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EP4643110A1 - Agencement de déplacement de coupe, microtome et procédé associé - Google Patents

Agencement de déplacement de coupe, microtome et procédé associé

Info

Publication number
EP4643110A1
EP4643110A1 EP22844233.1A EP22844233A EP4643110A1 EP 4643110 A1 EP4643110 A1 EP 4643110A1 EP 22844233 A EP22844233 A EP 22844233A EP 4643110 A1 EP4643110 A1 EP 4643110A1
Authority
EP
European Patent Office
Prior art keywords
movement
adjustment device
sample
holder
actuator
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP22844233.1A
Other languages
German (de)
English (en)
Inventor
Michael Zimmermann
Matthias Katzengruber
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Leica Mikrosysteme GmbH
Original Assignee
Leica Mikrosysteme GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Leica Mikrosysteme GmbH filed Critical Leica Mikrosysteme GmbH
Publication of EP4643110A1 publication Critical patent/EP4643110A1/fr
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
    • G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome

Definitions

  • the present invention relates to a cutting movement arrangement for a microtome, such a microtome and a method for producing sample sections by means of a microtome.
  • sample sections can be cut from a sample block using a microtome and then placed on a sample carrier. In this case, the preparation of sample sections can sometimes be difficult or impractical due to certain predetermined processes in the microtome.
  • the sheath movement arrangement comprises one of a knife holder and a sample holder.
  • the other of these two is typically part of the microtome with which the sheath movement arrangement can be used.
  • the sheathing movement arrangement has an adjustment mechanism by means of which one of the knife holder and the sample holder can be moved in a return movement.
  • the sheathing movement arrangement has the knife holder.
  • the sheath movement arrangement has an additional adjustment device that is different from the adjustment mechanism, i.e. the additional adjustment device in particular has no components in common with the adjustment mechanism.
  • the sheath movement arrangement is also designed in such a way that the adjustment mechanism can be caused to at least partially carry out the return movement by means of the additional adjustment device.
  • the additional adjustment device provides an additional possibility of at least partially enabling the return movement, in particular independently of the adjustment mechanism.
  • the reset movement can therefore be carried out at any time or at least more frequently than with the adjustment mechanism itself.
  • the additional adjustment device is designed such that the additional adjustment device can be brought into a first position and into a second position that is different from the first position.
  • the cutting movement arrangement is designed such that when the additional adjustment device is brought into the first position, the adjustment mechanism is not influenced by the additional adjustment device.
  • the cutting movement arrangement is designed to cause the adjustment mechanism to at least partially carry out the return movement.
  • the sheath movement arrangement is designed such that when the additional adjustment device is brought into the second position, the additional adjustment device can be brought into mechanical contact with the adjustment mechanism in order to apply force to the adjustment mechanism and cause it to at least partially carry out the return movement.
  • the additional adjustment device has an actuator and is designed such that the additional adjustment device can be brought into the first position and the second position by actuating the actuator.
  • An actuator allows a safe and targeted actuation of the additional adjustment device so that it can act on the adjustment mechanism.
  • the actuator has an electric motor or is designed as an electric motor.
  • the additional adjustment device can be actuated flexibly, for example from a distance or using a button or the like.
  • the additional adjustment device further comprises a base body and a lever mounted in the base body.
  • the additional adjustment device comprises a contact area with which the additional adjustment device can be brought into mechanical contact with the adjustment mechanism.
  • the contact area is formed on the lever or is operatively connected to the lever, and the lever can be actuated by means of the actuator.
  • the additional adjustment device further comprises a connecting rod mechanism.
  • the actuator and the lever are operatively connected to one another via the connecting rod mechanism, so that the lever can be actuated by means of the actuator.
  • a connecting rod mechanism allows a simple transmission of force from the actuator to the adjustment mechanism.
  • This makes it easy to convert a rotary motion of an actuator (as is the case with an electric motor, for example) into a linear or other suitable motion for the lever.
  • the cutting movement arrangement has an interface for an actuator that is different from the additional adjustment device to the adjustment device and is designed in such a way that when the adjustment device is actuated via the interface, one of the knife holder and the sample holder can only be moved within the scope of a predetermined movement sequence.
  • the actuator can have a handwheel and/or a motor, for example.
  • the predetermined movement sequence includes an advancing movement, a cutting movement, the return movement and a counter-cutting movement. In particular, the movements mentioned must then be carried out in this order; a deviation from this is not provided for in the adjustment mechanism or is not possible using the adjustment mechanism itself.
  • the additional adjustment device also enables a faster creation of several sample sections with regular sample blocks, since the reset movement can be carried out at any time - or at least more frequently - which means that a complete run-through of the movement sequence is no longer necessary.
  • the cutting movement arrangement is designed in such a way that the additional adjustment device can be used to cause the adjustment mechanism to at least partially carry out the reset movement if one of the knife holder and sample holder is not in the reset movement within the scope of the predetermined movement sequence. If a reset movement is already taking place within the scope of the predetermined movement sequence, a further initiation of the reset movement is not or no longer necessary.
  • the adjustment mechanism has a reset lever.
  • the sheath movement arrangement is designed such that when the reset lever is actuated, the reset movement is at least partially carried out.
  • the sheath movement arrangement is designed such that the additional adjustment device can be brought into contact with the reset lever in order to cause the adjustment mechanism to at least partially carry out the reset movement.
  • the adjustment mechanism further comprises a holder on which one of the knife holder and the sample holder is arranged directly or indirectly.
  • the adjustment mechanism is designed such that the holder is moved when the reset movement is carried out.
  • the cutting movement arrangement is designed such that the additional adjustment device can be brought into contact with the holder directly or indirectly in order to cause the adjustment mechanism to at least partially carry out the reset movement.
  • the action of the additional adjustment device on the holder allows a particularly simple implementation in order to carry out the reset movement.
  • the sheath movement arrangement is designed such that the additional adjustment device can be brought into contact with the holder indirectly via the reset lever in order to cause the adjustment mechanism to at least partially carry out the reset movement.
  • This type of adjustment mechanism with a holder for a knife holder or sample holder and with a reset lever allows the additional adjustment device to act on the holder via the reset lever, making it particularly easy to carry out the reset movement.
  • a further embodiment of the invention relates to a microtome which has a cutting movement arrangement according to one of the embodiments explained.
  • the microtome also has the other of a knife holder and a sample holder, wherein the knife holder and the sample holder are movable relative to one another in the return movement by means of the adjustment mechanism.
  • the microtome further comprises an actuator that is different from the additional adjustment device, comprising, for example, a handwheel and/or a motor.
  • the actuator is operatively connected to the adjustment mechanism via the interface in such a way that when the actuator is actuated, the knife holder and the sample holder can only be moved relative to one another within the scope of the predetermined movement sequence.
  • a further embodiment of the invention relates to the use of an additional adjustment device with a microtome, wherein the microtome has a knife holder, a sample holder and an adjustment mechanism, wherein the knife holder and the sample holder can be moved relative to one another in a return movement by means of the adjustment mechanism.
  • the additional adjustment device is designed in such a way that the adjustment mechanism can be caused to at least partially carry out the return movement.
  • the additional adjustment device and/or the microtome can in particular be designed according to one of the embodiments explained.
  • a further embodiment of the invention relates to a method for producing sample sections using a microtome, wherein the microtome has a knife holder, a sample holder, an adjustment mechanism and an actuator.
  • the knife holder and the sample holder are in this case arranged in an advancing movement, a cutting movement, a resetting movement and a counter-cutting movement.
  • the actuator is operatively connected to the adjustment mechanism in such a way that when the actuator is actuated, the knife holder and the sample holder can only be moved relative to one another within the framework of a predetermined movement sequence, wherein the predetermined movement sequence comprises the advancing movement, the cutting movement, the resetting movement and the counter-cutting movement.
  • a knife is or will be arranged in the knife holder, and a sample block is or will be arranged in the sample holder. If required, the resetting movement is at least partially carried out by means of an additional adjustment device that is different from the adjustment mechanism.
  • the additional adjustment device and/or the microtome can in particular be designed according to one of the exemplary embodiments explained.
  • the method comprises the following steps: a first execution of the cutting movement by actuating the actuator so that a first sample section is cut off from the sample block by means of the knife.
  • a resetting movement is carried out by means of the additional adjustment device so that the sample block is removed from the knife.
  • a counter-cutting movement is carried out by actuating the actuator.
  • a second execution of the cutting movement by actuating the actuator so that a second sample section is cut off from the sample block by means of the knife.
  • the additional adjustment device is therefore used to intervene in the predetermined movement sequence, which is only made possible by the additional adjustment device in order to produce sample sections more quickly and flexibly.
  • the reset movement is carried out by means of the additional adjustment device before the first execution of the cutting movement according to the predetermined movement sequence is completed.
  • the actually fixed predetermined movement sequence is therefore deliberately interrupted, whereby the sample sections can be produced more quickly.
  • the sample block has two block parts, the sample block being arranged in the sample holder in such a way that the two block parts are located one behind the other in relation to the cutting movement. The first sample cut is then cut from only one of the two block parts of the sample block, with nothing being cut from the other of the two parts of the sample block when the cutting movement is carried out for the first time. Only the additional adjustment device allows several sample cuts to be made from only one of the two parts without cutting the other block part.
  • At least one of the first and second cutting movements is carried out by actuating the actuator in a first direction, with the counter-cutting movement being carried out by actuating the actuator in a second direction of the actuator, opposite to the first direction.
  • the actually predetermined direction of rotation is therefore deliberately reversed, whereby the sample sections can be produced more quickly. This is also only possible thanks to the additional adjustment device.
  • Figure 1a shows schematically a sheath movement arrangement according to an embodiment of the invention in a perspective view.
  • Figure 1b shows schematically a part of the sheath movement arrangement from Figure 1a in a sectional view.
  • Figure 2 shows schematically a sheath movement arrangement according to an embodiment of the invention in two different positions.
  • Figure 3a shows schematically a sheath movement arrangement according to an embodiment of the invention in a perspective view.
  • Figure 3b shows schematically a part of the sheath movement arrangement from Figure 3a in a sectional view.
  • Figure 4 shows schematically a microtome according to an embodiment of the invention.
  • FIG. 5 shows schematically a sample block to explain an embodiment of the invention.
  • Figure 6 shows schematically a sequence of a method according to an embodiment of the invention.
  • Figure 1a shows a schematic perspective view of a sheathing movement arrangement 100 according to an embodiment of the invention.
  • Figure 1b shows a section view of part of the sheathing movement arrangement 100 from Figure 1a.
  • Figures 1a and 1b will be described in general below.
  • the sheathing movement arrangement 100 can be used in or with a microtome.
  • Figure 4 For an explanation of how the sheathing movement arrangement 100 or another sheathing movement arrangement can be used in a microtome, see Figure 4 and the associated description.
  • the shearing movement arrangement 100 has a sample holder 102.
  • a sample block can be inserted, from which Sample sections are to be cut off and fixed.
  • a knife by means of which the sample sections can then be cut, can be arranged in a knife holder, which in turn can be arranged elsewhere in the microtome.
  • the knife holder and sample holder can also be interchanged, ie the shearing movement arrangement could have a knife holder instead of the sample holder.
  • the cutting movement arrangement 100 has an adjustment mechanism 110, by means of which the sample holder 102 can be moved in a return movement R. By means of this return movement R, a sample block, when arranged in the sample holder 102, can be moved away from a knife (not shown here).
  • the adjustment mechanism 110 has a holder 114 on which the sample holder 102 is arranged directly or indirectly. The adjustment mechanism 110 is then designed such that the holder 114 is moved when the return movement R is carried out. Because the sample holder 102 is arranged (or fastened) directly or indirectly on the holder 114, a movement of the holder 114 leads to a movement of the sample holder 102.
  • the adjustment mechanism 110 has a reset lever 112, wherein the sheath movement arrangement 100 is designed such that when the reset lever 112 is actuated, the reset movement R is at least partially carried out. In the embodiment shown, actuation of the reset lever 112 leads to a movement of the holder.
  • the adjustment mechanism 110 has an interface 116 for an actuator 130 to the adjustment mechanism 110.
  • the actuator 130 has a handwheel 132 and a motor or electric motor 134, which can act on a shaft or axis 136 of the actuator. Turning the handwheel 132 causes the shaft 136 to rotate, and actuating the electric motor 134 also causes the shaft 136 to rotate.
  • the electric motor is connected to the shaft 136 via a belt 138, for example.
  • the actuator 130 could also have, for example, only the handwheel 132 or only the electric motor 134.
  • a handwheel could also be provided which is not mechanically connected to the shaft 136, but is electrically connected to the electric motor 134, for example via an encoder, so that a rotation of the handwheel leads to a control of the electric motor.
  • the interface 116 is designed as a mechanical interface for the shaft 136 of the actuator 130, in particular as or with a bushing for the shaft 136.
  • the adjustment mechanism 110 has two cams 118.1 and 118.2.
  • the cams 118.1 and 118.2 can be connected to one another and have the interface 116.
  • the cam 118.1 is operatively connected to the reset lever 112 and the cam 118.2 is operatively connected to a support component 113 of the adjustment mechanism 110.
  • the functioning of the adjustment mechanism 110 will be explained below using the embodiment of the cutting movement arrangement 100 shown in Figures 1a and 1b.
  • the actuation of the actuator 130 (either by the handwheel 132 or the electric motor 134) leads to a rotation of the shaft 136.
  • a rotation can take place in a first direction of rotation B1 or in a second, opposite direction of rotation B2.
  • a predetermined movement sequence is carried out which includes an advancing movement V, a cutting movement S, the return movement R and a counter-cutting movement G, which are indicated in Figure 1b, in particular in this order.
  • the advance movement V results in the sample block (not shown) in the sample holder 102 being moved towards the knife (not shown). This can be done, for example, by the reset lever 112 not being raised by the cam 118.1, so that a preload force presses the holder 114 or the sample holder 102 in the direction of the advance movement V (also called feed movement).
  • the cam 118.2 results in the support component 113 being lowered (possibly under the action of a suitable preload force), so that the sample block (not shown) in the sample holder 102 is moved downwards towards the knife (not shown), i.e. carries out the cutting movement S in order to produce a sample cut.
  • the cam 118.1 causes the reset lever 112 to be raised at a first end 112.1 by the cam 118.1, so that the reset lever 112 rotates about a bearing axis or a bearing 112.3, so that a second end 112.2 of the reset lever 112 acts on holder 114, so that it is moved in the direction of the reset movement R.
  • the sample block (not shown) in the sample holder 102 thus performs the reset movement R.
  • the cam 118.2 causes the support component 113 to be raised so that the sample block (not shown) in the sample holder 102 is moved upwards, i.e. performs the counter-cutting movement G.
  • the movement sequence described is repeated, whereby if necessary, as part of the advancing movement V, the sample block (not shown) in the sample holder 102 is moved slightly further forwards if a sample cut is to be produced at the same point. It can also be provided that the sample block and knife are slightly displaced in the lateral direction in order to produce a sample cut at a different point.
  • the cutting movement arrangement 100 further comprises an additional adjustment device 120, which is different from the adjustment mechanism 110 and which is also different from the actuator 130 and does not act on the interface 116.
  • the cutting movement arrangement 100 is designed such that the adjustment mechanism 110 can be caused to at least partially carry out the return movement R by means of the additional adjustment device 120.
  • the return movement R is in particular the return movement described above for the sample holder 102 or a sample block inserted therein.
  • the additional adjustment device 120 is designed such that it can be brought into a first position and into a second position that is different from the first position.
  • the cutting movement arrangement 100 is designed such that when the additional adjustment device 120 is brought into the first position, the Adjustment mechanism 110 is not influenced by the additional adjustment device 120. This situation is shown in Figures 1a and 1b.
  • the sheath movement arrangement is designed to cause the adjustment mechanism 110 to at least partially carry out the return movement R when the additional adjustment device 120 is brought into the second position.
  • the additional adjustment device 120 has a lever 122, which is mounted on a base body (not shown here), for example.
  • the additional adjustment device 120 can be brought into mechanical contact with the adjustment mechanism 110 via a contact area on the lever 122, in particular with the reset lever 112, preferably at the second end 112.2 of the reset lever 112.
  • the additional adjustment device 120 can thus also be brought into contact indirectly with the holder 114.
  • the reset lever 112 can be actuated on the one hand within the framework of the predetermined movement sequence via the cam 118.1 or the actuator 130 and the interface 116, but on the other hand also independently of this via the additional adjustment device 120. Regardless of the current point in the movement sequence, the reset lever 112 can therefore be actuated by means of the additional adjustment device 120 and thus the reset movement R can be initiated.
  • the reset lever 112 is also rotated about the bearing point 112.3 by means of the additional adjustment device 120.
  • the actuation of the additional adjustment device 120 may be ineffective - this is not detrimental to the functionality, however, since the return movement R can still be carried out in a targeted manner in all other situations if required.
  • FIG 2 shows a schematic representation of a sheath movement arrangement 200 according to a further embodiment of the invention in two different positions (top and bottom).
  • the sheath movement arrangement 200 can also be, for example, the sheath movement arrangement 100 according to Figures 1a and 1b, but unlike there, the sheath movement arrangement 200 is shown here in a somewhat simplified manner in order to explain the basic functional principle of the additional adjustment device 220.
  • a sample holder 202 and an adjustment mechanism 210 with a holder 114 and a reset lever 112 are shown here, which can basically interact in the same way as explained for the sheath movement arrangement 100.
  • a sample block 204 is shown here, which is arranged or fastened in the sample holder 202.
  • a knife 240 is shown, which does not have to be part of the sheath movement arrangement 200, but can be arranged in a suitable manner, for example in the microtome (not shown here).
  • the additional adjustment device 220 is brought into a first position P1.
  • the adjustment mechanism 210 is not influenced by the additional adjustment device 220.
  • the additional adjustment device 220 and in particular the lever 212 can be out of contact with the adjustment mechanism 210 or its reset lever 212.
  • the lever 212 can also touch the reset lever 212 (be in contact with it), but without exerting a force on it, or at least not a force that would be sufficient to actuate the reset lever 212.
  • the additional adjustment device 220 is shown in a second position P2.
  • the adjustment mechanism 210 of the additional adjustment device 220 is caused to carry out the return movement R.
  • the cutting movement arrangement 200 is designed such that when the additional adjustment device 220 is brought into the second position P2, the additional adjustment device 220 can be brought into mechanical contact with the adjustment mechanism 210 - and is brought into contact when executed - in order to apply force to the adjustment mechanism 210 and to cause it to at least partially carry out the return movement R. This is shown as an example in Figure 2.
  • FIG. 3a shows a schematic perspective view of a sheath movement arrangement 300 according to a further embodiment of the invention. A section of it is also shown enlarged.
  • Figure 3b shows a section of the sheath movement arrangement 300 from Figure 3a. Figures 3a and 3b will be described in detail below.
  • the sheath movement arrangement 300 can also be, for example, the sheath movement arrangement 100 according to Figures 1a and 1b or the sheath movement arrangement 200 according to Figure 2, but unlike there, a larger part of the additional adjustment device 320 is shown in more detail in the sheath movement arrangement 300 in order to explain its functional principle in more detail.
  • a reset lever 312 with a first end 312.1, a second end 312.2 and a bearing 312.3, a holder 314, an interface 316 and a cam 318.1 are shown here as examples of an adjustment mechanism 310 of the sheath movement arrangement 300. As already explained with reference to Figures 1a, 1b and 2, a predetermined movement sequence can thus be carried out.
  • the additional adjustment device 320 has a base body 328 and a lever 322 mounted in the base body 328.
  • the additional adjustment device 320 also has an actuator 316, for example designed as an electric motor, and a connecting rod mechanism 324.
  • the actuator 326 and the lever 322 are operatively connected to one another via the connecting rod mechanism 324, so that the lever 122 can be actuated by means of the actuator 326.
  • the actuator 316 can be arranged or fastened directly or indirectly to the base body 324.
  • the connecting rod mechanism 324 can have an eccentric component 324.1, with which the connecting rod mechanism 324 is arranged on the actuator 326 or a shaft thereof.
  • the connecting rod mechanism 324 is then connected to a first end 322.1 of the lever 322 via a connecting component 324.2.
  • the lever 322 is in turn mounted on a bearing or via a bearing axis 322.3 (e.g. using a bearing shaft) in the base body 328.
  • a rotary movement of the actuator 326 can be converted into an actuation of the lever 322, so that the latter can be moved back and forth between a first position and a second position.
  • the lever 322 (and thus the additional adjustment device 320) has a contact area 322.2 with which the lever 322 (and thus the additional adjustment device 320) can be brought into contact with the reset lever 322 or the second end 322.2 there (and thus with the adjustment mechanism 310).
  • the actuation of the lever 322 generated by the actuator 326 can thus cause the adjustment mechanism 310 to at least partially carry out the reset movement.
  • the lever 322 presses with the contact area 322.2 on the second end 312.2 of the reset lever 312, ie comes into mechanical contact, which in turn presses, for example, on a pin or pin 315 of the holder 314, so that this is actuated.
  • a button (not shown here) or another actuating element can be provided, for example, which, when actuated, rotates the actuator 316 by a predetermined value in order to move the additional adjustment device 320 (by actuating the actuator) from the first to the second position.
  • actuating element when the actuating element is actuated again, the actuator 316 is rotated by a further predetermined value in order to move the additional adjustment device 320 from the second to the first position again.
  • FIG. 4 schematically shows a microtome 401 according to an embodiment of the invention.
  • the microtome 401 has a cutting movement arrangement 400 with a sample holder 402.
  • a sample block 404 is inserted into the sample holder 404.
  • the microtome 401 also has a knife holder 442 with a knife 440 inserted therein, as well as, for example, a handwheel 432 as an actuator.
  • the cutting movement arrangement 400 can be actuated by means of the handwheel 432, so that the sample block 404 or the sample holder 402 and the knife 440 or the knife holder 442 can be moved relative to one another.
  • the cutting movement arrangement 400 can be, for example, a cutting movement arrangement shown in Figures 1a, 1b, 2 and 3, so that for its description reference is made to the above.
  • the microtome 401 has, for example, a button 450 by means of which, for example, an additional adjustment device 420 of the cutting movement arrangement 400 can be actuated.
  • Figure 5 shows a schematic representation of a sample block 504 to explain an embodiment of the invention. Sample sections can be cut from the sample block 504 using a microtome, as explained with reference to Figure 4. For this purpose, the sample block 504 can be placed in a sample holder, as also already explained.
  • the sample block 504 shown here has a first block part 504.1 and a second block part 504.2.
  • the sample block can be arranged in the sample holder of a microtome in such a way that the two block parts 504.1 and 504.2 are located one behind the other with respect to the cutting movement S, as indicated in Figure 5.
  • Such a sample block with two block parts is used, for example, to be able to introduce different samples into one sample block or, in particular, to not introduce a sample into one of the two block parts, typically the second block part 504.2. So-called empty sections are then produced from the second block part 504.2.
  • a sample section can be created from the first block part 504.1, then the movement sequence can be stopped and the return movement R can be carried out using the additional adjustment device. Then a counter-cutting movement can be carried out to move the sample block back again and after deactivating the additional adjustment device, ie connecting it to the first position, a sample section can be created again from the first Block part 504.1 can be generated. The counter-cutting movement can then be carried out, for example, by turning the handwheel in the opposite direction.
  • FIG. 6 shows a schematic sequence of a method according to an embodiment of the invention.
  • a microtome with a cutting movement arrangement as explained above using various embodiments can be used.
  • the knife holder and the sample holder can be moved relative to one another by means of the adjustment mechanism in an advancing movement, a cutting movement, a resetting movement and a counter-cutting movement.
  • the actuator is operatively connected to the adjustment mechanism in such a way that when the actuator is actuated, the knife holder and the sample holder can only be moved relative to one another within the framework of a predetermined movement sequence, wherein the predetermined movement sequence includes the advancing movement, the cutting movement, the resetting movement and the counter-cutting movement.
  • a knife is arranged in the knife holder or will be arranged there, and a sample block is arranged in the sample holder or will be arranged there. If necessary, the resetting movement is then at least partially carried out by means of an additional adjustment device that is different from the adjustment mechanism.
  • a step 600 the cutting movement is first carried out by actuating the actuator, so that a first sample section is cut from the sample block by means of the knife.
  • the actuator can be rotated, for example, in a first direction or direction of rotation, as designated, for example, by B1 in Figure 1a.
  • a step 602 the reset movement is carried out using the additional adjustment device so that the sample block is removed from the knife.
  • This reset movement is carried out using the additional adjustment device in particular before the first execution of the cutting movement according to the predetermined movement sequence has ended. In other words, the cutting movement is not completed, as was also explained with reference to Figure 5.
  • the first sample cut is then cut from only one of the two block parts of the sample block, with nothing being cut from the other of the two parts of the sample block when the cutting movement is carried out for the first time (step 602).
  • the counter-cutting movement is carried out by actuating the actuator.
  • This carrying out of the counter-cutting movement by actuating the actuator takes place in particular in a second direction of the actuator that is opposite to the first direction, as designated by B2 in Figure 1a.
  • a step 806 the cutting movement is carried out a second time by actuating the actuator, so that a second sample section is cut from the sample block by means of the knife, for example again by rotating the actuator in the first direction or direction of rotation, as designated by B1 in Figure 1a, for example.
  • the additional adjustment device therefore makes it possible to interrupt the specified movement sequence, to reset the sample block by turning it in the opposite direction, and to follow the specified movement sequence again.
  • This can also be referred to as a so-called short stroke, since a complete run-through of the movement sequence (full stroke) is not necessary.

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

L'invention concerne un agencement de déplacement de coupe (200) pour un microtome, l'agencement de déplacement de coupe (200) possédant l'un ou l'autre parmi un porte-lame ou un porte-échantillon (202), l'agencement de déplacement de coupe (200) comportant un mécanisme de réglage (210) au moyen duquel l'un ou l'autre parmi le porte-lame ou le porte-échantillon (202) peut être déplacé dans un déplacement de restauration (R), et l'agencement de déplacement de coupe (200) comportant un dispositif de réglage supplémentaire (220), différent du mécanisme de réglage (210), et l'agencement de déplacement de coupe (200) étant conçu de telle sorte que, au moyen du dispositif de réglage supplémentaire (220), le mécanisme de réglage (210) peut être invité à réaliser au moins partiellement le déplacement de restauration (R). L'invention concerne également un microtome et un procédé associé.
EP22844233.1A 2022-12-29 2022-12-29 Agencement de déplacement de coupe, microtome et procédé associé Pending EP4643110A1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/EP2022/088032 WO2024141160A1 (fr) 2022-12-29 2022-12-29 Agencement de déplacement de coupe, microtome et procédé associé

Publications (1)

Publication Number Publication Date
EP4643110A1 true EP4643110A1 (fr) 2025-11-05

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Family Applications (1)

Application Number Title Priority Date Filing Date
EP22844233.1A Pending EP4643110A1 (fr) 2022-12-29 2022-12-29 Agencement de déplacement de coupe, microtome et procédé associé

Country Status (4)

Country Link
EP (1) EP4643110A1 (fr)
JP (1) JP2025538886A (fr)
CN (1) CN120359400A (fr)
WO (1) WO2024141160A1 (fr)

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US9032854B2 (en) * 2011-12-21 2015-05-19 Sakura Finetek U.S.A., Inc. Reciprocating microtome drive system
CN207522703U (zh) * 2017-11-30 2018-06-22 徕卡显微系统(上海)有限公司 切片机

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