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EP4151315B1 - Centrifugeuse et procédé de nettoyage d'une centrifugeuse - Google Patents

Centrifugeuse et procédé de nettoyage d'une centrifugeuse Download PDF

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Publication number
EP4151315B1
EP4151315B1 EP22196177.4A EP22196177A EP4151315B1 EP 4151315 B1 EP4151315 B1 EP 4151315B1 EP 22196177 A EP22196177 A EP 22196177A EP 4151315 B1 EP4151315 B1 EP 4151315B1
Authority
EP
European Patent Office
Prior art keywords
rotor
cleaning
cleaning solution
centrifuge
rotor chamber
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP22196177.4A
Other languages
German (de)
English (en)
Other versions
EP4151315A1 (fr
EP4151315C0 (fr
Inventor
Dr. Wolfgang Mann
Frank FEIST
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bluecatbio GmbH
Original Assignee
Bluecatbio GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bluecatbio GmbH filed Critical Bluecatbio GmbH
Priority to EP24213729.7A priority Critical patent/EP4484014A3/fr
Publication of EP4151315A1 publication Critical patent/EP4151315A1/fr
Application granted granted Critical
Publication of EP4151315B1 publication Critical patent/EP4151315B1/fr
Publication of EP4151315C0 publication Critical patent/EP4151315C0/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B15/00—Other accessories for centrifuges
    • B04B15/06—Other accessories for centrifuges for cleaning bowls, filters, sieves, inserts, or the like
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L13/00—Cleaning or rinsing apparatus
    • B01L13/02—Cleaning or rinsing apparatus for receptacle or instruments
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B11/00—Feeding, charging, or discharging bowls
    • B04B11/04—Periodical feeding or discharging; Control arrangements therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B13/00—Control arrangements specially designed for centrifuges; Programme control of centrifuges
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B7/00—Elements of centrifuges
    • B04B7/02—Casings; Lids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
    • B08B3/003—Cleaning involving contact with foam
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
    • B08B3/04—Cleaning involving contact with liquid
    • B08B3/10—Cleaning involving contact with liquid with additional treatment of the liquid or of the object being cleaned, e.g. by heat, by electricity or by vibration
    • B08B3/102—Cleaning involving contact with liquid with additional treatment of the liquid or of the object being cleaned, e.g. by heat, by electricity or by vibration with means for agitating the liquid
    • B08B3/104—Cleaning involving contact with liquid with additional treatment of the liquid or of the object being cleaned, e.g. by heat, by electricity or by vibration with means for agitating the liquid using propellers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
    • B08B9/08—Cleaning containers, e.g. tanks
    • B08B9/0804—Cleaning containers having tubular shape, e.g. casks, barrels, drums
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
    • B08B9/08—Cleaning containers, e.g. tanks
    • B08B9/0821—Handling or manipulating containers, e.g. moving or rotating containers in cleaning devices, conveying to or from cleaning devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
    • B08B9/08—Cleaning containers, e.g. tanks
    • B08B9/20—Cleaning containers, e.g. tanks by using apparatus into or on to which containers, e.g. bottles, jars, cans are brought
    • B08B9/28—Cleaning containers, e.g. tanks by using apparatus into or on to which containers, e.g. bottles, jars, cans are brought the apparatus cleaning by splash, spray, or jet application, with or without soaking
    • B08B9/34—Arrangements of conduits or nozzles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
    • B08B9/08—Cleaning containers, e.g. tanks
    • B08B9/20—Cleaning containers, e.g. tanks by using apparatus into or on to which containers, e.g. bottles, jars, cans are brought
    • B08B9/42—Cleaning containers, e.g. tanks by using apparatus into or on to which containers, e.g. bottles, jars, cans are brought the apparatus being characterised by means for conveying or carrying containers therethrough
    • B08B9/44—Cleaning containers, e.g. tanks by using apparatus into or on to which containers, e.g. bottles, jars, cans are brought the apparatus being characterised by means for conveying or carrying containers therethrough the means being for loading or unloading the apparatus
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B2209/00—Details of machines or methods for cleaning hollow articles
    • B08B2209/08—Details of machines or methods for cleaning containers, e.g. tanks

Definitions

  • the present invention relates to a method for cleaning a centrifuge for cleaning a reaction vessel unit.
  • the EP 937502 A2 describes a method for handling a microtiter plate, whereby the microtiter plate is cleaned by centrifugation.
  • the microtiter plate is placed in the rotary housing via a conveyor belt so that the openings of the microtiter plate are directed away from the axis of rotation.
  • This foam can quickly fill a large part of the volume of the rotor and escape at the door.
  • the foaming material cannot be pumped out well by the pump described either, but rather remains in the rotor chamber or in the drainage channel.
  • the close distance of the rotor to the drainage channel is mainly due to the cylindrical shape of the rotor chamber, which is chosen to generate the desired circulation wind.
  • This centrifuge has a rotor and a rotor chamber in which the rotor is rotatably mounted.
  • a reaction vessel unit is inserted into the centrifuge with its openings facing outwards, so that when the rotor rotates, the are driven out of the respective reaction vessels. This allows the reaction vessels to be cleaned essentially without leaving residue.
  • This centrifuge can have a dispensing unit, wherein the dispensing unit has several dispensing nozzles.
  • the dispensing nozzles are preferably arranged next to one another along a line, wherein this line extends transversely to the direction of movement of the reaction vessel unit when loading or unloading the centrifuge.
  • the nozzles of the dispensing unit are arranged adjacent to an opening for loading and unloading the centrifuge with the reaction vessel unit.
  • CN 102175855 A reveals a fully automatic 360° record washing machine.
  • the rotation axis of this machine runs parallel to the horizontal plane and allows several records to be washed simultaneously in one housing, which increases efficiency and greatly reduces costs.
  • US 4,953,575 relates to a washing device for cuvettes.
  • the cuvettes are placed in a holder in a rotor.
  • the liquid is removed from the cuvettes by rotating the rotor.
  • the disclosed centrifuge housing has an opening at its lowest point through which the removed liquid can leave the housing.
  • JP 2009264927 A discloses a device comprising a drum in which a microplate can be placed.
  • the drum can be loaded with several microtiter plates, which then rotate about a horizontal axis of rotation.
  • the drum is loaded with the microtiter plate in such a way that its openings are directed towards the interior of the drum.
  • the JP 2007/178355 A discloses a system for cleaning printed circuit boards, in which one or more microtiter plates are placed in a rotation unit whose axis of rotation is vertical.
  • the device has several nozzles that can spray the microtiter plates with a cleaning liquid from the outside.
  • a centrifuge with a vertical axis of rotation is disclosed in which the rotor can be cleaned by filling water into the chamber and rotating the rotor.
  • the invention is based on the object of creating a method for cleaning a centrifuge for cleaning a reaction vessel unit, wherein the centrifuge has a rotor and a rotor chamber in which the rotor is rotatably mounted, wherein contamination is to be avoided with the centrifuge and reliable operation is to be possible over the long term.
  • the rotor When the rotor rotates, it can be at least partially immersed in the cleaning solution and this is distributed in the rotor space and/or the inlet is designed in such a way that the cleaning solution is distributed in the rotor space when fed by the rotating rotor.
  • the cleaning solution can come into contact with the rotor and be distributed by the centrifugal forces occurring on the rotor and/or be carried along by the air flow generated by the rotor and thus distributed.
  • the cleaning solution is distributed throughout the entire rotor chamber by partially immersing the rotor in the cleaning solution. It is not necessary to completely fill the entire rotor chamber with the cleaning solution. Therefore, the rotor chamber does not have to be perfectly sealed. Furthermore, in contrast to an arrangement in which the axis of rotation of the rotor runs perpendicular to a base, the cleaning solution is not only distributed radially outwards by centrifugal force without being distributed throughout the entire rotor chamber.
  • the cleaning solution in the rotor chamber is carried upwards by the rotor and evenly distributed in the rotor chamber by the resulting air flow.
  • centrifuges for cleaning a reaction vessel unit are now being used with great success. However, they must be cleaned at regular intervals.
  • the design of the centrifuge according to the invention allows the centrifuge to be cleaned independently or automatically. This means that the centrifuge can be part of an automatic process and can be subjected to a cleaning process from time to time without an operator having to intervene manually. The centrifuge can therefore be cleaned several times in a work cycle lasting several hours, for example, without anyone having to intervene manually. This is a significant advantage over conventional centrifuges for cleaning reaction vessel units.
  • the blocking element can be a valve or a hose clamp that can preferably be operated automatically if at least part of the drain line is designed as a hose.
  • the hose clamp can be provided with an actuator to operate it automatically.
  • the actuator can be designed as an eccentric or as an electric or pneumatic piston mechanism.
  • the inlet line can also be fluidically coupled to a dispensing device integrated in the centrifuge, so that a cleaning solution can be supplied to the inlet line by means of the dispensing device.
  • the dispensing device has the function both of dispensing solutions into reaction vessels of the reaction vessel units and of supplying the cleaning solution to the rotor chamber.
  • the drain can be provided with a suction pump and a siphon, the siphon being designed in such a way that when the suction pump is not activated, a liquid with a fill level below a predetermined fill level remains in the rotor chamber. In this way, by not activating the suction pump, it can be ensured that a cleaning solution present in the rotor chamber remains in the rotor chamber, provided the fill level of the cleaning solution is not above the predetermined fill level.
  • the level of this predetermined fill level is preferably selected in such a way that a rotor immerses itself in the liquid when it rotates and at least partially takes it with it.
  • the siphon thus forms the blocking element with which the drain line is blocked up to a certain fill level, provided the suction pump is not activated.
  • a level sensor can be provided in the rotor chamber to detect the level.
  • the level sensor can be an ultrasonic sensor that scans the surface of the liquid. It is advisable to rotate the rotor into a position so that it does not hinder the measurement.
  • the level can also be formed by one or more temperature sensors that are attached to the inner surface of the housing and are used to measure a specific level of the liquid.
  • the inlet can be arranged above a rotation axis of the rotor so that when the cleaning solution is fed in, it can come into contact with the rotor.
  • the rotor can of course be in a position in which it does not come into contact with the cleaning solution fed via the inlet, e.g. if it is aligned vertically.
  • a cleaning liquid fed in in this way is carried along by a rotating, in particular rapidly rotating rotor and distributed in the rotor space. Low speeds of just a few rpm are sufficient for this.
  • the rotor is rotated at speeds of at least 10 rpm or at least 50 rpm or more.
  • the rotor should not be rotated faster than 100 rpm when the cleaning solution has been introduced into the rotor space up to a predetermined level so that the rotor can be immersed in the cleaning solution. If, however, the cleaning solution is introduced into the rotor chamber, for example by atomization, without the cleaning solution collecting at the bottom of the rotor chamber, then the rotor can also be operated at higher speeds of, for example, at least 100 rpm. In this case, speeds of at least 500 rpm or at least 1000 rpm can also be appropriate.
  • the inlet can also have one or more nozzles to atomize the cleaning solution into the rotor chamber.
  • a cleaning liquid atomized in this way in the rotor chamber can also be evenly distributed in the rotor chamber by rotating the rotor.
  • the cleaning solution When the cleaning solution is removed, the contaminants in the rotor chamber are removed.
  • the cleaning solution can flow out via the drain and thus be removed from the rotor chamber. This can be controlled, for example, by opening a blocking element in a drain line.
  • the rotor in such a centrifuge has two functions. On the one hand, the rotor is used to empty the reaction vessel units by spinning the contents out of the individual reaction vessels of the reaction vessel units by rotating the rotor. On the other hand, the rotor is also used to distribute the cleaning solution in the rotor chamber, thus ensuring even and reliable cleaning of the rotor chamber.
  • the cleaning solution can be distributed on the one hand by the rotor being at least partially immersed in the cleaning solution as it rotates and taking it with it. However, the cleaning solution can also be supplied in such a way that it is taken directly by the rotor or by the air current generated by the rotor and distributed in the rotor chamber. This is particularly true if the cleaning solution is atomized in the rotor chamber; then a mist of the cleaning solution is evenly distributed in the rotor chamber by rotating the rotor.
  • the cleaning solution can be a non-foaming cleaning solution, which contains, for example, formaldehyde or paraformaldehyde.
  • a non-foaming cleaning solution can drain out of the rotor chamber independently, without any further activities.
  • the rotation of the rotor can serve to drive the cleaning solution to the drain and remove it from the rotor chamber.
  • the cleaning solution can also drain automatically when the rotor is at a standstill if the drain line is unlocked accordingly.
  • a centrifuge 1 for the method according to the invention comprises a rotor 2, a housing 3, a drive device 4 for rotating the rotor 2 about a rotation axis 5.
  • the rotor has at least one receiving area 6 for receiving a reaction vessel unit 7.
  • the reaction vessel unit 7 is usually a microtiter plate.
  • Such microtiter plates can be designed with a different number of reaction vessels. Microtiter plates with six to 4096 reaction vessels are common, with microtiter plates with 96, 384 or 1536 reaction vessels being the most common versions. In microtiter plates with 384 or 1536 reaction vessels, the individual reaction vessels are so thin that a liquid normally adheres to them solely due to capillary forces, so that even when arranging such a Microtiter plate with its openings facing downwards the liquid does not flow out. This does not apply to microtiter plates with fewer reaction vessels, each of which is larger.
  • Such a reaction vessel unit 7 can be inserted alone into a receiving area 6 or on a carrier unit.
  • a carrier unit is used which has a coupling element which can be coupled to a loading and unloading device 8.
  • a loading and unloading device is for example in the DE 10 2016 101 163 described. It is explained in more detail below.
  • the housing 3 delimits a rotor chamber 9.
  • the area of the housing 3 delimiting the rotor chamber 9 is formed from a lower shell 10, upper shell 11, front end wall 12 and rear end wall 13. Further parts of the housing, which are not shown in the attached figures, are connected to the rear end wall.
  • a ball bearing 14 in which a continuous shaft 15 of the rotor 2 is rotatably mounted.
  • the center line of the shaft 15 forms the axis of rotation 5.
  • the axis of rotation 5 runs parallel to a base surface 16 which is formed by the underside of the lower shell 10.
  • the rear end of the shaft 15 is coupled to the drive device 4.
  • the further part of the housing which is connected to the rear of the housing, contains the drive device 17, the loading and unloading device 8 and a central control device (not shown) with which all components of the centrifuge 1 are controlled.
  • a balcony 18 is attached to the outside of the front end wall 12 and serves to accommodate a reaction vessel unit 7.
  • a loading and unloading opening 19 is formed in the front end wall 12 through which a reaction vessel unit 7 can be inserted into the rotor chamber 9 and pushed out again.
  • the loading and unloading opening 19 is provided with a pivoting door 20 so that the rotor chamber can be closed.
  • a dispensing unit 39 with several dispensing nozzles 40 and/or an optical detection unit, in particular in the form of a line camera, can be provided.
  • the loading and unloading device 8 has a sliding rod (not shown) which can be moved with its free end horizontally through the rotor chamber 9 through a through opening 21 in the rear end wall 13.
  • the loading and unloading device 8 has a linear drive for this purpose, so that the sliding rod can be moved along its longitudinal direction.
  • the sliding rod has a coupling element at its free end which can be coupled to a corresponding coupling element on the carrier unit or to a reaction vessel unit 7, so that the carrier unit with a reaction vessel unit or the reaction vessel unit can be moved directly by moving the sliding rod from the balcony 18 through the loading and unloading opening 19 into the rotor chamber 9, the rotor 2 being arranged with a receiving area 6 adjacent to the loading and unloading opening 19, so that the carrier unit or the reaction vessel unit is moved into the receiving area 6 of the rotor 2.
  • the coupling between the sliding rod and the carrier unit or the reaction vessel unit 7 can be released so that the carrier unit or the reaction vessel unit is freely movable in the rotor 2 and the rotor can be rotated accordingly with this unit.
  • the carrier unit or reaction vessel unit 7 can be pushed from the receiving area 6 of the rotor 2 through the loading and unloading opening 19 back onto the balcony 18.
  • the reaction vessel unit 7 can be removed from the balcony 18, for example by means of a robot.
  • the lower shell 10 has a groove 22 which runs approximately parallel to the axis of rotation 5.
  • the groove 22 extends from the rear end wall 13 to the area of the front end wall 12, whereby it is inclined or sloping towards the front ( Figure 4a ).
  • An outlet opening 23 is formed on the front of the lower bowl 10, into which the channel 22 opens.
  • a connection pin 24 to which a hose 25 can be connected is arranged on the outlet opening 23.
  • the hose 25 usually opens into a receiving container (not shown) in which the liquids are collected which are spun out of the reaction vessels of the reaction vessel unit 7 in the centrifuge 1.
  • the container preferably has a ventilation opening or the hose passes through the container with some play so that liquid flowing out of the centrifuge through the hose 25 does not create any counterpressure in the container.
  • the lower shell 10 has inner surfaces adjacent to the channel 22, which extend from an upper edge of the channel 22 in an obliquely rising direction outwards ( Fig. 2 ). These inner surfaces thus form a funnel 26 and are referred to below as funnel surfaces 27.
  • the funnel surfaces 27 are inclined at an angle of approximately 30° to 60° relative to the horizontal. Essentially planar means that the funnel surfaces have a radius of curvature of more than 0.5 m and preferably more than 1 m. In the present embodiment, the funnel surfaces 27 extend laterally in the direction beyond the area of the rotor 2, even when it is in its horizontal position.
  • the inner surfaces of the lower shell 10 extend approximately vertically upwards from the outer edge of the funnel 26 or the funnel surfaces 27. They thus form vertical surfaces 28.
  • the upper shell 11 is attached to the upper edge of the lower shell 10 and has a channel-like shape with a semicircular cross-section.
  • the inner surface of the upper shell 11 is flush with the vertical surface 28.
  • the cross-section of the housing 3 is therefore not cylindrical, but only has a cylindrical curvature in the upper region of the shell 11, whereas the lower shell 10 has a funnel-shaped cross-section and ends in the channel 22.
  • the channel 22 is slightly offset downwards from the funnel-shaped lower shell 10 and has two approximately vertically arranged side walls 37a, 37b.
  • the channel itself is designed with an incline so that any liquid contained therein drains away.
  • the lower shell 10 and the upper shell 11 are made of metal.
  • the inner surfaces of the lower shell 10 and the upper shell 11 are coated with a smooth plastic layer so that liquids that are ejected from the reaction vessels of the reaction vessel units 7 run quickly along the inner surfaces, are guided from the funnel 26 to the channel 22 and exit from the rotor chamber 9.
  • the plastic layer is made of PTFE.
  • the upper edge of the channel 22 is spaced from the rotation axis 5 by at least 1.32 times the maximum radius of the rotor 2. This creates a free space in the funnel 26 which is not touched by the rotor 2 during one revolution. Liquid can collect in this free space.
  • a maximum level 29 of the liquid is shown which can collect in the funnel 26 without coming into contact with the rotor. This makes it possible, in the case of large-volume reaction vessels of a reaction vessel unit 7, to empty the majority of the liquid contained therein at once, to collect it in the funnel 26 so that it can gradually flow out through the outlet opening 23.
  • the air flow generated by the rotor when it rotates is at its lowest in this area, so that liquid can settle at the bottom of the funnel, i.e. in the channel 22, and flow out of the channel 22 through the outlet opening 23. Due to the low flow velocity, the risk of liquids located in the funnel-shaped area adjacent to the channel 22 being driven upwards by the air flow is also low.
  • This design of the funnel 26 and the channel 22 eliminates the need for a suction pump.
  • the dispensing unit 30, which can also be referred to as a dispensing head, has several of the dispensing nozzles 31, which are arranged along a straight line and with their openings pointing downwards.
  • the dispensing unit 30 is connected to a reagent line 32, via which reagents are fed to the dispensing unit 30, which are then distributed downwards via the individual dispensing nozzles 31.
  • the dispensing unit basically has the WO 2018/234420 A1 known function that reaction vessels of a reaction vessel unit 7 can be filled with reagents when the reaction vessel unit 7 is moved past the dispensing unit 30 by means of the loading and unloading device 8.
  • the balcony 18 is formed in the area below the dispensing unit 30 with an upwardly open channel 33 in which the reagents dispensed by the dispensing nozzles 31 are collected if no reaction vessel unit 7 is arranged below the dispensing nozzles 31, as in Figure 4a is shown.
  • the channel 33 is connected in a communicating manner to a collecting hose 34, so that the reagents collected in the channel 33 flow away via the collecting hose 34.
  • the collecting hose 34 opens into the hose 25 at a branch 35. With respect to the rotor chamber 9, starting from the branch 35, the collecting hose forms an inlet line and the hose 25 forms an outlet line for draining liquids from the rotor chamber 9.
  • a blocking element 36 is arranged in the hose 25 downstream of the branch 35, with which the passage of the hose 25 can be blocked.
  • the blocking element 36 can be a preferably electrically actuated valve in order to open or close the passage of the hose.
  • the blocking element can also be a hose clamp, which can be opened or closed, for example, with an actuator or by means of an eccentric.
  • the blocking element 36 blocks the passage of the hose 25 and a cleaning solution is supplied with the dispensing unit 30 via the collecting hose 34, the cleaning solution flows via the hose 25 and the outlet opening 23 into the rotor chamber 9.
  • the outlet opening 23 then serves as the inlet for the cleaning solution.
  • the rotor chamber 9 is filled to above the level 29 ( Figure 2 ) is filled with cleaning solution, so that when the rotor 2 rotates, it is immersed in the cleaning solution and takes part of the cleaning solution with it and distributes it in the rotor chamber 9.
  • the rotor chamber 9 is filled at least up to a level 43, as Figure 2
  • the level 43 is approximately 5% of the radius of the rotor 2 and preferably at least 10% of the radius of the rotor 2 above the level 29 which is just not touched when the rotor 2 rotates.
  • the cleaning solution is distributed in the rotor chamber 9 so that all parts of the rotor chamber 9 come into contact with the cleaning solution.
  • cleaning solution can continue to be supplied via the dispensing unit 30 in order to slow down or prevent the level of the cleaning solution from dropping.
  • the rotation of the rotor can be adjusted or the rotor can be rotated further in order to cause a continuous swirling of the cleaning solution in the rotor chamber by the air current.
  • the blocking element 36 is opened so that the cleaning solution flows out through the outlet opening 23. This can be supported by further rotation of the rotor so that the cleaning solution is driven into the channel 22.
  • This cleaning process of the rotor chamber 9 can be carried out fully automatically and is controlled by the central control device.
  • a non-foaming cleaning solution such as formaldehyde or paraformaldehyde, is preferably used as the cleaning solution, with which the entire rotor chamber 9 can be reliably disinfected.
  • the cleaning solution contains surfactants, which cause the cleaning solution to foam when the rotor is rotated. Foaming of the cleaning solution causes a very rapid and even distribution of the cleaning solution in the rotor chamber 9, which is why the rotational speed and/or duration at which the rotor is rotated in the rotor chamber 9 can or should be significantly reduced compared to the distribution of non-foaming cleaning solution.
  • a foam-reducing solution is fed to the rotor chamber 9 via the dispensing unit 30 and the collecting hose 34 and distributed by rotating the rotor 2.
  • foam-reducing solution can contain alcohol, for example.
  • a solution containing alcohol also has the advantage that it evaporates very quickly and the rotor chamber 9 therefore dries correspondingly quickly.
  • a second embodiment of the centrifuge 1 ( Figure 4b ) is designed essentially in the same way as the first embodiment, unless explained otherwise below, which is why identical parts are given the same reference numerals and are not explained again.
  • the second embodiment does not have to have a dispensing unit.
  • an atomizing nozzle 40 is arranged in the feed opening 39, with which reagents supplied via the reagent line 32 are atomized into the rotor chamber 9.
  • a cleaning solution By supplying a cleaning solution via the feed opening 39, this is introduced into the rotor chamber 9 and atomized by the atomizing nozzle 40 into a mist, which is evenly distributed in the rotor chamber 9 by rotating the rotor 2. A portion of the cleaning solution settles in the channel 22 and flows out of the rotor chamber 9 via the outlet opening 23 and the hose 25. This allows cleaning solution to be continuously circulated in the rotor chamber 9 and drained away in order to remove contamination from the rotor chamber 9.
  • a blocking element 36 can optionally be provided in the hose 25 in order to block the passage of the hose 25 and to retain cleaning solution in the rotor chamber 9.
  • non-foaming cleaning solutions or foaming cleaning solutions can also be supplied. If foaming cleaning solutions are used, it is expedient, just as in the first embodiment, to supply a foam-degrading solution to the rotor chamber 9 in order to remove the foaming cleaning solution from the rotor chamber 9.
  • the cleaning solution or cleaning solutions can be supplied to or removed from the rotor chamber 9 in different ways in order to clean the rotor chamber 9.
  • the rotor 2 which is present in the centrifuge 1, is used to distribute the cleaning solution evenly in the rotor chamber 9.
  • the rotational speed and the duration at which the rotor 2 is rotated is adapted to the geometry of the rotor interior 9 and the behavior of the cleaning solution accordingly.
  • it can be particularly expedient (regardless of the structural design of the centrifuge) to rotate the rotor 2 at least once clockwise and at least once anti-clockwise in order to obtain the most even distribution of the cleaning solution in the rotor chamber 9.
  • one or more atomizing nozzles 40 it is expedient to supply the cleaning solution under pressure so that the atomizing nozzles 40 cause efficient atomization of the cleaning solution.
  • the supply and even distribution as well as the removal of the cleaning solution from the rotor chamber 9 can be carried out fully automatically.
  • the centrifuge 1 can be used in an automatic production process in which many reaction vessel units 7 are repeatedly cleaned, ensuring in the long term that no contamination occurs from one reaction vessel unit 7 to another reaction vessel unit 7.
  • the intervals of the cleaning processes of the rotor chamber 9 must be adapted to the amount and reactivity of the reagents contained in the reaction vessel units 7.
  • Such a cleaning process can, for example, be carried out at an interval of no more than 10 minutes or no more than 60 minutes. With less reactive reagents and small quantities, however, it can also be expedient to carry out such a cleaning process only once a day.
  • agents can be used as solvents that destroy nucleic acids and thus exclude contamination.
  • agents are, for example, perchlorate, strong oxidizing agents and/or enzymes such as DNAses.
  • the system can be completely cleaned without having to open the interior or the device.
  • the invention can be briefly summarized as follows:
  • the invention relates to a method for cleaning a centrifuge 1.
  • the centrifuge 1 has a rotor 2 and a rotor chamber 9 in which the rotor 2 is arranged and rotatably mounted, wherein the rotor 2 has a receiving area 6 for receiving the reaction vessel unit 7.
  • the rotor chamber 9 is delimited by a housing 3, wherein the housing 3 has a drain to to drain liquid discharged from the reaction vessels and is provided with an inlet to fill the rotor chamber 9 with a cleaning solution in such a way that when the rotor 2 rotates, it is at least partially immersed in the cleaning solution and distributes it in the rotor chamber 9 and/or the inlet is designed such that the cleaning solution is distributed in the rotor chamber 9 when fed by the rotating rotor 2.

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  • Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Centrifugal Separators (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Cleaning By Liquid Or Steam (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Claims (11)

  1. Procédé de nettoyage d'une centrifugeuse (1) pour le nettoyage d'une unité de cuve de réaction (7), la centrifugeuse (1) comprenant un rotor (2) et une chambre de rotor (9) dans laquelle le rotor (2) est disposé et monté de manière rotative, le rotor (2) comprenant une région de réception (6) pour recevoir l'unité de cuve de réaction (7), l'axe de rotation (5) du rotor (2) s'étendant parallèlement à une empreinte (16), et la chambre du rotor (9) étant délimitée par un boîtier (3), le boîtier (3) comprenant une sortie pour l'évacuation d'un liquide déchargé des cuves de réaction et étant pourvu d'une entrée, et les étapes suivantes étant effectuées :
    - la chambre du rotor (9) est remplie d'une solution de nettoyage au moins jusqu'à un niveau prédéterminé (43) de manière à ce que le rotor (2) soit au moins partiellement immergé dans la solution de nettoyage pendant sa rotation,
    - le rotor (2) est mis en rotation, ce qui entraîne la distribution de la solution de nettoyage dans la chambre du rotor (9), et
    - la solution de nettoyage est retirée de la chambre du rotor (9).
  2. Le procédé de nettoyage d'une centrifugeuse (1) selon la revendication 1,
    caractérisé en ce que
    la sortie du boîtier (3) constitue également l'entrée.
  3. Le procédé de nettoyage d'une centrifugeuse (1) selon la revendication 2,
    caractérisé en ce que
    une ouverture dans le boîtier (3), qui forme la sortie et l'entrée, est reliée à une conduite de fluide qui comprend une branche et se ramifie en une conduite d'entrée et une conduite de sortie, la conduite de sortie étant adaptée pour évacuer un fluide et la conduite d'entrée étant adaptée pour fournir un fluide, et la conduite de sortie comprenant un élément de blocage (36) pour bloquer la conduite de sortie.
  4. Procédé de nettoyage d'une centrifugeuse (1) selon la revendication 3,
    caractérisé en ce que
    la conduite d'entrée est couplée fluidiquement à un dispositif de distribution intégré à la centrifugeuse (1), de sorte qu'une solution de nettoyage peut être fournie à la conduite d'entrée au moyen du dispositif de distribution.
  5. Le procédé de nettoyage d'une centrifugeuse (1) selon l'une quelconque des revendications 1 à 4,
    caractérisé en ce que
    la sortie comprend une pompe d'aspiration et un siphon (41), le siphon (41) étant adapté de telle sorte que, lorsque la pompe d'aspiration n'est pas actionnée, un liquide dont le niveau de remplissage est inférieur à un niveau de remplissage prédéterminé reste dans la chambre du rotor (9).
  6. Le procédé de nettoyage d'une centrifugeuse (1) selon l'une quelconque des revendications 1 à 5,
    caractérisé en ce que
    un capteur de niveau de remplissage est prévu pour détecter le niveau de remplissage dans la chambre du rotor (9).
  7. Le procédé de nettoyage d'une centrifugeuse (1) selon l'une quelconque des revendications 1 à 6,
    caractérisé en ce que
    l'entrée est disposée au-dessus d'un axe de rotation du rotor (2), de sorte que la solution de nettoyage peut entrer en contact avec le rotor (2) lorsqu'elle est alimentée.
  8. Le procédé de nettoyage d'une centrifugeuse (1) selon l'une quelconque des revendications 1 à 7,
    caractérisé en ce que
    l'entrée comprend une ou plusieurs buses pour pulvériser la solution de nettoyage vers la chambre du rotor (9).
  9. Le procédé de nettoyage d'une centrifugeuse (1) selon l'une quelconque des revendications 1 à 8,
    caractérisé en ce que
    la solution de nettoyage est une solution de nettoyage non moussante qui contient du formaldéhyde ou du paraformaldéhyde, par exemple.
  10. Le procédé de nettoyage d'une centrifugeuse (1) selon l'une quelconque des revendications 1 à 8,
    caractérisé en ce que
    la solution de nettoyage est une solution de nettoyage moussante, en particulier une solution de nettoyage contenant des agents de surface, et pour éliminer la solution de nettoyage, une solution de dégradation de mousse contenant de l'alcool, par exemple, est introduite dans la chambre du rotor (9).
  11. Le procédé selon la revendication 10,
    caractérisé en ce que
    pendant ou après l'alimentation de la solution de dégradation de mousse, le rotor (2) est mis en rotation afin de répartir la solution de dégradation de mousse dans l'espace du rotor (9).
EP22196177.4A 2021-09-16 2022-09-16 Centrifugeuse et procédé de nettoyage d'une centrifugeuse Active EP4151315B1 (fr)

Priority Applications (1)

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EP24213729.7A EP4484014A3 (fr) 2021-09-16 2022-09-16 Centrifugeuse et procédé de nettoyage d'une centrifugeuse

Applications Claiming Priority (1)

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DE102021124023.9A DE102021124023B4 (de) 2021-09-16 2021-09-16 Zentrifuge und Verfahren zum Reinigen einer Zentrifuge

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EP4151315A1 EP4151315A1 (fr) 2023-03-22
EP4151315B1 true EP4151315B1 (fr) 2024-11-20
EP4151315C0 EP4151315C0 (fr) 2024-11-20

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EP22196177.4A Active EP4151315B1 (fr) 2021-09-16 2022-09-16 Centrifugeuse et procédé de nettoyage d'une centrifugeuse

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US (1) US12383907B2 (fr)
EP (2) EP4484014A3 (fr)
JP (1) JP2023047313A (fr)
CN (1) CN115805145A (fr)
DE (1) DE102021124023B4 (fr)

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DE102017113583A1 (de) * 2017-06-20 2018-12-20 Bluecatbio Gmbh Zentrifuge
DE102022102705A1 (de) 2022-02-04 2023-08-10 BlueCat Solutions GmbH Auffangvorrichtung für eine Reaktionsgefäßeinheit und Reaktionsgefäßeinheit
DE102022102701A1 (de) 2022-02-04 2023-08-10 Bluecatbio Gmbh Ableitvorrichtung für eine Reaktionsgefäßeinheit, Zentrifuge und Verfahren zum Reinigen einer Reaktionsgefäßeinheit
DE102023120681A1 (de) 2023-08-03 2025-02-06 Bluecatbio Gmbh Ableitvorrichtung für das Zentrifugieren einer Reaktionsgefäßeinheit, Zentrifuge und Verfahren zum Reinigen einer Ableitvorrichtung
DE202023105780U1 (de) 2023-10-05 2025-01-28 Bluecatbio Gmbh Rückhaltevorrichtung für eine Reaktionsgefäßeinheit
DE202023105800U1 (de) 2023-10-06 2025-01-28 Bluecatbio Gmbh Magnetträger für Reaktionsgefäßeinheiten
DE202023105801U1 (de) 2023-10-06 2025-01-28 Bluecatbio Gmbh Wuchtkörper

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Also Published As

Publication number Publication date
DE102021124023A1 (de) 2023-03-16
EP4151315A1 (fr) 2023-03-22
EP4151315C0 (fr) 2024-11-20
US12383907B2 (en) 2025-08-12
EP4484014A2 (fr) 2025-01-01
EP4484014A3 (fr) 2025-03-19
JP2023047313A (ja) 2023-04-05
DE102021124023B4 (de) 2025-07-17
US20230077651A1 (en) 2023-03-16
CN115805145A (zh) 2023-03-17

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