FI93734B - Förfarande för framställning av fusionsproteiner som innehåller en eukaryot ballastandel och produkter som är användbara vid förfarandet - Google Patents
Förfarande för framställning av fusionsproteiner som innehåller en eukaryot ballastandel och produkter som är användbara vid förfarandet Download PDFInfo
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- FI93734B FI93734B FI865187A FI865187A FI93734B FI 93734 B FI93734 B FI 93734B FI 865187 A FI865187 A FI 865187A FI 865187 A FI865187 A FI 865187A FI 93734 B FI93734 B FI 93734B
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- plasmid
- amino acid
- fusion protein
- sequence
- acid sequence
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- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- KOSRFJWDECSPRO-UHFFFAOYSA-N alpha-L-glutamyl-L-glutamic acid Natural products OC(=O)CCC(N)C(=O)NC(CCC(O)=O)C(O)=O KOSRFJWDECSPRO-UHFFFAOYSA-N 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N aspartic acid group Chemical group N[C@@H](CC(=O)O)C(=O)O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 230000001588 bifunctional effect Effects 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 102000013165 exonuclease Human genes 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 108010055341 glutamyl-glutamic acid Proteins 0.000 description 1
- 210000003714 granulocyte Anatomy 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 239000000543 intermediate Substances 0.000 description 1
- 108010090333 leucyl-lysyl-proline Proteins 0.000 description 1
- 238000007169 ligase reaction Methods 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 108091005573 modified proteins Proteins 0.000 description 1
- 102000035118 modified proteins Human genes 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229910021420 polycrystalline silicon Inorganic materials 0.000 description 1
- 229920005591 polysilicon Polymers 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 125000001500 prolyl group Chemical group [H]N1C([H])(C(=O)[*])C([H])([H])C([H])([H])C1([H])[H] 0.000 description 1
- 230000006337 proteolytic cleavage Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000012807 shake-flask culturing Methods 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 108010051110 tyrosyl-lysine Proteins 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/53—Colony-stimulating factor [CSF]
- C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/55—IL-2
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Pharmacology & Pharmacy (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Physics & Mathematics (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Diabetes (AREA)
- Plant Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Endocrinology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Claims (9)
1. Förfarande för f raitista lining av fusionsproteiner med en C- eller N-terminal del som motsvarar en interleu-5 kin-2 (IL-2) -molekyl, företrädesvis humant IL-2, men som innehäller mindre än 100 aminosyror, med undantag av de delar som innehäller 96 - 99 aminosyror, känne-t e c k n a t därav, att en gen som kodar för fusionspro-teinet bringas till expression i en värdcell. 10
2. Förfarande enligt patentkrav 1, känne- t e c k n a t därav, att genen som kodar för IL-2-delen omfattar en del av DNA-sekvensen I (bilaga).
3. Förfarande enligt patentkrav 2, känne- t e c k n a t därav, att genen som kodar för IL-2-delen 15 bestär av ett, tvä eller tre av segmenten A - F i IL-2-genen (EcoRI)-A-Pstl-B-Mlul-C-Xbal-D-Sacl-E-Pvul-F-(Sali) i valbar ordningsföljd ooh eventuellt sammankopplade me-delst adaptor- eller kopplingssekvenser.
4. Förfarande enligt nägot av patentkraven 1-3, 20 kännetecknat därav, att mellan IL-2-sekvensen ooh det önskade proteinets aminosyrasekvens finns en ami-nosyra eller aminosyrasekvens, som möjliggör kemisk eller enzymatisk avspjälkning av det önskade proteinet frän IL-2-delen, företrädesvis aminosyran Met, Cys, Trp, Lys eller 25 Arg eller en aminosyrasekvens, som innehäller nägon (näg-ra) av dessa aminosyror i C-terminalen.
5. Förfarande enligt patentkrav 4, kännetecknat därav, att aminosyrasekvensen är Asp-Pro eller Ile-Glu-Gly-Arg eller innehäller denna sekvens i C- . 30 terminalen.
6. Förfarande enligt nägot av patentkraven 1-5, kännetecknat därav, att genen som kodar för fusionsproteinet bringas till expression i en bakterie-cell, företrädesvis i E. coli. 93734
7. Användning av fusionsprotein soin kan erhällas enligt nägot av patentkraven 1-6 för framställning av önskade proteiner.
8. Genstruktur, kännetecknad därav, 5 att den kodar för ett fusionsprotein som kan erhällas enligt nägot av patentkraven 1-6.
9. pUC-derivaten pW226 (figur C), pW227 (figur 11), pW228 (figur 12) , pH 200 och pH 201 (figur 13) ; expres-sionsplasmiderna pW226-l (figur C) , pW227-l (figur 11) , 10 pW228-l (figur 12) och pH 202 (figur 13) ; expressionsplas- mider, som kodar ett fusionsprotein innehällande en i pa-tentkrav 1 angiven IL-2-del, en bryggdel och proinsulin: pK40 (figur 1), pSL12 (figur 2), pK50 (figur 3), pK51 (figur 4), pK52 (figur 5), pK53 (figur 6), pSL14 (figur 7), 15 pPH31 (figur 8); en expressionsplasmid, som kodar ett fusionsprotein innehällande en i patentkrav 1 angiven IL-2-del, en bryggdel och hirudin: pK192 (figur 9); expres- sionsplasmider, som kodar ett fusionsprotein innehällande en i patentkrav l angiven IL-2-del, en bryggdel och GM-20 CSF: pW214 (figur 10), pW233 (figur 11), pW234 (figur 12).
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE3545565 | 1985-12-21 | ||
| DE3545565 | 1985-12-21 | ||
| DE3636903 | 1986-10-30 | ||
| DE19863636903 DE3636903A1 (de) | 1985-12-21 | 1986-10-30 | Fusionsproteine mit eukaryotischem ballastanteil |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| FI865187A0 FI865187A0 (sv) | 1986-12-18 |
| FI865187L FI865187L (sv) | 1987-06-22 |
| FI93734B true FI93734B (sv) | 1995-02-15 |
| FI93734C FI93734C (sv) | 1995-05-26 |
Family
ID=25839208
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI865187A FI93734C (sv) | 1985-12-21 | 1986-12-18 | Förfarande för framställning av fusionsproteiner som innehåller en eukaryot ballastandel och produkter som är användbara vid förfarandet |
Country Status (16)
| Country | Link |
|---|---|
| EP (1) | EP0229998B1 (sv) |
| JP (2) | JP2553058B2 (sv) |
| KR (1) | KR950000301B1 (sv) |
| AT (1) | ATE78296T1 (sv) |
| AU (1) | AU599943B2 (sv) |
| CA (1) | CA1339894C (sv) |
| DE (1) | DE3636903A1 (sv) |
| DK (1) | DK168823B1 (sv) |
| ES (1) | ES2033678T3 (sv) |
| FI (1) | FI93734C (sv) |
| GR (1) | GR3005985T3 (sv) |
| HU (1) | HU209747B (sv) |
| IE (1) | IE58935B1 (sv) |
| IL (1) | IL81018A (sv) |
| NO (1) | NO175003C (sv) |
| PT (1) | PT83973B (sv) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3545568A1 (de) * | 1985-12-21 | 1987-07-16 | Hoechst Ag | Gm-csf-protein, seine derivate, herstellung solcher proteine und ihre verwendung |
| DE3844211A1 (de) * | 1988-12-29 | 1990-07-05 | Hoechst Ag | Neue insulinderivate, verfahren zu deren herstellung, ihre verwendung und eine sie enthaltende pharmazeutische zubereitung |
| FR2643646B1 (fr) * | 1989-02-27 | 1993-09-17 | Pasteur Institut | Expression de sequences de nucleotides codant pour des vesicules a gaz |
| CU22222A1 (es) * | 1989-08-03 | 1995-01-31 | Cigb | Procedimiento para la expresion de proteinas heterologicas producidas de forma fusionada en escherichia coli, su uso, vectores de expresion y cepas recombinantes |
| DE3942580A1 (de) * | 1989-12-22 | 1991-06-27 | Basf Ag | Verfahren zur herstellung von hirudin |
| DE4105480A1 (de) * | 1991-02-21 | 1992-08-27 | Boehringer Mannheim Gmbh | Verbesserte aktivierung von rekombinanten proteinen |
| ES2218622T3 (es) | 1996-07-26 | 2004-11-16 | Aventis Pharma Deutschland Gmbh | Derivados de insulina con actividad de union al zinc incrementada. |
| DE19825447A1 (de) | 1998-06-06 | 1999-12-09 | Hoechst Marion Roussel De Gmbh | Neue Insulinanaloga mit erhöhter Zinkbildung |
| DE102006031962A1 (de) | 2006-07-11 | 2008-01-17 | Sanofi-Aventis Deutschland Gmbh | Amidiertes Insulin Glargin |
| DE102006031955A1 (de) | 2006-07-11 | 2008-01-17 | Sanofi-Aventis Deutschland Gmbh | Verfahren zur Herstellung von Insulinanaloga mit dibasischem B-Kettenende |
| DK2349324T3 (en) | 2008-10-17 | 2017-12-11 | Sanofi Aventis Deutschland | COMBINATION OF AN INSULIN AND A GLP-1 AGONIST |
| ES2965209T3 (es) | 2009-11-13 | 2024-04-11 | Sanofi Aventis Deutschland | Composición farmacéutica que comprende desPro36exendina-4(1-39)-Lys6-NH2 y metionina |
| KR101836070B1 (ko) | 2009-11-13 | 2018-03-09 | 사노피-아벤티스 도이칠란트 게엠베하 | Ｇｌｐ－１ 작용제, 인슐린 및 메티오닌을 포함하는 약제학적 조성물 |
| CN103179978A (zh) | 2010-08-30 | 2013-06-26 | 赛诺菲-安万特德国有限公司 | Ave0010用于制造供治疗2型糖尿病用的药物的用途 |
| US9821032B2 (en) | 2011-05-13 | 2017-11-21 | Sanofi-Aventis Deutschland Gmbh | Pharmaceutical combination for improving glycemic control as add-on therapy to basal insulin |
| ES2550357T3 (es) | 2011-08-29 | 2015-11-06 | Sanofi-Aventis Deutschland Gmbh | Combinación farmacéutica para su uso en el control glucémico en pacientes de diabetes de tipo 2 |
| TWI559929B (en) | 2011-09-01 | 2016-12-01 | Sanofi Aventis Deutschland | Pharmaceutical composition for use in the treatment of a neurodegenerative disease |
| HRP20230470T1 (hr) | 2014-12-12 | 2023-07-21 | Sanofi-Aventis Deutschland Gmbh | Formulacija fiksnog omjera inzulin glargin/liksisenatid |
| TWI748945B (zh) | 2015-03-13 | 2021-12-11 | 德商賽諾菲阿凡提斯德意志有限公司 | 第２型糖尿病病患治療 |
| TW201705975A (zh) | 2015-03-18 | 2017-02-16 | 賽諾菲阿凡提斯德意志有限公司 | 第２型糖尿病病患之治療 |
| KR20200080747A (ko) | 2018-12-27 | 2020-07-07 | 주식회사 폴루스 | 인슐린 전구체의 인슐린 효소 전환용 조성물 및 이를 이용하여 인슐린 전구체를 인슐린으로 전환하는 방법 |
| KR20200080748A (ko) | 2018-12-27 | 2020-07-07 | 주식회사 폴루스 | 음이온 교환 크로마토그래피를 이용한 인슐린 전구체의 정제방법 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK108685A (da) * | 1984-03-19 | 1985-09-20 | Fujisawa Pharmaceutical Co | Vaekstfaktor i |
| EP0158198A1 (en) * | 1984-03-29 | 1985-10-16 | Takeda Chemical Industries, Ltd. | DNA and use thereof |
| GB8412517D0 (en) * | 1984-05-16 | 1984-06-20 | Nagai K | Recombinant fusion proteins |
-
1986
- 1986-10-30 DE DE19863636903 patent/DE3636903A1/de not_active Withdrawn
- 1986-12-16 EP EP86117481A patent/EP0229998B1/de not_active Expired - Lifetime
- 1986-12-16 AT AT86117481T patent/ATE78296T1/de not_active IP Right Cessation
- 1986-12-16 ES ES198686117481T patent/ES2033678T3/es not_active Expired - Lifetime
- 1986-12-18 IL IL81018A patent/IL81018A/xx not_active IP Right Cessation
- 1986-12-18 FI FI865187A patent/FI93734C/sv not_active IP Right Cessation
- 1986-12-19 AU AU66760/86A patent/AU599943B2/en not_active Expired
- 1986-12-19 DK DK619186A patent/DK168823B1/da not_active IP Right Cessation
- 1986-12-19 CA CA000525858A patent/CA1339894C/en not_active Expired - Lifetime
- 1986-12-19 IE IE333486A patent/IE58935B1/en not_active IP Right Cessation
- 1986-12-19 PT PT83973A patent/PT83973B/pt unknown
- 1986-12-19 NO NO865192A patent/NO175003C/no not_active IP Right Cessation
- 1986-12-19 HU HU865354A patent/HU209747B/hu unknown
- 1986-12-20 KR KR1019860011010A patent/KR950000301B1/ko not_active Expired - Lifetime
- 1986-12-22 JP JP61306185A patent/JP2553058B2/ja not_active Expired - Lifetime
-
1992
- 1992-10-15 GR GR920402187T patent/GR3005985T3/el unknown
-
1995
- 1995-10-11 JP JP7263310A patent/JP2774260B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| DK619186A (da) | 1987-06-22 |
| IL81018A0 (en) | 1987-03-31 |
| KR870006187A (ko) | 1987-07-09 |
| IL81018A (en) | 1992-03-29 |
| NO865192L (no) | 1987-06-22 |
| JPS62167799A (ja) | 1987-07-24 |
| DK168823B1 (da) | 1994-06-20 |
| NO175003C (no) | 1994-08-17 |
| HU209747B (en) | 1994-10-28 |
| JPH08187089A (ja) | 1996-07-23 |
| FI865187L (sv) | 1987-06-22 |
| DK619186D0 (da) | 1986-12-19 |
| JP2774260B2 (ja) | 1998-07-09 |
| PT83973B (pt) | 1989-07-31 |
| IE58935B1 (en) | 1993-12-01 |
| IE863334L (en) | 1987-06-21 |
| CA1339894C (en) | 1998-06-02 |
| FI93734C (sv) | 1995-05-26 |
| AU599943B2 (en) | 1990-08-02 |
| DE3636903A1 (de) | 1987-07-02 |
| EP0229998B1 (de) | 1992-07-15 |
| GR3005985T3 (sv) | 1993-06-07 |
| HUT44614A (en) | 1988-03-28 |
| ES2033678T3 (es) | 1993-04-01 |
| ATE78296T1 (de) | 1992-08-15 |
| JP2553058B2 (ja) | 1996-11-13 |
| EP0229998A3 (en) | 1988-08-03 |
| AU6676086A (en) | 1987-06-25 |
| NO865192D0 (no) | 1986-12-19 |
| PT83973A (de) | 1987-01-01 |
| EP0229998A2 (de) | 1987-07-29 |
| NO175003B (no) | 1994-05-09 |
| FI865187A0 (sv) | 1986-12-18 |
| KR950000301B1 (ko) | 1995-01-13 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| BB | Publication of examined application | ||
| FG | Patent granted |
Owner name: HOECHST AKTIENGESELLSCHAFT |
|
| MA | Patent expired |