ES8602933A1 - Un metodo para clonar por tecnicas de dna recombinante penicilinacilasas e incrementar su produccion por fermentacion - Google Patents
Un metodo para clonar por tecnicas de dna recombinante penicilinacilasas e incrementar su produccion por fermentacionInfo
- Publication number
- ES8602933A1 ES8602933A1 ES534757A ES534757A ES8602933A1 ES 8602933 A1 ES8602933 A1 ES 8602933A1 ES 534757 A ES534757 A ES 534757A ES 534757 A ES534757 A ES 534757A ES 8602933 A1 ES8602933 A1 ES 8602933A1
- Authority
- ES
- Spain
- Prior art keywords
- penicillinacylase
- recombinant dna
- gene cloning
- isolating
- dna technique
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000000034 method Methods 0.000 title abstract 2
- 108020004511 Recombinant DNA Proteins 0.000 title 1
- 238000012215 gene cloning Methods 0.000 title 1
- 108010073038 Penicillin Amidase Proteins 0.000 abstract 4
- 244000005700 microbiome Species 0.000 abstract 2
- WLJVXDMOQOGPHL-UHFFFAOYSA-N phenylacetic acid Chemical compound OC(=O)CC1=CC=CC=C1 WLJVXDMOQOGPHL-UHFFFAOYSA-N 0.000 abstract 2
- 230000000694 effects Effects 0.000 abstract 1
- ZHNUHDYFZUAESO-UHFFFAOYSA-N formamide Substances NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 230000002068 genetic effect Effects 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 238000012986 modification Methods 0.000 abstract 1
- 230000004048 modification Effects 0.000 abstract 1
- 229960003424 phenylacetic acid Drugs 0.000 abstract 1
- 239000003279 phenylacetic acid Substances 0.000 abstract 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
PROCEDIMIENTO PARA CLONAR EN MICROORGANISMOS LOS GENES QUE CODIFICAN PARA LAS PROTEINAS CON ACTIVIDAD DE PENICILINACILASA Y PRODUCIRLAS POR FERMENTACION. COMPRENDE LAS SIGUIENTES OPERACIONES: PRIMERA, SE SELECCIONAN MICROORGANISMOS PRODUCTORES DE PENICILINACILASA Y SE AISLAN DE ELLOS LOS FRAGMENTOS DE DNA QUE CONTIENEN LA INFORMACION GENETICA RELATIVA A LA PRODUCCION DE PENICILINACILASA Y SE INSERTAN EN UN VECTOR DE DNA PARA OBTENER LOS CORRESPONDIENTES VECTORES RECOMBINANTES; SEGUNDA, SE INTRODUCEN DICHOS VECTORES EN LAS CELULAS DE UN MICROORGANISMO HUESPED, NO PRODUCTOR DE PENICILINACILASA, DE CEPAS AUXOTROFAS PARA COMPUESTOS CAPACES DE ORIGINAR ENLACES AMIDA CON EL ACIDO FENILACETICO; Y POR ULTIMO, SE AISLAN LOS TRANSFORMANTES CON ACTIVIDAD DE PENICILINACILASA Y SE CULTIVAN LAS CELULAS TRANSFORMANTES EN UN MEDIO DE CULTIVO APROPIADO.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES534757A ES8602933A1 (es) | 1984-07-31 | 1984-07-31 | Un metodo para clonar por tecnicas de dna recombinante penicilinacilasas e incrementar su produccion por fermentacion |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES534757A ES8602933A1 (es) | 1984-07-31 | 1984-07-31 | Un metodo para clonar por tecnicas de dna recombinante penicilinacilasas e incrementar su produccion por fermentacion |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES534757A0 ES534757A0 (es) | 1985-12-16 |
| ES8602933A1 true ES8602933A1 (es) | 1985-12-16 |
Family
ID=8487719
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES534757A Expired ES8602933A1 (es) | 1984-07-31 | 1984-07-31 | Un metodo para clonar por tecnicas de dna recombinante penicilinacilasas e incrementar su produccion por fermentacion |
Country Status (1)
| Country | Link |
|---|---|
| ES (1) | ES8602933A1 (es) |
-
1984
- 1984-07-31 ES ES534757A patent/ES8602933A1/es not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| ES534757A0 (es) | 1985-12-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FD1A | Patent lapsed |
Effective date: 20010201 |