FR8304M - - Google Patents
Info
- Publication number
- FR8304M FR8304M FR168937A FR168937A FR8304M FR 8304 M FR8304 M FR 8304M FR 168937 A FR168937 A FR 168937A FR 168937 A FR168937 A FR 168937A FR 8304 M FR8304 M FR 8304M
- Authority
- FR
- France
- Prior art keywords
- extraction
- component
- acetic acid
- product
- thyrocalcitonin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 abstract 6
- 238000000605 extraction Methods 0.000 abstract 6
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 abstract 4
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 abstract 4
- 238000000034 method Methods 0.000 abstract 4
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 abstract 2
- 239000005695 Ammonium acetate Substances 0.000 abstract 2
- 108060001064 Calcitonin Proteins 0.000 abstract 2
- 229940043376 ammonium acetate Drugs 0.000 abstract 2
- 235000019257 ammonium acetate Nutrition 0.000 abstract 2
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 abstract 2
- 239000002904 solvent Substances 0.000 abstract 2
- 210000001685 thyroid gland Anatomy 0.000 abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 abstract 1
- 102000055006 Calcitonin Human genes 0.000 abstract 1
- MHLMRBVCMNDOCW-UHFFFAOYSA-N acetic acid;butan-1-ol;hydrate Chemical compound O.CC(O)=O.CCCCO MHLMRBVCMNDOCW-UHFFFAOYSA-N 0.000 abstract 1
- 239000004480 active ingredient Substances 0.000 abstract 1
- 239000000287 crude extract Substances 0.000 abstract 1
- 239000000284 extract Substances 0.000 abstract 1
- 238000002523 gelfiltration Methods 0.000 abstract 1
- 210000004907 gland Anatomy 0.000 abstract 1
- 230000001184 hypocalcaemic effect Effects 0.000 abstract 1
- 239000008194 pharmaceutical composition Substances 0.000 abstract 1
- 229940045662 porcine thyroid Drugs 0.000 abstract 1
- 239000007858 starting material Substances 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/22—Hormones
- A61K38/23—Calcitonins
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/04—Solvent extraction of solutions which are liquid
- B01D11/0492—Applications, solvents used
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmacology & Pharmacy (AREA)
- Endocrinology (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
1,219,316. Thyrocalcitonin extraction. MERCK & CO Inc. 8 July, 1968 [14 July, 1967], No. 32477/68. Heading A5B. The hypocalcemic peptide thyrocalcitonin is obtained in a pure state from a thyroid gland extract by the process comprising countercurrent extraction with a two phase, five component solvent system consisting essentially of pyridine, n-butanol, acetic acid, ammonium acetate and water in the proportions of 60-100 ml. of pyridine, 100 ml. of n-butanol, 1.2 to 5 ml. of acetic acid, 100 to 300 ml. of water, and 1 to 5 grams of ammonium acetate. The product of the extraction process may be further purified by countercurrent extraction with a two phase, three component n-butanolwater-acetic acid system, alternatively, the procedure may be reversed. The 3-component system may also be used before and after treatment with the 5-component system. The product may be further purified by conventional chromatographic or gel filtration methods. The preferred starting material is porcine thyroid glands and in particular a crude extract of the defatted gland obtained by extraction with a solvent system similar or the same as that used for the countercurrent extraction process. Reference is also made to pharmaceutical compositions containing as the active ingredient the product of the process of the invention.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US65334267A | 1967-07-14 | 1967-07-14 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| FR8304M true FR8304M (en) | 1970-11-23 |
Family
ID=24620462
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FR1589679D Expired FR1589679A (en) | 1967-07-14 | 1968-07-08 | |
| FR168937A Expired FR8304M (en) | 1967-07-14 | 1968-10-07 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FR1589679D Expired FR1589679A (en) | 1967-07-14 | 1968-07-08 |
Country Status (3)
| Country | Link |
|---|---|
| DE (1) | DE1792012A1 (en) |
| FR (2) | FR1589679A (en) |
| GB (1) | GB1219316A (en) |
-
1968
- 1968-07-08 GB GB3247768A patent/GB1219316A/en not_active Expired
- 1968-07-08 FR FR1589679D patent/FR1589679A/fr not_active Expired
- 1968-07-11 DE DE19681792012 patent/DE1792012A1/en active Pending
- 1968-10-07 FR FR168937A patent/FR8304M/fr not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| GB1219316A (en) | 1971-01-13 |
| FR1589679A (en) | 1970-04-06 |
| DE1792012A1 (en) | 1971-10-21 |
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