ES2367979T3 - Procedimiento para detectar y enumerar microorganismos rápidamente en preparaciones de células de mamíferos usando la bioluminiscencia del atp. - Google Patents
Procedimiento para detectar y enumerar microorganismos rápidamente en preparaciones de células de mamíferos usando la bioluminiscencia del atp. Download PDFInfo
- Publication number
- ES2367979T3 ES2367979T3 ES00930821T ES00930821T ES2367979T3 ES 2367979 T3 ES2367979 T3 ES 2367979T3 ES 00930821 T ES00930821 T ES 00930821T ES 00930821 T ES00930821 T ES 00930821T ES 2367979 T3 ES2367979 T3 ES 2367979T3
- Authority
- ES
- Spain
- Prior art keywords
- atp
- mammalian
- membrane
- cells
- microorganisms
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title claims abstract description 33
- 238000002360 preparation method Methods 0.000 title claims abstract description 30
- 244000005700 microbiome Species 0.000 title claims abstract description 21
- 241000124008 Mammalia Species 0.000 title claims description 3
- 210000004962 mammalian cell Anatomy 0.000 claims abstract description 28
- 210000004027 cell Anatomy 0.000 claims abstract description 27
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 25
- 230000000813 microbial effect Effects 0.000 claims abstract description 22
- 239000012528 membrane Substances 0.000 claims abstract description 20
- 239000003599 detergent Substances 0.000 claims abstract description 16
- 230000003301 hydrolyzing effect Effects 0.000 claims abstract description 9
- 238000001914 filtration Methods 0.000 claims abstract description 8
- 230000002209 hydrophobic effect Effects 0.000 claims abstract description 6
- 230000003204 osmotic effect Effects 0.000 claims abstract description 5
- 230000035939 shock Effects 0.000 claims abstract description 5
- 230000002934 lysing effect Effects 0.000 claims abstract description 4
- 238000001035 drying Methods 0.000 claims abstract description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 12
- 238000000605 extraction Methods 0.000 claims description 12
- 108091006112 ATPases Proteins 0.000 claims description 8
- 102000057290 Adenosine Triphosphatases Human genes 0.000 claims description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 8
- 108090000790 Enzymes Proteins 0.000 claims description 7
- 102000004190 Enzymes Human genes 0.000 claims description 7
- 238000001514 detection method Methods 0.000 claims description 7
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims description 6
- 239000005089 Luciferase Substances 0.000 claims description 6
- 239000000908 ammonium hydroxide Substances 0.000 claims description 6
- 108060001084 Luciferase Proteins 0.000 claims description 5
- 229920004890 Triton X-100 Polymers 0.000 claims description 5
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 5
- 229920000053 polysorbate 80 Polymers 0.000 claims description 5
- 239000013504 Triton X-100 Substances 0.000 claims description 4
- IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 claims description 3
- CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 claims description 3
- BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 claims description 3
- DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 claims description 3
- 230000035899 viability Effects 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 238000005406 washing Methods 0.000 abstract description 2
- 150000001875 compounds Chemical class 0.000 abstract 2
- 230000003100 immobilizing effect Effects 0.000 abstract 1
- ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 description 32
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 description 32
- 238000005415 bioluminescence Methods 0.000 description 8
- 230000029918 bioluminescence Effects 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 230000009089 cytolysis Effects 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical class CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical class CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 description 3
- 238000011109 contamination Methods 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 241000254158 Lampyridae Species 0.000 description 2
- 239000012901 Milli-Q water Substances 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
- 230000008020 evaporation Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- OTMSDBZUPAUEDD-UHFFFAOYSA-N Ethane Chemical class CC OTMSDBZUPAUEDD-UHFFFAOYSA-N 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical class C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 239000005977 Ethylene Chemical class 0.000 description 1
- 241000204031 Mycoplasma Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 150000007514 bases Chemical class 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 238000009534 blood test Methods 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 229940030156 cell vaccine Drugs 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 230000001332 colony forming effect Effects 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 210000001822 immobilized cell Anatomy 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 238000006241 metabolic reaction Methods 0.000 description 1
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical class C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 1
- 125000000325 methylidene group Chemical class [H]C([H])=* 0.000 description 1
- 239000012982 microporous membrane Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229920002113 octoxynol Polymers 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 238000013190 sterility testing Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/22—Testing for sterility conditions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2304/00—Chemical means of detecting microorganisms
- C12Q2304/60—Chemiluminescent detection using ATP-luciferin-luciferase system
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Toxicology (AREA)
- Epidemiology (AREA)
- Public Health (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13591099P | 1999-05-25 | 1999-05-25 | |
| US135910P | 1999-05-25 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2367979T3 true ES2367979T3 (es) | 2011-11-11 |
Family
ID=22470327
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES00930821T Expired - Lifetime ES2367979T3 (es) | 1999-05-25 | 2000-05-19 | Procedimiento para detectar y enumerar microorganismos rápidamente en preparaciones de células de mamíferos usando la bioluminiscencia del atp. |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US6465201B1 (fr) |
| EP (1) | EP1185616B1 (fr) |
| JP (1) | JP3811616B2 (fr) |
| AT (1) | ATE515561T1 (fr) |
| AU (1) | AU4857700A (fr) |
| ES (1) | ES2367979T3 (fr) |
| WO (1) | WO2000071675A1 (fr) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7588886B2 (en) * | 2001-01-26 | 2009-09-15 | Millipore Corporation | Process for the enumeration and identification of microorganisms |
| EP1511836A1 (fr) * | 2002-06-07 | 2005-03-09 | Millipore Corporation | Trousse et procede d'examen microbiologique sur site d'un echantillon de liquide |
| JP4987469B2 (ja) * | 2003-04-17 | 2012-07-25 | キャンブレックス・バイオ・サイエンス・ノッティンガム・リミテッド | 酢酸キナーゼまたはカルバメートキナーゼ活性を測定することによる、マイコプラズマを検出するためのアッセイ |
| US20050250138A1 (en) * | 2004-05-04 | 2005-11-10 | Millipore Corporation | Process for the enumeration and identification of microorganisms |
| US7806856B2 (en) * | 2005-04-22 | 2010-10-05 | Accessclosure, Inc. | Apparatus and method for temporary hemostasis |
| US20100129840A1 (en) * | 2006-10-13 | 2010-05-27 | Charm Scineces, Inc | Method and Apparatus for Reducing Luminescent Test Result Interferences |
| CN102325596B (zh) | 2008-12-31 | 2015-04-29 | 3M创新有限公司 | 使用微粒进行的活生物负载检测 |
| JP2012513768A (ja) | 2008-12-31 | 2012-06-21 | スリーエム イノベイティブ プロパティズ カンパニー | サンプリング装置及び微生物を濃縮するための方法 |
| EP2333105A1 (fr) * | 2009-12-08 | 2011-06-15 | Koninklijke Philips Electronics N.V. | Lyse sélective de cellules |
| CN102770208B (zh) | 2009-12-30 | 2016-02-03 | 3M创新有限公司 | 使用微粒进行的活生物负载检测 |
| DE102011077238B4 (de) * | 2011-06-09 | 2016-03-31 | Bayerische Motoren Werke Aktiengesellschaft | Verfahren zum Nachweis von Mikroorganismen in einem Hohlraumkonservierungsmittel |
| JP5960421B2 (ja) * | 2011-12-05 | 2016-08-02 | 株式会社日立ハイテクノロジーズ | 細胞の測定方法及び細胞の測定用試薬 |
| CA2949732C (fr) | 2014-06-13 | 2023-01-17 | Q-Linea Ab | Procede pour la detection et la caracterisation d'un micro-organisme |
| GB201507026D0 (en) | 2015-04-24 | 2015-06-10 | Linea Ab Q | Medical sample transportation container |
| GB2554767A (en) | 2016-04-21 | 2018-04-11 | Q Linea Ab | Detecting and characterising a microorganism |
| GB201703383D0 (en) | 2017-03-02 | 2017-04-19 | Gargle Tech Ltd | Testing for particulates |
| JP7062552B2 (ja) | 2018-08-06 | 2022-05-06 | 株式会社日立製作所 | 微生物検査 |
| EP3846939B1 (fr) | 2018-09-05 | 2023-05-03 | Hero Scientific Ltd | Détection de particules |
| CN109187395A (zh) * | 2018-10-26 | 2019-01-11 | 河南海利未来科技有限公司 | 一种洗涤剂中总磷含量的测定方法 |
| US12449336B2 (en) | 2020-03-11 | 2025-10-21 | Hero Scientific Ltd. | Testing devices |
| WO2022149135A2 (fr) | 2021-01-06 | 2022-07-14 | Hero Scientific Ltd. | Dispositifs d'échantillonnage à filtration |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0025351A1 (fr) | 1979-09-05 | 1981-03-18 | Dynatech Ag | Détection de bactéries dans l'urine |
| CH678065A5 (en) | 1988-04-13 | 1991-07-31 | Hamilton Bonaduz Ag | Quantitative determn. of adenosine-tri:phosphate - by a bio-luminescent reaction capable of determining ATP in somatic and/or microbial cells |
| DE563858T1 (de) * | 1992-04-01 | 1994-03-03 | Nihon Millipore Kogyo K K | Verfahren zur Bestimmung der Anzahl lebender Mikroorganismen. |
| JP3228812B2 (ja) * | 1993-02-10 | 2001-11-12 | 日本マイクロリス株式会社 | 生菌数を測定する方法 |
| JP3547882B2 (ja) * | 1995-12-28 | 2004-07-28 | キッコーマン株式会社 | Ａｔｐ消去剤、ａｔｐ消去法、それを用いた生物細胞測定試薬及び生物細胞測定法 |
| US5908751A (en) * | 1996-04-26 | 1999-06-01 | Toyo Ink Mfg. Co., Ltd. | Method for detecting and/or determining ATP from microorganism cells in a sample |
| AU5303499A (en) * | 1998-10-28 | 2000-05-15 | Nikon Corporation | Method and device for producing mask |
-
2000
- 2000-05-19 JP JP2000620054A patent/JP3811616B2/ja not_active Expired - Lifetime
- 2000-05-19 WO PCT/US2000/013758 patent/WO2000071675A1/fr not_active Ceased
- 2000-05-19 AU AU48577/00A patent/AU4857700A/en not_active Abandoned
- 2000-05-19 EP EP00930821A patent/EP1185616B1/fr not_active Expired - Lifetime
- 2000-05-19 AT AT00930821T patent/ATE515561T1/de not_active IP Right Cessation
- 2000-05-19 ES ES00930821T patent/ES2367979T3/es not_active Expired - Lifetime
- 2000-05-19 US US09/574,124 patent/US6465201B1/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JP3811616B2 (ja) | 2006-08-23 |
| EP1185616B1 (fr) | 2011-07-06 |
| EP1185616A1 (fr) | 2002-03-13 |
| ATE515561T1 (de) | 2011-07-15 |
| US6465201B1 (en) | 2002-10-15 |
| EP1185616A4 (fr) | 2003-04-23 |
| WO2000071675A1 (fr) | 2000-11-30 |
| JP2003500045A (ja) | 2003-01-07 |
| AU4857700A (en) | 2000-12-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2367979T3 (es) | Procedimiento para detectar y enumerar microorganismos rápidamente en preparaciones de células de mamíferos usando la bioluminiscencia del atp. | |
| Volk | Screening of microalgal culture media for the presence of algicidal compounds and isolation and identification of two bioactive metabolites, excreted by the cyanobacteria Nostoc insulare and Nodularia harveyana | |
| Johnson et al. | Genetic variation of alcohol dehydrogenase in Drosophila melanogaster | |
| JP5237305B2 (ja) | 透過性を有するメンブレン上の微生物の検知及び識別 | |
| Bergman et al. | Phycomyces | |
| DE69020555T2 (de) | Fällungstest für mikroorganismen. | |
| CA2447750A1 (fr) | Methode amelioree pour la detection et l'identification de micro- organismes responsables de maladies infectieuses | |
| CN103013822A (zh) | 一种水产弧菌快速药敏检测试剂盒 | |
| CA2507079A1 (fr) | Procede de detection universelle de microorganismes et milieu reactionnel permettant la mise en oeuvre du procede | |
| ES2152913T3 (es) | Medio para el analisis cualitativo y cuantitativo de las poblaciones microbianas potencialmente presentes en una muestra. | |
| ES2573405T3 (es) | Detección de bacterias y hongos | |
| CN101921873B (zh) | 对虾传染性肌肉坏死病毒现场快速高灵敏检测试剂盒及检测方法 | |
| CN1918305B (zh) | 核酸检测方法及其应用 | |
| EP3269822B1 (fr) | Procédés pour détecter une activité biologique | |
| JP2013048611A (ja) | ウエルシュ菌の検出方法およびウエルシュ菌検出用のキット | |
| JP2010213724A (ja) | 微生物細胞壁の透過性を増大させるための組成物および膜上で前記微生物を検出するための方法 | |
| CN109943621B (zh) | 来自弧菌属的碱性几丁质酶在真菌染色中的应用 | |
| CN107383927A (zh) | 一种叠氮吲哚二甲川菁染料分子及应用 | |
| CN111307775A (zh) | 一种应用刃天青试剂检测体外3d培养条件下pbmc活性的方法 | |
| JPH1156393A (ja) | Ａｔｐの測定法およびａｔｐ測定用試薬キット | |
| JP2008125457A (ja) | 微生物の検出方法 | |
| EP4198141A1 (fr) | Procédé de détection et de quantification éventuelle de micro-organismes | |
| SU722242A1 (ru) | Способ дифференциации холерных вибрио-HOB OT НЕгАзООбРАзующиХ АэРОМОНАд | |
| RU2061045C1 (ru) | Способ количественного определения микробной обсемененности биологического жидкого образца | |
| Srivastava | Elementary Microbiology |