GB2102291A - Method for purifying polyosides of streptococcus pneumoniae and vaccine based on polyosides thus purified - Google Patents
Method for purifying polyosides of streptococcus pneumoniae and vaccine based on polyosides thus purifiedInfo
- Publication number
- GB2102291A GB2102291A GB08223061A GB8223061A GB2102291A GB 2102291 A GB2102291 A GB 2102291A GB 08223061 A GB08223061 A GB 08223061A GB 8223061 A GB8223061 A GB 8223061A GB 2102291 A GB2102291 A GB 2102291A
- Authority
- GB
- United Kingdom
- Prior art keywords
- polyosides
- streptococcus pneumoniae
- purifying
- purified
- vaccine based
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000193998 Streptococcus pneumoniae Species 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 229940031000 streptococcus pneumoniae Drugs 0.000 title abstract 2
- 229960005486 vaccine Drugs 0.000 title 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 abstract 2
- 239000008346 aqueous phase Substances 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 238000005352 clarification Methods 0.000 abstract 1
- 230000009089 cytolysis Effects 0.000 abstract 1
- 239000012535 impurity Substances 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/02—Bacterial antigens
- A61K39/09—Lactobacillales, e.g. aerococcus, enterococcus, lactobacillus, lactococcus, streptococcus
- A61K39/092—Streptococcus
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Peptides Or Proteins (AREA)
Abstract
The method is characterized by the fact that the starting protein is an aqueous solution of said polyosides, containing protein impurities, obtained by lysis of a culture of Streptococcus pneumoniae, clarification and concentration of the polyoside, that, with the aim to reduce the protein content, there are added 5 to 50% of phenol by volume, at a temperature comprised between 5 and 30`C approximately, that the aqueous phase is first stirred, and then separated and collected, and that the phenol treatment, if necessary, is repeated until the protein content of the aqueous phase is lower than a predetermined threshold.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR8026320A FR2495939B1 (en) | 1980-12-11 | 1980-12-11 | PROCESS FOR THE PURIFICATION OF STREPTOCOCCUS PNEUMONIAE POLYOSIDES AND VACCINE BASED ON THE PURIFIED POLYOSIDES |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| GB2102291A true GB2102291A (en) | 1983-02-02 |
| GB2102291B GB2102291B (en) | 1985-07-24 |
Family
ID=9248957
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB08223061A Expired GB2102291B (en) | 1980-12-11 | 1981-12-11 | Method for purifying polyosides of streptococcus pneumoniae and vaccine based on polyosides thus purified |
Country Status (7)
| Country | Link |
|---|---|
| BE (1) | BE891444A (en) |
| CH (1) | CH662056A5 (en) |
| DE (1) | DE3152621T1 (en) |
| FR (1) | FR2495939B1 (en) |
| GB (1) | GB2102291B (en) |
| NL (1) | NL8120466A (en) |
| WO (1) | WO1982001995A1 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5623057A (en) * | 1991-01-28 | 1997-04-22 | Merck & Co., Inc. | Pneumococcal polysaccharide conjugate vaccine |
| RU2121481C1 (en) * | 1988-12-16 | 1998-11-10 | Де Стат Дер Недерланден Вертегенвордигд Дор де Министр Ван Велзейн, Волксгезондхейд эн Кюлтюр | Modified pheumolysine recombinant plasmid, method of producing modified pheumolysine and vaccine |
| US5847112A (en) * | 1991-01-28 | 1998-12-08 | Merck & Co., Inc. | Process for making capsular polysaccharides from Streptococcus pneumoniae |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| HUE050049T2 (en) * | 2005-04-08 | 2020-11-30 | Wyeth Llc | Separation of contaminants from streptococcus pneumoniae polysaccharide by ph manipulation |
| UA99278C2 (en) * | 2007-03-23 | 2012-08-10 | УАЙЕТ ЭлЭлСи | Shortened purification process for the production of capsular streptococcus pneumoniae polysaccharides |
| US8795689B2 (en) | 2008-12-18 | 2014-08-05 | Wyeth Llc | Method for controlling Streptococcus pneumoniae serotype 19A polysaccharide molecular weight |
| JP5789514B2 (en) | 2008-12-18 | 2015-10-07 | ワイス・エルエルシー | Method for controlling the molecular weight of Streptococcus pneumoniae polysaccharide using carbon dioxide |
| WO2011151841A1 (en) * | 2010-05-31 | 2011-12-08 | Panacea Biotec Limited | Fermentation process for streptococcus pneumoniae |
| CN106460062A (en) | 2014-05-05 | 2017-02-22 | 美敦力公司 | Methods and compositions for SCD, CRT, CRT-D or SCA treatment identification and/or selection |
| GB202016165D0 (en) | 2020-10-12 | 2020-11-25 | Optivalent Ltd | Vaccine |
| GB2614916A (en) | 2022-01-25 | 2023-07-26 | Optivalent Ltd | Intradermal vaccine complement |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2388563A1 (en) * | 1977-04-29 | 1978-11-24 | Fabre Sa Pierre | ADVANCED ACELLULAR VACCINES CONTAINING CAPSULAR POLYSACCHARIDES |
-
1980
- 1980-12-11 FR FR8026320A patent/FR2495939B1/en not_active Expired
-
1981
- 1981-12-11 NL NL8120466A patent/NL8120466A/en unknown
- 1981-12-11 DE DE813152621T patent/DE3152621T1/en active Granted
- 1981-12-11 WO PCT/FR1981/000161 patent/WO1982001995A1/en not_active Ceased
- 1981-12-11 CH CH4795/82A patent/CH662056A5/en not_active IP Right Cessation
- 1981-12-11 GB GB08223061A patent/GB2102291B/en not_active Expired
- 1981-12-11 BE BE0/206808A patent/BE891444A/en not_active IP Right Cessation
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2121481C1 (en) * | 1988-12-16 | 1998-11-10 | Де Стат Дер Недерланден Вертегенвордигд Дор де Министр Ван Велзейн, Волксгезондхейд эн Кюлтюр | Modified pheumolysine recombinant plasmid, method of producing modified pheumolysine and vaccine |
| US5623057A (en) * | 1991-01-28 | 1997-04-22 | Merck & Co., Inc. | Pneumococcal polysaccharide conjugate vaccine |
| US5847112A (en) * | 1991-01-28 | 1998-12-08 | Merck & Co., Inc. | Process for making capsular polysaccharides from Streptococcus pneumoniae |
Also Published As
| Publication number | Publication date |
|---|---|
| CH662056A5 (en) | 1987-09-15 |
| BE891444A (en) | 1982-06-11 |
| FR2495939B1 (en) | 1985-10-11 |
| FR2495939A1 (en) | 1982-06-18 |
| DE3152621T1 (en) | 1983-11-17 |
| WO1982001995A1 (en) | 1982-06-24 |
| NL8120466A (en) | 1982-11-01 |
| DE3152621C2 (en) | 1988-05-11 |
| GB2102291B (en) | 1985-07-24 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PE20 | Patent expired after termination of 20 years |
Effective date: 20011210 |