 2 A 1:100 5 litre/4 sq.m 1 1:100 10 litre/4 sq.m 2 D 1:200 5 litre/4 sq.m 1 1:200 5 litre/4 sq.m 2 B 1:200 5 litre/4 sq.m 1 1:200 10 litre/4 sq.m 2 The ovicidal composition was diluted as indicated using tap water.
Soil samples were collected from each test area and examined for toxocara canis and other worm eggs, according to the following timetable.
Trial Day Action Taken Control samples 1-8 collected.
1 First dose applied.
2 First soil samples collected: Al, B1, C1, D1, El.
3 Samples A1-E1 processed.
7 Soil samples collected: A2, 82, C2, D2, E2, the second dose applied.
8 Collect soil samples: A3, B3, C3, D3, E3.
Process samples: A2-E2 9 Process samples: A3-E3 Method Soil samples were taken using a gardening trowel, scraping approximately 100 g into labelled polythene bags. A salt flotation method was used to extract the eggs from the soil (modified from Quinn et al). The soil sample was sieved through a 4 mm sieve to remove stones, grass and other large matter. In a small beaker to which 100 ml of a detergent and wetting agent was added, to separate the worm eggs from the soil particles. The soil suspension was then homogenised in a heavy-duty laboratory homogeniser for 5 minutes. The suspension was then poured into four centrifuge tubes and spun at 2000 rpm for 10 minutes. The supernatant was discarded and the sediment was resuspended in saturated magnesium sulphate solution, to float the eggs. The tubes were again centrifuged at 2000 rpm for a further 10 minutes.Finally the tubes were topped up with magnesium sulphate solution to make a positive meniscus. A cover slip was placed on each of the four tubes and left for 5 minutes while the eggs accumulated under it. Each cover slip was then transferred to a microscope slide and examined at x100 magnification. The number of eggs per sample was the total number found on all four slides.
Results The results are set out in Table 2.
TABLE 2 Numbers of T. canis (and T. vulpis) eggs found in 25 g soil samples.
SAMPLE NUMBER NUMBER OF EGGS T. canis T. Volpis CONTROLS 1 1 1 2 8 2 3 4 6 4 5 6 5 9 4 6 0 0 7 8 2 8 5 0 (Toxocariasis leonina eggs were also found in samples 1 and 6).
SAMPLES TAKEN AFTER FIRST ARMILLATOX DOSE: 1:100 Al 17 5 1:200 B1 No sample 1:50 C1 1 8 1:200 Dl No sample 1:100 El 3 6 SAMPLES TAKEN ONE WEEK AFTER FIRST ARMILLATOX DOSE: 1:100 A2 14 6 1:200 B2 5 3 1:50 C2 1 3 1:200 D2 0 3 1:100 E2 2 1 SAMPLES TAKEN AFTER SECOND ARMILLATOX DOSE: 1:100 A3 5 0 1:200 B3 4 2 1:50 C3 1 4 1:200 D3 0 6 1:100 E3 0 2 Observations Although the scope of the initial trial is limited, there appears to be a decrease in the number of toxocara canis eggs found in the final set of samples, after two doses of the treatment.
In the test samples A and B the dosage was doubled for the second dose as the degree of soil wetting and penetration was not great before the initial application rate of 5 litre/4 sq.m.
The number of eggs found in area A, in particular, was greatly reduced and no eggs were found in areas D and E.
Other Phenols A suitable phenol mixture for use in the invention may be derived from natural sources, and accordingly the composition of the phenol mixture may be varied within the limits set out hereinbefore. The mixture may for example include indanols and alkyl indanols, catechol and alkyl substituted catechols, hydroxy hydrindenes and beta napthol.
Analysis of a typical phenol mixture may isolate any or all of the following: M.N. propyl phenol 2,3,5-trimethyl phenol 2,4,5-trimethyl phenol 3-methyl 5-ethyl phenol catechol 4-hydroxy hydrindene 3-methyl catechol 3,6-dimethyl catechol 5-hydroxy hydrindene 4-methyl catechol 3,5-di methyl catechol beta napthol.
The invention accordingly provides a composition which may be diluted with water and can then be applied directly to areas of soil, grassland and the like from which toxocariasis and/or toxoplasmosis might be contracted, without risk of damage to grass or plants.
Whilst endeavouring in the foregoing specification to draw attention to those features of the invention believed to be of particular importance it should be understood that the Applicant claims protection in respect of any patentable feature or combination of features hereinbefore referred to whether or not particular emphasis has been placed thereon.

Claims (19)

Claims:
1. A composition for use as an ovicide comprising an aqueous emulsion of phenols with a soap.
2. A composition according to Claim 1, wherein the phenols are alkyl phenols.
3. A composition according to Claim 1, wherein the phenols comprise a phenol mixture derived from coal carbonisation.
4. A composition according to any of the preceding Claims, wherein there is used a phenol mixture having a boiling range of 220-2800C.
5. A composition according to any of the preceding Claims, wherein there is used a phenol mixture having a boiling range of 230-2800C.
6. A composition according to any of the preceding Claims, wherein the soap is Saponified castor oil.
7. A composition according to any of the preceding Claims, comprising at least 20 ó by volume of phenols and up to 80 ó by volume of soap.
8. A composition according to Claim 7, comprising 30-35 by volume of phenols and 70-65 ó by volume of soap.
9. The use of a composition according to any of Claims 1 to 8 as an ovicide.
10. The use according to Claim 9, in the ovicidal treatment of helminth ova, protozoal oocysts or platyhelminth eggs.
11. The use according to Claim 10 in the treatment of toxocara canis eggs.
12. The use according to Claim 10 in the treatment of toxoplasma gondii oocysts.
13. The use according to Claim 10 in the treatment of Bilharzia eggs.
14. An ovicidal method comprising the steps of providing an aqueous solution of a composition according to any of Claims 1 to 8 and applying the solution to an area contaminated with helminth ova, protzoal oocysts or platyhelminth eggs.
15. A method of preventive control of helminth ova, protozoal oocysts and platyhelminth eggs comprising the steps of providing an aqueous solution of a composition according to any of Claims 1 to 8 and applying the solution to an area liable to contamination with the ova oocysts or eggs.
16. The use of a composition according to any of Claims 1 to 8 in the manufacture of ovicidal preparations.
17. A composition substantially as hereinbefore described.
18. A method substantially as hereinbefore described.
19. Any novel subject matter or combination including novel subject matter disclosed, whether or not within the scope of or relating to the same invention as any of the preceding Claims.
GB9225531A 1991-12-06 1992-12-07 Ovicidal methods Expired - Fee Related GB2262038B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB9225531A GB2262038B (en) 1991-12-06 1992-12-07 Ovicidal methods

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB919126007A GB9126007D0 (en) 1991-12-06 1991-12-06 Ovicidal compositions
GB9225531A GB2262038B (en) 1991-12-06 1992-12-07 Ovicidal methods

Publications (3)

Publication Number Publication Date
GB9225531D0 GB9225531D0 (en) 1993-01-27
GB2262038A true GB2262038A (en) 1993-06-09
GB2262038B GB2262038B (en) 1996-01-31

Family

ID=26299970

Family Applications (1)

Application Number Title Priority Date Filing Date
GB9225531A Expired - Fee Related GB2262038B (en) 1991-12-06 1992-12-07 Ovicidal methods

Country Status (1)

Country Link
GB (1) GB2262038B (en)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1060134A (en) * 1964-12-29 1967-03-01 Coalite Chem Prod Ltd Bactericidal compositions
GB1067539A (en) * 1965-03-31 1967-05-03 Unilever Ltd Antimicrobial composition
GB1090020A (en) * 1965-10-22 1967-11-08 Colgate Palmolive Co Antibacterial compositions
GB1171943A (en) * 1965-11-18 1969-11-26 Arrowhead Ind Inc Deodorant.
GB1420946A (en) * 1972-10-13 1976-01-14 Beecham Group Ltd Anti-bacterial compositions
SU682202A1 (en) * 1977-12-05 1979-08-30 Ленинградский Научно-Исследовательский Институт Лесного Хозяйства Soil reclamation method

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1060134A (en) * 1964-12-29 1967-03-01 Coalite Chem Prod Ltd Bactericidal compositions
GB1067539A (en) * 1965-03-31 1967-05-03 Unilever Ltd Antimicrobial composition
GB1090020A (en) * 1965-10-22 1967-11-08 Colgate Palmolive Co Antibacterial compositions
GB1171943A (en) * 1965-11-18 1969-11-26 Arrowhead Ind Inc Deodorant.
GB1420946A (en) * 1972-10-13 1976-01-14 Beecham Group Ltd Anti-bacterial compositions
SU682202A1 (en) * 1977-12-05 1979-08-30 Ленинградский Научно-Исследовательский Институт Лесного Хозяйства Soil reclamation method

Also Published As

Publication number Publication date
GB2262038B (en) 1996-01-31
GB9225531D0 (en) 1993-01-27

Similar Documents

Publication Publication Date Title
Bornstein et al. Sarcoptes scabiei and sarcoptic mange
Szwabe et al. Stray dogs and cats as potential sources of soil contamination with zoonotic parasites
Munene et al. Helminth and protozoan gastrointestinal tract parasites in captive and wild-trapped African non-human primates
Moyo et al. Tick control methods used by resource-limited farmers and the effect of ticks on cattle in rural areas of the Eastern Cape Province, South Africa
WO2010061354A2 (en) Insect-repellent formulations
US20100196520A1 (en) Insect repellent formulations
Zubairi et al. Environmental friendly bio-pesticide Rotenone extracted from Derris sp.: A review on the extraction method, toxicity and field effectiveness
Satriawan et al. Toxicity of the organophosphorus pesticide temephos
GB2262038A (en) Ovicidal compositions
Pellegrino et al. Oogram Studies with Hycanthone®, a New Antischistosomal Agent
Madhina Prevention of snail miracidia interactions using Phytolacca dodecandra (L'Herit)(endod) as a miracidiacide: an alternative approach to the focal control of schistosomiasis
CN112237187B (en) Application of phenolic compound as nematicide
CN106635415A (en) Fly-repelling vegetable essential oil and preparation method and application thereof
Khan Impact of endosulfan on living beings
Arruda et al. Correspondence between urban areas and the concentrations of chlordane in fish from the Kansas River
Shekhawat et al. Molluscicidal activity of some Indian medicinal plants against the snail Lymnaea acuminata and in the control of fascioliasis
McNeil Jr The use of benzene hexachloride as a copepodicide and some observations on lernaean parasites in trout rearing units
Abou-Eisha et al. Prevalence of some zoonotic parasites in dog faecal deposits in Ismailia City
Uikey Effect of malathion toxicity on fresh water fish Labeo rohita
ABOU THE EFFECT OF AMBROSIA MARITIMA (DAMSISSA) ON THE VIABILITY OF LYMNAEA CAILLIAUDI; AN EXP-ERIMENTAL STUDY.
Solyman et al. Assessment of Lethal Dose and Lethal Time of Diazinon in Swiss Albino Mice
TOFEL et al. Bioactivity of Areca catechu seed extracts and Azadirachta indica seed oil against Bulinus sp., an intermediate host of Schistosoma haematobium
Daben et al. Effects of n-hexane, Ethyl Acetate and Methanol bark extracts of Entada africana Guill. & Perott.(Fabaceae) on the Snail Bulinus globosus.
Intisar et al. Anthelmintic activity of Zizyphus spina-christi leaves
Kanwal et al. Toxicological Studies of Cyhalothrin (Pyrethroid) and Monochrotophos (Organophosphate) on Total Protein Contents in Liver, Kidney, and Muscles of Coturnix coturnix.

Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee

Effective date: 20111207