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EA200900096A1 - Способ получения полипептидов - Google Patents

Способ получения полипептидов

Info

Publication number
EA200900096A1
EA200900096A1 EA200900096A EA200900096A EA200900096A1 EA 200900096 A1 EA200900096 A1 EA 200900096A1 EA 200900096 A EA200900096 A EA 200900096A EA 200900096 A EA200900096 A EA 200900096A EA 200900096 A1 EA200900096 A1 EA 200900096A1
Authority
EA
Eurasian Patent Office
Prior art keywords
suitability
methods
codon pairs
individual codons
host cells
Prior art date
Application number
EA200900096A
Other languages
English (en)
Other versions
EA015925B1 (ru
Inventor
Йоханнес Андрис Раубос
Ноэль Николас Мария Элизабет Пейдж Ван
Original Assignee
ДСМ АйПи АССЕТС Б.В.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ДСМ АйПи АССЕТС Б.В. filed Critical ДСМ АйПи АССЕТС Б.В.
Publication of EA200900096A1 publication Critical patent/EA200900096A1/ru
Publication of EA015925B1 publication Critical patent/EA015925B1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C12N9/242—Fungal source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Настоящее изобретение касается способов оптимизации кодирующих белки последовательностей для экспрессии в заданных клетках хозяина. В этих способах для оптимизации последовательностей, кодирующих заданную аминокислотную последовательность, по пригодности отдельных кодонов и/или по пригодности пар кодонов применяются генетические алгоритмы. При создании новых вариантов последовательностей и последующем отборе наилучших вариантов алгоритм выполняет итерацию до тех пор, пока варианты кодирующих последовательностей не достигнут минимального значения пригодности отдельных кодонов и/или пригодности пар кодонов. Изобретение также касается компьютера, включающего процессор и запоминающее устройство (память), причем компьютер настроен на чтение из памяти и запись в неё, при этом память включает данные и инструкции, предназначенные для придания процессору способности к выполнению генетических алгоритмов для оптимизации пригодности отдельных кодонов и/или пригодности пар кодонов. Изобретение также касается нуклеиновых кислот, содержащих кодирующую последовательность для заданной аминокислотной последовательности, причем кодирующая последовательность оптимизирована по пригодности отдельных кодонов и/или по пригодности пар кодонов для данного хозяина способами изобретения, а также клеток хозяина, содержащих такие нуклеиновые кислоты, и способов получения полипептидов и других продуктов ферментации, в которых применяются эти клетки хозяина.
EA200900096A 2006-06-29 2007-06-15 Способ получения полипептидов EA015925B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP06076328 2006-06-29
PCT/EP2007/055943 WO2008000632A1 (en) 2006-06-29 2007-06-15 A method for achieving improved polypeptide expression

Publications (2)

Publication Number Publication Date
EA200900096A1 true EA200900096A1 (ru) 2009-06-30
EA015925B1 EA015925B1 (ru) 2011-12-30

Family

ID=37199000

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200900096A EA015925B1 (ru) 2006-06-29 2007-06-15 Способ получения полипептидов

Country Status (12)

Country Link
US (2) US8812247B2 (ru)
EP (2) EP2035561A1 (ru)
JP (1) JP5250850B2 (ru)
CN (1) CN101490262B (ru)
AU (1) AU2007263880A1 (ru)
BR (1) BRPI0713795B1 (ru)
CA (1) CA2657975A1 (ru)
DK (1) DK2423315T3 (ru)
EA (1) EA015925B1 (ru)
ES (1) ES2534282T3 (ru)
PL (1) PL2423315T3 (ru)
WO (1) WO2008000632A1 (ru)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013010057A1 (en) * 2011-07-13 2013-01-17 Aemetis, Inc. Compositons and methods for the production of isoprene

Families Citing this family (132)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10260805A1 (de) * 2002-12-23 2004-07-22 Geneart Gmbh Verfahren und Vorrichtung zum Optimieren einer Nucleotidsequenz zur Expression eines Proteins
AU2007281998B2 (en) * 2006-08-04 2014-02-20 Pharmathene Inc. Long half-life recombinant butyrylcholinesterase
AU2008214663B2 (en) 2007-02-15 2013-10-03 Dsm Ip Assets B.V. A recombinant host cell for the production of a compound of interest
WO2009005564A2 (en) * 2007-06-29 2009-01-08 The Regents Of The University Of California Cellulose- and hemicellulose-degradation enzyme -encoding nucleotide sequences with refined translational kinetics and methods of making same
WO2009030254A1 (en) * 2007-09-04 2009-03-12 Curevac Gmbh Complexes of rna and cationic peptides for transfection and for immunostimulation
EP2222854A1 (en) 2007-11-20 2010-09-01 DSM IP Assets B.V. Dicarboxylic acid production in a filamentous fungus
CN104818224A (zh) 2007-11-20 2015-08-05 帝斯曼知识产权资产管理有限公司 在真核细胞中生产琥珀酸
US20110104771A1 (en) * 2008-02-15 2011-05-05 Rene Verwall Process for the production of a dicarboxylic acid
AU2009221104B2 (en) 2008-03-07 2014-07-31 Dsm Ip Assets B.V. A pentose sugar fermenting cell
EA201001436A1 (ru) * 2008-03-07 2011-02-28 ДСМ АйПи АССЕТС Б.В. Клетка, сбраживающая пентозный сахар
EP2252698B1 (en) 2008-03-11 2017-11-22 Genomatica, Inc. Adipate ester or thioester synthesis
CN102026960B (zh) 2008-03-11 2014-07-16 帝斯曼知识产权资产管理有限公司 从5-甲酰缬草酸制备6-氨基己酸
EP2123768A1 (en) 2008-05-20 2009-11-25 DSM IP Assets B.V. Preparation of (Z)-6,7-dihydro-1H-azepin-2(5H)-one
EP2123767A1 (en) 2008-05-20 2009-11-25 DSM IP Assets B.V. Preparation of epsilon-caprolactam via lysine cyclisation
CA2730595C (en) 2008-07-08 2014-12-16 Dsm Ip Assets B.V. Low ph dicarboxylic acid production
EP2406372B1 (en) 2009-03-10 2017-08-23 DSM IP Assets B.V. Pregastric esterase and derivatives thereof
CN102741421B (zh) 2009-03-10 2015-03-25 帝斯曼知识产权资产管理有限公司 用于提高多肽产率的方法
AU2010221862A1 (en) 2009-03-11 2011-10-06 Dsm Ip Assets B.V. Preparation of alpha-ketopimelic acid
EP2419518A1 (en) 2009-04-15 2012-02-22 DSM IP Assets B.V. Dicarboxylic acid production process
WO2010121933A1 (en) 2009-04-22 2010-10-28 Dsm Ip Assets B.V. Process for the production of a recombinant polypeptide of interest
UA108853C2 (uk) 2009-07-10 2015-06-25 Спосіб ферментації галактози
EP3293264A1 (en) 2009-07-22 2018-03-14 DSM IP Assets B.V. Improved host cell for the production of a compound of interest
CA2768614A1 (en) 2009-07-24 2011-01-27 Dsm Ip Assets B.V. Process for the preparation of 1,4-butanediamine via n-acyl or n-guanidyl protected 1,4-butanediamine precursors
US9783834B2 (en) 2009-08-27 2017-10-10 Dsm Ip Assets B.V. Dicarboxylic acid fermentation process
CA2771828A1 (en) 2009-09-03 2011-03-10 Dsm Ip Assets B.V. Baking enzyme composition as ssl replacer
EP2504307A1 (en) 2009-11-24 2012-10-03 DSM IP Assets B.V. Process for the crystallization of succinic acid
EP2336310A1 (en) 2009-12-16 2011-06-22 Isobionics B.V. Valencene synthase
AU2010340358B2 (en) * 2009-12-21 2014-07-24 Pharmathene, Inc. Recombinant butyrylcholinesterases and truncates thereof
JP5521236B2 (ja) * 2009-12-22 2014-06-11 独立行政法人産業技術総合研究所 発現予測装置および発現予測方法
EP2534264A4 (en) * 2010-02-09 2014-02-26 Univ Columbia METHOD FOR CHANGING A POLYPEPTIDE EXPRESSION AND SOLUBILITY
WO2011157717A1 (en) 2010-06-14 2011-12-22 Dsm Fine Chemicals Austria Nfg. Gmbh & Co Kg Novel polypeptides having nad(p)h oxidase activity and the use thereof
DK2588616T3 (en) 2010-07-01 2019-03-11 Dsm Ip Assets Bv PROCEDURE FOR MAKING A RELATIONSHIP OF INTEREST
ES2650245T3 (es) 2010-07-14 2018-01-17 Dpx Holdings B.V. Método enzimático para aminación (R)-selectiva
WO2012031910A2 (en) 2010-09-10 2012-03-15 Dsm Ip Assets B.V. Method for preparing alpha-ketopimelic acid by c1-elongation
WO2012031911A2 (en) 2010-09-10 2012-03-15 Dsm Ip Assets B.V. Preparation of 6-aminocaproic acid from alpha-ketopimelic acid
BR112013006883A2 (pt) 2010-09-24 2016-06-07 Dsm Ip Assets Bv processo de produção de ácido dicarboxílico
EP2495304A1 (en) 2010-12-03 2012-09-05 DSM IP Assets B.V. Dicarboxylic acid production in a yeast cell
WO2012089613A1 (en) 2010-12-28 2012-07-05 Dsm Ip Assets B.V. Process to increase the production of a succinyl-coa derived compound
EP2683732B1 (en) 2011-03-11 2016-08-24 DSM IP Assets B.V. Vector-host system
TW201245448A (en) 2011-04-01 2012-11-16 Dsm Ip Assets Bv Preparation of 5-formyl valeric acid from alpha-ketopimelic acid
EP2537926A1 (en) 2011-06-21 2012-12-26 Isobionics B.V. Valencene synthase
CA2840773C (en) 2011-07-01 2019-09-03 Dsm Ip Assets B.V. Process for preparing dicarboxylic acids employing fungal cells
WO2013007820A1 (en) 2011-07-14 2013-01-17 Dsm Ip Assets B.V. Screening method
AR087423A1 (es) 2011-08-04 2014-03-19 Dsm Ip Assets Bv Celula capaz de fermentar azucares pentosas
EP2554668A1 (en) 2011-08-04 2013-02-06 DSM IP Assets B.V. A pentose sugar fermenting cell
CA2861697C (en) 2012-01-05 2022-03-15 Novartis Ag Protease deficient filamentous fungal cells and methods of use thereof
DK2620496T3 (en) 2012-01-30 2015-09-07 Dsm Ip Assets Bv Alpha-amylase
BR112014025693B1 (pt) 2012-04-17 2021-12-07 F. Hoffmann-La Roche Ag Método de produção recombinante de um polipeptídeo em uma célula bacteriana
US9695454B2 (en) 2012-05-23 2017-07-04 Glykos Finland Oy Production of fucosylated glycoproteins
US20150147365A1 (en) 2012-05-31 2015-05-28 Dsm Ip Assets B.V. Oral preparation
WO2014014339A2 (en) 2012-07-18 2014-01-23 Isobionics B.V. Rhodobacter for preparing terpenoids
WO2014013074A1 (en) 2012-07-19 2014-01-23 Dsm Ip Assets B.V. Agse-deficient strain
EP2877576B1 (en) * 2012-07-24 2019-06-05 BP Corporation North America Inc. Xylose isomerases and their uses
WO2014033019A1 (en) 2012-08-28 2014-03-06 Dsm Ip Assets B.V. Yeast strains engineered to produce ethanol from acetate
IN2015DN01041A (ru) 2012-08-28 2015-06-26 Dsm Ip Assets Bv
US9951339B2 (en) 2012-09-19 2018-04-24 Dsm Ip Assets B.V. Cell modification method using essential genes as markers and optionally recycling these
SG10201602115PA (en) * 2012-09-19 2016-05-30 Univ Singapore Codon optimization of a synthetic gene(s) for protein expression
EP2909305B1 (en) 2012-10-16 2018-12-26 DSM IP Assets B.V. Cells with improved pentose conversion
WO2014142647A1 (en) 2013-03-14 2014-09-18 Wageningen Universiteit Fungals strains with improved citric acid and itaconic acid production
DK3019602T3 (en) 2013-07-10 2018-11-12 Glykos Finland Oy MULTIPLE PROTEASE-DEFECTED FILAMENTARY FUNGAL CELLS AND PROCEDURES FOR USE THEREOF
EP3041507B1 (en) 2013-08-26 2021-06-30 BioNTech Research and Development, Inc. Nucleic acids encoding human antibodies to sialyl-lewis a
AR097480A1 (es) 2013-08-29 2016-03-16 Dsm Ip Assets Bv Células de levadura convertidoras de glicerol y ácido acético con una conversión de ácido acético mejorada
US10487125B2 (en) 2013-12-02 2019-11-26 Dsm Ip Assets B.V. Ice structuring protein
WO2015177152A1 (en) 2014-05-19 2015-11-26 Dsm Ip Assets B.V. Proline-specific endoprotease
WO2015177153A1 (en) 2014-05-19 2015-11-26 Dsm Ip Assets B.V. Proline-specific endoprotease
MX375798B (es) 2014-05-19 2025-03-07 Dsm Ip Assets Bv Endoproteasa específica para prolina.
AU2015271685B2 (en) 2014-06-04 2021-02-18 Biontech Research And Development, Inc. Human monoclonal antibodies to ganglioside GD2
US10513724B2 (en) 2014-07-21 2019-12-24 Glykos Finland Oy Production of glycoproteins with mammalian-like N-glycans in filamentous fungi
DK3242950T3 (da) 2015-01-06 2021-12-20 Dsm Ip Assets Bv Crispr-cas-system til en trådformet svampeværtscelle
DK3242948T3 (da) 2015-01-06 2022-03-07 Dsm Ip Assets Bv Crispr-cas-system til en yarrowia-værtscelle
EP3242949B1 (en) 2015-01-06 2021-11-03 DSM IP Assets B.V. A crispr-cas system for a yeast host cell
WO2016131818A1 (en) 2015-02-16 2016-08-25 Dsm Ip Assets B.V. Process for producing itaconic acid under anaerobic conditions
AR104205A1 (es) 2015-04-09 2017-07-05 Dsm Ip Assets Bv Fosfolipasa c
MX2017015126A (es) 2015-06-02 2018-03-28 Dsm Ip Assets Bv Uso de proteina estructurante del hielo.
US10724040B2 (en) 2015-07-15 2020-07-28 The Penn State Research Foundation mRNA sequences to control co-translational folding of proteins
WO2017050652A1 (en) 2015-09-25 2017-03-30 Dsm Ip Assets B.V. Asparaginase
EP4361240A3 (en) 2015-11-17 2024-07-31 DSM IP Assets B.V. Preparation of a stable beer
EP3469076B1 (en) 2016-06-10 2020-09-02 DSM IP Assets B.V. Mutant lipase and use thereof
EP3485004B1 (en) 2016-07-13 2021-09-22 DSM IP Assets B.V. Malate dehyrogenases
EP3484994A4 (en) 2016-07-13 2020-01-22 DSM IP Assets B.V. A crispr-cas system for an algal host cell
WO2018019948A1 (en) 2016-07-29 2018-02-01 Dsm Ip Assets B.V. Polypeptides having cellulolytic enhancing activity and uses thereof
CN106650307B (zh) * 2016-09-21 2019-04-05 武汉伯远生物科技有限公司 一种基于密码子对使用频度的基因密码子优化方法
WO2017216392A1 (en) 2016-09-23 2017-12-21 Dsm Ip Assets B.V. A guide-rna expression system for a host cell
US10918113B2 (en) 2016-12-21 2021-02-16 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114912A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
CA3046153A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114938A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
KR20190095429A (ko) 2016-12-22 2019-08-14 디에스엠 아이피 어셋츠 비.브이. 글루타티온 환원 효소
WO2018148761A1 (en) * 2017-02-13 2018-08-16 The Regents Of The University Of Colorado, A Body Corporate Compositions, methods and uses for multiplexed trackable genomically-engineered polypeptides
NL2018457B1 (en) 2017-03-02 2018-09-21 Isobionics B V Santalene Synthase
CN110431224A (zh) 2017-03-13 2019-11-08 帝斯曼知识产权资产管理有限公司 锌双核簇转录调控因子缺陷型菌株
CN107353327A (zh) * 2017-03-30 2017-11-17 南京百斯杰生物工程有限公司 植酸酶在黑曲霉中表达
WO2018127611A1 (en) 2017-04-06 2018-07-12 Dsm Ip Assets B.V. Self-guiding integration construct (sgic)
CN110070913B (zh) * 2017-07-25 2023-06-27 南京金斯瑞生物科技有限公司 一种基于免疫算法的密码子优化方法
NL2019473B1 (en) 2017-09-01 2019-03-11 Isobionics B V Terpene Synthase producing patchoulol and elemol, and preferably also pogostol
BR112020005972A2 (pt) 2017-09-26 2020-11-17 Bunge Global Innovation, Llc remoção enzimática de substratos de clorofila de óleos à base de triacilglicerol
US11707522B2 (en) 2017-10-13 2023-07-25 Boehringer Ingelheim International Gmbh Human antibodies to Tn antigen
WO2019012162A2 (en) 2017-12-20 2019-01-17 Dsm Ip Assets B.V. GENOMIC EDITING METHOD IN HOST CELL
US11944104B2 (en) 2018-04-05 2024-04-02 Dsm Ip Assets B.V. Variant maltogenic alpha-amylase
CN112088215A (zh) 2018-05-09 2020-12-15 帝斯曼知识产权资产管理有限公司 Crispr瞬时表达构建体(ctec)
US20230250406A1 (en) 2018-05-18 2023-08-10 Dsm Ip Assets B.V. Mutant lipase and use thereof
KR20210013585A (ko) 2018-05-18 2021-02-04 디에스엠 아이피 어셋츠 비.브이. 돌연변이체 리파아제 및 이의 용도
US12195769B2 (en) 2018-06-19 2025-01-14 Dsm Ip Assets B.V. Lipolytic enzyme variants
EP3830830A4 (en) * 2018-07-30 2022-05-11 Nanjing GenScript Biotech Co., Ltd. Codon optimization
US20210225455A1 (en) * 2018-08-15 2021-07-22 Zymergen Inc. Bioreachable prediction tool with biological sequence selection
CN112638419B (zh) 2018-11-02 2022-06-24 北京伟德杰生物科技有限公司 修饰的ctla4及其使用方法
EP3880835A1 (en) 2018-11-15 2021-09-22 DSM IP Assets B.V. Improved production of riboflavin
US20220056460A1 (en) 2018-12-05 2022-02-24 Dsm Ip Assets B.V. Crispr guide-rna expression strategies for multiplex genome engineering
EP3946720A1 (en) 2019-03-27 2022-02-09 Bunge Global Innovation, LLC. Silica adsorbent treatment for removal of chlorophyll derivatives from triacylglycerol-based oils
EP3947670A2 (en) * 2019-03-28 2022-02-09 Intellia Therapeutics, Inc. Polynucleotides, compositions, and methods for polypeptide expression
BR112021025806A2 (pt) 2019-06-28 2022-02-08 Hoffmann La Roche Métodos para produzir um anticorpo da subclasse igg1 humana cultivando uma célula cho e uso da remoção de sítios de splice de doador não pareados em uma parte de uma sequência de ácido nucleico de humano ou hamster
EP3766982A1 (en) 2019-07-18 2021-01-20 Delft Advanced Biofuels B.V. Integrated system for biocatalytically producing and recovering an organic substance
CN110491447B (zh) * 2019-08-05 2021-08-17 浙江省农业科学院 一种用于异源基因体外表达的密码子优化方法及应用
CN110873833B (zh) * 2019-12-10 2022-04-08 国网河南省电力公司洛阳供电公司 一种含分布式电源的配电网自适应故障区段定位方法
WO2021180702A1 (en) 2020-03-10 2021-09-16 Dsm Ip Assets B.V. Lactose reduced dairy powder
EP4015626A1 (en) 2020-12-18 2022-06-22 Isobionics B.V. Enzymes and methods for fermentative production of monoterpene esters
JP2022155412A (ja) * 2021-03-30 2022-10-13 国立大学法人 奈良先端科学技術大学院大学 植物へ導入する遺伝子の人工配列の設計システム
WO2022223010A1 (zh) 2021-04-21 2022-10-27 浙江大学 一种负链rna病毒载体及无需转化的植物基因组编辑方法
WO2022263504A1 (en) 2021-06-17 2022-12-22 Dsm Ip Assets B.V. Improved bacillus host cell
JP2024528957A (ja) 2021-08-02 2024-08-01 ビーエーエスエフ ソシエタス・ヨーロピア イオニリデンエタンシンターゼによる新規な芳香化合物の生成
JP2024539457A (ja) 2021-11-19 2024-10-28 ミロバイオ・リミテッド 操作されたｐｄ－１抗体及びその使用
AU2023251091A1 (en) 2022-04-06 2024-10-10 Gilead Sciences, Inc. Engineered cd200r antibodies and uses thereof
EP4525615A2 (en) 2022-05-14 2025-03-26 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
WO2023222614A1 (en) 2022-05-16 2023-11-23 Dsm Ip Assets B.V. Lipolytic enzyme variants
DE102022118459A1 (de) 2022-07-22 2024-01-25 Proteolutions UG (haftungsbeschränkt) Verfahren zur optimierung einer nukleotidsequenz für die expression einer aminosäuresequenz in einem zielorganismus
EP4567818A4 (en) * 2022-09-09 2025-12-17 Nanjing Genscript Biotech Co Ltd METHOD FOR DESIGNING A SEQUENCE OF HUMANIZED ANTIBODY
EP4365192A1 (en) 2022-11-04 2024-05-08 DSM IP Assets B.V. Microbial production of growth factors
WO2025012325A1 (en) 2023-07-11 2025-01-16 Dsm Ip Assets B.V. Protein arginine deiminase
WO2025099225A1 (en) 2023-11-08 2025-05-15 Dsm Ip Assets B.V. Improved enzyme variants
WO2025099224A1 (en) 2023-11-08 2025-05-15 Dsm Ip Assets B.V. Improved enzyme variants
WO2025144785A2 (en) 2023-12-28 2025-07-03 Dsm Ip Assets B.V. Cells and methods for the production of glycosylated compounds
WO2026003190A1 (en) 2024-06-27 2026-01-02 Basf Se Means and methods for alpha-farnesene production

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
EP0340878B1 (en) 1988-05-06 2001-05-16 Aventis Pharma S.A. Process for the biochemical oxidation of steroids and genetically engineered cells to be used therefor
CA1333777C (en) 1988-07-01 1995-01-03 Randy M. Berka Aspartic proteinase deficient filamentous fungi
US5082767A (en) * 1989-02-27 1992-01-21 Hatfield G Wesley Codon pair utilization
ATE238425T1 (de) 1993-07-23 2003-05-15 Dsm Nv Selektionmarker-genfreie rekombinante stämme: verfahren zur ihrer herstellung und die verwendung dieser stämme
US5795737A (en) 1994-09-19 1998-08-18 The General Hospital Corporation High level expression of proteins
CN1611601A (zh) 1994-11-08 2005-05-04 诺沃奇梅兹有限公司 三肽基氨肽酶
US5874304A (en) * 1996-01-18 1999-02-23 University Of Florida Research Foundation, Inc. Humanized green fluorescent protein genes and methods
AU2152897A (en) 1996-03-27 1997-10-17 Novo Nordisk A/S Alkaline protease deficient filamentous fungi
CN1169961C (zh) 1997-04-11 2004-10-06 Dsm公司 基因转变作为工具用于构建重组的工业化丝状真菌
CA2296067C (en) * 1997-07-09 2008-10-07 The University Of Queensland Nucleic acid sequence and method for selectively expressing a protein in a target cell or tissue
DK1042461T3 (da) 1997-12-22 2007-09-24 Dsm Ip Assets Bv Ekspressionskloning i filiforme svampe
ATE414782T1 (de) 1998-12-23 2008-12-15 Novozymes As Verfahren zur herstellung von polypeptiden in mutierten aspergillus zellen
CN1195058C (zh) 1999-02-22 2005-03-30 诺维信公司 草酰乙酸水解酶缺陷型真菌宿主细胞
DE60142226D1 (de) 2000-03-14 2010-07-08 Novozymes As Pilz transkriptionsaktivator zur verwendung in verfahren zur herstellung von polypeptiden
DK1266018T3 (da) 2000-03-24 2008-09-01 Genencor Int Fremstilling af udskilte proteiner af rekombinante, eukaryotiske celler
EP1346044A2 (en) 2000-12-22 2003-09-24 Janssen Pharmaceutica N.V. Bax-responsive genes for drug target identification in yeast and fungi
US6749945B2 (en) 2001-01-29 2004-06-15 The Board Of Regents For Oklahoma State University Advanced composite ormosil coatings
EP1231272A3 (en) 2001-02-08 2002-11-13 Laboratorios Ovejero S.A. Procedure for obtaining bovine chymosin (rennin), curd
AU2003206684A1 (en) 2002-02-20 2003-09-09 Novozymes A/S Plant polypeptide production
AU2003228440B2 (en) 2002-04-01 2008-10-02 Walter Reed Army Institute Of Research Method of designing synthetic nucleic acid sequences for optimal protein expression in a host cell
WO2004070022A2 (en) 2003-02-05 2004-08-19 Dsm Ip Assets B.V. Use of oxalate deficient aspergillus niger strains for producing a polypeptide
CN101629204A (zh) 2003-03-31 2010-01-20 诺维信股份有限公司 在缺乏酶的黑曲霉突变体中生产生物物质的方法
WO2005095624A2 (en) 2004-04-02 2005-10-13 Dsm Ip Assets B.V. Filamentous fungal mutants with improved homologous recombination efficiency
EP1776461A2 (en) 2004-04-16 2007-04-25 DSMIP Assets B.V. Fungal promoters for expressing a gene in a fungal cell
DK1756145T3 (da) 2004-06-16 2014-09-29 Dsm Ip Assets Bv Produktion af polypeptider ved hjælp af forbedret sekretion
US7794974B2 (en) 2004-10-12 2010-09-14 Dsm Ip Assets B.V. Fungal transcriptional activators useful in methods for producing a polypeptide
CA2580884A1 (en) 2004-10-15 2006-04-20 Dsm Ip Assets B.V. Method for production of a compound in a eukaryotic cell
JP2008527985A (ja) * 2005-01-24 2008-07-31 ディーエスエム アイピー アセッツ ビー．ブイ． 糸状菌細胞において目的の化合物を産生させるための方法

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013010057A1 (en) * 2011-07-13 2013-01-17 Aemetis, Inc. Compositons and methods for the production of isoprene
CN103797112A (zh) * 2011-07-13 2014-05-14 阿梅蒂斯公司 用于异戊二烯生产的组合物和方法

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