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GB1378134A - Process for the purification of carcinoembryonic antigen - Google Patents

Process for the purification of carcinoembryonic antigen

Info

Publication number
GB1378134A
GB1378134A GB2261173A GB2261173A GB1378134A GB 1378134 A GB1378134 A GB 1378134A GB 2261173 A GB2261173 A GB 2261173A GB 2261173 A GB2261173 A GB 2261173A GB 1378134 A GB1378134 A GB 1378134A
Authority
GB
United Kingdom
Prior art keywords
particle size
microgranular
meg
cellulose
capacity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB2261173A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US252700A external-priority patent/US3867363A/en
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of GB1378134A publication Critical patent/GB1378134A/en
Expired legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J47/00—Ion-exchange processes in general; Apparatus therefor
    • B01J47/02—Column or bed processes
    • B01J47/04—Mixed-bed processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
    • B01J39/26—Cation exchangers for chromatographic processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J47/00—Ion-exchange processes in general; Apparatus therefor
    • B01J47/014—Ion-exchange processes in general; Apparatus therefor in which the adsorbent properties of the ion-exchanger are involved, e.g. recovery of proteins or other high-molecular compounds
    • G01N33/57565—
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

1378134 Antigen purification F HOFFMANN-LA ROCHE & CO AG 11 May 1973 [12 May 1972] 22611/73 Heading A5B Human carcino-embryonic antigen is purified by (a) absorbing it on a mixed bed cellulose ion-exchange column comprising (i) microgranular diethylaminoethyl cellulose in the free base form having rod-shaped particles, a capacity of 1.0+0.1 meg/gm, a particle size distribution of 20Á to 60Á and a water regain of 2.3 to 2.8 gm/gm dry exchanger and (ii) a microgranular carboxymethyl cellulose with a capacity of 1.0 Œ 0.1 meg/gm, having rod-shaped particles, a particle size distribution of 20Á to 60Á and a water regain of 2.3 to 2.7 gm/gm dry exchanger, (b) eluting the column with an aqueous solution of ammonium acetate and sodium chloride, (c) subjecting the eluate to sequential chromatography first on an agarose gel containing about 6% agarose and having a particle size of 40 to 210Á and then on a hydrophilic water-insoluble cross-linked dextran polymer gel and (d) collecting and concentrating the eluate fractions which have a spectrophotometric absorption peak at 280 mÁ and a molecular weight of 200,000 to 500,000.
GB2261173A 1972-05-12 1973-05-11 Process for the purification of carcinoembryonic antigen Expired GB1378134A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US252700A US3867363A (en) 1970-06-01 1972-05-12 Carcinoembryonic antigens

Publications (1)

Publication Number Publication Date
GB1378134A true GB1378134A (en) 1974-12-18

Family

ID=22957148

Family Applications (1)

Application Number Title Priority Date Filing Date
GB2261173A Expired GB1378134A (en) 1972-05-12 1973-05-11 Process for the purification of carcinoembryonic antigen

Country Status (15)

Country Link
JP (1) JPS5912990B2 (en)
AU (1) AU468779B2 (en)
BE (1) BE799405A (en)
CA (1) CA948991A (en)
CH (1) CH583036A5 (en)
DD (1) DD107293A5 (en)
DE (1) DE2323955A1 (en)
DK (1) DK133559B (en)
FR (1) FR2184753B1 (en)
GB (1) GB1378134A (en)
IL (1) IL42119A (en)
IT (1) IT998101B (en)
NL (1) NL7306346A (en)
SE (1) SE403912B (en)
ZA (1) ZA732770B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4146603A (en) * 1977-02-18 1979-03-27 Research Corporation Tumor specific glycoproteins and method for detecting tumorigenic cancers
CA1225930A (en) * 1982-06-07 1987-08-25 Otto A. Gansow Metal chelate conjugated monoclonal antibodies
JPS6076395A (en) * 1983-10-03 1985-04-30 Dainippon Screen Mfg Co Ltd Eluting device for printing plate

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3697638A (en) * 1970-06-01 1972-10-10 Hoffmann La Roche Antigens

Also Published As

Publication number Publication date
FR2184753A1 (en) 1973-12-28
CH583036A5 (en) 1976-12-31
AU468779B2 (en) 1976-01-22
FR2184753B1 (en) 1977-11-04
IL42119A0 (en) 1973-06-29
NL7306346A (en) 1973-11-14
CA948991A (en) 1974-06-11
BE799405A (en) 1973-11-12
DE2323955A1 (en) 1973-11-22
IL42119A (en) 1976-08-31
DK133559C (en) 1977-02-21
IT998101B (en) 1976-01-20
DD107293A5 (en) 1974-07-20
JPS4947521A (en) 1974-05-08
DK133559B (en) 1976-06-08
AU5498973A (en) 1974-10-31
ZA732770B (en) 1974-04-24
SE403912B (en) 1978-09-11
JPS5912990B2 (en) 1984-03-27

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Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PCNP Patent ceased through non-payment of renewal fee