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GB1469358A - Enzyme product - Google Patents

Enzyme product

Info

Publication number
GB1469358A
GB1469358A GB2519475A GB2519475A GB1469358A GB 1469358 A GB1469358 A GB 1469358A GB 2519475 A GB2519475 A GB 2519475A GB 2519475 A GB2519475 A GB 2519475A GB 1469358 A GB1469358 A GB 1469358A
Authority
GB
United Kingdom
Prior art keywords
enzyme
reacted
copolymer
groups
acid groups
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB2519475A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nestle SA
Original Assignee
Nestle SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nestle SA filed Critical Nestle SA
Priority to GB2519475A priority Critical patent/GB1469358A/en
Publication of GB1469358A publication Critical patent/GB1469358A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F8/00—Chemical modification by after-treatment
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/087—Acrylic polymers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

1469358 Immobilized enzymes NESTLE SA 12 June 1975 25194/75 Heading C3H An enzyme is reacted with a polymer which carries both free aldehyde and free acid groups, the latter making up at least 5% of the total of such groups. The preferred enzyme is trypsin and it may be reacted with a copolymer of acrolein and acrylic acid. Sufficient free acid groups ensure that the solubility is pH dependent. Fixation of the enzyme to the copolymer is advantageously carried out in a buffer solution at room temperature. Using a buffer of pH 8 in aqueous homogeneous phase, the product is isolated by precipitation at pH 4.
GB2519475A 1975-06-12 1975-06-12 Enzyme product Expired GB1469358A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB2519475A GB1469358A (en) 1975-06-12 1975-06-12 Enzyme product

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB2519475A GB1469358A (en) 1975-06-12 1975-06-12 Enzyme product

Publications (1)

Publication Number Publication Date
GB1469358A true GB1469358A (en) 1977-04-06

Family

ID=10223753

Family Applications (1)

Application Number Title Priority Date Filing Date
GB2519475A Expired GB1469358A (en) 1975-06-12 1975-06-12 Enzyme product

Country Status (1)

Country Link
GB (1) GB1469358A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0253254A3 (en) * 1986-07-09 1988-04-27 Behringwerke Aktiengesellschaft Dispersion polymers, biologically active dispersion polymers, their preparation process and their use as a diagnostic mean
EP0246446A3 (en) * 1986-04-18 1988-05-04 Behringwerke Aktiengesellschaft Dispersion polymers, their preparation process and use
EP0707064A3 (en) * 1994-10-05 1997-07-30 Avl Medical Instr Ag Method for immobilizing biological agents in a polymer matrix as well as biosensors using such immobilizers

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0246446A3 (en) * 1986-04-18 1988-05-04 Behringwerke Aktiengesellschaft Dispersion polymers, their preparation process and use
US4962046A (en) * 1986-04-18 1990-10-09 Behringwerke Aktiengesellschaft Dispersion polymers, and their use
EP0253254A3 (en) * 1986-07-09 1988-04-27 Behringwerke Aktiengesellschaft Dispersion polymers, biologically active dispersion polymers, their preparation process and their use as a diagnostic mean
US4921915A (en) * 1986-07-09 1990-05-01 Behringwerke Aktiengesellschaft Dispersion polymers, biologically active dispersion polymers, a process for their preparation, and their use as diagnostic aids
EP0707064A3 (en) * 1994-10-05 1997-07-30 Avl Medical Instr Ag Method for immobilizing biological agents in a polymer matrix as well as biosensors using such immobilizers

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Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PE20 Patent expired after termination of 20 years

Effective date: 19950611