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GB2199325A - 16-dehydro-20-oxopregnane derivatives - Google Patents

16-dehydro-20-oxopregnane derivatives Download PDF

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GB2199325A
GB2199325A GB08730269A GB8730269A GB2199325A GB 2199325 A GB2199325 A GB 2199325A GB 08730269 A GB08730269 A GB 08730269A GB 8730269 A GB8730269 A GB 8730269A GB 2199325 A GB2199325 A GB 2199325A
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dinor
oxo
oic acid
dione
dehydro
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GB8730269D0 (en
GB2199325B (en
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Andras Toro
Gabor Ambrus
Nandor Makk
Tibor Lang
Eva Ilkoy
Lajos Toldy
Antonia Jekkel
Sarudy Eva Toth
Gyula Horvath
Karoly Albrecht
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Richter Gedeon Vegyeszeti Gyar Nyrt
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Richter Gedeon Vegyeszeti Gyar RT
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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07JSTEROIDS
    • C07J21/00Normal steroids containing carbon, hydrogen, halogen or oxygen having an oxygen-containing hetero ring spiro-condensed with the cyclopenta(a)hydrophenanthrene skeleton
    • C07J21/005Ketals
    • C07J21/006Ketals at position 3
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07JSTEROIDS
    • C07J13/00Normal steroids containing carbon, hydrogen, halogen or oxygen having a carbon-to-carbon double bond from or to position 17
    • C07J13/005Normal steroids containing carbon, hydrogen, halogen or oxygen having a carbon-to-carbon double bond from or to position 17 with double bond in position 16 (17)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07JSTEROIDS
    • C07J13/00Normal steroids containing carbon, hydrogen, halogen or oxygen having a carbon-to-carbon double bond from or to position 17
    • C07J13/007Normal steroids containing carbon, hydrogen, halogen or oxygen having a carbon-to-carbon double bond from or to position 17 with double bond in position 17 (20)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07JSTEROIDS
    • C07J41/00Normal steroids containing one or more nitrogen atoms not belonging to a hetero ring
    • C07J41/0005Normal steroids containing one or more nitrogen atoms not belonging to a hetero ring the nitrogen atom being directly linked to the cyclopenta(a)hydro phenanthrene skeleton
    • C07J41/0016Oximes

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Steroid Compounds (AREA)
  • Catalysts (AREA)
  • Low-Molecular Organic Synthesis Reactions Using Catalysts (AREA)

Abstract

A new process for preparing 16-dehydro-20-oxopregnane derivatives characterized by reacting a steroid having 17(20)-dehydro-23,24-dinorcholan-22-oic acid structure or a salt thereof with hydrogen peroxide in neutral or alkaline medium in the presence of salts of oxo-acids of transition metals as catalysts. Compounds prepared include the novel compounds 9 alpha -hydroxy-4, 16-pregnadiene-3,20-dione; 1,4,9(11), 16-pregnatetraene-3,20-dione; 3-methoxyimino-4, 9(11), 16-pregnatrien-20-one and 5,9(11), 16-pregratriene-3, 20-dione 3-ethylene ketal.

Description

PROCESS FOR PREPARING 16-DEHYDRO-20-OXIPREGNANE DERIVATIVES The invention relates to a new process for preparing 16-dehydro-20-oxopregnane derivatives.
Since 1950, 16-dehydro-20-oxopregnane derivatives have been used as intermediates in the synthesis of corticosteroids / P. L. Julian et awl. : J. Am. Chem. Soc.
72, 5145 (1950)7. They may be advantageously prepared by the oxidative chemical degradation of various sapcgenines, e.g. diosgenine, tigogenine or hecogenine / R. E. Marker et al.--: 3. Am. Chem. Soc. 61, 3592 (1939); ibid. 62, 51E (1940); ibid. 69, 2167 (1947)7 as well as from the aglycones of some steroidal alkaloids, e.g. solasodine or tomatidine / Y. Sato et awl. : J. Org. Chem. 22, 1496 (1558)7.
From the middle of the sixties besides sapogenines end steroidal alkaloids, sterols of plant origin, especially sitosterol, have been used as starting materials in the synthesis of drugs, owing to their lower price and easier availability.
It is known that 3-oxo-23,24-dinor-4,17(20)-choladien -22-oic acid (U. S. Patent specification No. 3,994,933) and 9α-hydroxy-3-oxo-23,24-dinor-4,17(20)-choladien-22-oic acid (British Patent specification No. 2,098,218) are prepared by the partial microbial side-chain degradation of sterols of animal or plant origin.
Intermediates of a pregnane skeleton were attempted to be prepared from sterol degradation products having a 23,24-dinor-cholane skeleton for the synthesis of corticosteroids.
We found that 3-oxo-23,24-dinor-4,17(20)-choladien -22-cic acid could be transformed by hydrogen peroxide into 4,16-pregnadiene-3,20-dione and 9α-hydroxy-3-oxo- -23,24-dinor-4,17(20)-choladien-22-oic acid into the novel compound 9α-hydroxy-4,16-pregnadiene-3,20-dione with a hich yield in neutral or alkaline aqueous medium, in the presence cf the salts of oxo acids of transition metals (tunsten, molybdenum, vanadium, titanium) This type of transforration has not been found in tne literature.It enables to prepare 16-dehydro-20-ox3- -pregnane derivatives which can be advantageously applied as intermediates in the synthesis of corticostercids from natural sterols, with high yield.
Firstly 3-oxo-23,24-dinor-4,9(11),17(20)-cholatrien- -22-oic acid not described in the literature was prepared from the known 9 9α-hydroxy-3-oxo-23,24-dinor-4,17(20)- -choladien-22-oic acid, by eliminating the 9 -hydroxy group. The presence of the 9(11)-double bond in this molecule opens the way for the formation of 11 llp-hydroxy or ll-oxo group, characteristic features of corticosteroid drugs, or for the introduction of a halogen substituent into position C-9, known to increase the antiinflammatcry effect Gf corticosteroids.
By microbial dehydrogenation with Arthrobacter simplex a double bond was also introduced into 3-oxo -23.24-dinor-4,9(11),17(20)-cholatrien-22-oic acid in position 1, frequently occurring in corticosteroids.
The new method suitable for the conversion of 23,24-dinorcholanoic acids unsaturated at position 17(20) to compounds with a pregnane skeleton was applied for preparing the known 4,9(11),16-pregnatriene-3,20-dione / S. BernsTein: J. Am. Chem. Soc. 81, 4956 (1955)? from 3-oxo-23,24-dinor-4,9(11),17(20)-cholatrien-22-oic acid and the new intermediate of corticosteroid synthesis, 1,4,9(11),16-Frenatetraene-3,20-dione from 3-oxo-23,24 -dinor-1,4,9(11),17(20)-cholatetraen-22-oic acid.
It may be advantageous to protect the 3-oxc group ouring the transformation of 16-dehydro-20-oxo-pregnane derivatives, prepared from 3-oxo-23, 24-dinor-4 ,17(20)- -choladien-22-oic acid or from its derivatives substituted at the sterane-skeletoR to corticosteroids. Namely, in this case an epoxide can be selectively formed at the 16 double bond and the 16cL,l7-oxydo derivative-is easily transformed into the 17 α-hydroxy compound having the characteristic 17oL-hydroxy feature of corticosteroids / P. L. Julian et al.: J. Am. Chem. Soc. 72, 5145 (1950)7.Thus the 3-oxo group of the steroids having a 3-oxo-23,24-dinor-4,17(20)choladien-22-oic acid structure is protected by blocking groups (oximes, keels, enol-ethers) and the derivatives obtained are converted into the respective 16-dehydro-20- -oxopregnane derivatives.
Based on the above the invention relates to a process for preparing 16-dehydro-20-oxcpregnane derivatives, in which a steroid having 17(20)-dehydro-23,24-dinorcholan -22-oic acid structure or a salt thereof is reacted with hydrogen peroxide in neutral or alkaline medium in the presence of salts of oxo-acids of transition metals as catalyst.
In the process of the invention any steroid having the 23,24-dinorcholan-22-oic acid sttucture with a double bond in the 17(20)-position may serve as starting material.
To prepare intermediates of 16-dehydro-2G-oxopregnane structure which are most advantageous for the synthesis of natural corticosteroid hormones and drugs it is preferred to modify the l7(20)-dehydro-23,24-dinorcholane skeleton, before applying it in the process of the invention, in a way which promotes the introduction of the 17 S and 21- -hydroxy groups characteristic for corticosteroids.
Economically it is advantageous to develop a synthesis strategy wherein the characteristic structural elements of the designed corticosteroid are already introduced to the skeleton of dinorcholanoic acids which can be inexpensively prepared by a microbial process.
According to a preferred embodiment of the present invention at first 20 to 50 mole . amounts of the ammonium or alkali metal salt of the transition metal oxo-acids, used as catalyst, are added to the aqueous solution of a salt formed, if desired, in situ with an inorganic or organic base, preferably with sodium hydroxide, of 17(20) -behydro-23,24-dinorcholanoic acid, used as starting material, then a 30 z aqueous hydrogen peroxide solution is added to the reaction mixture in 5 to 50 fold molar e-xcess.
It is preferred to apply ammonium paramolybdenate or sodium tungstate as catalyst.
Unlike the dinorcholanoic acid salts, used as starting materials, the products having a 16-detydro-20-oxopregnane skeleton are practically insaluble in water and are precIpItated from the reaction mixture.
The products can be advantageously purified by recrystallization. The con-stituents of the mother liquor can be purified by column and by preparative thin-layer chromatography.
The structure of the products prepared by the process of the invention was confirmed by ultraviolet, infrared, 1H-NMR and mass spectroscopic methods.
The process of the invention is further illustrated by the following non-limiting Examples.
Example 1 Preparation of 9α-hydroxy-4,16-pregnadiene-3,20- -dione from 9α-hydroxy-3-oxo-23,24-dinor-4,17(20)- -choladien-22-oic acid 1076 mg (3 mmoles) of 9α-hydroxy-3-oxo-23,24-dinor- -4,17(20)-choladien-22-oic acid are dissolved in a mixture of 30 ml of water and 3 ml of 1 N sodium hydroxide. Then 531 mg of ammonium paramolybdenate tetrahydrate are dissolved in 6 ml of water, the pH is adjusted to 8.0 with concentrated ammonium hydroxide, and the two solutions are combined To this reaction mixture, having pH 8.5, 20 ml of 25 % hydrogen peroxide are added dropwise at room terrperature within 2.5 hours.The foaming of the reaction mixture is reduced by the addit-ion of a few drops of dichlorcmethane.
After the reaction is completed the mixture is kept at 45 C for 30 minutes to remove the dichloromethane, then it is cooled to room temperature. The precipitated product is filtered and dried. Yield: 650 mg of 9 aS-hydroxy-4,16- -pregnadien-3,20-dione which are recrystallized first from acetone and subsequently from benzene.
M. p. : 186 to 192 cc Ultraviolet spectrum (ethanol): #max 242 nm ( & 25,400) Infrared spectral data (KBr): OH 3530, #C=O 1665, 1650, C=C 1610, 1585 cm-1 H-NMR spectral data (COCl3, #, TMS): 6.75 m (H-16), 5.90 s (H-4), 2.25 s (3H-21), 1.30 s (3H-19), 0.95 s (3H-18) ppm.
Analysis: Calculated Found C 76.79 % 76.67 % H 8.59 % 8.54 % ExamPle 2 Preparation of 4.9(11).16-pregnatriene-3,20-dione from 3-oxo-23,24-dinor-4.9(11),17(20)-cholatrien- -22-oic acid 1703 mg (5 mmoles) of 3-oxo-23,24-dinor-4,9(11),17(20)- -chclatrien-22-oic acid are dissolved in a mixture of 50 ml of water and 5 ml of 1 N sodium hydroxide. Then 8E5 mg of ammonium paramolybdenate tetrahydrate are dissolved in 15 ml of water, the pH is adjusted to 7.5 with concentrated ammonium hydroxide, and the two solutions are combined. To this reaction mixture, having pH 8.0, 15 ml of a 25 % aqueous hydrogen peroxide- solution (110 mmoles) are added dropwise at room temperature within 2 hours.The product starts to precipitate during this period, it is filtered and dried subsequently. Yield: 975 mg of crude 4,9(11),16- -pregnatriene-3,20-dione which are recrystallized first from acetone and then from methanol.
M. p.: 202 to 205 C.
[α]D = +237 (c=l, chloroform) Preparation of the starting material 3-oxo-23,24 -dinor-4,9(11),17(20)-cholatrien-22-oic acid: 1752 mg (5 mmoles) of 9 eL-hydroxy-3-oxo-23,24-dinor- 4,17(20)-choladien-22-oic acid are dissolved in 18 ml of dichloromethane, then 1.08 ml of chlorosulfonic acid are added dropwise to the stirred solution at -5 C and stirring is continued at this temperature for 30 minutes.
Thereafter 22 ml of water are added under stirring to the reaction mixture and the layers formed are separated. The aqueous layer is extracted twice with 10 ml of dichloromethane. The dichlcromethane extracts are combined and evaporated at reduced pressure. The residue, 1.84 g, is purified t) preparative thnn-layer chromatography (developing solvent: benzene-acetone 8:2). The obtained chromatographical- ly pure product is dissolved in 30 ml of methanol and 2 ml of 10 % methanolic hydrochloric acid are added. This acidic methanolic solution is diluted with 120 ml cf ethyl acetate ard the mixture is twice washed with 25 ml of water, the.
it is evaporated at reduced pressure. Yield: 1.44 g of 3-oxo -23,24-dinor-4,9(11),17(20)-cholatrien-22-oic acid which are recrystallized from methanol.
M. p. : 238 to 239 C Ultraviolet spectrum (ethanol): # max 236 nm max Infrared spectral data (KBr): 9 OH 3400-2400, 9 C=C 1675 (COOH and 3-keto), 2 C=C 1610 cm H-NMR spectral data (COCl3, #, TMS): 5.70 s (H-4), 5.50 m (H-ll), 2.00 s (3 H-21), 1.30 s (3 H-19), 0.90 s (3 H-1B) ppm Mass spectral data: M+ 340, m/z: 340, 325, 307, 295, 227, 213, 105, 91.
Example 3 Preparation of 4116-preonadiene-3,20-dione from 3-oxo-23,24-dinor-4.17(20)-choladien-22-oic acid 1028 mg (3 mmoles) of 3-oxo-23,24-dinor-4,17(20)choladien-22-oic acid are dissolved in a mixture of 30 ml of water and 3 ml of 1 N sodium hydroxide. To this solution 160 mg (0.5 mmole) of sodium tungstate dihydrate are added in 1 ml of water. Then 4.0 ml of a 30 % aqueous hydrogen peroxide solution are added dropwise to the reaction mixture at 60 C under stirring within one hour Heavy foaming is reduced by the addition of a few drops of methyl-ethyl -ketone. Thereafter the mixture is kept overnight at 5 OC.
The precipitate is filtered and dried. Yield: 623 mg of 4,16-pregnadiene-3,20-dione which are recrystallized from acetone for analytical purposes.
M. p.: 186 to 189 C [α]D = +1750 (c=0.9, chloroform) Ultraviolet spectrum (ethanol): # max 241 nm ( = 28,100) Infrared spectrum (KBr): 9 C=C 1650, 1680, 9 C=C 1585, 1615 cm~l.
Analysis: Calculated Found C 80.72 % 80.90 % H 9.03 % 9.12 % Example 4 Preparation of 1,4,9(11),16-pregnatetraene-3,20-dione from 3-oxo-23,24-dinor-1,4,9(11),17(20)-cholatetraen- -22-oic acid 1015 mg (3 mmoles) of 3-oxo-23,24-dinor-1,4,9(11),17(20)- -cholatetraen-22-oic acid are dissolved in a mixture of 30 ml cf water and 3 ml of 1 N sodium hydroxide. To this solution 160 mg (0.5 mmole) of sodium tungstate dihydrate are added in 1 ml of water. Then 3.2 ml of a 25 % aqueous hydrogen peroxide solution are added dropwise to the reaction mixture within 1.5 hours, at 60 OC under constant stirring.
Thereafter the reaction mixture is cooled to room temperature, the precipitate formed is filtered and dried. 534 g of crude 1,4,9(11),16-pregnatetraene-3,20-dione are obtained which are purified at first by preparative thin-layer chromatography (adsorbent: 2:1 mixture of Kieselgel G and Kieselgel 60 HF254+366, REANAL, Budapest; developing solvent: 4:1 mixture of benzene and acetone), then by two repeated recrystallizations from benzene.
M. p.: 204 to 208 C Ultraviolet spectrum (ethanol): k max 240 nm (= 24,300) Infrared spectral data (KBr): #C=O 1670, 1660, PC=C 1630, 1610, 1590 cm1 H-NMR spectral data (COCl3, THS): 7.20 (lH-l), 6.30 (1H-2), 6.05 (1H-4), AMXm (J1,2 = 12 Hz, 32,4 2 2 Hz, J1,4 = Hz), 6.70 t (1H16), 5.60 m (lH-1l), 2.30 s (3H-21), 1.45 s (3H-19), 0.95 s (3H-18) ppm Mass spectral data: M 308 m/z: 308, 293, 265, 172, 157, 121, 91, 43.
Analysis: Calculated Found C 81.78 8 81.70 % H 7.84 a 7.74 % Preparation of the starting material 3-oxo-23,24 -dinor-1,4,9(11),17(20)-cholatetraen-22-oic acid 10 ml portions of an Arthrobacter simplex culture induced by 4-androstene-3,17-dione for the formation of the 3-oxo-steroid-l-dehydrogenase enzyme are measured at sterile conditions into ten 500 ml Erlenmeyer flasks. Then each culture is diluted with 90 ml of 1 % (w/w) aqueous p-cyclodextrine solution. Thereafter 100 mg of 3-oxo-23,24 -dinor-4,5(1l),17(20)-cholatrien-22-oic acid, dissolved in 2 ml of ethanol, are added to each culture which are shaken on a rotary shaker at 37 OC for two days.Then the broths are combined, their pH is adjusted to 2 with 2N sulfuric acid and the fermentation broth is extracted three times with 200 ml of ethyl acetate. The ethyl acetate extracts are combined and evaporated at reduced pressure. The 960 mg of crude product obtained are purified by preparative thin -layer chromatography in a developing solvent of benzene -acetone (8:2). The 710 mg of chromatographically pure product are dissolved in 20 ml of methanol, then 1 ml of 10 0 hydrochloric acid is added in methanol at O OC. Thereafter the acidic solution is diluted with 100 ml of ethyl acetate, washed twice with 25 ml of water and evaporated at reduced pressure. The product obtained, 3-oxo-23,24 -dinor-1,4,9(11),17(20)-cholatetraen-22-oic acid, is recrystallized from methanol.
M. p. : 240 to 244 cc Ultraviolet spectrum (ethanol): #max 235 nm Infrared spectral data (KBr): # OH 3600-2500, # C=C 1690 (COOH), 1665 (3-keto), # C=C 1630, 1610 cm 1 H-NMR spectral data (CDCl3, C, TMS): 7.20 (H-l), 6.30 (H-2), 6.10 (H-4), 4Xm (J1,2 = iO Hz, 2,4 = 2 Hz, J1,4 ~ O Hz), 5.50 m (H-ll), 1.95 s (3H-2i), 1.40 s (3H-19), 0.95 s (3H-18) pD Mass spectral data: M + 338 m/z: 338, 323, 310, 211, 165, 145, 121, 91.
Example 5 Preparation of 3-methoxyimino-4,9(11),16-pregnatrien- -20-one fron 3-methoxyimino-23,24-dinor-4,9(11j,17(20 -cholatrien-22-oic acid 1109 mg (3 mmoles) of 3-methoxyimino-23,24-dinor -4,9(11),17(20)-cholatrien-22-oic acid are dissolved in a mixture of 25 ml of water, 3 ml of 1 N sodium hydroxide and 3 ml of dichloromethane. To this solution firstly 500 mg (1.5 mmoles) of sodium tungstate dihydrate are added in 3 ml water. Then 5.5 ml of a 25 % aqueous hydrogen peroxide solution are added dropwise to the reaction mixture within two hours at 55 cc under constant stirring. During the addition the product is precipitated at heavy foaming.
The precipitate formed is filtered and dried. Yield: 967 mg of 3-methoxyimino-4,9(11),16-pregnatrien-20-one, a mixture of E and Z isomers.
Infrared spectral data (KBr): ; C=O 1659, 9 C=C 1620 cm Analysis: Calculated Found C 77.84 % 77.61 % H 8.61 % 8.59 % N 4.13 % 4.53 % Preparation of the starting material 3-methoxyimino -23,24-dinor-4,9(11) ,17(20)-cholatrien-22-oic acid 2.04 g (6 mmoles) of 3-oxo-23,24-dinor-4,9(11),17(20)- -chblatrien-22-oic acid and 0.55 g (6.3 mmoles) of methoxy gamine hydrochloride are stirred in 15 ml of pyridine at rcom temperature for 20 hours. Then the reaction mixtu-re is poured to 60 ml of water and extracted three times with 20 ml of dichloromethane. The combined extracts are evaporated at reduced pressure and traces of residual pyridine are removed by azeotropic distillation first by with toluene, then with methanol. Yield: 2.13 g of 3-methoxyimino-23,24 -dinor-4,9(11),17(20)-cholatrien-22-oic acid.
Example 6 5,9(11) ,16-Preqnatriene-3,20-dion-3-ethylene ketal 1.920 mg (5 mmoles) of 3-oxo-23,24-dinor-chola -5,9(11),17(20)-trien-22-oic acid 3-ethylene ketal are dissolved in a mixture of 8.6 ml of methanol, 4.3 ml of methylethylketone, 5.2 ml of 10 M sodium hydroxide and 86 ml of water. To this solution 0.21 g of sodium tungstate dihydrate in 4.3 ml of water are added. Then 12.9 ml of a 30 % aqueous hydrogen peroxide solution are added dropwise within one hour at 60 C under constant stirring.
Thereafter the reaction mixture is heated to 85 C to remove organic solvents, then it is slowly cooled and left to stand at 5 cc overnight. Yield: 1.01 g (60 %) of 5,9(11),16-pregnatriene-3,20-dione-3-ethylene ketal which is recrystallized from methanol for analytical purposes.
M. p.: 210 to 214 C Ultraviolet spectrum (ethanol): # max 239 nm ( E.= 9600) Infrared spectral data (KBr): 9 C=O 1657, # c=c 1589 cm 1 1H-NMR spectral data (CDC13, #, TMS): 5.45 m (1H-6, lH-il), 6.70 t (lH-16), 2.25 5 (3H-21), 1.20 s (3H-19), 0.80 (3H-18) Analysis:Calculated Found C 77.92 % 77.66 % H 8.59 % 8.29 % Preparation of the starting material 3-oxo-23,24 -dinor-5,9(11),17(20)-cholatrien-22-oic acid 3-ethylene ketal 1.022 mg (3 mmoles) of 3-oxo-23,24-dinor-4,9(11),17(20)- -cholatrien-22-oic acid are dissolved in 10 ml of dichloromethane, then 20 mg of p-toluenesulfonic acid, 1 ml of ethyleneglycol, and 0.5 ml of trimethylorthoformiate are added. The reaction mixture is stirred at room temperature for 4 hours, then it is heated at 40 C until it is concentrated to half its volume. After the addition of one drop of triethylamine the solution is diluted with dichloro methane, washed with sodium hydrogen carbonate solution and water, dried over sodium sulfate and evaporated at reduced pressure. The product obtained in near quantitative yield is recrystallized from methanol for analytical purposes.
M. p.: 240 to 243 C Infrared spectral data (KBr): 9 = 1701 cm 1 1H-NMR spectral data (CDCl3, g TMS): 11.05 (1H, -COOH), 5.45 m (1H-6, H-ll), 3.95 s (4H-dioxolane), 1.95 s (3H-21), 1.20 s (3H-19), 0.90 s (3H-18) ppm Analysis Calculated Found C 74.97 % 75.02 % H 8.39 % 8.45 %

Claims (15)

CLAMS
1. A process for preparing 16-dehydro-20-oxopregnane derivatives, which co mpri ses reacting a steroid having 17(20)-dehydro-23,24-dinorcholan-22-oic acid structure or a salt therecf with hydrogen peroxide in neutral or alkaline medium in the presence of salts of oxoacids of transition metals as catalysts.
2. A process as claimed in claim 1, which r r;p r i s e s using 3-oxo-23,24-dinor-4,17(20)-choladier,- -22-cic acid as starting material.
3. A process as claimed in claim 1, which c o m pr i s e s using 9 i-hydroxy-3-cxo-23,24-cinrr-4,17(20,-choiadien-22- -oic acid as starting material.
4. A process as claimed in claim 1, which comprises using 3-oxo-23,24-dinor-4,9(11),17(20) -cholatrien-22-oic acid as starting material.
5. A process as claimed in claim 1, which comprises using 3-oxo-23,24-dinor-1,4,9(11),17(20) -cholatetraen-22-oic acid as starting material.
6. A process as claimed in claim 1, which comprises using 3-methoxyimino-23 ,24-dinor -4,9(11),17(20)-cholatrien-22-oic acid as starting material.
1. A process as claimed in claim 1, which comprises using 3-oxo-23,24-dinor-5,9(11),17(20)- -cholatrien-22-oic acid 3-ethylene ketal as starting material.
8. A process as claimed in claim 1, which comprises applying ammonium paramolybdenate tetra hydrate as catalyst.
9. A process as claimed in claim 1, which compr ise s applying sodium tungstate dihydrate as catalyst.
10. 9s -Hydroxy-4,16-pregnadiene-3,20-dione.
11. 1,4,9(11),16-Pregnatetraene-3,20-dione.
12. 3-Methoxyimino-4,9(11),16-pregnatrien-20-one.
13. 5,9(t1),16-Pregnatriene-3,20-dione 3-ethylene ketal.
14. k process as claimed in claim 1, substantially as hereinbefore described in any one of Examples 1 to 6.
15. 16-dehydro-20 oxopregnane derivatives made by the process defined in any one of claims 1 to 9 or 14.
GB8730269A 1986-12-29 1987-12-29 Process for preparing 16-dehydro-20-oxopregnane derivatives and such derivatives Expired - Lifetime GB2199325B (en)

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HU549986A HU198736B (en) 1986-12-29 1986-12-29 Process for producing 16-dehydro-20-oxopregnane derivatives

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GB2199325A true GB2199325A (en) 1988-07-06
GB2199325B GB2199325B (en) 1990-04-25

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0685487A1 (en) * 1994-06-02 1995-12-06 Roussel-Uclaf A new method of preparation of a 16-beta-steroid and new intermediates of said preparation
CN1907998B (en) * 2005-08-05 2010-05-26 天津天药药业股份有限公司 Refining process of intermediate 21-methyltetraene of dexamethasone series products
US8017793B2 (en) 2004-05-14 2011-09-13 Shionogi & Co., Ltd. Oxidation of alcohol with use of hydrogen peroxide and tungsten catalyst
CN105622700A (en) * 2016-01-29 2016-06-01 山东赛托生物科技股份有限公司 Method for synthesizing steroid intermediate 17-Acetyl-10, 13-dimethyl-6, 7, 8, 10, 12, 13, 14, 15octahydro-cyclopenta[a]phenathren-3

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4189430A (en) * 1978-07-31 1980-02-19 The Upjohn Company Epoxide process
DE2966511D1 (en) * 1978-07-31 1984-02-02 Upjohn Co Epoxypregnadienes, hydroxypregnatrienes, process for their preparation and process for their conversion to precursors of useful anti-inflammatory agents

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0685487A1 (en) * 1994-06-02 1995-12-06 Roussel-Uclaf A new method of preparation of a 16-beta-steroid and new intermediates of said preparation
FR2720747A1 (en) * 1994-06-02 1995-12-08 Roussel Uclaf Novel process for the preparation of a 16beta-methyl steroid and novel intermediates
US5596108A (en) * 1994-06-02 1997-01-21 Roussel Uclaf Process for the preparation of 16β-methylsteroids
AU689564B2 (en) * 1994-06-02 1998-04-02 Aventis Pharma S.A. New preparation process for a 16B-methyl steroid and new intermediates
US6117994A (en) * 1994-06-02 2000-09-12 Hoechst Marion Roussel Intermediates for 16β-methyl steroids
CN1057303C (en) * 1994-06-02 2000-10-11 鲁索-艾克勒夫公司 Process and intermediates for preparing 16β-methyl steroids
US8017793B2 (en) 2004-05-14 2011-09-13 Shionogi & Co., Ltd. Oxidation of alcohol with use of hydrogen peroxide and tungsten catalyst
CN1907998B (en) * 2005-08-05 2010-05-26 天津天药药业股份有限公司 Refining process of intermediate 21-methyltetraene of dexamethasone series products
CN105622700A (en) * 2016-01-29 2016-06-01 山东赛托生物科技股份有限公司 Method for synthesizing steroid intermediate 17-Acetyl-10, 13-dimethyl-6, 7, 8, 10, 12, 13, 14, 15octahydro-cyclopenta[a]phenathren-3

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HUT46035A (en) 1988-09-28
NL8703151A (en) 1988-07-18
GB2199325B (en) 1990-04-25
JPH0830076B2 (en) 1996-03-27

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