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EP1860060B1 - Appareil pour mélanger des micro-gouttelettes - Google Patents

Appareil pour mélanger des micro-gouttelettes Download PDF

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Publication number
EP1860060B1
EP1860060B1 EP06010516A EP06010516A EP1860060B1 EP 1860060 B1 EP1860060 B1 EP 1860060B1 EP 06010516 A EP06010516 A EP 06010516A EP 06010516 A EP06010516 A EP 06010516A EP 1860060 B1 EP1860060 B1 EP 1860060B1
Authority
EP
European Patent Office
Prior art keywords
carrier
carriers
microdroplets
hydrophilic surface
another
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Not-in-force
Application number
EP06010516A
Other languages
German (de)
English (en)
Other versions
EP1860060A1 (fr
Inventor
Ulrich Dr. Rer. Nat. Sieben
Dr. Klapproth Holger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
TDK Micronas GmbH
Original Assignee
TDK Micronas GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by TDK Micronas GmbH filed Critical TDK Micronas GmbH
Priority to DE502006007372T priority Critical patent/DE502006007372D1/de
Priority to EP06010516A priority patent/EP1860060B1/fr
Priority to US11/805,242 priority patent/US20090017505A1/en
Publication of EP1860060A1 publication Critical patent/EP1860060A1/fr
Application granted granted Critical
Publication of EP1860060B1 publication Critical patent/EP1860060B1/fr
Not-in-force legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0241—Drop counters; Drop formers
    • B01L3/0262—Drop counters; Drop formers using touch-off at substrate or container
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/302—Micromixers the materials to be mixed flowing in the form of droplets
    • B01F33/3021—Micromixers the materials to be mixed flowing in the form of droplets the components to be mixed being combined in a single independent droplet, e.g. these droplets being divided by a non-miscible fluid or consisting of independent droplets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/3035—Micromixers using surface tension to mix, move or hold the fluids
    • B01F33/30351—Micromixers using surface tension to mix, move or hold the fluids using hydrophilic/hydrophobic surfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5088—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above confining liquids at a location by surface tension, e.g. virtual wells on plates, wires
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/90—Heating or cooling systems
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/143—Quality control, feedback systems
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/56—Labware specially adapted for transferring fluids
    • B01L3/563—Joints or fittings ; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors

Definitions

  • the invention relates to a device for mixing microdroplets according to the preamble of claim 1.
  • EP 0 075 605 A1 (which discloses a device according to the preamble of claim 1) and US 2005/0019224 A1 are known devices for mixing microdroplets, which have two carriers, whose surfaces are each structured such that hydrophilic surface areas are separated by hydrophobic surface areas.
  • the carriers are positioned so close to one another by means of a positioning device with the structured surfaces facing one another that microdroplets which can be applied to the hydrophilic surface regions of the one carrier come into contact with microdroplets which can be applied to the hydrophilic surface regions of the other carrier.
  • a device for mixing microdroplets which has a first and a second carrier.
  • the surface of the first carrier is structured in such a way that hydrophilic surface regions which are closely adjacent to one another are separated from one another by a hydrophobic surface region.
  • the carriers can be positioned so close to one another that microdroplets which can be applied to the hydrophilic surface regions of the first carrier come into contact with the second carrier and with one another.
  • a device for mixing microdroplets which has a first carrier which is structured in such a way that closely adjacent first hydrophilic surface regions are separated from one another by a first hydrophobic surface region.
  • the surface of a second carrier is structured such that a second hydrophilic surface region is separated from a second hydrophobic surface region.
  • a device for mixing microdroplets which has two carriers whose surfaces are each structured such that a receiving area for a microdroplet is separated from a hydrophobic surface area.
  • the carriers are positioned by means of a positioning with the structured surfaces facing each other so close together that fall into contact with the microdroplets.
  • the device has at least three of the structured carriers, and if these carriers are selectively or alternately positionable by means of the positioning device. This makes it possible, in particular, to mix a plurality of microdroplets in succession, for example to mix first two microdroplets A and B into a microdroplet AB and then this with a microdroplet C to form a microdroplet ABC.
  • At least one carrier has a metal oxide or semimetal oxide substrate which is coated on the hydrophilic surface regions with a polymer having at least one reactive group.
  • the substrate can then be mass produced with high precision by methods of semiconductor manufacturing.
  • the positioning device on the carriers to be positioned to each other on each other cooperating centering, in particular centering bevels.
  • the carriers can then be easily positioned with their surface structuring in a predetermined position relative to each other.
  • a centering can be provided on one carrier a projection and on the other carrier a matching recess.
  • the centering elements can also be optical markers, such as e.g. Crosshairs be brought to coincide with the positioning of the carrier.
  • At least one carrier has a cooling and / or heating element, in particular a Peltier element.
  • the device can then be used to perform a polymerase chain reaction (PCR).
  • PCR polymerase chain reaction
  • An in Fig. 1 to 3 1 for mixing microdroplets 2a, 2b has two approximately plate-shaped carriers 3a, 3b, whose surfaces 4a, 4b are each structured such that a plurality of hydrophilic surface regions 5a, 5b are laterally spaced from one another by a hydrophobic surface region 6a, 6b bounding them.
  • the hydrophilic surface regions 5a, 5b are arranged in a matrix-like manner in a plurality of rows and columns.
  • the matrices of the two Carrier 3a, 3b are designed such that the hydrophobic surface regions 6a, 6b of a first carrier 3a can be used with those of a second carrier 3b to cover, when the carrier 3a, 3b with their hydrophilic surface regions 5a, 5b positioned facing each other.
  • the carrier has crosshairs formed optical position markers, which are arranged in a predetermined position relative to the hydrophilic surface regions 5a, 5b.
  • the supports 3a, 3b each consist of a semiconductor material, e.g. Silicon, which has on its surface a not shown in the drawing fluoropolymer layer which forms the hydrophobic surface region 6a, 6b.
  • a polymer hydrogel is applied, which may have reactive groups.
  • First microdroplets 2a are applied to the hydrophilic surface regions 5a of the first carrier 3a.
  • the carrier 3a may be immersed in, for example, a liquid and then withdrawn therefrom at a rate chosen to adhere the liquid only to the hydrophilic surface regions 5a.
  • the microdroplets 2a can also be applied in any other way to the hydrophilic surface regions 5a, e.g. with the help of a needle, a pipette or by printing, in particular by means of a jet printer.
  • the different surface regions 5a, 6a cause the microdroplets 2a to align themselves in such a way that they are arranged only on the hydrophilic surface regions 5a.
  • Second microdroplets 2b are applied in a corresponding manner to the hydrophilic surface regions 5a, 5b of the second carrier 3b. Then, the carriers 3a, 3b are positioned with their extension planes parallel to each other such that the hydrophilic surface regions 5a of the first carrier 3a are mirror images of the hydrophilic surface regions 5b of the second carrier 3b. For the correct alignment of the carriers 3a, 3b, the position marks 7 of the one carrier 3a are brought into coincidence with the position marks 7 of the other carrier 3b.
  • the carriers 3a, 3b are initially spaced so far apart that the microdroplets 2a, 2b do not touch.
  • the microdroplets 2a of the upper side of the first carrier 3a and the microdroplets 2b are arranged on the underside of the second carrier 3b.
  • the last-mentioned microdroplets adhere to the hydrophilic surface regions 5b against the force of gravity.
  • the plate assembly formed from the carriers 3a, 3b in a another position in the room is positioned, for example, 90 ° to a normal to the plane in FIGS. 2 and 3 turning axis turned ..
  • the carriers 3a, 3b are moved toward one another by means of a positioning device, not shown in detail in the drawing, normal to their extension planes until the microdroplets 2a located on the surface of the first carrier 3a each have a corresponding thereto Touch microdroplets 2b of the second carrier 3b and each mix with this, for example, to start a chemical reaction between the different liquids of the microdroplets 2a, 2b and / or dissolved therein substances.
  • Fig. 3 It can be seen that the carriers 3a, 3b after the mixing of the microdroplets 2a, 2b to a new microdroplet 2 are spaced apart by a narrow gap such that the microdroplets 2 are spaced from each other by the hydrophobic surface regions 6a, 6b. Microdroplets 2a, 2b, which were arranged on the same carrier 3a, 3b, therefore do not mix.
  • the positioning device has a first housing part 8a connected to the first carrier 3a and a second housing part 8b connected to the second carrier 3b.
  • the first housing part 8a has a receiving recess and the second housing part 8b has a mating projection.
  • inclined surfaces 9 are arranged, which act together with inserted into the first housing part 8a second housing part, that the housing parts 8a, 8b are centered in a predetermined position relative to each other.
  • the housing parts 8a, 8b are preferably made of an inert plastic, with which the carrier parts 3a, 3b are partially encapsulated.
  • hydrophilic surface regions 5a which are separated from one another by a hydrophobic surface region 6a, are provided only on the surface of the first carrier part 3a.
  • the hydrophilic surface regions 5a are arranged in a matrix-like manner in a plurality of rows and columns.
  • Fig. 5 It can clearly be seen that in each case two surface regions 5a are associated with each other in pairs and have a smaller distance from one another than with the remaining hydrophilic surface regions 5a.
  • the surface of the second, serving as a stamp carrier part 3b is completely hydrophilic.
  • a first microdroplet 2a and a second microdroplet 2b on the other surface area 5b are used up on a respective surface area 5a of the paired surface regions 5a.
  • the application of the microdroplets 2a, 2b can be effected for example by printing.
  • the carriers 3a, 3b are arranged with their extension planes parallel to each other, wherein the carriers 3a, 3b are initially spaced apart so far that the second carrier 3b does not touch the microdroplets 2a, 2b located on the first carrier 3a. Then the carriers are moved towards each other approximately normal to their planes of extent until the second carrier 3b touches the pairwise associated microdroplets 2a, 2b and these come into contact with each other.
  • Fig. 8 It can be seen that the carriers 3a, 3b after the mixing of the microdroplets 2a, 2b to a new microdroplet 2 are spaced apart by a narrow gap such that the microdroplets 2 are spaced from each other by the hydrophobic surface region 6a. Thus, only the pairwise associated microdroplets 2a, 2b are mixed together.
  • the arrangement of the surface areas 5a, 6a corresponds to that in FIG Fig. 5 ,
  • second hydrophilic surface regions 5b which are spaced apart from each other by a second hydrophobic surface region 6b, are provided on the second carrier.
  • the first hydrophilic surface regions 5a are as in the embodiment in Fig. 5 coated with first and second microdroplets 2a, 2b.
  • Third microdroplets 2c are applied to the second hydrophilic surface regions 5b.
  • Fig. 9 it can be seen that the carriers 3a, 3b are arranged with their extension planes parallel to one another such that the second hydrophilic surface regions 5b each have one between the first hydrophilic surface regions 5a overlap region of the first hydrophobic surface region 6a.
  • the third microdroplet 2c is arranged in the plan view of the extension planes of the carriers 3a, 3b between a first microdroplet 5a associated therewith and a second microdroplet 5b.
  • the carriers 3a, 3b are initially spaced apart from one another such that the microdroplets 2a, 2b, 2c do not touch each other. Then, the carriers are moved toward each other approximately normal to their planes of extent until the third microdroplets 2c touch and mix with the first and second microdroplets 2a, 2b.
  • Fig. 10 It can be seen that the carriers 3a, 3b after the mixing of the microdroplets 2a, 2b, 2c to a new microdroplet 2 by a narrow gap are spaced apart from each other such that the microdroplets 2 are spaced from each other by the hydrophobic surface region 6a, 6b. Thus, only three mutually associated microdroplets 2a, 2b, 2c are mixed together.
  • the first microdroplet 2a may contain hydrogen peroxide, the second microdroplet 2b luminol, and the third microdroplet a serum to be assayed, in which ligands are reacted with an enzymatic marker, e.g. Horseradish peroxidase (HRP) are labeled.
  • an enzymatic marker e.g. Horseradish peroxidase (HRP) are labeled.
  • At least one of the carriers 3a, 3b has at a hydrophilic surface area a moisture and / or conductivity sensor not shown in the drawing.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Claims (6)

  1. Dispositif (1) destiné au mélange de microgouttes (2a, 2b, 2c) et présentant
    au moins deux supports (3a, 3b) dont les surfaces (4a, 4b) sont structurées de telle sorte qu'au moins une partie hydrophile de surface (5a, 5b) soit entourée par au moins une partie hydrophobe de surface (6a, 6b),
    un dispositif de positionnement au moyen duquel les supports (3a, 3b) peuvent être placés étroitement l'un contre l'autre avec les surfaces structurées (4a, 4b) tournées l'une vers l'autre de telle sorte que des microgouttes (2a, 2b, 2c) qui peuvent être placées sur les parties hydrophiles de surface (5a, 5b) entrent en contact mutuel,
    au moins deux supports (3a, 3b), la surface (4a) d'un premier support (3a) étant structurée de telle sorte que des parties hydrophiles de surface (5a) étroitement voisines l'une de l'autre soient séparées l'une de l'autre par au moins une partie hydrophobe de surface (6a) et
    un dispositif de positionnement au moyen duquel les supports (3a, 3b) peuvent être placés étroitement l'un contre l'autre de telle sorte que des microgouttes (2a, 2b, 2c) qui peuvent être placées sur les parties hydrophiles de surface (5a) du premier support (3a) peuvent être mises en contact avec un deuxième support (3b) et les unes avec les autres ou
    au moins deux supports (3a, 3b), la surface (4a) d'un premier support (3a) étant structurée de telle sorte que des premières parties hydrophiles de surface (5a) étroitement voisines l'une de l'autre soient séparées l'une de l'autre par au moins une première partie hydrophobe de surface (6a), la surface (4b) d'un deuxième support (3b) étant structurée de telle sorte qu'au moins une deuxième partie hydrophile de surface (5b) soit entourée par au moins une deuxième partie hydrophobe de surface (6b) et
    un dispositif de positionnement au moyen duquel les supports (3a, 3b) peuvent être placés étroitement l'un contre l'autre avec leurs surfaces structurées (4a, 4b) tournées l'une vers l'autre de telle sorte que la deuxième partie hydrophile de surface (5b) recouvre une première partie hydrophobe de surface (6a) située entre les premières parties hydrophiles de surface (5a) et que des microgouttes (2a, 2b, 2c) qui peuvent être appliquées sur la première partie hydrophile de surface (5a) entrent en contact avec des microgouttes (2a, 2b, 2c) qui peuvent être placées sur la deuxième partie hydrophile de surface (5b),
    caractérisé en ce que
    au moins un support (3a, 3b) présente une sonde d'humidité et/ou une sonde de conductivité.
  2. Dispositif selon la revendication 1, caractérisé en ce que la sonde d'humidité et/ou la sonde de conductivité sont disposées sur une partie hydrophile de surface du support (3a, 3b).
  3. Dispositif (1) selon les revendications 1 ou 2, caractérisé en ce qu'il présente au moins trois des supports structurés (3a, 3b) et en ce que ces supports (3a, 3b) peuvent être positionnés l'un contre l'autre de manière sélective ou alternée au moyen du dispositif de positionnement.
  4. Dispositif (1) selon l'une des revendications 1 à 3, caractérisé en ce qu'au moins un support (3a, 3b) présente un substrat d'oxyde de métal ou d'oxyde de semi-métal qui est revêtu sur les parties hydrophiles de surface par un polymère qui présente au moins un groupe réactif.
  5. Dispositif (1) selon l'une des revendications 1 à 4, caractérisé en ce que le dispositif de positionnement présente des éléments de centrage qui coopèrent l'un avec l'autre sur les supports (3a, 3b) qui doivent être placés l'un contre l'autre, et en particulier des biseaux de centrage.
  6. Dispositif (1) selon l'une des revendications 1 à 5, caractérisé en ce qu'au moins un support (3a, 3b) présente un élément de refroidissement ou un élément de chauffage et en particulier un élément Pelletier.
EP06010516A 2006-05-22 2006-05-22 Appareil pour mélanger des micro-gouttelettes Not-in-force EP1860060B1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
DE502006007372T DE502006007372D1 (de) 2006-05-22 2006-05-22 Vorrichtung zum Mischen von Mikrotröpfchen
EP06010516A EP1860060B1 (fr) 2006-05-22 2006-05-22 Appareil pour mélanger des micro-gouttelettes
US11/805,242 US20090017505A1 (en) 2006-05-22 2007-05-22 Process and device for mixing microdroplets

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP06010516A EP1860060B1 (fr) 2006-05-22 2006-05-22 Appareil pour mélanger des micro-gouttelettes

Publications (2)

Publication Number Publication Date
EP1860060A1 EP1860060A1 (fr) 2007-11-28
EP1860060B1 true EP1860060B1 (fr) 2010-07-07

Family

ID=37116094

Family Applications (1)

Application Number Title Priority Date Filing Date
EP06010516A Not-in-force EP1860060B1 (fr) 2006-05-22 2006-05-22 Appareil pour mélanger des micro-gouttelettes

Country Status (3)

Country Link
US (1) US20090017505A1 (fr)
EP (1) EP1860060B1 (fr)
DE (1) DE502006007372D1 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070047388A1 (en) * 2005-08-25 2007-03-01 Rockwell Scientific Licensing, Llc Fluidic mixing structure, method for fabricating same, and mixing method
ATE494061T1 (de) * 2007-07-10 2011-01-15 Hoffmann La Roche Mikrofluidische vorrichtung, mischverfahren und verwendung der vorrichtung
EP2228132A1 (fr) * 2009-03-10 2010-09-15 Qiagen GmbH Dispositif isotherme PCR
EP2760583B1 (fr) * 2011-09-30 2020-05-06 Life Technologies Corporation Systèmes pour une analyse biologique
CN105749995B (zh) * 2016-04-28 2017-10-31 宁波大学 一种生物芯片杂交装置
CN113713868B (zh) * 2021-09-13 2023-05-12 北京京东方技术开发有限公司 一种微流控制芯片及其制作方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3175843D1 (en) 1981-09-25 1987-02-26 Stocker Winfried Apparatus for photometric analyses
US6893877B2 (en) * 1998-01-12 2005-05-17 Massachusetts Institute Of Technology Methods for screening substances in a microwell array
WO1999039829A1 (fr) * 1998-02-04 1999-08-12 Merck & Co., Inc. Puits virtuels destines a etre utilises dans des criblages a haut rendement
DE19859623A1 (de) * 1998-12-23 2000-08-24 Basf Drucksysteme Gmbh Photopolymerisierbare Druckformen mit Oberschicht zur Herstellung von Reliefdruckformen
US20020151040A1 (en) * 2000-02-18 2002-10-17 Matthew O' Keefe Apparatus and methods for parallel processing of microvolume liquid reactions
US20040018615A1 (en) 2000-08-02 2004-01-29 Garyantes Tina K. Virtual wells for use in high throughput screening assays
US20020197733A1 (en) * 2001-06-20 2002-12-26 Coventor, Inc. Microfluidic system including a virtual wall fluid interface port for interfacing fluids with the microfluidic system
US20050019224A1 (en) 2003-06-16 2005-01-27 Schering Corporation Virtual well plate system

Also Published As

Publication number Publication date
US20090017505A1 (en) 2009-01-15
DE502006007372D1 (de) 2010-08-19
EP1860060A1 (fr) 2007-11-28

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