ES2129365B1 - Metodo para la deteccion de secuencias especificas de acidos nucleicos en presencia de un vector util como control positivo interno. - Google Patents
Metodo para la deteccion de secuencias especificas de acidos nucleicos en presencia de un vector util como control positivo interno.Info
- Publication number
- ES2129365B1 ES2129365B1 ES009701600A ES9701600A ES2129365B1 ES 2129365 B1 ES2129365 B1 ES 2129365B1 ES 009701600 A ES009701600 A ES 009701600A ES 9701600 A ES9701600 A ES 9701600A ES 2129365 B1 ES2129365 B1 ES 2129365B1
- Authority
- ES
- Spain
- Prior art keywords
- nucleic acid
- detection
- acid sequences
- positive control
- specific nucleic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title abstract 4
- 238000001514 detection method Methods 0.000 title abstract 3
- 150000007523 nucleic acids Chemical group 0.000 title abstract 3
- 239000013641 positive control Substances 0.000 title abstract 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract 1
- 102000040650 (ribonucleotides)n+m Human genes 0.000 abstract 1
- 241000450599 DNA viruses Species 0.000 abstract 1
- 206010028980 Neoplasm Diseases 0.000 abstract 1
- 201000011510 cancer Diseases 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 238000003752 polymerase chain reaction Methods 0.000 abstract 1
- 230000003612 virological effect Effects 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Método para la detección de secuencias especificas de ácidos nucleidos en presencia de un vector útil como control positivo interno. El método comprende amplificar una secuencia específica presente en una muestra por medio de la reacción en cadena de la polimerasa en presencia de un vector control positivo interno que comprende la secuencia nucleótidica de al menos uno de los iniciadores utilizados en la PCR. El método es útil para la detección de secuencias específicas de ácido nucleico derivadas de microorganismos, células normales o cancerosas, virus RNA o DNA de cadena sencilla o doble o transcritos virales.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES009701600A ES2129365B1 (es) | 1997-07-18 | 1997-07-18 | Metodo para la deteccion de secuencias especificas de acidos nucleicos en presencia de un vector util como control positivo interno. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES009701600A ES2129365B1 (es) | 1997-07-18 | 1997-07-18 | Metodo para la deteccion de secuencias especificas de acidos nucleicos en presencia de un vector util como control positivo interno. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2129365A1 ES2129365A1 (es) | 1999-06-01 |
| ES2129365B1 true ES2129365B1 (es) | 2000-04-01 |
Family
ID=8300113
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES009701600A Expired - Fee Related ES2129365B1 (es) | 1997-07-18 | 1997-07-18 | Metodo para la deteccion de secuencias especificas de acidos nucleicos en presencia de un vector util como control positivo interno. |
Country Status (1)
| Country | Link |
|---|---|
| ES (1) | ES2129365B1 (es) |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5219727A (en) * | 1989-08-21 | 1993-06-15 | Hoffmann-Laroche Inc. | Quantitation of nucleic acids using the polymerase chain reaction |
| GB9116042D0 (en) * | 1991-07-24 | 1991-09-11 | Royal Free Hosp School Med | Diagnostic method |
| PT528306E (pt) * | 1991-08-15 | 2000-05-31 | Hoffmann La Roche | Iniciadores e sondas de micobacterias |
| EP0586112A3 (en) * | 1992-08-14 | 1994-09-14 | Pharma Gen S A | Control of pcr mediated detection of micro-organisms |
-
1997
- 1997-07-18 ES ES009701600A patent/ES2129365B1/es not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| ES2129365A1 (es) | 1999-06-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EC2A | Search report published |
Date of ref document: 19990601 Kind code of ref document: A1 Effective date: 19990601 |
|
| PC2A | Transfer of patent | ||
| FD2A | Announcement of lapse in spain |
Effective date: 20171026 |