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ES8704540A1 - Procedimiento para preparar polipeptidos a partir de aminoa-cidos geneticamente codificables - Google Patents

Procedimiento para preparar polipeptidos a partir de aminoa-cidos geneticamente codificables

Info

Publication number
ES8704540A1
ES8704540A1 ES543120A ES543120A ES8704540A1 ES 8704540 A1 ES8704540 A1 ES 8704540A1 ES 543120 A ES543120 A ES 543120A ES 543120 A ES543120 A ES 543120A ES 8704540 A1 ES8704540 A1 ES 8704540A1
Authority
ES
Spain
Prior art keywords
isolation
sequence
pst
arg
ala
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES543120A
Other languages
English (en)
Other versions
ES543120A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hoechst AG
Original Assignee
Hoechst AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst AG filed Critical Hoechst AG
Publication of ES543120A0 publication Critical patent/ES543120A0/es
Publication of ES8704540A1 publication Critical patent/ES8704540A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Detergent Compositions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

METODO DE PREPARACION DE POLIPEPTIDOS A PARTIR DE AMINOACIDOS CODIFICABLES GENETICAMENTE. CONSISTE EN EXPRESAR EN UNA CELULA HOSPEDANTE DEL GENERO STREPTOMYCES UNA ESTRUCTURA DE GEN, QUE CONTIENE EN EL MARCO DE LECTURA CON EL GEN ESTRUCTURAL PARA EL POLIPEPTIDO A PREPARAR LA SECUENCIA DE ADN DE UN PREPEPTIDO, EL CUAL SE HALLA INMEDIATAMENTE ANTES DEL GEN ESTRUCTURAL, CODIFICA EN EL EXTREMO AMINO MET-ARG-VAL-ARG-ALA-LEN-ARG, CODIFICA EN EL EXTREMO CARBOXI ALA-SER-ALA, Y CODIFICA EN EL MEDIO DE UNA REGION HIDROFOBICA QUE ABARCA DE 10 A 25 AMINOACIDOS. TIENE APLICACIONES PARA LA PREPARACION DE PEPTIDOS QUE PRESENTAN EL RADICAL TENDAMISTATO.
ES543120A 1984-05-17 1985-05-14 Procedimiento para preparar polipeptidos a partir de aminoa-cidos geneticamente codificables Expired ES8704540A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19843418274 DE3418274A1 (de) 1984-05-17 1984-05-17 Signalpeptid fuer die exkretion von peptiden in streptomyceten

Publications (2)

Publication Number Publication Date
ES543120A0 ES543120A0 (es) 1987-04-01
ES8704540A1 true ES8704540A1 (es) 1987-04-01

Family

ID=6236073

Family Applications (1)

Application Number Title Priority Date Filing Date
ES543120A Expired ES8704540A1 (es) 1984-05-17 1985-05-14 Procedimiento para preparar polipeptidos a partir de aminoa-cidos geneticamente codificables

Country Status (17)

Country Link
EP (1) EP0161629B1 (es)
JP (1) JPH0797993B2 (es)
AT (1) ATE36167T1 (es)
AU (1) AU580062B2 (es)
CA (1) CA1309674C (es)
DE (2) DE3418274A1 (es)
DK (1) DK172458B1 (es)
ES (1) ES8704540A1 (es)
FI (1) FI81113C (es)
GR (1) GR851184B (es)
HU (1) HU197351B (es)
IE (1) IE58385B1 (es)
IL (1) IL75214A (es)
NO (1) NO173452C (es)
NZ (1) NZ212085A (es)
PT (1) PT80483B (es)
ZA (1) ZA853672B (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1295563C (en) * 1985-11-01 1992-02-11 Robert T. Garvin Production of active proteins containing cystine residues
CA1295566C (en) * 1987-07-21 1992-02-11 Robert T. Garvin Characterization and structure of genes for protease a and protease b from streptomyces griseus
DE3707150A1 (de) * 1987-03-06 1988-09-15 Hoechst Ag Tendamistat-derivate
DE3714866A1 (de) * 1987-05-05 1988-11-24 Hoechst Ag Verfahren zur herstellung von fremdproteinen in streptomyceten
US5426036A (en) * 1987-05-05 1995-06-20 Hoechst Aktiengesellschaft Processes for the preparation of foreign proteins in streptomycetes
DE4012818A1 (de) 1990-04-21 1991-10-24 Hoechst Ag Verfahren zur herstellung von fremdproteinen in streptomyceten
DE3837271A1 (de) * 1988-11-03 1990-05-10 Hoechst Ag Verfahren zur selektiven spaltung von fusionsproteinen
EP0367163B1 (de) * 1988-11-03 1996-01-03 Hoechst Aktiengesellschaft Verfahren zur Herstellung eines Insulin-Vorprodukts in Streptomyceten

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3331860A1 (de) * 1983-09-03 1985-03-21 Hoechst Ag, 6230 Frankfurt Verfahren zur herstellung von tendamistat

Also Published As

Publication number Publication date
IL75214A (en) 1991-03-10
FI81113C (fi) 1990-09-10
DK217285D0 (da) 1985-05-15
NO851971L (no) 1985-11-18
IE851225L (en) 1985-11-17
DE3564118D1 (en) 1988-09-08
DK217285A (da) 1985-11-18
GR851184B (es) 1985-11-25
EP0161629A1 (de) 1985-11-21
AU4264085A (en) 1985-11-21
FI851929A0 (fi) 1985-05-15
ZA853672B (en) 1985-12-24
HU197351B (en) 1989-03-28
FI81113B (fi) 1990-05-31
HUT38670A (en) 1986-06-30
NO173452C (no) 1993-12-22
NO173452B (no) 1993-09-06
JPH0797993B2 (ja) 1995-10-25
ES543120A0 (es) 1987-04-01
CA1309674C (en) 1992-11-03
NZ212085A (en) 1989-03-29
IL75214A0 (en) 1985-09-29
EP0161629B1 (de) 1988-08-03
ATE36167T1 (de) 1988-08-15
JPS60260598A (ja) 1985-12-23
FI851929L (fi) 1985-11-18
AU580062B2 (en) 1988-12-22
IE58385B1 (en) 1993-09-08
PT80483A (de) 1985-06-01
DK172458B1 (da) 1998-08-24
DE3418274A1 (de) 1985-11-21
PT80483B (pt) 1987-09-30

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20011110