EA000703B1 - Способ дезинтеграции культивированных клеток с использованием устройства на основе применения сталкивающихся струй - Google Patents
Способ дезинтеграции культивированных клеток с использованием устройства на основе применения сталкивающихся струй Download PDFInfo
- Publication number
- EA000703B1 EA000703B1 EA199800266A EA199800266A EA000703B1 EA 000703 B1 EA000703 B1 EA 000703B1 EA 199800266 A EA199800266 A EA 199800266A EA 199800266 A EA199800266 A EA 199800266A EA 000703 B1 EA000703 B1 EA 000703B1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- cells
- virus
- animal
- cell
- pressure
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 65
- 210000004748 cultured cell Anatomy 0.000 title claims abstract description 7
- 210000004027 cell Anatomy 0.000 claims abstract description 81
- 241000700605 Viruses Species 0.000 claims abstract description 33
- 210000004102 animal cell Anatomy 0.000 claims abstract description 28
- 239000012530 fluid Substances 0.000 claims abstract description 13
- 210000002421 cell wall Anatomy 0.000 claims abstract description 10
- 239000000725 suspension Substances 0.000 claims abstract description 8
- 238000012258 culturing Methods 0.000 claims abstract description 5
- 210000005265 lung cell Anatomy 0.000 claims abstract description 4
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 claims abstract description 3
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 claims abstract description 3
- 210000004978 chinese hamster ovary cell Anatomy 0.000 claims abstract description 3
- 210000002950 fibroblast Anatomy 0.000 claims abstract description 3
- 239000001963 growth medium Substances 0.000 claims abstract description 3
- 210000003501 vero cell Anatomy 0.000 claims abstract description 3
- 239000012531 culture fluid Substances 0.000 claims abstract 2
- 239000007788 liquid Substances 0.000 claims description 17
- 230000001413 cellular effect Effects 0.000 claims description 4
- 241000701085 Human alphaherpesvirus 3 Species 0.000 claims description 3
- 241001465754 Metazoa Species 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 abstract description 3
- 108090000623 proteins and genes Proteins 0.000 abstract description 3
- 238000003306 harvesting Methods 0.000 abstract 3
- 208000032767 Device breakage Diseases 0.000 abstract 1
- 230000006378 damage Effects 0.000 description 6
- 201000006082 Chickenpox Diseases 0.000 description 5
- 206010046980 Varicella Diseases 0.000 description 5
- 238000009210 therapy by ultrasound Methods 0.000 description 5
- 230000008901 benefit Effects 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 230000000254 damaging effect Effects 0.000 description 3
- 238000011031 large-scale manufacturing process Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000000240 pulsed field-gradient spin echo nuclear magnetic resonance spectroscopy Methods 0.000 description 3
- 230000035939 shock Effects 0.000 description 3
- 229960005486 vaccine Drugs 0.000 description 3
- 230000003612 virological effect Effects 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 208000007514 Herpes zoster Diseases 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 230000001066 destructive effect Effects 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000002458 infectious effect Effects 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 238000000053 physical method Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 210000001938 protoplast Anatomy 0.000 description 2
- 239000003381 stabilizer Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 238000010257 thawing Methods 0.000 description 2
- 235000011331 Brassica Nutrition 0.000 description 1
- 241000219198 Brassica Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000208125 Nicotiana Species 0.000 description 1
- 241001534530 Okavirus Species 0.000 description 1
- 240000007377 Petunia x hybrida Species 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 241000702670 Rotavirus Species 0.000 description 1
- 229940124924 Varivax Drugs 0.000 description 1
- 241000209149 Zea Species 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000010924 continuous production Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 210000001840 diploid cell Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000005847 immunogenicity Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000010297 mechanical methods and process Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 230000006911 nucleation Effects 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 238000003921 particle size analysis Methods 0.000 description 1
- 230000021715 photosynthesis, light harvesting Effects 0.000 description 1
- -1 polypropylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
- C12N1/066—Lysis of microorganisms by physical methods
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/16011—Herpesviridae
- C12N2710/16711—Varicellovirus, e.g. human herpesvirus 3, Varicella Zoster, pseudorabies
- C12N2710/16751—Methods of production or purification of viral material
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Sustainable Development (AREA)
- Mycology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US506295P | 1995-10-06 | 1995-10-06 | |
| GBGB9603759.3A GB9603759D0 (en) | 1996-02-22 | 1996-02-22 | Method of disrupting cultured cells using an impinging jet device |
| PCT/US1996/015801 WO1997012959A1 (en) | 1995-10-06 | 1996-10-02 | Method of disrupting cultured cells using an impinging jet device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EA199800266A1 EA199800266A1 (ru) | 1998-10-29 |
| EA000703B1 true EA000703B1 (ru) | 2000-02-28 |
Family
ID=26308786
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EA199800266A EA000703B1 (ru) | 1995-10-06 | 1996-10-02 | Способ дезинтеграции культивированных клеток с использованием устройства на основе применения сталкивающихся струй |
Country Status (10)
| Country | Link |
|---|---|
| EP (1) | EP0854913A1 (es) |
| CN (1) | CN1100868C (es) |
| AR (1) | AR003776A1 (es) |
| AU (1) | AU7204496A (es) |
| BR (1) | BR9610773A (es) |
| CZ (1) | CZ104198A3 (es) |
| EA (1) | EA000703B1 (es) |
| SK (1) | SK43498A3 (es) |
| TW (1) | TW426733B (es) |
| WO (1) | WO1997012959A1 (es) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2615448C2 (ru) * | 2010-12-28 | 2017-04-04 | Чугаи Сейяку Кабусики Кайся | Описание способа культивирования животной клетки |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19749735C1 (de) * | 1997-11-11 | 2000-02-10 | Invent Gmbh Entwicklung Neuer Technologien | Verfahren und Vorrichtung zur Herstellung von Enzymen |
| NZ533586A (en) | 2001-12-20 | 2005-02-25 | Bavarian Nordic As | Method for the recovery and purification of poxviruses from infected cells using high pressure homogenisation |
| DE102005034629B4 (de) * | 2005-07-19 | 2007-09-13 | Dr. Hielscher Gmbh | Vorrichtung und Verfahren zum mechanischen Aufschluss von Zellen |
| CN101522884B (zh) * | 2006-08-15 | 2013-08-21 | 巴斯夫欧洲公司 | 从生产细胞中分离蛋白质的方法 |
| CN104861038B (zh) * | 2015-05-08 | 2019-10-01 | 永联生物科技(上海)有限公司 | 一种均质阀组 |
| BR102018004973B1 (pt) * | 2018-03-13 | 2021-10-13 | Petróleo Brasileiro S.A. - Petrobras | Dispositivo e método para rompimento de células de microorganismos por extrusão |
| EP4296352A1 (en) | 2022-06-20 | 2023-12-27 | LenioBio GmbH | Tunable disruption of eukaryotic protoplast to release intact cellular organelles |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR346862A (fr) * | 1904-10-07 | 1905-02-13 | Henri Charles Empis | Extraction et préparation des substances contenues dans les cellules organiques |
| GB877898A (en) * | 1956-12-06 | 1961-09-20 | Apv Co Ltd | A new or improved method of liberating enzymes |
| US3309032A (en) * | 1964-03-23 | 1967-03-14 | Sorvall Inc Ivan | Cell fractionator apparatus |
| DE3810462A1 (de) * | 1988-03-26 | 1989-10-05 | Dechema | Verfahren zum aufschluss von zellen in einer zellsuspension und vorrichtung zur durchfuehrung des verfahrens |
-
1996
- 1996-10-01 TW TW085111939A patent/TW426733B/zh not_active IP Right Cessation
- 1996-10-02 CZ CZ981041A patent/CZ104198A3/cs unknown
- 1996-10-02 SK SK434-98A patent/SK43498A3/sk unknown
- 1996-10-02 EA EA199800266A patent/EA000703B1/ru not_active IP Right Cessation
- 1996-10-02 WO PCT/US1996/015801 patent/WO1997012959A1/en not_active Ceased
- 1996-10-02 AU AU72044/96A patent/AU7204496A/en not_active Abandoned
- 1996-10-02 BR BR9610773A patent/BR9610773A/pt not_active Application Discontinuation
- 1996-10-02 EP EP96933226A patent/EP0854913A1/en not_active Withdrawn
- 1996-10-02 AR ARP960104582A patent/AR003776A1/es unknown
- 1996-10-02 CN CN96198826A patent/CN1100868C/zh not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2615448C2 (ru) * | 2010-12-28 | 2017-04-04 | Чугаи Сейяку Кабусики Кайся | Описание способа культивирования животной клетки |
Also Published As
| Publication number | Publication date |
|---|---|
| AU7204496A (en) | 1997-04-28 |
| TW426733B (en) | 2001-03-21 |
| CZ104198A3 (cs) | 1998-08-12 |
| EP0854913A1 (en) | 1998-07-29 |
| CN1203626A (zh) | 1998-12-30 |
| CN1100868C (zh) | 2003-02-05 |
| AR003776A1 (es) | 1998-09-09 |
| WO1997012959A1 (en) | 1997-04-10 |
| SK43498A3 (en) | 1998-12-02 |
| EA199800266A1 (ru) | 1998-10-29 |
| BR9610773A (pt) | 1999-07-13 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s) |
Designated state(s): AM AZ BY KZ KG MD TJ TM |
|
| MM4A | Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s) |
Designated state(s): RU |