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ES8601303A1 - Procedimiento para preparar un polipeptido - Google Patents

Procedimiento para preparar un polipeptido

Info

Publication number
ES8601303A1
ES8601303A1 ES534589A ES534589A ES8601303A1 ES 8601303 A1 ES8601303 A1 ES 8601303A1 ES 534589 A ES534589 A ES 534589A ES 534589 A ES534589 A ES 534589A ES 8601303 A1 ES8601303 A1 ES 8601303A1
Authority
ES
Spain
Prior art keywords
amino
peptides
genetic engineering
carboxamide
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES534589A
Other languages
English (en)
Other versions
ES534589A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hoechst AG
Original Assignee
Hoechst AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst AG filed Critical Hoechst AG
Publication of ES534589A0 publication Critical patent/ES534589A0/es
Publication of ES8601303A1 publication Critical patent/ES8601303A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/60—Growth hormone-releasing factor [GH-RF], i.e. somatoliberin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Endocrinology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

METODO DE PREPARACION DE POLIPEPTIDOS DE FORMULA Y-R-NH2 EN LA QUE Y REPRESENTA EL RADICAL METIONINO O DE UNA PROTEINA BACTERIANA UNIDO A TRAVES DE METIONINA; Y R REPRESENTA UNA SECUENCIA DE PEPTIDO A BASE DE AMINOACIDOS CODIFICABLES GENETICAMENTE. COMPRENDE LA SINTESIS POR TECNOLOGIA GENETICA DE UN POLIPEPTIDO DE FORMULA Y-R-NH-CH2-COOH A TRAVES DE DOS FRAGMENTOS DE GEN QUE SON SINTETIZADOS QUIMICAMENTE A PARTIR DE ESLABONES MENORES DE UNA SOLA HELICE. AMBOS FRAGMENTOS SON UNIDOS ENZIMATICAMENTE E INCORPORADOS EN UN VECTOR APROPIADO DONDE SON AMPLIFICADOS. EL PEPTIDO AISLADO DIRECTAMENTE O COMO PROTEINA DE FUSION SE TRANSFORMA ENZIMATICAMENTE EN EL POLIPEPTIDO DESEADO. ESTOS POLIPEPTIDOS TIENEN APLICACIONES FARMACOLOGICAS POR SU CAPACIDAD PARA LIBERAR LA HORMONA DEL CRECIMIENTO.
ES534589A 1983-07-27 1984-07-24 Procedimiento para preparar un polipeptido Expired ES8601303A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19833327007 DE3327007A1 (de) 1983-07-27 1983-07-27 Herstellung von polypeptiden mit einem saeureamid-carboxyterminus

Publications (2)

Publication Number Publication Date
ES534589A0 ES534589A0 (es) 1985-10-16
ES8601303A1 true ES8601303A1 (es) 1985-10-16

Family

ID=6205008

Family Applications (1)

Application Number Title Priority Date Filing Date
ES534589A Expired ES8601303A1 (es) 1983-07-27 1984-07-24 Procedimiento para preparar un polipeptido

Country Status (15)

Country Link
US (1) US5457033A (es)
EP (1) EP0133282B1 (es)
JP (1) JPH07121229B2 (es)
KR (1) KR850001287A (es)
AT (1) ATE68827T1 (es)
AU (1) AU572171B2 (es)
CA (1) CA1224166A (es)
DE (2) DE3327007A1 (es)
DK (1) DK171940B1 (es)
ES (1) ES8601303A1 (es)
GR (1) GR82436B (es)
IE (1) IE57664B1 (es)
IL (1) IL72489A0 (es)
PT (1) PT78979A (es)
ZA (1) ZA845779B (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61291598A (ja) * 1985-06-18 1986-12-22 Sagami Chem Res Center 魚類カルシトニン誘導体及びその製造方法
JPS61257187A (ja) * 1985-05-11 1986-11-14 Sagami Chem Res Center サケカルシトニン誘導体遺伝子
DE3436819A1 (de) * 1984-10-06 1986-04-17 Hoechst Ag, 6230 Frankfurt Arzneimittel mit grf-wirkung
IT1234977B (it) * 1985-03-22 1992-06-09 Serono Ist Farm Espressione in e. coli polipeptidi ibridi contenenti la sequenza del fattore di rilascio dell'ormone della crescita
FR2587359B1 (fr) * 1985-05-28 1987-12-24 Sanofi Sa Derives non amides de la somatocrinine et procede de preparation par genie genetique
DK382886A (da) * 1985-08-12 1987-02-13 Syntex Inc Fremgangsmaade til fremstilling af hormonfaktorderivater
GB8523156D0 (en) * 1985-09-19 1985-10-23 Celltech Ltd Polypeptide production
US4865974A (en) * 1985-09-20 1989-09-12 Cetus Corporation Bacterial methionine N-terminal peptidase
DE3632037A1 (de) * 1986-09-20 1988-04-07 Hoechst Ag Gentechnisches verfahren zur herstellung von salm-calcitonin sowie mittel zur durchfuehrung dieses verfahrens
US5789234A (en) * 1987-08-14 1998-08-04 Unigene Laboratories, Inc. Expression systems for amidating enzyme
JPH01144981A (ja) * 1987-12-02 1989-06-07 Wakunaga Pharmaceut Co Ltd 蛋白質の製造
DE69108192T2 (de) * 1990-12-10 1995-07-20 Hoffmann La Roche Verfahren zur enzymatischen Herstellung von GRF(1-44)NH2.
GB0512610D0 (en) 2005-06-18 2005-07-27 Hexcel Composites Ltd Composite material

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3381567D1 (de) * 1982-11-04 1990-06-21 Hoffmann La Roche Hestellung von rekombinanten wachstumsausloesenden faktoren.
US4581168A (en) * 1983-02-21 1986-04-08 Sanofi Synthesis of hpGRF (Somatocrinin) in liquid phase and intermediate peptides
IL71150A (en) * 1983-03-07 1987-02-27 Sparamedica Ag Growth hormone releasing factor analogs,their preparation and pharmaceutical compositions containing them
ZA844380B (en) * 1983-07-05 1985-01-30 Salk Inst For Biological Studi Dna encoding a grf precursor
AU4727385A (en) * 1984-08-17 1986-03-07 Sagami Chemical Research Center Fish calcitonin derivative, process for its preparation, and gene system therefor

Also Published As

Publication number Publication date
ES534589A0 (es) 1985-10-16
US5457033A (en) 1995-10-10
DK365484A (da) 1985-01-28
KR850001287A (ko) 1985-03-18
JPH07121229B2 (ja) 1995-12-25
DK171940B1 (da) 1997-08-18
ATE68827T1 (de) 1991-11-15
AU3121784A (en) 1985-01-31
EP0133282B1 (de) 1991-10-23
EP0133282A1 (de) 1985-02-20
DE3327007A1 (de) 1985-02-07
JPS6049798A (ja) 1985-03-19
IL72489A0 (en) 1984-11-30
IE841930L (en) 1985-01-27
DK365484D0 (da) 1984-07-26
IE57664B1 (en) 1993-02-24
ZA845779B (en) 1985-02-27
CA1224166A (en) 1987-07-14
DE3485199D1 (es) 1991-11-28
AU572171B2 (en) 1988-05-05
PT78979A (de) 1984-08-01
GR82436B (es) 1984-12-13

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20050525